COMBINATION VACCINES AGAINST KOIHERPES VIRUS AND Aeromonas hydrophila CO-INFECTION IN KOI AND COMMON CARP

Co-infections occur when hosts are infected by two or more pathogens, either simultaneous or as a secondary infection. This research aimed to determine the best compositions of vaccine combinations and their protective efficacies against pathogens co-infection. This research was conducted in two stages. Firstly, surveys were conducted in three research areas: infected, high risk of infection, and virus free areas. Samples (three to five fish per pool) were collected from three fish farms per area. The basic antibody titer of fish from each farm was checked before and after vaccination as well as after the virus challenge in combination with the PCR result. The second stage of the research was conducted in the laboratory. Carp and koi fish were used to determine optimal vaccine combination and dosage for oral application. The results of this research showed that combination of KHV: Aeromonas hydrophila vaccines in the ratio of 1:2 and vaccine volume of 3 mL via the oral application gave higher titer antibody and efficacy against KHV and A. hydrophila. In conclusion, the combined vaccine offers an effective means of preventing the diseases, decreasing fish mortality, and simplifying the immunization schedule, which will eventually increase the overall health of farmed fish and benefit fish farmers and service extension officers. This research recommends that further development of the combined vaccines should be carried out, for example, overcoming the technical difficulties in its manufacturing

Genomic and biologic comparisons of cyprinid herpesvirus 3 strains

Cyprinid herpesvirus 3 (CyHV-3) is the archetypal fish alloherpesvirus and the etiologic agent of a lethal disease in common and koi carp. To date, the genome sequences of only four CyHV-3 isolates have been published, but no comparisons of the biologic properties of these strains have been reported. We have sequenced the genomes of a further seven strains from various geographical sources, and have compared their growth in vitro and virulence in vivo. The major findings were: (i) the existence of the two genetic lineages previously described as European and Asian was confirmed, but inconsistencies between the geographic origin and genotype of some strains were revealed; (ii) potential inter-lineage recombination was detected in one strain, which also suggested the existence of a third, as yet unidentified lineage; (iii) analysis of genetic disruptions led to the identification of non-essential genes and their potential role in virulence; (iv) comparison of the in vitro and in vivo properties of strains belonging to the two lineages revealed that inter-lineage polymorphisms do not contribute to the differences in viral fitness observed; and (v) a negative correlation was observed among strains between viral growth in vitro and virulence in vivo. This study illustrates the importance of coupling genomic and biologic comparisons of viral strains in order to enhance understanding of viral evolution and pathogenesis

Lepton number violating interactions and their effects on neutrino oscillation experiments

Mixing between bosons that transform differently under the standard model gauge group, but identically under its unbroken subgroup, can induce interactions that violate the total lepton number. We discuss four-fermion operators that mediate lepton number violating neutrino interactions both in a model-independent framework and within supersymmetry (SUSY) without R-parity. The effective couplings of such operators are constrained by: i) the upper bounds on the relevant elementary couplings between the bosons and the fermions, ii) by the limit on universality violation in pion decays, iii) by the data on neutrinoless double beta decay and, iv) by loop-induced neutrino masses. We find that the present bounds imply that lepton number violating neutrino interactions are not relevant for the solar and atmospheric neutrino problems. Within SUSY without R-parity also the LSND anomaly cannot be explained by such interactions, but one cannot rule out an effect model-independently. Possible consequences for future terrestrial neutrino oscillation experiments and for neutrinos from a supernova are discussed.Comment: 28 pages, 2 figures, Late

The evolving doublecortin (DCX) superfamily

BACKGROUND: Doublecortin (DCX) domains serve as protein-interaction platforms. Mutations in members of this protein superfamily are linked to several genetic diseases. Mutations in the human DCX gene result in abnormal neuronal migration, epilepsy, and mental retardation; mutations in RP1 are associated with a form of inherited blindness, and DCDC2 has been associated with dyslectic reading disabilities. RESULTS: The DCX-repeat gene family is composed of eleven paralogs in human and in mouse. Its evolution was followed across vertebrates, invertebrates, and was traced to unicellular organisms, thus enabling following evolutionary additions and losses of genes or domains. The N-terminal and C-terminal DCX domains have undergone sub-specialization and divergence. Developmental in situ hybridization data for nine genes was generated. In addition, a novel co-expression analysis for most human and mouse DCX superfamily-genes was performed using high-throughput expression data extracted from Unigene. We performed an in-depth study of a complete gene superfamily using several complimentary methods. CONCLUSION: This study reveals the existence and conservation of multiple members of the DCX superfamily in different species. Sequence analysis combined with expression analysis is likely to be a useful tool to predict correlations between human disease and mouse models. The sub-specialization of some members due to restricted expression patterns and sequence divergence may explain the successful addition of genes to this family throughout evolution

SBML Level 3 package: Render, Version 1, Release 1

Many software tools provide facilities for depicting reaction network diagrams in a visual form. Two aspects of such a visual diagram can be distinguished: the layout (i.e.: the positioning and connections) of the elements in the diagram, and the graphical form of the elements (for example, the glyphs used for symbols, the properties of the lines connecting them, and so on). This document describes the SBML Level 3 Render package that complements the SBML Level 3 Layout package and provides a means of capturing the precise rendering of the elements in a diagram. The SBML Level 3 Render package provides a flexible approach to rendering that is independent of both the underlying SBML model and the Layout information. There can be one block of render information that applies to all layouts or an additional block for each layout. Many of the elements used in the current render specification are based on corresponding elements from the SVG specification. This allows us to easily convert a combination of layout information and render information into a SVG drawing

