High anti-SARS-CoV-2 antibody seroconversion rates before the second wave in Manaus, Brazil, and the protective effect of social behaviour measures: results from the prospective DETECTCoV-19 cohort

Background: The city of Manaus, Brazil, has seen two collapses of the health system due to the COVID-19 pandemic. We report anti-SARS-CoV-2 nucleocapsid IgG antibody seroconversion rates and associated risk factors in Manaus residents before the second wave of the epidemic in Brazil. Methods: A convenience sample of adult (aged ≥18 years) residents of Manaus was recruited through online and university website advertising into the DETECTCoV-19 study cohort. The current analysis of seroconversion included a subgroup of DETECTCoV-19 participants who had at least two serum sample collections separated by at least 4 weeks between Aug 19 and Oct 2, 2020 (visit 1), and Oct 19 and Nov 27, 2020 (visit 2). Those who reported (or had no data on) having a COVID-19 diagnosis before visit 1, and who were positive for anti-SARS-CoV-2 nucleocapsid IgG antibodies at visit 1 were excluded. Using an in-house ELISA, the reactivity index (RI; calculated as the optical density ratio of the sample to the negative control) for serum anti-SARS-CoV-2 nucleocapsid IgG antibodies was measured at both visits. We calculated the incidence of seroconversion (defined as RI values ≤1·5 at visit 1 and ≥1·5 at visit 2, and a ratio >2 between the visit 2 and visit 1 RI values) during the study period, as well as incidence rate ratios (IRRs) through cluster-corrected and adjusted Poisson regression models to analyse associations between seroconversion and variables related to sociodemographic characteristics, health access, comorbidities, COVID-19 exposure, protective behaviours, and symptoms. Findings: 2496 DETECTCoV-19 cohort participants returned for a follow-up visit between Oct 19 and Nov 27, 2020, of whom 204 reported having COVID-19 before the first visit and 24 had no data regarding previous disease status. 559 participants were seropositive for anti-SARS-CoV-2 nucleocapsid IgG antibodies at baseline. Of the remaining 1709 participants who were seronegative at baseline, 71 did not meet the criteria for seroconversion and were excluded from the analyses. Among the remaining 1638 participants who were seronegative at baseline, 214 showed seroconversion at visit 2. The seroconversion incidence was 13·06% (95% CI 11·52–14·79) overall and 6·78% (5·61–8·10) for symptomatic seroconversion, over a median follow-up period of 57 days (IQR 54–61). 48·1% of seroconversion events were estimated to be asymptomatic. The sample had higher proportions of affluent and higher-educated people than those reported for the Manaus city population. In the fully adjusted and corrected model, risk factors for seroconversion before visit 2 were having a COVID-19 case in the household (IRR 1·49 [95% CI 1·21–1·83]), not wearing a mask during contact with a person with COVID-19 (1·25 [1·09–1·45]), relaxation of physical distancing (1·31 [1·05–1·64]), and having flu-like symptoms (1·79 [1·23–2·59]) or a COVID-19 diagnosis (3·57 [2·27–5·63]) between the first and second visits, whereas working remotely was associated with lower incidence (0·74 [0·56–0·97]). Interpretation: An intense infection transmission period preceded the second wave of COVID-19 in Manaus. Several modifiable behaviours increased the risk of seroconversion, including non-compliance with non-pharmaceutical interventions measures such as not wearing a mask during contact, relaxation of protective measures, and non-remote working. Increased testing in high-transmission areas is needed to provide timely information about ongoing transmission and aid appropriate implementation of transmission mitigation measures. Funding: Ministry of Education, Brazil; Fundação de Amparo à Pesquisa do Estado do Amazonas; Pan American Health Organization (PAHO)/WHO.World Health OrganizationRevisión por pare

Percepção de fisioterapeutas intensivistas após a implementação de um plano terapêutico voltado para pacientes críticos

