Natural β-chitin-protein complex film obtained from waste razor shells for transdermal capsaicin carrier

In the literature, the produced β-chitin samples are in powder or flake forms but there is no natural β-chitin based film. Also, the commercially available transdermal patches are produced from synthetic polymers. In this regard,we produced natural β-chitin-protein complex (CPC) film fromthewaste shells of Ensis spp. The obtained natural filmwas characterized by FTIR, TGA and SEM. Additionally, swelling, thickness, contact angle and antioxidant tests were done to learn more about the films. After production and characterization of the film, capsaicin, which is commonly used for pain reliefwas loaded into the film. The loading capacitywas recorded as 5.79%. The kineticmodelswere studied in three different pH, then the resultswere fittedwith Higuchimodelwith high correlation at pH 7.4. After considering all the obtained results, the capsaicin loaded CPC film may be an alternative candidate for transdermal patch instead of the synthetic ones.info:eu-repo/semantics/publishedVersio

Understanding the effects of copolymerized cellulose nanofibers and diatomite nanocomposite on blend chitosan films

Chitosan films lack various important physicochemical properties and need to be supplemented with reinforcing agents to bridge the gap. Herein, we have produced chitosan composite films supplemented with copolymerized (with polyacrylonitrile monomers) cellulose nanofibers and diatomite nanocomposite at different concentrations. The incorporation of CNFs and diatomite enhanced the physicochemical properties of the films. The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%). The antioxidant activity gradually increased with an increasing concentration (1-5% and 10-30%) of copolymerized CNFs/diatomite composite in the chitosan matrix. Moreover, the water solubility decreased from 30% for chitosan control film (CH-0) to 21.06% for films containing 30% diatomite and 5% CNFs (CNFs-D30-5). The scanning electron micrographs showed an overall uniform distribution of copolymerized CNFs/diatomite composite in the chitosan matrix with punctual agglomerations.R. FM. would like to express her gratitude to the Department of Economic Development and Infrastructures of the Basque Government (scholarship of young researchers training) for supporting this research financially. E.R. wants to acknowledge the tenure track position "Biobased materials" part of E2S UPPA supported by the "Investissements d'Avenir" French program managed by ANR (ANR16IDEX0002) . The authors would like to acknowledge the technical and human support provided by SGIker (UPV/EHU/ERDF.EU) and Biotechnology Institute, Ankara University, Turkey. Documen

Understanding the effects of chitosan, chia mucilage, levan based composite coatings on the shelf life of sweet cherry

Sweet cherry (Prunus avium L.) fruits are prone to quality and quantity loss in shelf-life conditions and cold storage due to their short post-harvest life. Until now efforts have been made to extend the shelf life of the sweet cherry. However, an efficient and commercially scalable process remains elusive. To contribute to this challenge, here in this study, biobased composite coatings consisting of chitosan, mucilage, and levan, were applied on sweet cherry fruits and tested for postharvest parameters in both market and cold storage conditions. Results demonstrated that the shelf life of sweet cherries can be extended until the 30th day while retaining important post-harvest properties like decreased weight loss, fungal deterioration, increased stem removal force, total flavonoid, L-ascorbic acid, and oxalic acid. Given the cost-effectiveness of the polymers used, the findings of this study indicate the feasibility of extending the shelf-life of sweet cherries on a larger scale

Newly isolated sporopollenin microcages from Cedrus libani and Pinus nigra as carrier for Oxaliplatin; xCELLigence RTCA-based release assay

Sporopollenin-mediated control drug delivery has been studied extensively owing to its desirable physicochemical and biological properties. Herein, sporopollenin was successfully extracted from C. libani and P. nigra pollens followed by loading of a commonly known anticancer drug Oxaliplatin. Drug loading and physicochemical features were confirmed by using light microscopy, FT-IR, SEM and TGA. For the first-time, real-time cell analyzer system xCELLigence was employed to record the Oxaliplatin loaded sporopollenin-mediated cell death (CaCo-2 and Vero cells) in real time. Both the release assays confirmed the slow release of oxaliplatin from sporopollenin for around 40–45 h. The expression of MYC and FOXO-3 genes has been significantly increased in CaCo2 cell and decreased non-cancerous Vero cell confirming the fact that sporopollenin-mediated control release of oxaliplatin is promoting apoptosis cell death preventing the spread of negative effects on nearby healthy cells. All the results suggested that C. libani and P. nigra can be suitable candidates for the slow delivery of drugs.</p

