Use Of Co2 Laser In Lingual And Labial Frenectomy

Ankiloglossia or frenum lingual alteration leads to important tongue dysfunction, which, besides discomfort and pain during function, is generally responsible for the difficulty to express specific phonemes. In other cases, a heavy muscular abnormal attachment of labial frenum can promote clinical changes. In such case, an eventual orthodontic therapy is indicated and aesthetic alteration is observed. In both cases, surgical removal is indicated. The surgery, for prevention purposes, must be done as soon as possible, but considering that the majority of patients are young (5-14 years old), difficulties during surgery are expected to occur. Correction of speech or orthodontic dysfunction in advanced ages is much more complex and difficult than in childhood. In the present work we demonstrate that the use of CO2 lasers in these cases are advantageous and simple. The laser energy causes the tissue of the frenum to open in the classic shape with no bleeding and no need for suture, reducing the risk of cross-contamination and of postoperative infection. Scarring and other complications are also minimized. A CO2 laser (continuous, 8 W, 10.6 micrometers) was used assisted with local anesthesia. The major advantage of laser is the possibility of its application in early ages, preventing further problems.391011712

Interprofessional education in Brazil: Building synergic networks of educational and healthcare processes

Univ Fed Rio Grande do Norte, Escola Multicampi Ciencias Med, Caico, BrazilUniv Fed Sao Paulo, Dept Saude Educ & Soc, Santos, BrazilPontificia Univ Catolica Rio Grande do Sul, Fac Serv Social, Porto Alegre, RS, BrazilUniv Fed Minas Gerais, Dept Fisioterapia, Belo Horizonte, MG, BrazilUniv Estado Rio Grande do Norte, Dept Enfermagem, Campus Profa Maria Elisa de Albuquerque Maia, BR-59900000 Pau Dos Ferros, BrazilUniv Estadual Paulista, Fac Med Botucatu, Dept Saude Publ, Botucatu, SP, BrazilUniv Sao Paulo, Escola Enfermagem, Sao Paulo, BrazilUniv Kingston, Ctr Hlth & Social Care Res, London, EnglandUniv London, London, EnglandUniv Fed Sao Paulo, Dept Saude Educ & Soc, Santos, BrazilWeb of Scienc

O USO DO ENTEROCLISMA NO PREPARO PARA O PARTO: ANÁLISE DE SUAS VANTAGENS E DESVANTAGENS

This study aimed at investigating the effects of intestinal cleansers on fecal contamination during labor and delivery amongst women assisted in a university hospital. The subjects were 90 pregnant women (43 normal births, 27 forceps and 20 caesarean sections). The population was divided in two randomized groups: with and without the use of intestinal cleansers. It was possible to conclude that intestinal cleansers did not provide faster labor and did note reduce fecal contamination. However, the women who had used an intestinal cleanser tended to regard it as positive.Se investigaron los efectos de los enemas sobre la contaminación fecal durante el trabajo de parto y el parto, entre mujeres atendidas en un hospital-escuela. La población estuvo constituida por 90 mujeres (43 partos normales, 27 con fórceps y 20 por cesárea). Se realizó un ensayo clínico aleatorio, se consideraron grupos: con y sin enemas. Se puede concluir que el enema no disminuyó el tiempo de trabajo de parto y no redujo la contaminación fecal. Aun así, las mujeres, que realizaron el enema tuvieron opinión más favorable sobre éste que aquellas que no lo realizaron.Investigaram-se os efeitos do enteroclisma sobre a contaminação fecal, durante o trabalho de parto e parto, entre mulheres atendidas num hospital-escola. A população foi constituída por 90 parturientes (43 partos normais, 27 fórceps e 20 cesáreas). Realizou-se um ensaio clínico randomizado, considerando-se dois grupos: com e sem enteroclisma. Pode-se concluir que o enteroclisma não abreviou o tempo de trabalho de parto e não reduziu a contaminação fecal. Entretanto, as mulheres que realizaram o enteroclisma tiveram opinião mais favorável sobre ele do que as que não o realizaram

AVALIAÇÃO DO PERFIL SENSORIAL DE ALIMENTO COM SOJA SABOR LARANJA

Três produtos comerciais de alimento com soja sabor laranja (A, B e C), acondicionados em embalagem longa vida, adquiridos no mercado foram avaliados sensorialmente. A Análise Descritiva Quantitativa (ADQ) foi utilizada para traçar os perfis sensoriais, avaliando-se doze atributos levantados por 10 julgadores selecionados e treinados. A aceitação dos produtos foi avaliada por 30 potenciais consumidores mediante Teste Afetivo, realizado em laboratório, utilizando-se escala hedônica estruturada mista de 9 pontos. Os resultados da ADQ foram submetidos à Análise de Variância (ANOVA), Teste de Média de Tukey e Análise de Componentes Principais, já os resultados do Teste Afetivo (Aceitação) foram analisados pela ANOVA e pelo Teste de Média de Tukey. Os produtos A e B ficaram mais próximos entre si, o produto C diferiu do produto B (significativamente ao nível de 5%) em todos os atributos, com exceção da doçura e amargor. Os produtos A e C diferiram em todos os atributos, com exceção do residual de edulcorante e viscosidade. No teste afetivo, o produto A obteve as maiores médias e notas dos julgadores, não diferindo do produto B em nenhum dos atributos analisados. SENSORY PROFILE EVALUATION OF SOY FOOD PRODUCT WITH ORANGE FLAVOR Abstract Three commercial soy food products with orange flavor (A, B and C) conditioned in long life packing acquired in the market were sensory evaluated. Quantitative Descriptive Analysis (QDA) was used to trace the sensorial profiles evaluating 12 attributes raised through 10 selected and trained panelists. The acceptance of these products was evaluated by 30 potential consumers in a consumer test carried out in laboratory by utilizing mixed hedonic category of 9 points. The results of QDA was submitted to the Analysis of Variance (ANOVA), Tukey test of averages and Principal Components Analysis, although the results of the consumer test were analyzed by ANOVA and by the Tukey test of averages. The products A and B varied between each other and product C differs from product B significantly at 5% level in all attributes with the exception of sweetness and bitterness taste. The products A and C differed in all attributes with the exception to the artificial sweetener aftertaste and viscosity. In the Consumer test the product A received the greater average scores of the panelists presenting no difference from product B in none of the analyzed attributes

