186 research outputs found
A Field Trial of Alternative Targeted Screening Strategies for Chagas Disease in Arequipa, Peru
In the wake of emerging T. cruzi infection in children of periurban Arequipa, Peru, we conducted a prospective field trial to evaluate alternative targeted screening strategies for Chagas disease across the city. Using insect vector data that is routinely collected during Ministry of Health insecticide application campaigns in 3 periurban districts of Arequipa, we separated into 4 categories those households with 1) infected vectors; 2) high vector densities; 3) low vector densities; and 4) no vectors. Residents of all infected-vector households and a random sample of those in the other 3 categories were invited for serological screening for T. cruzi infection. Subsequently, all residents of households within a 15-meter radius of detected seropositive individuals were invited to be screened in a ring case-detection scheme. Of 923 participants, 21 (2.28%) were seropositive. There were no significant differences in prevalence across the 4 screening strategies, indicating that household entomologic factors alone could not predict the risk of infection. Indeed, the most predictive variable of infection was the number of years a person lived in a location with triatomine insects. Therefore, a simple residence history questionnaire may be a useful screening tool in large, diverse urban environments with emerging Chagas disease
IFNAR1-Signalling Obstructs ICOS-mediated Humoral Immunity during Non-lethal Blood-Stage Plasmodium Infection
Funding: This work was funded by a Career Development Fellowship (1028634) and a project grant (GRNT1028641) awarded to AHa by the Australian National Health & Medical Research Council (NHMRC). IS was supported by The University of Queensland Centennial and IPRS Scholarships. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD
Horizontal DNA transfer mechanisms of bacteria as weapons of intragenomic conflict
Horizontal DNA transfer (HDT) is a pervasive mechanism of diversification in many microbial species, but its primary evolutionary role remains controversial. Much recent research has emphasised the adaptive benefit of acquiring novel DNA, but here we argue instead that intragenomic conflict provides a coherent framework for understanding the evolutionary origins of HDT. To test this hypothesis, we developed a mathematical model of a clonally descended bacterial population undergoing HDT through transmission of mobile genetic elements (MGEs) and genetic transformation. Including the known bias of transformation toward the acquisition of shorter alleles into the model suggested it could be an effective means of counteracting the spread of MGEs. Both constitutive and transient competence for transformation were found to provide an effective defence against parasitic MGEs; transient competence could also be effective at permitting the selective spread of MGEs conferring a benefit on their host bacterium. The coordination of transient competence with cell-cell killing, observed in multiple species, was found to result in synergistic blocking of MGE transmission through releasing genomic DNA for homologous recombination while simultaneously reducing horizontal MGE spread by lowering the local cell density. To evaluate the feasibility of the functions suggested by the modelling analysis, we analysed genomic data from longitudinal sampling of individuals carrying Streptococcus pneumoniae. This revealed the frequent within-host coexistence of clonally descended cells that differed in their MGE infection status, a necessary condition for the proposed mechanism to operate. Additionally, we found multiple examples of MGEs inhibiting transformation through integrative disruption of genes encoding the competence machinery across many species, providing evidence of an ongoing "arms race." Reduced rates of transformation have also been observed in cells infected by MGEs that reduce the concentration of extracellular DNA through secretion of DNases. Simulations predicted that either mechanism of limiting transformation would benefit individual MGEs, but also that this tactic's effectiveness was limited by competition with other MGEs coinfecting the same cell. A further observed behaviour we hypothesised to reduce elimination by transformation was MGE activation when cells become competent. Our model predicted that this response was effective at counteracting transformation independently of competing MGEs. Therefore, this framework is able to explain both common properties of MGEs, and the seemingly paradoxical bacterial behaviours of transformation and cell-cell killing within clonally related populations, as the consequences of intragenomic conflict between self-replicating chromosomes and parasitic MGEs. The antagonistic nature of the different mechanisms of HDT over short timescales means their contribution to bacterial evolution is likely to be substantially greater than previously appreciated
Genotypic and phenotypic heterogeneity in Streptococcus mutans isolated from diabetic patients in Rome, Italy