Extraction and characterization of chitins from coackroach ootheca

Currently researchers are isolating chitins from fungal cell walls [1], crustaceans shells [2], insect cuticles [3], sponges [4] and crabs [5] because of its high biomass (chitin value) and large availability. But still there are many organisms which need a thorough examination for their chitin value. Until now none of the studies are reported on physiochemical properties of cockroach egg shells. For the first time egg shells of three notable and abundantly available species of coackroach (Blatta orientalis, Blatella germanica, Periplaneta americana) were physicochemically investigated for its chitin values using FTIR, chitin content %, SEM. For said study selection of these species was made because of its worldwide distribution and large availibility. Periplaneta americana can be easily found in buildings, restaurants, bakeries, basements, sewers, steam tunnels and drainage systems [6]. Blattella germanica is also a widely distributed urban pest. Adult B.germanica is 1/2 to 5/8 inch long and tan to light brown and can found in houses, apartments, restaurants, hotels. Blatta orientalis is often called water bugs because of their damp and cool habitats such as under sinks, washing machines and in damp basements. Adult B.orientalis is about one inch in length. The egg capsule color and size of B.orientalis is dark reddish-brown and 8 to 10 mm respectively. The dry weight chitin content of Blatta orientalis was determined as 0.66%, Blatella germanica 1.4%, Periplaneta americana 6.61%. The SEM analysis showed that in chitins of coackroach egg shells were found highly fibrous, various crumblier clumpy zones and some highly porous zones were detected. After overall results of SEM we can say that surface morphology of coackroach egg shell’s chitin is highly fibrous along with some highly and rarely porous zones. [...]Gamtos mokslų fakultetasVytauto Didžiojo universiteta

First chitin extraction from Plumatella repens (Bryozoa) with comparison to chitins of insect and fungal origin

Chitin immediately suggests the representatives of the kingdom Fungi, as well as such phyla as Annelida, Mollusca, Porifera, Cnidaria and, mostly, Arthropoda. Although Bryozoa also represents a chitin-containing phylum, no study has been developed yet on the isolation or characterization of the chitin from it. In this study, physiochemical properties of the chitin isolated from Plumatella repens belonging to the phylum Bryozoa was determined for the first time. The chitin structure was also studied comparatively by isolating chitin from an insect species (Palomena prasina) of the phylum Arthropoda, and Fomes fomentarius belonging to the kingdom Fungi. It was observed that the bryozoan chitin was in the α form, as in the arthropod and fungal chitins. The chitin contents in the dry weight of the bryozoan, fungal and insect species were observed to be 13.3%, 2.4%, and 10.8%, respectively. The insect chitin exhibited the highest thermal stability followed by that of the bryozoan and then the fungal chitins. Surface morphologies reveal that the insect and bryozoan chitins were composed of nano fibre and pore structures, whereas the fungal chitin had no pores or fibres. The crystallinity of the insect chitin (CrI = 84.9%) was higher than the bryozoan (CrI = 60.1%) and fungal chitins (CrI = 58.5%)Biologijos katedraVytauto Didžiojo universitetasŽemės ūkio akademij

Fluctuation in physicochemical properties of chitins extracted from different body parts of honeybee

