Cell sorting in a Petri dish controlled by computer vision.

Fluorescence-activated cell sorting (FACS) applying flow cytometry to separate cells on a molecular basis is a widespread method. We demonstrate that both fluorescent and unlabeled live cells in a Petri dish observed with a microscope can be automatically recognized by computer vision and picked up by a computer-controlled micropipette. This method can be routinely applied as a FACS down to the single cell level with a very high selectivity. Sorting resolution, i.e., the minimum distance between two cells from which one could be selectively removed was 50-70 micrometers. Survival rate with a low number of 3T3 mouse fibroblasts and NE-4C neuroectodermal mouse stem cells was 66 +/- 12% and 88 +/- 16%, respectively. Purity of sorted cultures and rate of survival using NE-4C/NE-GFP-4C co-cultures were 95 +/- 2% and 62 +/- 7%, respectively. Hydrodynamic simulations confirmed the experimental sorting efficiency and a cell damage risk similar to that of normal FACS

Improved genome editing in human cell lines using the CRISPR method

The Cas9/CRISPR system has become a popular choice for genome editing. In this system, binding of a single guide (sg) RNA to a cognate genomic sequence enables the Cas9 nuclease to induce a double-strand break at that locus. This break is next repaired by an error-prone mechanism, leading to mutation and gene disruption. In this study we describe a range of refinements of the method, including stable cell lines expressing Cas9, and a PCR based protocol for the generation of the sgRNA. We also describe a simple methodology that allows both elimination of Cas9 from cells after gene disruption and re-introduction of the disrupted gene. This advance enables easy assessment of the off target effects associated with gene disruption, as well as phenotype-based structure-function analysis. In our study, we used the Fan1 DNA repair gene as control in these experiments. Cas9/CRISPR-mediated Fan1 disruption occurred at frequencies of around 29%, and resulted in the anticipated spectrum of genotoxin hypersensitivity, which was rescued by re-introduction of Fan1

The effect of umbilical cord blood derived mesenchymal stem cells in monocrotaline-induced pulmonary artery hypertension rats

Pulmonary arterial hypertension (PAH) causes right ventricular failure due to a gradual increase in pulmonary vascular resistance. The purposes of this study were to confirm the engraftment of human umbilical cord blood-mesenchymal stem cells (hUCB-MSCs) placed in the correct place in the lung and research on changes of hemodynamics, pulmonary pathology, immunomodulation and several gene expressions in monocrotaline (MCT)induced PAH rat models after hUCB-MSCs transfusion. The rats were grouped as follows: the control (C) group; the M group (MCT 60 mg/kg); the U group (hUCB-MSCs transfusion). They received transfusions via the external jugular vein a week after MCT injection. The mean right ventricular pressure (RVP) was significantly reduced in the U group after the 2 week. The indicators of RV hypertrophy were significantly reduced in the U group at week 4. Reduced medial wall thickness in the pulmonary arteriole was noted in the U group at week 4. Reduced number of intra-acinar muscular pulmonary arteries was observed in the U group after 2 week. Protein expressions such as endothelin (ET)-1, endothelin receptor A (ERA), endothelial nitric oxide synthase (eNOS) and matrix metalloproteinase (MMP)-2 significantly decreased at week 4. The decreased levels of ERA, eNOS and MMP-2 immunoreactivity were noted by immnohistochemical staining. After hUCB-MSCs were administered, there were the improvement of RVH and mean RVP. Reductions in several protein expressions and immunomodulation were also detected. It is suggested that hUCB-MSCs may be a promising therapeutic option for PAH.1174Ysciescopu

The Student Movement Volume 105 Issue 7: Putting the Finishing Touches on Fall Semester