A new age in AquaMedicine: unconventional approach in studying aquatic diseases

Background Marine and aquaculture industries are important sectors of the food production and global trade. Unfortunately, the fish food industry is challenged with a plethora of infectious pathogens. The freshwater and marine fish communities are rapidly incorporating novel and most up to date techniques for detection, characterization and treatment strategies. Rapid detection of infectious diseases is important in preventing large disease outbreaks. Main text One hundred forty-six articles including reviews papers were analyzed and their conclusions evaluated in the present paper. This allowed us to describe the most recent development research regarding the control of diseases in the aquatic environment as well as promising avenues that may result in beneficial developments. For the characterization of diseases, traditional sequencing and histological based methods have been augmented with transcriptional and proteomic studies. Recent studies have demonstrated that transcriptional based approaches using qPCR are often synergistic to expression based studies that rely on proteomic-based techniques to better understand pathogen-host interactions. Preventative therapies that rely on prophylactics such as vaccination with protein antigens or attenuated viruses are not always feasible and therefore, the development of therapies based on small nucleotide based medicine is on the horizon. Of those, RNAi or CRISPR/Cas- based therapies show great promise in combating various types of diseases caused by viral and parasitic agents that effect aquatic and fish medicine. Conclusions In our modern times, when the marine industry has become so vital for feed and economic stability, even the most extreme alternative treatment strategies such as the use of small molecules or even the use of disease to control invasive species populations should be considered

Can lepton flavor violating interactions explain the LSND results?

If the atmospheric and the solar neutrino problem are both explained by neutrino oscillations, and if there are only three light neutrinos, then all mass-squared differences between the neutrinos are known. In such a case, existing terrestrial neutrino oscillation experiments cannot be significantly affected by neutrino oscillations, but, in principle there could be an anomaly in the neutrino flux due to new neutrino interactions. We discuss how a non-standard muon decay $\mu^+ \to e^+ \bar\nu_e \nu_\ell$ would modify the neutrino production processes of these experiments. Since $SU(2)_L$ violation is small for New Physics above the weak scale one can use related flavor-violating charged lepton processes to constrain these decays in a model independent way. We show that the upper bounds on $\mu \to 3e$, muonium-antimuonium conversion and $\tau \to \mu e e$ rule out any observable effect for the present experiments due to $\mu^+ \to e^+ \bar\nu_e \nu_\ell$ for $\ell=e,\mu,\tau$, respectively. Applying similar arguments to flavor-changing semi-leptonic reactions we exclude the possibility that the "oscillation signals" observed at LSND are due to flavor-changing interactions that conserve total lepton number.Comment: 21 pages, 6 figures, Latex; minor correction

Plasma membrane H⁺ -ATPase regulation is required for auxin gradient formation preceding phototropic growth.

Phototropism is a growth response allowing plants to align their photosynthetic organs toward incoming light and thereby to optimize photosynthetic activity. Formation of a lateral gradient of the phytohormone auxin is a key step to trigger asymmetric growth of the shoot leading to phototropic reorientation. To identify important regulators of auxin gradient formation, we developed an auxin flux model that enabled us to test in silico the impact of different morphological and biophysical parameters on gradient formation, including the contribution of the extracellular space (cell wall) or apoplast. Our model indicates that cell size, cell distributions, and apoplast thickness are all important factors affecting gradient formation. Among all tested variables, regulation of apoplastic pH was the most important to enable the formation of a lateral auxin gradient. To test this prediction, we interfered with the activity of plasma membrane H⁺ -ATPases that are required to control apoplastic pH. Our results show that H⁺ -ATPases are indeed important for the establishment of a lateral auxin gradient and phototropism. Moreover, we show that during phototropism, H⁺ -ATPase activity is regulated by the phototropin photoreceptors, providing a mechanism by which light influences apoplastic pH

Experimental infections of different carp strains with the carp edema virus (CEV) give insights into the infection biology of the virus and indicate possible solutions to problems caused by koi sleepy disease (KSD) in carp aquaculture

Outbreaks of koi sleepy disease (KSD) caused by carp edema virus (CEV) may seriously affect populations of farmed common carp, one of the most important fish species for global food production. The present study shows further evidence for the involvement of CEV in outbreaks of KSD among carp and koi populations: in a series of infection experiments, CEV from two different genogroups could be transmitted to several strains of naïve common carp via cohabitation with fish infected with CEV. In recipient fish, clinical signs of KSD were induced. The virus load and viral gene expression results confirm gills as the target organ for CEV replication. Gill explants also allowed for a limited virus replication in vitro. The in vivo infection experiments revealed differences in the virulence of the two CEV genogroups which were associated with infections in koi or in common carp, with higher virulence towards the same fish variety as the donor fish. When the susceptibility of different carp strains to a CEV infection and the development of KSD were experimentally investigated, Amur wild carp showed to be relatively more resistant to the infection and did not develop clinical signs for KSD. However, the resistance could not be related to a higher magnitude of type I IFN responses of affected tissues. Despite not having a mechanistic explanation for the resistance of Amur wild carp to KSD, we recommend using this carp strain in breeding programs to limit potential losses caused by CEV in aquaculture