Inhibition of sialidase activity and cellular invasion by the bacterial vaginosis pathogen Gardnerella vaginalis
Bacterial vaginosis is a genital tract infection, thought to be caused by transformation of a lactobacillus-rich flora to a dysbiotic microbiota enriched in mixed anaerobes. The most prominent of these is Gardnerella vaginalis (GV), an anaerobic pathogen that produces sialidase enzyme to cleave terminal sialic acid residues from human glycans. Notably, high sialidase activity is associated with preterm birth and low birthweight. We explored the potential of the sialidase inhibitor Zanamavir against GV whole cell sialidase activity using methyl-umbelliferyl neuraminic acid (MU-NANA) cleavage assays, with Zanamavir causing a 30% reduction in whole cell GV sialidase activity (pâ<â0.05). Furthermore, cellular invasion assays using HeLa cervical epithelial cells, infected with GV, demonstrated that Zanamivir elicited a 50% reduction in cell association and invasion (pâ<â0.05). Our data thus highlight that pharmacological sialidase inhibitors are able to modify BV-associated sialidase activity and influence host-pathogen interactions and may represent novel therapeutic adjuncts
Increased Litterfall in Tropical Forests Boosts the Transfer of Soil CO2 to the Atmosphere
Aboveground litter production in forests is likely to increase as a consequence of elevated atmospheric carbon dioxide (CO2) concentrations, rising temperatures, and shifting rainfall patterns. As litterfall represents a major flux of carbon from vegetation to soil, changes in litter inputs are likely to have wide-reaching consequences for soil carbon dynamics. Such disturbances to the carbon balance may be particularly important in the tropics because tropical forests store almost 30% of the global soil carbon, making them a critical component of the global carbon cycle; nevertheless, the effects of increasing aboveground litter production on belowground carbon dynamics are poorly understood. We used long-term, large-scale monthly litter removal and addition treatments in a lowland tropical forest to assess the consequences of increased litterfall on belowground CO2 production. Over the second to the fifth year of treatments, litter addition increased soil respiration more than litter removal decreased it; soil respiration was on average 20% lower in the litter removal and 43% higher in the litter addition treatment compared to the controls but litter addition did not change microbial biomass. We predicted a 9% increase in soil respiration in the litter addition plots, based on the 20% decrease in the litter removal plots and an 11% reduction due to lower fine root biomass in the litter addition plots. The 43% measured increase in soil respiration was therefore 34% higher than predicted and it is possible that this âextraâ CO2 was a result of priming effects, i.e. stimulation of the decomposition of older soil organic matter by the addition of fresh organic matter. Our results show that increases in aboveground litter production as a result of global change have the potential to cause considerable losses of soil carbon to the atmosphere in tropical forests
Opsonising antibodies to P. falciparum Merozoites associated with immunity to clinical malaria
Naturally acquired humoral immunity to the malarial parasite Plasmodium falciparum can protect against disease, although the precise mechanisms remain unclear. Although antibody levels can be measured by ELISA, few studies have investigated functional antibody assays in relation to clinical outcomes. In this study we applied a recently developed functional assay of antibody-mediated opsonisation of merozoites, to plasma samples from a longitudinal cohort study conducted in a malaria endemic region of Papua New Guinea (PNG). Phagocytic activity was quantified by flow cytometry using a standardized and high-throughput protocol, and was subsequently evaluated for association with protection from clinical malaria and high-density parasitemia. Opsonising antibody responses were found to: i) increase with age, ii) be enhanced by concurrent infection, and iii) correlate with protection from clinical episodes and high-density parasitemia. Stronger protective associations were observed in individuals with no detectable parasitemia at baseline. This study presents the first evidence for merozoite phagocytosis as a correlate of acquired immunity and clinical protection against P. falciparum malaria
Beam-Target Double Spin Asymmetry A_LT in Charged Pion Production from Deep Inelastic Scattering on a Transversely Polarized He-3 Target at 1.4<Q^2<2.7 GeV^2
We report the first measurement of the double-spin asymmetry for
charged pion electroproduction in semi\nobreakdash-inclusive
deep\nobreakdash-inelastic electron scattering on a transversely polarized
He target. The kinematics focused on the valence quark region,
with . The corresponding neutron
asymmetries were extracted from the measured He asymmetries and
proton over He cross section ratios using the effective polarization
approximation. These new data probe the transverse momentum dependent parton