NEWS Andrews Releases Spring 2021 Opening Plan, Amanda Cho Creating Visions for the Future, Joelle Kim Semester Reflections: Living During a Global Pandemic, Taylor Uphus PULSE The Best Drive-Through Meals, Masy Domecillo Reviewing Leaving the Shadowland of Stress, Depression, and Anxiety , Jessica Rim Study Tips: Re-Examining Routine, Wambui Karanja HUMANS Christmas vs. Thanksgiving, Interviewed by Abigail Lee Interview with Dongchan Kim, AUSA Executive Vice President, Interviewd by Ben Lee Studying Abroad in Argentina: An Interview with Lisiane Umuhire, Interviewed by TJ Hunter ARTS & ENTERTAINMENT Creative Spotlight: Ivan Rachath, Interviewed by Megan Napod Elementary, Hannah Cruse It\u27s Beginning to Sound A Lot Like Christmas!, Megan Napod IDEAS The Good, Kyara Samuels The Power of Resource, Evin N. Musgrove LAST WORD Fallen Titans: Remembering Alex Trebek and Rabbi Jonathan Sacks, Daniel Selfhttps://digitalcommons.andrews.edu/sm-105/1008/thumbnail.jp

Scapular deformity in obstetric brachial plexus palsy: a new finding

While most obstetric brachial plexus palsy patients recover arm and hand function, the residual nerve weakness leads to muscle imbalances about the shoulder which may cause bony deformities. In this paper we describe abnormalities in the developing scapula and the glenohumeral joint. We introduce a classification for the deformity which we term Scapular Hypoplasia, Elevation and Rotation. Multiple anatomic parameters were measured in bilateral CT images and three-dimensional CT reconstruction of the shoulder girdle of 30 obstetric brachial plexus palsy patients (age range 10 months–10.6 years). The affected scapulae were found to be hypoplastic by an average of 14% while the ratio of the height to the width of the body of scapula (excluding acromion) were not significantly changed, the acromion was significantly elongated by an average of 19%. These parameters as well as subluxation of the humeral head (average 14%) and downward rotation in the scapular plane were found to correlate with the area of scapula visible over the clavicle. This finding provides a classification tool for diagnosis and objective evaluation of the bony deformity and its severity in obstetric brachial plexus palsy patients

Microwave amplification with nanomechanical resonators

Sensitive measurement of electrical signals is at the heart of modern science and technology. According to quantum mechanics, any detector or amplifier is required to add a certain amount of noise to the signal, equaling at best the energy of quantum fluctuations. The quantum limit of added noise has nearly been reached with superconducting devices which take advantage of nonlinearities in Josephson junctions. Here, we introduce a new paradigm of amplification of microwave signals with the help of a mechanical oscillator. By relying on the radiation pressure force on a nanomechanical resonator, we provide an experimental demonstration and an analytical description of how the injection of microwaves induces coherent stimulated emission and signal amplification. This scheme, based on two linear oscillators, has the advantage of being conceptually and practically simpler than the Josephson junction devices, and, at the same time, has a high potential to reach quantum limited operation. With a measured signal amplification of 25 decibels and the addition of 20 quanta of noise, we anticipate near quantum-limited mechanical microwave amplification is feasible in various applications involving integrated electrical circuits.Comment: Main text + supplementary information. 14 pages, 3 figures (main text), 18 pages, 6 figures (supplementary information

The Student Movement Volume 105 Issue 2: Clubs Fare Well: Clubs Preserve Community During COVID

NEWS Clubs, Clubs, Clubs Galore, Taylor Uphus The Andreasen Center for Wellness Reopening, Amanda Cho Danielle Pilgrim Delivers Inspiring Message, Terika Williams PULSE Planning University Club Events Amidst a Pandemic, Masy Domecillo Research at the Science Complex During COVID-19, Jessica Rim Socializing During a Pandemic: Freshmen Perspectives, Interviewed by Wambui Karanja HUMANS BSCF Club Spotlight, Interviewed by TJ Hunter Distance Learning Student Interview, Sydney Saint-Jean Meet Lamson Hall\u27s Newest Dean, Interviewed by Pearl Parker Spiritual Leadership During COVID-19, Interviewed by Terika Williams ARTS & ENTERTAINMENT More Apples to Byte, Hannah Cruse Playing Out the Pandemic: Our Orchestras Dealing with COVID, Megan Napod Signal Boost: The Domino Effect, Pearl Parker Virtual Game Night: A Virtual Reality, Joshua Deonarine IDEAS How Climate Change is Fueling the West Coast Fires, Lyle Goulbourne Ideas That Heal Us: So You Think You\u27re Self-Aware, Evin N. Musgrove THE LAST WORD What am I Applying for & Who am I?, Daniel Selfhttps://digitalcommons.andrews.edu/sm-105/1002/thumbnail.jp

