DETEKSI MALARIA ASIMPTOMATIK DI DESA MANUSAK KECAMATAN KUPANG TIMUR KABUPATEN KUPANG SECARA MIKROSKOPIS DAN ANALISIS MOLEKULER DENGAN PCR

Malaria merupakan salah satu penyakit infeksi parasit yang masih menjadi masalah kesehatan dunia,  terutama di negara-negara beriklim tropis termasuk Indonesia. Pada daerah endemik malaria, proporsi terbesar malaria adalah malaria asimtomatik. Diagnosis malaria secara mikroskopis merupakan gold standard yang direkomendasikan oleh WHO. Tetapi karena parasitemia pada malaria asimtomatis sangat rendah dan sulit untuk diperiksa secara mikroskopis, maka diagnosis molekuler dengan single stepPCR dalam penelitian ini digunakan untuk mengkonfirmasi kebenaran diagnosis mikroskopis tersebut. Desa Manusak merupakan salah satu desa yang berada diwilayah kerja Puskesmas Naibona termasuk daerah endemis malaria di Kabupaten Kupang. Oleh karena itu, pada penelitian ini peneliti tertarik untuk mendeteksi malaria asimptomatik di Desa Manusak Kabupaten Kupang secara mikroskopik dan mengkonfirmasi hasil tersebut pada tingkat submikroskopik yakni dengan analisis molekuler dengan PCR.  Penelitian menggunakan metode deskriptif intervensional. Hasil penelitian ini menunjukan perbedaan yang signifikan dimanapada hasil pemeriksaan mikroskopis tidak ditemukan parasit malaria pada semua apusan darah sedangkan pada pemeriksaan submikroskopis dengan metode PCR ditemukan 1,67% sampel positif mengandung gen malaria. Hasil pemeriksaan PCR dari 120 sampel didapatkan 0,83% positif mengandung gen P.vivax dan 0,83% positif mengandung gen P.malariae dan tidak ditemukan gen P.falciparum, P.ovale maupun gen mixed infectio

Combining tissue engineering with metal scaffolds in orthopaedic to improve osseointegration of endoprothesis

Electron Beam Melting (EBM) technology allows the fabrication of free-formed metal scaffolds, thus creating the possibility of manufacturing patient-specific endo-prostheses. It also allows the production of highly-porous prostheses with an elastic modulus similar to that of bone. The porous can be filled-in with tissue engineering elements (i.e. osteogenic molecules, biomaterials, cells) in order to promote bone ingrowth inside them, thus improving the prostheses osseointegration. For this purpose, the surface of EBM-sintered titanium should permit cell adhesion, growth and differentiation to ensure a good metal-to-tissue interaction. Our goal was to evaluate the osteoconductivity of EBM-manufactured Ti6Al4V porous scaffolds. Porous Ti6Al4V discs were manufactured by EBM-sintering, autoclave-sterilized and seeded with human and rat osteoblasts and mesenchymal stem cells (MSC). Cell adhesion, proliferation and differentiation were assessed by vital staining, MTT assay, RT-PCR and immunostaining techniques. Bone organ-explant culture was used to further assess osteoconductivity at tissue level in vitro. Both osteoblastic and MSC attached to and grew on the titanium discs, covering up the entire metal surface, and even bridging the pores of the scaffold. Collagen type I, osteopontin, and osteocalcin expression confirmed the osseous differentiation of the cells cultured on the titanium discs. Bone explants placed on EBM-sintered titanium alloy spontaneously released cells that covered up the metal surface. Long-term cultured explants strongly adhered to the titanium. EBM-sintered titanium scaffolds promote cell adhesion and can be populated by osteoblastic and MSC, which can normally differentiate towards the osteogenic lineage upon proper stimulation. These osteoconductive properties should promote the osseointegration of EBM-manufactured endoprostheses for bone replacement.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Developing An Entrepreneurship-loaded Productive Learning Model