distribution function and therefore provide access to quark
spin-orbit correlations. Our results indicate a positive azimuthal asymmetry
for production on He and the neutron, while our
asymmetries are consistent with zero.Comment: 6 pages, 2 figures, 1 tables, published in PR
Single Spin Asymmetries in Charged Pion Production from Semi-Inclusive Deep Inelastic Scattering on a Transversely Polarized He Target
We report the first measurement of target single spin asymmetries in the
semi-inclusive reaction on a transversely polarized
target. The experiment, conducted at Jefferson Lab using a 5.9 GeV electron
beam, covers a range of 0.14 0.34 with 1.3 2.7 GeV. The
Collins and Sivers moments were extracted from the azimuthal angular dependence
of the measured asymmetries. The extracted Collins moments for He
are consistent with zero, except for the moment at , which
deviates from zero by 2.3. While the Sivers moments are
consistent with zero, the Sivers moments favor negative values. The
neutron results were extracted using the nucleon effective polarization and the
measured cross section ratio of proton to He, and are largely consistent
with the predictions of phenomenological fits and quark model calculations.Comment: 6 pages, 2 figures, 2 tables, published in PR
Seasonal drought limits tree species across the Neotropics
AcceptedArticle in Press© 2016 Nordic Society Oikos.Within the tropics, the species richness of tree communities is strongly and positively associated with precipitation. Previous research has suggested that this macroecological pattern is driven by the negative effect of water-stress on the physiological processes of most tree species. This implies that the range limits of taxa are defined by their ability to occur under dry conditions, and thus in terms of species distributions predicts a nested pattern of taxa distribution from wet to dry areas. However, this 'dry-tolerance' hypothesis has yet to be adequately tested at large spatial and taxonomic scales. Here, using a dataset of 531 inventory plots of closed canopy forest distributed across the western Neotropics we investigated how precipitation, evaluated both as mean annual precipitation and as the maximum climatological water deficit, influences the distribution of tropical tree species, genera and families. We find that the distributions of tree taxa are indeed nested along precipitation gradients in the western Neotropics. Taxa tolerant to seasonal drought are disproportionally widespread across the precipitation gradient, with most reaching even the wettest climates sampled; however, most taxa analysed are restricted to wet areas. Our results suggest that the 'dry tolerance' hypothesis has broad applicability in the world's most species-rich forests. In addition, the large number of species restricted to wetter conditions strongly indicates that an increased frequency of drought could severely threaten biodiversity in this region. Overall, this study establishes a baseline for exploring how tropical forest tree composition may change in response to current and future environmental changes in this region.This paper is a product of the RAINFOR and ATDN networks and of ForestPlots.net
researchers (http://www.forestplots.net). RAINFOR and ForestPlots have been
supported by a Gordon and Betty Moore Foundation grant, the European Unionâs
Seventh Framework Programme (283080, âGEOCARBONâ; 282664,
âAMAZALERTâ); European Research Council (ERC) grant âTropical Forests in the
Changing Earth Systemâ (T-FORCES), and Natural Environment Research Council
(NERC) Urgency Grant and NERC Consortium Grants âAMAZONICAâ
(NE/F005806/1) and âTROBITâ (NE/D005590/1). Additional funding for fieldwork was
provided by Tropical Ecology Assessment and Monitoring (TEAM) Network, a
collaboration among Conservation International, the Missouri Botanical Garden, the
Smithsonian Institution, and the Wildlife Conservation Society. A.E.M. receives a PhD
scholarship from the T-FORCES ERC grant. O.L.P. is supported by an ERC Advanced
Grant and a Royal Society Wolfson Research Merit Award. We thank Jon J. Lloyd,
Chronis Tzedakis, David Galbraith, and two anonymous reviewers for helpful
comments and Dylan Young for helping with the analyses. This study would not be
possible without the extensive contributions of numerous field assistants and rural
communities in the Neotropical forests. Alfredo AlarcĂłn, Patricia Alvarez Loayza,
PlĂnio Barbosa Camargo, Juan Carlos Licona, Alvaro Cogollo, Massiel Corrales
Medina, Jose Daniel Soto, Gloria Gutierrez, Nestor Jaramillo Jarama, Laura Jessica
Viscarra, Irina Mendoza Polo, Alexander Parada Gutierrez, Guido Pardo, Lourens
Poorter, Adriana Prieto, Freddy Ramirez Arevalo, AgustĂn Rudas, Rebeca Sibler and
Javier Silva Espejo additionally contributed data to this study though their RAINFOR
participations. We further thank those colleagues no longer with us, Jean Pierre Veillon,
Samuel Almeida, Sandra Patiño and Raimundo Saraiva. Many data come from Alwyn
Gentry, whose example has inspired new generations to investigate the diversity of the
Neotropics
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