Impact Factor: 4.074It is well known that physicochemical properties of chitin are related with the extraction method. Recently, it was revealed that some physicochemical properties of chitin are also related with taxonomical relationship. For the first time in this study, it was tested how these properties of chitin are affected by different body parts of one organism. The chitins were extracted from five different body parts (head, thorax, abdomen, legs and wings) of honeybee. These chitins were physicochemically characterized and differences among these body parts were identified. Highest chitin content was observed in legs (13.25%) while the lowest from thorax (6.79%). The surface morphologies of the isolated chitin structures from five different body parts were analyzed with SEM, as a result, five different types of surface morphologies were recorded. However, three different types of surface morphologies were observed only in abdomen. Maximum degradation temperatures (DTGmax) of thorax, abdomen, legs and wings were recorded between 359 and 367 °C while DTGmax value of head chitin was found as 308 °CBiologijos katedraVytauto Didžiojo universiteta

Understanding the effects of copolymerized cellulose nanofibers and diatomite nanocomposite on blend chitosan films

Funding Information: R. F-M. would like to express her gratitude to the Department of Economic Development and Infrastructures of the Basque Government (scholarship of young researchers training) for supporting this research financially. E.R. wants to acknowledge the tenure track position “Biobased materials” part of E2S UPPA supported by the “Investissements d'Avenir” French program managed by ANR (ANR-16-IDEX-0002). The authors would like to acknowledge the technical and human support provided by SGIker (UPV/EHU/ERDF.EU) and Biotechnology Institute, Ankara University, Turkey. Funding Information: R. F-M. would like to express her gratitude to the Department of Economic Development and Infrastructures of the Basque Government (scholarship of young researchers training) for supporting this research financially. E.R. wants to acknowledge the tenure track position ?Biobased materials? part of E2S UPPA supported by the ?Investissements d'Avenir? French program managed by ANR (ANR-16-IDEX-0002). The authorswould like to acknowledge the technical and human support provided by SGIker (UPV/EHU/ERDF.EU) and Biotechnology Institute, Ankara University, Turkey. Publisher Copyright: © 2021 The Author(s)Chitosan films lack various important physicochemical properties and need to be supplemented with reinforcing agents to bridge the gap. Herein, we have produced chitosan composite films supplemented with copolymerized (with polyacrylonitrile monomers) cellulose nanofibers and diatomite nanocomposite at different concentrations. The incorporation of CNFs and diatomite enhanced the physicochemical properties of the films. The mechanical characteristics and hydrophobicity of the films were observed to be improved after incorporating the copolymerized CNFs/diatomite composite at different concentrations (CNFs: 1%, 2% and 5%; diatomite: 10% and 30%). The antioxidant activity gradually increased with an increasing concentration (1–5% and 10–30%) of copolymerized CNFs/diatomite composite in the chitosan matrix. Moreover, the water solubility decreased from 30% for chitosan control film (CH-0) to 21.06% for films containing 30% diatomite and 5% CNFs (CNFs-D30-5). The scanning electron micrographs showed an overall uniform distribution of copolymerized CNFs/diatomite composite in the chitosan matrix with punctual agglomerations.Peer reviewe

How Taxonomic Relations Affect the Physicochemical Properties of Chitin

WOS: 000369326900002Chitin specimens from 16 arthropod species (13 of Insecta and 3 of Arachnida) were isolated for the first time using the same method. Fourier Transform Infrared Spectrometry (FTIR), Thermogravimetric Analysis (TGA), X-ray diffraction (XRD), Scanning Electron Microscope (SEM) and elemental analysis have been applied to determine how physicochemical properties of chitin specimens are affected by taxonomic relationship. The characterisation studies revealed that physicochemical nature of the chitin specimens differed greatly and were found partially specific to taxa. Significant differences in the surface morphologies of chitin specimens were observed even in the same order. However, the chitin contents were recorded to be specific to the order in the class Insecta. The highest chitin content was observed in Coleoptera (18.2-25.2 %) followed by Hemiptera (10.6-14.5 %), Odonata (9.5-10.1 %), Hymenoptera (7.8-9.3 %), Diptera (8.1 %), Blattodea (4.7 %). In addition, the crystalline index (CrI) values of chitin specimens from Coleoptera were found to be higher than the other orders in Insecta. This study revealed that the chitin contents and CrI values can be related to taxonomical relationships