This study was aimed at obtaining a pratical and effective Entrepreneurship-Loaded Productive Learning model (ELPL model) for graduates of the building construction department of the vocational schools to become entrepreneurs of their expertises as technopreneurs. The study was research and development, focused on developing ELPL model. It was done through the following phases: review of the existing models, needs assessment for model development; third, evaluation of model implementability, revision of the ELPL draft, and limited try-out of the ELPL model in Grade XII of the Wood Construction of Vocational School 2, Pengasih, Kulon Progo. Findings show that the ELPL model is implementable for learning, practical, and effective to develop the entrepreneurship skills of the XII grade students in the Wood Construction Subjects at SMK 2 Pengasih, related to their expertise as technopreneurs. PPBK model is feasible to use in teaching and learning processPENGEMBANGAN MODEL PEMBELAJARAN PRODUKTIF BERMUATAN KEWIRAUSAHAANPenelitian ini bertujuan untuk mengembangkan model pembelajaran kemampuan produktif bermuatan kewirausahaan (model PPBK) yang efektif dalam membekali lulusan SMK Program Keahlian Teknik Bangunan untuk menjadi wirausahawan sesuai dengan bidang keahliannya sebagai technopreneur. Penelitian ini merupakan jenis penelitian pengembangan (Research & Development). Penelitian berorientasi pada pengembangan produk yaitu model PPBK. Penelitian dilakukan melalui lima tahapan yaitu: kajian model pembelajaran kemampuan produktif yang telah dilaksanakan, asesmen kebutuhan pengembangan model, penilaian keterlaksanaan model, revisi draf model PPBK, dan uji coba model PPBK. Uji coba model dilakukan secara terbatas pada Kelas XII Paket Keahlian Teknik Konstruksi Kayu di SMK Negeri 2 Pengasih, Kulon Progo. Hasil penelitian menunjukkan bahwa model PPBK yang dikembangkan terbukti dapat diimplementasikan dalam pembelajaran. Model PPBK praktis dan efektif untuk memberikan bekal kemampuan berwirausaha bagi siswa SMK Kelas XII Paket Keahlian Teknik Konstruksi Kayu di SMK Negeri 2 Pengasih sesuai dengan bidang keahliannya sebagai technopreneur. Perangkat pembelajaran PPBK yang dikembangkan layak diimplementasika

Symbol Message Passing Decoding of Nonbinary Low-Density Parity-Check Codes

We present a novel decoding algorithm for q-ary low-density parity-check codes, termed symbol message passing. The proposed algorithm can be seen as a generalization of Gallager B and the binary message passing algorithm by Lechner et al. to q-ary codes. We derive density evolution equations for the q-ary symmetric channel, compute thresholds for a number of regular low-density parity-check code ensembles, and verify those by Monte Carlo simulations of long channel codes. The proposed algorithm shows performance advantages with respect to an algorithm of comparable complexity from the literature

The internationalisation of the Spanish SME sector

As part of a wider research program, we analysed the theoretical framework and the recent developments of the process of internationalisation (transnationalisation) of the small- and medium-sized enterprises in Spain. The paper highlights the main trends and barriers of this internationalisation process. Methodology included document analyses, interviews, and the analyses of statistical databases

Different population phenologies of Grapholita molesta (Busck) in two hosts and two nearby regions in the NE of Spain

Grapholita molesta is an important pest of stone and pome fruits. In commercial orchards, integrated pest management programs use pheromone traps to monitor the population dynamics of G. molesta and adjust treatments. Phenology models can be used to forecast the population phenology of pests and to help optimise the time point at which to spray the orchards with insecticides. In the present study, the adult population phenologies of G. molesta in two provinces of north-east Spain were studied, as well as their fit to the phenology model most used in both provinces. Weekly captures of adults in pheromone traps through the season were recorded over 5 y in a large number of commercial orchards, and these data were used to determine the number of generations of G. molesta in each province. The results show significant differences between provinces in the generation time, being 97 degree days (DD) shorter in the Lleida province than in the Girona province. In Girona province, four generations were registered, while five were detected in Lleida. As a result of the differences found, the phenology model was not able to predict precisely the population dynamics in the Girona province

Convergence, efficiency and dynamics of new fourth and sixth order families of iterative methods for nonlinear system

[EN] In this work we present a new family of iterative methods for solving nonlinear systems that are optimal in the sense of Kung and Traub’s conjecture for the unidimensional case. We generalize this family by performing a new step in the iterative method, getting a new family with order of convergence six. We study the efficiency of these families for the multidimensional case by introducing a new term in the computational cost defined by Grau-Sánchez et al. A comparison with already known methods is done by studying the dynamics of these methods in an example system.This research has been supported by Ministerio de Ciencia e Innovacion MTM2011-28636-C02-02 and by Vicerrectorado de Investigacion Universitat Politecnica de Valencia PAID-SP-2012-0498 and PAID-SP-2012-0474.Hueso Pagoaga, JL.; Martínez Molada, E.; Teruel, C. (2015). Convergence, efficiency and dynamics of new fourth and sixth order families of iterative methods for nonlinear system. Journal of Computational and Applied Mathematics. 275:420-412. https://doi.org/10.1016/j.cam.2014.06.010S42041227

Hematological profiles of the Malayan sun bear (Heiarctos Malayanus) kept in captivity

