SKYWAY '09: Awareness of the Sky/ The Sky as Awareness - International Light Festival, Impressions and Reflections on the Proceedings

Two artists, two astrophysicists, a historian of science, a musician, the Skyway Festival director and the Toruń 2016 director met over two days on the 15th and 16th of August in this beautiful gothic city to discuss the relationship between art and astrophysics. The aim of the sessions was to foster interdisciplinary understanding and achieve a consensus on methods and approaches for future collaborative work and cross-disciplinary production of art, writing and dissemination.Peer reviewe

Sustainability assessment in higher education institutions

UID/AMB/04085/2019 UIDP/50017/2020 UIDB/50017/2020This Special Issue "Sustainability Assessment in Higher Education Institutions" provides peer-reviewed research from several geographies and institutions and covering various topics with the broad objective of achieving an assessment of the effectiveness and impact of different implementation dimensions measuring and evaluating how sustainability is being applied in practice. A set of nine papers, covering sustainability education, interdisciplinary teaching, sustainable assessment, governance strategies, commitments and practices, and social responsibility at Higher Education Institutions, contribute significantly to this area of knowledge.publishersversionpublishe

Biodiversity and characterization of marine mycota from Portuguese waters

Biodiversity and characterization of marine mycota from Portuguese waters.— The occurrence, diversity and similarity of marine fungi detected by the sum of direct and indirect observations in Fagus sylvatica and Pinus pinaster baits submerged at two Portuguese marinas are analyzed and discussed. In comparison with the data already published in 2010, the higher number of specimens considered in this study led to the higher number of very frequent taxa for these environments and substrata; the significant difference in substrata and also in fungal diversity detected at the two environments is also highlighted, in addition to the decrease in fungal similarity. Because the identification of Lulworthia spp., Fusarium sp., Graphium sp., Phoma sp. and Stachybotrys sp. down to species level was not possible, based only on the morphological characterization, a molecular approach based on the amplification of the LSU rDNA region was performed with isolates of these fungi. This was achieved for three isolates, identified as Fusarium solani, Graphium eumorphum and Stachybotrys chartarum. To achieve this with the other isolates which are more complex taxa, the sequencing of more regions will be considered.Biodiversidad y caracterización de los hongos marinos de las aguas portuguesas.— Se analiza y discute la presencia, la diversidad y la similitud de los hongos marinos detectados mediante la suma de observaciones directas e indirectas utilizando cebos de Fagus sylvatica y Pinus pinaster sumergidos en dos puertos deportivos portugueses. En comparación con los datos ya publicados en 2010, el mayor número de especímenes aquí considerados condujo a un mayor número de taxones muy frecuentes en estos sustratos y medios ambientales; también debe destacarse la diferencia significativa en los sustratos y también en la diversidad fúngica en los dos medios ambientales, además de la disminución de la similitud fúngica. Dado que no fue posible la identificación de Lulworthia spp., Fusarium sp., Graphium sp., Phoma sp., y Stachybotrys sp. hasta el nivel de especie, basándose únicamente en la caracterización morfológica, se llevó a cabo un estudio molecular basado en la amplificación de la región LSU ADNr con extractos de dichos hongos. Ello se consiguió en tres extractos puros, identificados como de Fusarium solani, Graphium eumorphum y Stachybotrys chartarum. Para llevar a cabo este proceso con otros extractos puros pertenecientes a taxones más complejos, se considerará la secuenciación de más regiones.publishe

Multi-organ histological observations on juvenile senegalese soles exposed to low concentrations of waterborne cadmium

A histopathological screening was performed on juvenile Senegalese soles exposed to environmentally realistic concentrations of waterborne Cd (0.5, 5 and 10 lg L-1 ) for 28 days. The severity and dissemination of histopathological changes were variable and limited to the kidney, liver, spleen, gills and skin goblet cells. Contradicting available literature that refers the liver as the most affected organ upon acute exposure and the kidney following chronic exposure, the liver was the most impacted organ (even at the lowest concentration), in a trend that could relate to the duration of exposure and Cd concentration. The most noticeable hepatic alterations related to inflammation, although hepatocellular alterations like lipidosis and eosinophilic foci also occurred. The trunk kidney of exposed fish endured moderate inflammation, apoptosis and necrosis, however, without a clear time-dependent effect. The spleen of fish subjected to the highest concentrations revealed diffuse necrotic foci accompanied by melanomacrophage intrusion. The gills, albeit the most important apical uptake organ of dissolved toxicants, sustained only moderate damage, from epithelial hyperplasia and pavement cell detachment to the potentially more severe chloride cell alterations. In the skin, an increase in goblet cell size occurred, most notoriously correlated to Cd concentration at earlier stages of exposure. The results show that a metal-naı¨ve juvenile fish can endure deleterious effects when exposed to low, ecologically relevant, concentrations of a common toxic metal and that the pattern of Cdinduced histopathological alterations can be complex and linked to organ-specific responses and metal translocation within the organism.info:eu-repo/semantics/publishedVersio

