Time Reversal Aided Bidirectional OFDM Underwater Cooperative Communication Algorithm with the Same Frequency Transmission

In underwater acoustic channel, signal transmission may experience significant latency and attenuation that would degrade the performance of underwater communication. The cooperative communication technique can solve it but the spectrum efficiency is lower than traditional underwater communication. So we proposed a time reversal aided bidirectional OFDM underwater cooperative communication algorithm. The algorithm allows all underwater sensor nodes to share the same uplink and downlink frequency simultaneously to improve the spectrum efficiency. Since the same frequency transmission would produce larger intersymbol interference, we adopted the time reversal method to degrade the multipath interference at first; then we utilized the self-information cancelation module to remove the self-signal of OFDM block because it is known for sensor nodes. In the simulation part, we compare our proposed algorithm with the existing underwater cooperative transmission algorithms in respect of bit error ratio, transmission rate, and computation. The results show that our proposed algorithm has double spectrum efficiency under the same bit error ratio and has the higher transmission rate than the other underwater communication methods

Height Information Aided 3D Real-Time Large-Scale Underground User Positioning

Due to the cost of inertial navigation and visual navigation equipment and lake of satellite navigation signals, they cannot be used in large‐scale underground mining environment. To solve this problem, this study proposes large‐scale underground 3D real‐time positioning method with seam height assistance. This method uses the ultrawide band positioning base station as the core and is combined with seam height information to build a factor graph confidence transfer model to realise3D positioning. The simulation results show that the proposed real‐time method is superior to the existing algorithms in positioning accuracy and can meet the needs of large‐scale underground users

A Survey on the applications of IoT: an investigation into existing environments, present challenges and future opportunities

In today’s digital environment, devices are able to interconnect and react to contextual data more than ever before: artificial intelligence is beginning to coordinate how data collected from sensors and de-vices within the network is analysed, and device ecosystems are replacing standalone devices to deliver solutions to the user. In this paper, the researcher explores current implementations of IoT that have led to positive outcomes for the user; but also, the challenges that remain in today’s applications. Moreover, ex-ploring these current barriers may be able to infer future applications capable of being deployed on a global scal

MEK Guards Proteome Stability and Inhibits Tumor-Suppressive Amyloidogenesis via HSF1

SummarySignaling through RAS/MAP kinase pathway is central to biology. ERK has long been perceived as the only substrate for MEK. Here, we report that HSF1, the master regulator of the proteotoxic stress response, is a new MEK substrate. Beyond mediating cell-environment interactions, the MEK-HSF1 regulation impacts malignancy. In tumor cells, MEK blockade inactivates HSF1 and thereby provokes proteomic chaos, presented as protein destabilization, aggregation, and, strikingly, amyloidogenesis. Unlike their non-transformed counterparts, tumor cells are particularly susceptible to proteomic perturbation and amyloid induction. Amyloidogenesis is tumor suppressive, reducing in vivo melanoma growth and contributing to the potent anti-neoplastic effects of proteotoxic stressors. Our findings unveil a key biological function of the oncogenic RAS-MEK signaling in guarding proteostasis and suppressing amyloidogenesis. Thus, proteomic instability is an intrinsic feature of malignant state, and disrupting the fragile tumor proteostasis to promote amyloidogenesis may be a feasible therapeutic strategy

Loss of tumor suppressor NF1 activates HSF1 to promote carcinogenesis

Intrinsic stress response pathways are frequently mobilized within tumor cells. The mediators of these adaptive mechanisms and how they contribute to carcinogenesis remain poorly understood. A striking example is heat shock factor 1 (HSF1), master transcriptional regulator of the heat shock response. Surprisingly, we found that loss of the tumor suppressor gene neurofibromatosis type 1 (Nf1) increased HSF1 levels and triggered its activation in mouse embryonic fibroblasts. As a consequence, Nf1[superscript –/–] cells acquired tolerance to proteotoxic stress. This activation of HSF1 depended on dysregulated MAPK signaling. HSF1, in turn, supported MAPK signaling. In mice, Hsf1 deficiency impeded NF1-associated carcinogenesis by attenuating oncogenic RAS/MAPK signaling. In cell lines from human malignant peripheral nerve sheath tumors (MPNSTs) driven by NF1 loss, HSF1 was overexpressed and activated, which was required for tumor cell viability. In surgical resections of human MPNSTs, HSF1 was overexpressed, translocated to the nucleus, and phosphorylated. These findings reveal a surprising biological consequence of NF1 deficiency: activation of HSF1 and ensuing addiction to this master regulator of the heat shock response. The loss of NF1 function engages an evolutionarily conserved cellular survival mechanism that ultimately impairs survival of the whole organism by facilitating carcinogenesis.United States. Army Medical Research and Materiel Command (Neurofibromatosis Research Program)Kathy and Curt Marble Cancer Research Fun

Overexpression of a MYB Family Gene, OsMYB6, Increases Drought and Salinity Stress Tolerance in Transgenic Rice