Objective: To verify the perception of intensive care physiotherapists after the implementation of a Therapeutic Plan aimed at critical patients. Methodology: Cross-sectional and observational research. After the implementation of the Therapeutic Plan in the Intensive Care Units of public and private hospitals, a structured structured questionnaire containing 11 questions about the Therapeutic Plan was used. Result: The sample consisted of 38 participants, mostly female (68.4%). Physiotherapists had a positive perception of the Therapeutic Plan after its implementation in the Intensive Care Units, with no statistical difference between the private and public networks regarding this tool being effective for early rehabilitation in the service (p = 1,000) and in the clinical outcomes favorable to patients (p = 0.109). Conclusion: After implementing a Therapeutic Plan in an Intensive Care Unit aimed at critically ill patients, there was a positive perception of this device by physiotherapists in the management and outcome of patients during care practice.Objetivo: Verificar a percepção de fisioterapeutas intensivistas após a implementação de um Plano Terapêutico voltado para pacientes críticos. Metodologia: Pesquisa do tipo transversal e observacional. Após a implantação do Plano Terapêutico nas Unidades de Terapia Intensiva de hospitais público e privado foi utilizado questionário estruturado do tipo fechado contendo 11 perguntas sobre Plano Terapêutico. Resultado: A amostra foi constituída por 38 participantes, maioria do sexo feminino (68,4%). Os fisioterapeutas tiveram percepção positiva do Plano Terapêutico após sua implementação nas Unidades de Terapia Intensiva não havendo diferença estatística entre rede privada e pública com relação a esta ferramenta ser eficaz para uma reabilitação precoce no serviço (p= 1.000) e nos desfechos clínicos favoráveis aos pacientes (p= 0.109). Conclusão: Após implementação de um Plano Terapêutico em Unidade de Terapia Intensiva voltado para pacientes críticos, houve percepção positiva desse dispositivo pelos profissionais Fisioterapeutas no manejo e desfecho dos pacientes durante a prática assistencial.Objetivo: Verificar la percepción de los fisioterapeutas de cuidados intensivos luego de la implementación de un Plan Terapéutico dirigido a pacientes críticos. Metodología: Investigación transversal y observacional. Luego de la implementación del Plan Terapéutico en las Unidades de Cuidados Intensivos de hospitales públicos y privados, se utilizó un cuestionario estructurado estructurado que contiene 11 preguntas sobre el Plan Terapéutico. Resultado: La muestra estuvo formada por 38 participantes, en su mayoría mujeres (68,4%). Los fisioterapeutas tuvieron una percepción positiva del Plan Terapéutico luego de su implementación en las Unidades de Cuidados Intensivos, sin diferencia estadística entre las redes privadas y públicas en cuanto a que esta herramienta sea efectiva para la rehabilitación temprana en el servicio (p = 1,000) y en los resultados clínicos favorables a los pacientes (p = 0,109). Conclusión: luego de implementar un Plan Terapéutico en una Unidad de Cuidados Intensivos dirigido a pacientes críticos, hubo una percepción positiva de este dispositivo por parte de los fisioterapeutas en el manejo y evolución de los pacientes durante la práctica asistencia

QUALITY OF COMMERCIAL SOYBEAN GRAIN AND POST-HARVEST ECONOMIC LOSSES IN FARMING IN WEST PARÁ, BRAZIL

The soybean production chain is one of the main agribusiness products. In this context, the grain market is constantly expanding. Thus, it is important to carry out the characterization of soybeans and define their qualities. The objective of this study was to evaluate the quality of commercial soybean grains in postharvest in a field in the municipality of Mojuí dos Campos, Pará. Two types of cultivars at 4280(IPRO) and 8644(IPRO), harvested on different days were used. and parcels. Data collection was carried out by monitoring the post-harvest process. The information regarding the grains was obtained through the classification report provided by the company accredited to classify the grains. The methods used were carried out in accordance with the Technical Regulation of Soybeans of Normative Instruction No. 11, of May 15, 2007, of the Ministry of Agriculture, Livestock and Supply (MAPA). Data were analyzed by PROC TTEST in SAS version 19.0 at a significance level of 5%. The results showed that, in the period of harvesting of cultivar 8644(IPRO), there was a higher rate of rainfall compared to the days of harvesting of cultivar 4280(IPRO). The total of discounted grains was 15.101t for the cultivar 4280(IPRO). The wet grain was the only parameter responsible for the discount in both cultivars. The total of discounted grains referring to cultivar 8644(IPRO) was 30.218t. Wet grains had the highest classification rate on the fourth day of harvest, with a total of 23.20%, related to the decrease in the percentage of broken grains (1.60%) and a higher index of burnt/damaged grains. It is concluded that the cultivar 4280(IPRO) presented a lower grain discount compared to the cultivar 8644(IPRO). Consequently, cultivar 8644 presented greater economic damage to the property.A cadeia produtiva da soja é um dos principais produtos do agronegócio. Nesse contexto, o mercado de grãos está em constante expansão. Assim, faz-se importante realizar a caracterização dos grãos de soja e definir as suas qualidades. O objetivo neste estudo foi avaliar a qualidade de grãos comerciais de soja na pós-colheita em uma lavoura no município de Mojuí dos Campos, Pará. Foram utilizados dois tipos de cultivares a 4280(IPRO) e 8644(IPRO), colhidas em diferentes dias e parcelas. A coleta dos dados foi realizada por meio do acompanhamento do processo de pós-colheita. As informações referentes aos grãos foram obtidas por meio do Laudo de classificação fornecido pela empresa credenciada a classificar os grãos. Os métodos empregados foram realizados conforme o Regulamento Técnico da Soja da Instrução Normativa Nº 11, de 15 de maio de 2007, do Ministério da Agricultura, Pecuária e Abastecimento (MAPA). Os dados foram analisados pelo PROC TTEST no SAS versão 19.0 a um nível de significância de 5%. Os resultados mostraram que, no período de colheita da cultivar 8644(IPRO), houve um maior índice de precipitação pluvial em comparação com os dias de colheita da cultivar 4280(IPRO). O total de grãos descontados foi de 15,101t para a cultivar 4280(IPRO). O grão úmido o único parâmetro responsável pelo desconto em ambas as cultivares. O total de grãos descontados referentes a cultivar 8644(IPRO) foi de 30,218t. Os grãos úmidos obtiveram maior taxa de classificação no quarto dia da colheita com total de 23,20%, tendo relação com a diminuição da porcentagem dos grãos quebrados (1,60%) e um maior índice de grãos ardidos/avariados. Conclui-se que a cultivar 4280(IPRO), apresentou menor desconto de grãos em comparação com a cultivar 8644(IPRO). Por consequência a cultivar 8644 apresentou maior dano econômico à propriedade