Nineteen haematological parameters for the Malayan sun bear which include PCV, RBC count, differential counts for leucocytes, plasma protein, PT and APTT were evaluated. Twenty-six biochemical parameters were analysed namely sodium, potassium, chloride, inorganic phosphate, blood urea nitrogen, creatinine, glucose, cholesterol, total bilirubin, total protein, alanine transferase, alkaline phosphatase, aspartate aminotransferase, craetine kinase, globulin, albumin, globulin-albumin ratio, amylase, lactate dehydrogenase, lipase, lactate, uric acid, triglyceride and gammaglutamyl transferase. Comparing males and females, males showed a significantly higher value for haemaglobin. Leucocytes and segmented neutrophils were significantly higher in sub-adults. Current values showed low haemoglobin and leucocytes compared to previous data. Preliminary data on anticoagulation factors namely PT and APTT were recorded. Biochemistry showed ALT, GGT and CK were significantly higher in males. The sub-adult group showed higher values of ALT, GGT, CK, LDH and albumin. Compared to previous reports, current data showed lower values of calcium, inorganic phosphate, BUN and AST. Additional preliminary data on GGT, amylase, CK, total triglyceride, lipase and lactate were recorded

Enantiopure Indolo[2,3-a]quinolizidines: Synthesis and Evaluation as NMDA Receptor Antagonists

Enantiopure tryptophanol is easily obtained from the reduction of its parent natural amino acid trypthophan (available from the chiral pool), and can be used as chiral auxiliary/inductor to control the stereochemical course of a diastereoselective reaction. Furthermore, enantiopure tryptophanol is useful for the syntheses of natural products or biological active molecules containing the aminoalcohol functionality. In this communication, we report the development of a small library of indolo[2,3-a]quinolizidines and evaluation of their activity as N-Methyl D-Aspartate (NMDA) receptor antagonists. The indolo[2,3-a]quinolizidine scaffold was obtained using the following key steps: (i) a stereoselective cyclocondensation of (S)- or (R)-tryptophanol with appropriate racemic -oxoesters; (ii) a stereocontrolled cyclization on the indole nucleus. The synthesized enantiopure indolo[2,3-a]quinolizidines were evaluated as NMDA receptor antagonists and one compound was identified to be 2.9-fold more potent as NMDA receptor blocker than amantadine (used in the clinic for Parkinson's disease). This compound represents a hit compound for the development of novel NMDA receptor antagonists with potential applications in neurodegenerative disorders associated with overactivation of NMDA receptors

Distribución temporal de las características Fisico-Químicas y Biológicas de las aguas de algunos ecosistemas acuáticos del Baio Guadalquivir (SW. España) a lo largo de un ciclo anual

A lo largo de un ciclo anual se estudia la variación temporal de cuatro parámetros físicos: temperatura, transparencia, nivel y del agua; ocho parámetros quirnicos: cloruros, sólidos disueltos, pH, reserva alcalina, oxígeno disuelto, fósforo, nitrógeno y silicio; dos parámetros biológicos: clorofila <> e indice de Pigmentos D430/D665, en cuatro ecosistemas acuáticos de gran interés en el área del Bajo Guadalquivir: Lucio del Aro y Caño Travieso( marisma), Laguna de santa Olalla (arenas de la zona peridunar) y Laguna de Medina (endorreismo bético). Los tres primeros medios acuáticos están localizados dentro del Parque Nacional de Doñana. El cicIo estudiado corresponde a los años 1977 y 1978. Se obtienen valores medios y de dispersión de los paretros ambientales considerados, así como la evolución de los mismos a lo largo del período del estudio. Los resultados se interpretan en relación con las interacciones dinámicas entre los componentes abióticos y bióticos del ecosistema.On a étudié, pendant un cycle annuel, la variation temporelle de quatre paramétres physiques: tempera ture, transparence, niveau et profondeur de l'eau huit paramétres chimiques: chlorures, solidez dissous, pH, reserve alcaline, oxygéne dissous, phospore, nitrogéne et silice, et deux paramétres biologiques: chlorophylle «a» et index de pigments Dzsub>430/P665, dans quatre écosystémes aquatiques de gran intérét, dans la zone du Bas-Guadalquivir: Lucio del Aro et Caño Travieso (marais), Lacune de Santa Olalla (sables de la zone peridunaire) et Lacune de Medina (secteur endoréique bétique). Les trois premiers appartiennent au territoire occupé par le Pare National de Doñana. La période d'étude a compris le cycle annuel entre les années 1977-1978. On a obtenu les caleurs moyennes et de dispersión des paramétres du milieu consideres, ainsi que leur évolution pendant la période d'étude. Les résultats peuvent étre interpretes en rapport aux interactions dynamiques dans les composantes abiotiques et biotiques de l'écosystéme