Abdominal hernia repair with bovine pericardium seeded with mesenchymal stem cells in Wistar rats

Background: Biological membranes demonstrate superiority over synthetic ones for its biocompatibility and strength in the reduction of abdominal hernias. Recents tissue engineering researches add mesenchymal stem cells to biological membranes with the purpose of obtaining additional cellular proliferation and consequent muscle regeneration, using biological membranes as cellular scaffolds. This article aimed to study the infl uence of mesenchymal stem cells in muscle regeneration in abdominal hernias, reduced with biological membranes. Materials, Methods & Results: Adult Wistar rats underwent abdominal hernia-inducing. They were divided into two groups as to the form of treatment for the reduction of hernia: stem cells associated with biological membranes or only biological membranes. After the treatment the macro and microscopic reviews were carried out in days seven, 14 and 60 postoperatively. Preparation of bovine pericardium with glycerin 98% presented effi ciency in decellularization and conservation, maintaining its strength and avoiding bacterial growth. The mesenchymal stem cells obtained from bone marrow of adult Wistar rats, had capacity of proliferation. The majority of the cells was positive for the expression of surface antigens CD44, CD29 and CD99 and was negative for CD 34. In the differentiation trials, the same cells were able to differentiate into adipocytes and osteocytes. With 24 h from co-cultivating adhesion of mesenchymal stem cells in the membranes was observed. There was no foreign body reaction or contamination of surgical wounds and there was intense postoperative neovascularization on seven days. All animals presented omentum adherence, but no adherence to other organs. There was no statistically difference for the different times in macroscopic assessment: deposition of fi brous tissue, implant integration. The same occurred with the microscopic evaluations between the different treatment groups. The groups of immediate and later repair presented different responses to treatment. Discussion: The use of rats as animal model was satisfactory, being suitable for surgical procedures and assessments of the abdominal cavity. The different results obtained between groups of immediate repair and late repair corroborate with the idea that there is difference between induction and repair models in the same surgery or in different surgeries with the time interval between the two, suggesting the need for methodologies that simulate the hernias chronicity. The cells used were classifi ed as mesenchymal stem cells, because it met all the criteria of Mesenchymal and Tissue Stem Cell Committee of the International Society of Celullar Therapy. The membranes conserved with glycerin 98% demonstrated biocompatibility, because there was no rejection or necrosis, infection or exacerbated infl ammation. However the muscle regeneration was not obtained over the membranes - and the methodological difference in other latest experiments about the membranes decellularization and the co-cultivating - can leads to conclusion that the cells attached to membranes were insuffi cient in number to obtain the desired result. These results suggest the need of new research studies or co-cultivating times and decellularization methods of bovine pericardium for association with mesenchymal stem cells

Genomic investigations of unexplained acute hepatitis in children

Since its first identification in Scotland, over 1,000 cases of unexplained paediatric hepatitis in children have been reported worldwide, including 278 cases in the UK1. Here we report an investigation of 38 cases, 66 age-matched immunocompetent controls and 21 immunocompromised comparator participants, using a combination of genomic, transcriptomic, proteomic and immunohistochemical methods. We detected high levels of adeno-associated virus 2 (AAV2) DNA in the liver, blood, plasma or stool from 27 of 28 cases. We found low levels of adenovirus (HAdV) and human herpesvirus 6B (HHV-6B) in 23 of 31 and 16 of 23, respectively, of the cases tested. By contrast, AAV2 was infrequently detected and at low titre in the blood or the liver from control children with HAdV, even when profoundly immunosuppressed. AAV2, HAdV and HHV-6 phylogeny excluded the emergence of novel strains in cases. Histological analyses of explanted livers showed enrichment for T cells and B lineage cells. Proteomic comparison of liver tissue from cases and healthy controls identified increased expression of HLA class 2, immunoglobulin variable regions and complement proteins. HAdV and AAV2 proteins were not detected in the livers. Instead, we identified AAV2 DNA complexes reflecting both HAdV-mediated and HHV-6B-mediated replication. We hypothesize that high levels of abnormal AAV2 replication products aided by HAdV and, in severe cases, HHV-6B may have triggered immune-mediated hepatic disease in genetically and immunologically predisposed children