Behavior of buffalo heifers reared in shaded and unshaded pastures during the dry season on Marajó Island, Pará, Brazil

The objective of this research was to evaluate the behavior of twenty buffalo heifers reared during the dry season on Marajó Island, Pará, Brazil. The animals were distributed into two groups, those with shade (WS) and those without shade (WOS). The experimental day was divided into six shifts: morning (6:00 to 9:55 a.m.), intermediate (10:00 a.m. to 1:55 p.m.), afternoon (2:00 to 5:55 p.m.), evening (6:00 to 9:55 p.m.), night (10:00 p.m. to 1:55 a.m.), and early morning (2:00 to 5:55 a.m.). The WS group was kept in silvipastoral system paddocks, while the WOS group was kept in unshaded paddocks. Climatic data were registered and temperature and humidity index (THI) were calculated. Behavior data such as grazing, rumination, idle time, and other activities (walking, defecating, mounting, drinking water, urinating, eating salt) were evaluated. The results showed that the THI was higher in the WOS group. Grazing time was higher in the WOS group than in the WS group in the intermediate shift (p < 0.05), while in the afternoon, evening, and early morning shifts, the WS group spent more time grazing than the WOS group. In the intermediate and early morning shifts, the WS group spent more time ruminating (p < 0.05); the WS group ruminated more than the WOS group. The WOS group had more idle time, especially in the evening and early morning shifts (p < 0.05), while the WS group had more idle time in the intermediate shift (p < 0.05). We conclude that buffaloes graze and ruminate more intensely when they are reared in a shaded system on the island of Marajó

Mixed Models in Nonlinear Regression for Description of the Growth of Nelore Cattle

Body weight records were used to characterize the growth curve of Nelore cattle. Body weight was regressed as a function of age, for both sexes, by using nonlinear models through the functions of Brody, Gompertz, Logistic, Richards, Meloun 1, Von Bertalanffy, and Von Bertalanffy. The quality of the model arrangements was evaluated by employing Akaike and Bayesian Schwarz information criteria. The Brody function provided the best adaptations by the evaluators and, considering the asymptotic weight and the maturation rate as random, a reduction in residual variance of 79% for males and 83% for females was obtained in relation to the models under fixed contexts. In males, the absolute and relative growth rates ranged from 0.921 to 0.261 kg/day and 2.39 to 0.08%, respectively. For the same rates, under another approach, females ranged from 0.922 to 0.198 kg/day and 2.55 to 0.06%, respectively. Males showed greater growth acceleration at the beginning of the growth trajectory, being equal to females at 397 days of age and from that age onward they presented lower estimates. The nonlinear regression model approach under the mixed-models context allows reduction of residual variance, increasing model accuracy

Perspectives of economic losses due to condemnation of cattle and buffalo carcasses in the northern region of Brazil.

The work aims to study the economical losses of the condemnation of bovine and buffalo carcasses, in order to estimate the losses in animals slaughtered in Santarém-Pará, Brazil, between 2016 and 2018, with data obtained from the Municipal Department of Agriculture and Fisheries. Sex, age, origin, total number of animals slaughtered and causes of condemnation of carcasses were considered. All analyzes were performed in RStudio version 1.1.463. In this study, 71,277 bovine carcasses and 2,016 buffalo carcasses were inspected, of which 300 bovine and 71 buffalo were condemned. The highest prevalence of causes of condemnation in cattle was recorded for brucellosis (0.0020%) and tuberculosis (0.0019%). In buffaloes, tuberculosis (0.0307%) peritonitis (0,0019%) were the main causes of condemnations. Economical losses were more evident in females, for both species. The projection of economical losses related to the condemnation of carcasses showed a sharp growth for the next three years, if the average growth remains constant. The biggest projected loss was for bovine females, with an accumulated projection of $ 5,451.44. The smallest estimated loss was for buffalo males, projected at more than thirty-two thousand reais. The most important causes of condemnation report the diseases brucellosis and tuberculosis, as the ones with the greatest impact. In the buffalo species this was even more accentuated, even though the number of buffaloes slaughtered is more than 35 times smaller than the number of cattle

Núcleos de Ensino da Unesp: artigos 2008

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq