Vaginal sampling of amniotic fluid in pPROM: a new technique for early detection of subclinical chorioamnionitis?

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Experimental Chronic Exposure of the Foraminifer Pseudotriloculina rotunda to Zinc

Miliolid (calcareous imperforated test) foraminifera have large diffusion all over the world in a wide range of marine environments, but their distributional pattern in relation to heavy metal pollution is not clearly understood yet. The aim of this study was to better understand the biological response of the miliolid species Pseudotriloculina rotunda to experimental chronic exposure at several zinc concentrations. The duration of the experiment was 10 weeks, and six different concentrations of zinc were tested between 0 and 100 mg/L. Increasing zinc concentrations led to increasing delay or to complete cease of the new chambers’ construction, with consequences on growth rates and affected vitality and biomass variations at medium to high concentrations. Moreover, our results showed that, even at high concentrations, zinc did not cause macroscopic test deformities due to anomalous arrangements of chambers

Contribution of Soft-shelled Monothalamous Taxa to Foraminiferal Assemblages in the Adriatic Sea

Monothalamous foraminifera with organic and agglutinated test walls (‘‘allogromiids’’ sensu lato) deserve attention because of their importance in deep-sea and shallow-water soft-bottom communities and their crucial phylogenetic position at the base of the foraminiferal evolutionary tree. However, our knowledge of the biodiversity and ecology of monothalamous foraminifera is very incomplete and geographically patchy. Here, we present a short review based on the available data on monothalamous, soft-walled foraminiferal taxa from the Adriatic Sea in response to several environmental parameters (i.e., organic matter, oxygen, grain size, depth). The main results of the studies provide evidence of the importance of these foraminiferal taxa in this shallow, temperate latitude area;they represent a start for the identification of soft-shelled monothalamous morphotypes that could be potential bioindicators of environments influenced by inputs of fresh waters, increasing eutrophication and consequent fluctuations in bottom-water oxygenation. The contribution of this soft-shelled component to living benthic foraminiferal assemblages appears not negligible and excluding it from foraminiferal studies can potentially lead to a loss of ecological information. The study, therefore, provide an atlas of the Adriatic soft-shelled foraminiferal taxa in order to 1) encourage the species-level description, if possible, or alternatively a basic morphotype characterization, 2) facilitate future comparisons of taxa from similar settings, 3) promote their potential use in future biomonitoring investigations together with the hard-shelled foraminifera

Technical Note: Determination of the metabolically active fraction of benthic foraminifera by means of Fluorescent In Situ Hybridization (FISH)

Benthic foraminifera are an important component of the marine biota, but protocols for investigating their viability and metabolism are still extremely limited. Classical studies on benthic foraminifera have been based on direct counting under light microscopy. Typically, these organisms are stained with Rose Bengal, which binds proteins and other macromolecules, but does not allow discrimination between viable and recently dead organisms. The fluorescent in situ hybridization technique (FISH) represents a new and useful approach to identify living cells possessing an active metabolism. Our work is the first test of the suitability of the FISH technique, based on fluorescent probes targeting the 18S rRNA, to detect live benthic foraminifera. The protocol was applied on <I>Ammonia</I> group and Miliolids, as well as on agglutinated polythalamous (i.e., <I>Leptohalysis scottii</I> and <I>Eggerella scabra</I>) and soft-shelled monothalamous (i.e., <I>Psammophaga</I> sp. and saccamminid morphotypes) taxa. The results from FISH analyses were compared with those obtained, on the same specimens assayed with FISH, from microscopic analysis of the cytoplasm colour, presence of pigments and pseudopodial activity. Our results indicate that FISH targets only metabolically active foraminifera, and allows discerning from low to high cellular activity, validating the hypothesis that the intensity of the fluorescent signal emitted by the probe is dependent upon the physiological status of cells. These findings support the usefulness of this molecular approach as a key tool for obtaining information on the physiology of living foraminifera, both in field and experimental settings

Evaluation of Glucose Uptake in Normal and Cancer Cell Lines by Positron Emission Tomography.

To date, there is no definitive demonstration of the utility of positron emission tomography (PET) in studying glucose metabolism in cultured cell lines. Thus, this study was designed to compare PET to more standardized methods for the quantitative assessment of glucose uptake in nontransformed and transformed living cells and to validate PET for metabolic studies in vitro. Human colon and breast carcinoma cell lines and mouse embryo fibroblasts were evaluated for [ 18 F]fluorodeoxyglucose ([ 18 F]FDG) uptake by PET and autoradiography and 2-deoxyglucose (2-DG) incorporation by colorimetric assay and analyzed for the radiotoxic effects of [ 18 F]FDG and the expression levels of glucose transporters. Indeed, [ 18 F]FDG incorporation on PET was comparable to [ 18 F]FDG uptake by autoradiography and 2-DG incorporation by colorimetric assay, although radiotracer-based methods exhibited more pronounced differences between individual cell lines. As expected, these data correlated with glucose transporters 1 to 4 and hexokinase II expression in tumor cell lines and mouse fibroblasts. Notably, [ 18 F]FDG incorporation resulted in low apoptotic rates, with fibroblasts being slightly more sensitive to radiotracer-induced cell death. The quantitative analysis of [ 18 F]FDG uptake in living cells by PET represents a valuable and reproducible method to study tumor cell metabolism in vitro, being representative of the differences in the molecular profile of normal and tumor cell lines

3-Methyl-1,4-dioxo-1,4-dihydro­naphthalen-2-yl 4-amino­benzoate

The crystal structure of the title compound, C18H13NO4, the oxidized form of the drug aminaftone used in venous disease therapy, is characterized by the presence of ribbons of hydrogen-bonded mol­ecules parallel to the [111] crystallographic direction and by stacking inter­actions between rings [centroid–centroid distance between quinone rings = 3.684 (3) Å and between amino­benzoate rings = 4.157 (3) Å] along the ribbons

Older people with hip fracture and IADL disability require earlier surgery

Background: Hip fractures represent a major challenge for physicians as well as society as a whole. Both poor functional status and delay to surgery are well known risk factors for negative outcomes. We hypothesized that the timing of the operation is more important for frail older people than older people without functional limitations before fracture. Methods: We performed a prospective multicenter cohort study on 806 consecutive patients, 75 years of age or older, admitted with a fragility hip fracture to three hospitals in the Emilia-Romagna Region (Italy). All three hospitals had a comanaged care model, and the patients were under the shared responsibility of an orthopedic surgeon and a geriatrician. Results: Functional status assessed as instrumental activities of daily living was an important predictor of survival after 1 year from fracture. After adjusting for confounders, the hazard ratios per 1 point score of increase from 0 to 8 was 1.30 (95% confidence interval 1.19-1.42, p =. 000). Time to surgery increased 1-year mortality in patients with a low instrumental activities of daily living score (hazard ratios per day of surgical delay 1.14, 95% confidence interval 1.06-1.22, p <. 001) and intermediate instrumental activities of daily living score (hazard ratios 1.21, 95% confidence interval 1.09-1.34, p <. 001) but was an insignificant risk factor in functionally independent patients (hazard ratios 1.05 95% confidence interval 0.79-1.41, p =. 706). Conclusions: Surgery delay is an independent factor for mortality in older patients after hip fracture but only for the frail older people with prefracture functional impairment. If our results are confirmed, a more intensive approach should be adopted for older people with hip fractures who have disabilities. © 2012 The Author

Pressurized IntraPeritoneal Aerosol Chemotherapy (PIPAC) Applied to Platinum-Resistant Recurrence of Ovarian Tumor: A Single-Institution Experience (ID: PARROT Trial)

Background: We aimed to investigate the therapeutic efficacy and safety of Pressurized IntraPeritoneal Aerosol Chemotherapy (PIPAC) in platinum-resistant recurrence of ovarian cancer and peritoneal carcinomatosis, while our secondary endpoint was to establish any changes in quality of life estimated via the EORTC QLQ-30 and QLQ-OV28 questionnaires. Methods: In this monocentric, single-arm, phase II trial, women were prospectively recruited and every 28-42 days underwent courses of PIPAC with doxorubicin 2.1 mg/m2 followed by cisplatin 10.5 mg/m2 via sequential laparoscopy. Results: Overall, 98 PIPAC procedures were performed on 43 women from January 2016 to January 2020; three procedures were aborted due to extensive intra-abdominal adhesions. The clinical benefit rate (CBR) was reached in 82% of women. Three cycles of PIPAC were completed in 18 women (45%), and 13 (32.5%) and 9 (22.5%) patients were subjected to one and two cycles, respectively. During two PIPAC procedures, patients experienced an intraoperative intestinal perforation. There were no treatment-related deaths. Nineteen patients showed no response according to the Peritoneal Regression Grading Score (PRGS) and 8 patients showed minor response according to the PRGS. Median time from ovarian cancer relapse to disease progression was 12 months (95% confidence interval [CI] 6.483-17.517), while the median overall survival was 27 months (95% CI 20.337-33.663). The EORTC QLQ-28 and EORTC QLQ-30 scores did not worsen during therapy. Conclusions: PIPAC seems a feasible approach for the treatment of this subset of patients, without any impact on their quality of life. Since this study had a small sample size and a single-center design, future research is mandatory, such as its application in addition to systemic chemotherapy

Brief practical clinical diagnostic criteria for the neurodegenerative diseases in the elderly