MYB transcription factors have been demonstrated to play key regulatory roles in plant growth, development and abiotic stress response. However, knowledge concerning the involvement of rice MYB genes in salinity and drought stress resistance are largely unknown. In the present study, we cloned and characterized the OsMYB6 gene, which was induced by drought and salinity stress. Subcellular localization of OsMYB6-YFP fusion protein in protoplast cells indicated that OsMYB6 was localized in the nucleus. Overexpression of OsMYB6 in rice did not suggest a negative effect on the growth and development of transgenic plants, but OsMYB6-overexpressing plants showed increased tolerance to drought and salt stress compared with wild-type plants, as are evaluated by higher proline content, higher CAT and SOD activities, lower REL and MDA content in transgenic plants under drought and salt stress conditions. In addition, the expression of abiotic stress-responsive genes were significantly higher in OsMYB6 transgenic plants than that in wild-type plants under drought and salt stress conditions. These results indicate that OsMYB6 gene functions as a stress-responsive transcription factor which plays a positive regulatory role in response to drought and salt stress resistance, and may be used as a candidate gene for molecular breeding of salt-tolerant and drought-tolerant crop varieties

Effect of mechanical stimulation on tissue heterotopic ossification: an in vivo experimental study

Background: Heterotopic ossification of tendons and ligaments (HOTL) is a common clinical condition characterized by the absence of discernible features and a lack of effective treatment. In vitro experiments have demonstrated that mechanical stimulation can induce cell differentiation toward osteogenesis, thereby promoting heterotopic ossification. Currently, there are few experimental designs aimed at inducing ligament stretching in mice, and the mechanism of heterotopic ossification may not entirely mirror that observed in clinical cases. Therefore, there is an urgent imperative to develop a novel and feasible animal model.Methods: In this study, all the Enpp1 gene deficiency mice (a mouse model with heterotopic ossification of multiple ligaments) were divided into three groups: the control group, the spinal brake group, and the hyperactive group (treadmill training group). An external spinal fixation device was designed to restrict mice’s spinal flexion and extension at 6 weeks of age. The brace was adjusted weekly according to the changes in the size of the mice. Additionally, treadmill training was used to increase activity in the spinal ligaments and Achilles tendons of the mice. Micro-CT scanning and HE staining were performed at 12, 20, and 28 W to evaluate the degree of ossification in the spinal ligament and Achilles tendon. What’s more, As one of the mechanical stimulation transduction signals, YAP plays a crucial role in promoting osteogenic differentiation of cells. Immunofluorescence was utilized to assess YAP expression levels for the purpose of determining the extent of mechanical stimulation in tissues.Results: Our findings showed that a few ossification lesions were detected behind the vertebral space of mice at 8 weeks of age. Spinal immobilization effectively restricts the flexion and extension of cervical and thoracic vertebrae in mice, delaying spinal ligament ossification and reducing chronic secondary spinal cord injury. Running exercises not only enhance the ossification area of the posterior longitudinal ligament (PLL) and Achilles tendons but also exacerbate secondary spinal cord injury. Further immunofluorescence results revealed a notable increase in YAP expression levels in tissues with severe ossification, suggesting that these tissues may be subjected to higher mechanical stimulation.Conclusion: Mechanical stimulation plays a pivotal role in the process of heterotopic ossification in tissues. Our study provided valid animal models to further explore the pathological mechanism of mechanical stimulation in HOTL development

Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA).

RAS/MAPK signaling responds to diverse extracellular cues and regulates a wide array of cellular processes. Given its biological importance, abnormalities in RAS/MAPK signaling cascade have been intimately implicated in numerous human diseases, including cancer. Herein, we describe a novel methodology to study activation of this pivotal signaling pathway. The Proximity Ligation Assay (PLA) is employed to monitor kinase-substrate interactions between MEK1 and HSF1, or MEK1 and ERK1 in situ. Methods Mol Biol 2017; 1487:195-201

Robust Multipath-Assisted SLAM with Unknown Process Noise and Clutter Intensity

In multipath-assisted simultaneous localization and mapping (SLAM), the geometric association of specular multipath components based on radio signals with environmental features is used to simultaneously localize user equipment and map the environment. We must contend with two notable model parameter uncertainties in multipath-assisted SLAM: process noise and clutter intensity. Knowledge of these two parameters is critically important to multipath-assisted SLAM, the uncertainty of which will seriously affect the SLAM accuracy. Conventional multipath-assisted SLAM algorithms generally regard these model parameters as fixed and known, which cannot meet the challenges presented in complicated environments. We address this challenge by improving the belief propagation (BP)-based SLAM algorithm and proposing a robust multipath-assisted SLAM algorithm that can accommodate model mismatch in process noise and clutter intensity. Specifically, we describe the evolution of the process noise variance and clutter intensity via Markov chain models and integrate them into the factor graph representing the Bayesian model of the multipath-assisted SLAM. Then, the BP message passing algorithm is leveraged to calculate the marginal posterior distributions of the user equipment, environmental features and unknown model parameters to achieve the goals of simultaneous localization and mapping, as well as adaptively learning the process noise variance and clutter intensity. Finally, the simulation results demonstrate that the proposed approach is robust against the uncertainty of the process noise and clutter intensity and shows excellent performances in challenging indoor environments