Detection and Quantification of Grass and Olea Airborne Pollen Allergens in Outdoor Air Samples and its Correlation with Pollen Counts

Detection and Quantification of Grass and Olea Airborne Pollen Allergens in Outdoor Air Samples and its Correlation with Pollen Counts R Ferro1*, R Ribeiro1*, MR Martins1,2, AT Caldeira1,3, E Caeiro6, CM Antunes1,5 & R Brandão2,4 and the HIALINE working group7 1Dep. of Chemistry, University of Evora, Portugal; 2Mediterranean Inst. Crop and Environment Sciences, Univ.Evora, Portugal; 3Centro Química, University of Évora, Portugal; 4Dep. Biology, University of Evora; 5Center for NeuroSciences and Cell Biology-University of Coimbra, Portugal; 6Soc.Portuguesa Alergol.Imunologia Clínica , Portugal 7 M. Thibaudon, France, M. Smith, United Kingdom, C. Galan, Spain R. Albertini, Italy, L. Grewling, Poland, G. Reese, Germany, A. Rantio-Lehtimäki, Finland, S. Jäger and U. Berger, Austria, M. Sofiev, Finland, I. Sauliene, Lithuania, L. Cecchi, Italy Presenting author: [email protected] tel: +351 266760889 Introduction: Allergic respiratory diseases broken out after an exposure to airborne pollen, as asthma and allergic rhinitis, are deeply increasing and they represent one of the major public health problems nowadays, affecting about 40% of European population. In Portugal, grass and Olea europaea pollen are certainly one of the main sources of athmospheric aeroallergens and as such, one of the main causes of respiratory allergy. For these reasons, it is useful the development of new strategies for prevention and treatment of these pathologies. The execution of aerobiological analysis including pollen calendars and/or immunoassays for the detection and quantification of allergens which could forecast the allergenic potential of the athmosphere are quite relevant since they would contribute to develop prevention measures of allergic respiratory diseases. The aim of this study was to evaluate the putative correlation between the concentration of some of the major allergens of and with their pollen counts. Methodology: On a meteorological platform at the town center of Evora (south Portugal), ambient air was sampled at 800L/min with a Chemvol high-volume cascade impactor equipped with stages PM>10µm, 10 µm>PM>2.5µm. The polyurethane impacting substrate was extracted with 0.1M NH4HCO3, pH8.1, supplemented with 0.1% BSA. The major pollen allergens from grass Phleum p 5 and olive Ole e 1 were determined with allergen specific ELISA´s. Airborne pollen of and Olea europaea simultaneously monitored with a Burkard Seven Day Recording Volumetric Spore Trap* , between the 30th of April and the 8th of July of 2009. Both samplers were placed side-by-side with air input at the same level. Results: During the pollen season of 2009, high values of grass pollen were recorded between May 2th and June 1 th. It was also observed that the air content of Phl p5 or Ole e1 aeroallergens were directly correlated with airborne pollen counts of Poaceae and Oleaceae, respectively. Conclusions: These results suggest that the directly quantification of aeroallergens may contribute, together with pollen counts of air samples, to define the allergic risk with higher precision. Acknowledgments: This study is integrated in the european project HIALINE (Executive Agency for Health and Consumers under grant agreement No 2008 11 07

The Use of Generalized Means in the Estimation of the Weibull Tail Coefficient

Due to the specificity of the Weibull tail coefficient, most of the estimators available in the literature are based on the log excesses and are consequently quite similar to the estimators used for the estimation of a positive extreme value index. The interesting performance of estimators based on generalized means leads us to base the estimation of the Weibull tail coefficient on the power mean-of-order-. Consistency and asymptotic normality of the estimators under study are put forward. Their performance for finite samples is illustrated through a Monte Carlo simulation. It is always possible to find a negative value of (contrarily to what happens with the mean-of-order- estimator for the extreme value index), such that, for adequate values of the threshold, there is a reduction in both bias and root mean square error