The Student Movement Volume 105 Issue 4: Students Destress in AUSA\u27s Nest

NEWS AU Adelante Club Hosts Vespers, Joelle Kim AUSA Hosts The Nest, Amanda Cho The Gazebo Reopens with GetFood App, Taylor Uphus PULSE Cultural Hispanic Catchphrases, Wambui Karanja Keep Calm and Breathing On (Yourself), Jessica Rim Meet & Make: Reflections, Masy Domecillo HUMANS Event Planning With Malachi Regis, Interviewed by Fitz-Earl McKenzie II Interview with Michael Nixon: Vice President for Diversity & Inclusion, Interviewed by Abigail Lee Meet Professor Pedro Navia, Interviewed by Pearl Parker Torian Hill, Interviewed by TJ Hunter ARTS & ENTERTAINMENT Hispanic Artist Feature: Felix Gillett, Megan Napod Música para el Alma or Music for the Soul, Hannah Cruse Signal Boost, Alannah Tjhatra IDEAS An Defense Against Burnout: Why Meaning Matters, Adoniah Simon Remembering RBG: Part Two, Lyle Goulbourne THE LAST WORD How Does it Change Us?, Daniel Selfhttps://digitalcommons.andrews.edu/sm-105/1004/thumbnail.jp

Attention deficit hyperactivity symptoms predict problematic mobile phone use

Attention-deficit-hyperactivity disorder (ADHD) is the most commonly diagnosed childhood disorder characterised by inattention, hyperactivity/impulsivity, or both. Some of the key traits of ADHD have previously been linked to addictive and problematic behaviours. The aim of the present study was to examine the relationship between problematic mobile phone use, smartphone addiction risk and ADHD symptoms in an adult population. A sample of 273 healthy adult volunteers completed the Adult ADHD Self-Report Scale (ASRS), the Mobile Phone Problem Usage Scale (MPPUS), and the Smartphone Addiction Scale (SAS). A significant positive correlation was found between the ASRS and both scales. More specifically, inattention symptoms and age predicted smartphone addiction risk and problematic mobile phone use. Our results suggest that there is a positive relationship between ADHD traits and problematic mobile phone use. In particular, younger adults with higher level of inattention symptoms could be at higher risk of developing smartphone addiction. The implication of our findings for theoretical frameworks of problematic mobile phone use and clinical practice are discussed

Biological effects of rinsing morsellised bone grafts before and after impaction

Rinsing bone grafts before or both before and after impaction might have different effects on the incorporation of the graft. Rinsing again after impaction might negatively influence bone induction if growth factors released by impaction are washed away. We studied if transforming growth factor-βs (TGF-βs) and bone morphogenetic proteins (BMPs) are released from the mineralised matrix by impaction and if these released growth factors induce osteogenic differentiation in human mesenchymal stem cells (hMSCs). Rinsed morsellised bone allografts were impacted in a cylinder and the escaping fluid was collected. The fluid was analysed for the presence of TGF-βs and BMPs, and the osteoinductive capacity was tested on hMSCs. Abundant TGF-β was present in the fluid. No BMPs could be detected. Osteogenic differentiation of hMSCs was inhibited by the fluid. Results from our study leave us only able to speculate whether rinsing grafts again after impaction has a beneficial effect on the incorporation process or not