In the literature there is need of clinical and instrumental characterization of all neurodegenerative diseases. Particular attention deserves the timing of the onset of motor or cognitive symptoms, which is extremely useful issue giving the frequent overlapping between neurodegenerative diseases. Aim of this review is to provide a description of typical clinical and imaging features of all neurodegenerative diseases, especially idiopathic Parkinson's disease (PD) and Alzheimer's disease (AD). Particular attention will be devoted to the cluster of symptoms at the moment of the diagnosis. Based on early starting symptoms (cognitive or extrapiramidal) we will introduce criteria to differentiate AD from fronto-Temporal Dementia (FTD), Lewy bodies dementia (DLB) and Vascular dementia (VaD), and between PD, Vascular Parkinsonism (VP) and DLB. All these diseases are characterized by cognitive deficits. PD will be suspected if cognitive impairment occurs at least one year after the onset of the motor symptoms while VP and DLB are more likely if cognitive deficits and motor symptoms appear simultaneously. Finally, we will focus on parkinsonian signs plus other motor symptoms at the time of the diagnosis. The presence of cerebellar or pyramidal signs, with falls and autonomic dysfunction, with or without cognitive deficit should help to consider potential causes of atypical parkinsonism including cortical-basal degeneration (CBD), multiple system atrophy (MSA) and progressive supranuclear palsy (PSP)

Epidemiology of conjunctivitis in the emergency department of a reference hospital in Goiânia

Objetivo: Avaliar as características das urgências oftalmológicas atendidas no Pronto Socorro do Instituto de Olhos de Goiânia, com destaque à conjuntivite infecciosa, morbidade de maior incidência neste Serviço. Visa ainda delinear as principais etiologias encontradas dentre as conjuntivites infecciosas e compará-las às encontradas em diversos outros serviços de referência em Oftalmologia, norteando, assim, futuros diagnósticos e tratamentos das patologias infecciosas oculares. Métodos: Estudo transversal e retrospectivo, com 783 pacientes atendidos na emergência oftalmológica do Instituto de Olhos de Goiânia, no período de primeiro de maio a 03 de setembro de 2017. Os dados foram coletados por meio de protocolos baseados nos prontuários físicos da emergência oftalmológica do hospital. Resultados: Observou-se que, quanto às causas diagnósticas, a principal foi a conjuntivite aguda, seguida, em ordem decrescente de incidência, por hordéolo e corpo estranho em superfície ocular, com porcentagens, respectivamente, de 10,98% e 9,96%. Dentre os 783 pacientes admitidos na emergência neste período, 302 foram diagnosticados com conjuntivite, representando uma porcentagem de 38,56% da totalidade dos atendimentos. Dentre essas, 226 foram diagnosticados como sendo de etiologia bacteriana e 38 de etiologia viral. Os números absolutos nos levam a uma porcentagem de 74,8% de conjuntivite bacteriana. Conclusão: A conjuntivite infecciosa representa uma porcentagem substancial dentre as patologias admitidas nos serviços de urgência oftalmológica em todo o mundo. No presente estudo houve ainda prevalência da conjuntivite bacteriana, bem como uma tendência irrefutável à instituição precoce de antibioticoterapia tópica. A alta prevalência desta comorbidade e a dificuldade no diagnóstico clínico da etiologia da infecção reitera a necessidade de realização de maiores estudos na área, a fim de otimizar o diagnóstico e a terapêutica da conjuntivite infecciosa.Objective: Evaluate the epidemiology of ocular emergencies in a Reference Ophthalmological Hospital in Goiânia, with emphasis on acute infectious conjunctivitis, morbidity with a higher incidence in this Service. It aims to delineate the main etiologies found among infectious conjunctivitis and to compare them with those found in several others Ophthalmology reference services, thus guiding future diagnoses and treatments of ocular infectious diseases. Methods: A cross-sectional and retrospective study was conducted with 783 patients seen at the ophtalmological emergency of the Goiânia Institute of Eyes from May 1 to September 3, 2017. Data were collected through protocols based on physical charts of the ophthalmologic emergency of the hospital. Results: The most common ocular emergencies were acute conjunctivitis, followed in decreasing order of incidence by hordeolum and foreign body on ocular surface, with percentages, respectively, of 10.98% and 9.96%, respectively. Among the 783 patients admitted to the emergency room in this period, 302 were diagnosed with conjunctivitis, representing a percentage of 38.56% of the total number of visits. Of these, 226 were diagnosed as having bacterial etiology (74.8%) and 38 (25.2%) as viral etiology. Conclusion: Infectious conjunctivitis represents a substantial percentage of the pathologies admitted to ophthalmological emergency services worldwide. In the present study there was also a prevalence of bacterial conjunctivitis, as well as an irrefutable tendency to the early institution of topical antibiotic therapy. The high prevalence of this comorbidity and the difficulty in the clinical diagnosis of the etiology of the infection reiterates the need for further studies in the area in order to optimize the diagnosis and treatment of infectious conjunctivitis