TEMPORAL AND SPATIAL DISTRIBUTION OF POACEAE POLLEN IN AREAS OF SOUTHERN UNITED KINGDOM, SPAIN AND PORTUGAL

Overall, longer Poaceae pollen seasons coincided with earlier pollen season start dates. Winter rainfall noticeably affects the intensity of Poaceae pollen seasons in Mediterranean areas, but this was not as important in Worcester. Weekly data from Worcester followed a similar pattern to that of Badajoz and Évora but at a distance of more than 1500 km and 4-5 weeks later

An integrative assessment to determine the genotoxic hazard of estuarine sediments: combining cell and whole-organism responses

The application of the Comet assay in environmental monitoring remains challenging in face of the complexity of environmental stressors, e.g., when dealing with estuarine sediments, that hampers the drawing of cause-effect relationships. Although the in vitro Comet assay may circumvent confounding factors, its application in environmental risk assessment (ERA) still needs validation. As such, the present work aims at integrating genotoxicity and oxidative DNA damage induced by sediment-bound toxicants in HepG2 cells with oxidative stress-related effects observed in three species collected from an impacted estuary. Distinct patterns were observed in cells exposed to crude mixtures of sediment contaminants from the urban/industrial area comparatively to the ones from the rural/riverine area of the estuary, with respect to oxidative DNA damage and oxidative DNA damage. The extracts obtained with the most polar solvent and the crude extracts caused the most significant oxidative DNA damage in HepG2 cells, as measured by the formamidopyrimidine-DNA glycosylase (FPG)-modified Comet assay. This observation suggests that metals and unknown toxicants more hydrophilic than polycyclic aromatic hydrocarbons may be important causative agents, especially in samples from the rural part of the estuary, where oxidative DNA damage was the most significant. Clams, sole, and cuttlefish responded differentially to environmental agents triggering oxidative stress, albeit yielding results accordant with the oxidative DNA damage observed in HepG2 cells. Overall, the integration of in vivo biomarker responses and Comet assay data in HepG2 cells yielded a comparable pattern, indicating that the in vitro FPG-modified Comet assay may be an effective and complementary line-of-evidence in ERA even in particularly challenging, natural, scenarios such as estuarine environments

Assessment of the presence of Hepatitis E virus in surface water and drinking water in Portugal

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Hepatitis E virus (HEV) is a non-enveloped single-stranded positive-sense RNA virus, belonging to the Hepeviridae family, resistant to environmental conditions, and transmitted by the consumption of contaminated water. This virus is responsible for both sporadic and epidemic outbreaks, leading to thousands of infections per year in several countries, and is thus considered an emerging disease in Europe and Asia. This study refers to a survey in Portugal during 2019, targeting the detection and eventual quantification of enteric viruses in samples from surface and drinking water. Samples positive for HEV RNA were recurrently found by reverse transcription quantitative PCR (RT-qPCR), in both types of matrix. The infectivity of these samples was evaluated in cultured Vero E6 cells and RNA from putative viruses produced in cultures evidencing cytopathic effects and was subjected to RT-qPCR targeting HEV genomic RNA. Our results evidenced the existence of samples positive either for HEV RNA (77.8% in surface water and 66.7% in drinking water) or for infectious HEV (23.0% in surface water and 27.7% in drinking water). These results highlight the need for effective virological control of water for human consumption and activities.This research was funded by Empresa Portuguesa das Águas Livres (EPAL) and Foundation for Science and Technology (FCT) Portugal through a Ph.D. grant to D. Salvador (PDE/BDE/114582/2016) and FCT/MCTES projects UIDP/50017/2020+UIDB/50017/2020 and UIDB/04295/2020 through national funds.info:eu-repo/semantics/publishedVersio

Minimum-variance reduced-bias estimation of the extreme value index: A theoretical and empirical study

In extreme value (EV) analysis, the EV index (EVI), , is the primary parame- ter of extreme events. In this work, we consider positive, that is, we assume that F is heavy tailed. Classical tail parameters estimators, such as the Hill, the Moments, or the Weissman estimators, are usually asymptotically biased. Con- sequently, those estimators are quite sensitive to the number of upper order statistics used in the estimation. Minimum-variance reduced-bias (RB) estima- tors have enabled us to remove the dominant component of asymptotic bias without increasing the asymptotic variance of the new estimators. The purpose of this paper is to study a new minimum-variance RB estimator of the EVI. Under adequate conditions, we prove their nondegenerate asymptotic behavior. More- over, an asymptotic and empirical comparison with other minimum-variance RB estimators from the literature is also provided. Our results show that the proposed new estimator has the potential to be very useful in practice