Turbulence structure of open channel flows over permeable and impermeable beds : A comparative study

Peer reviewedPublisher PD

Bed-load effects on hydrodynamics of rough-bed open-channel flows

Peer reviewedPublisher PD

The structure of gravel-bed flow with intermediate submergence: a laboratory study

The paper reports an experimental study of the flow structure over an immobile gravel bed in open channel at intermediate submergence, with particular focus on the near-bed region. The experiments consisted of velocity measurements using three-component (stereoscopic) Particle Image Velocimetry (PIV) in near-bed horizontal plane and two-component PIV in three vertical planes that covered three distinctly different hydraulic scenarios where the ratio of flow depth to roughness height (i.e., relative submergence) changes from 7.5 to 10.8. Detailed velocity measurements were supplemented with fine-scale bed elevation data obtained with a laser scanner. The data revealed longitudinal low-momentum and high-momentum "strips'' in the time-averaged velocity field, likely induced by secondary currents. This depth-scale pattern was superimposed with particle-scale patches of flow heterogeneity induced by gravel particle protrusions. A similar picture emerged when considering second-order velocity moments. The interaction between the flow field and gravel-bed protrusions is assessed using cross correlations of velocity components and bed elevations in a horizontal plane just above gravel particle crests. The cross correlations suggest that upward and downward fluid motions are mainly associated with upstream-facing and lee sides of particles, respectively. Results also show that the relative submergence affects the turbulence intensity profiles for vertical velocity over the whole flow depth, while only a weak effect, limited to the near-bed region, is noticed for streamwise velocity component. The approximation of mean velocity profiles with a logarithmic formula reveals that log-profile parameters depend on relative submergence, highlighting inapplicability of a conventional "universal'' logarithmic law for gravel-bed flows with intermediate submergence

Frequency-wavenumber spectrum of the free surface of shallow turbulent flows over a rough boundary

Data on the frequency-wavenumber spectra and dispersion relation of the dynamic water surface in an open channel flow are very scarce. In this work, new data on the frequency-wavenumber spectra were obtained in a rectangular laboratory flume with a rough bottom boundary, over a range of subcritical Froude numbers. These data were used to study the dispersion relation of the surface waves in such shallow turbulent water flows. The results show a complex pattern of surface waves, with a range of scales and velocities. When the mean surface velocity is faster than the minimum phase velocity of gravity-capillary waves, the wave pattern is dominated by stationary waves that interact with the static rough bed. There is a coherent three-dimensional pattern of radially propagating waves with the wavelength approximately equal to the wavelength of the stationary waves. Alongside these waves, there are freely propagating gravity-capillary waves that propagate mainly parallel to the mean flow, both upstream and downstream. In the flow conditions where the mean surface velocity is slower than the minimum phase velocity of gravity-capillary waves, patterns of non-dispersive waves are observed. It is suggested that these waves are forced by turbulence. The results demonstrate that the free surface carries information about the underlying turbulent flow. The knowledge obtained in this study paves the way for the development of novel airborne methods of non-invasive flow monitoring

Process evaluation for complex interventions in primary care: understanding trials using the normalization process model

Background: the Normalization Process Model is a conceptual tool intended to assist in understanding the factors that affect implementation processes in clinical trials and other evaluations of complex interventions. It focuses on the ways that the implementation of complex interventions is shaped by problems of workability and integration.Method: in this paper the model is applied to two different complex trials: (i) the delivery of problem solving therapies for psychosocial distress, and (ii) the delivery of nurse-led clinics for heart failure treatment in primary care.Results: application of the model shows how process evaluations need to focus on more than the immediate contexts in which trial outcomes are generated. Problems relating to intervention workability and integration also need to be understood. The model may be used effectively to explain the implementation process in trials of complex interventions.Conclusion: the model invites evaluators to attend equally to considering how a complex intervention interacts with existing patterns of service organization, professional practice, and professional-patient interaction. The justification for this may be found in the abundance of reports of clinical effectiveness for interventions that have little hope of being implemented in real healthcare setting

Measurement of inositol 1,4,5-trisphosphate in living cells using an improved set of resonance energy transfer-based biosensors

Improved versions of inositol-1,4,5-trisphosphate (InsP3) sensors were created to follow intracellular InsP3 changes in single living cells and in cell populations. Similar to previous InsP3 sensors the new sensors are based on the ligand binding domain of the human type-I InsP3 receptor (InsP3R-LBD), but contain a mutation of either R265K or R269K to lower their InsP3 binding affinity. Tagging the InsP3R-LBD with N-terminal Cerulean and C-terminal Venus allowed measurement of Ins P3 in single-cell FRET experiments. Replacing Cerulean with a Luciferase enzyme allowed experiments in multi-cell format by measuring the change in the BRET signal upon stimulation. These sensors faithfully followed the agonist-induced increase in InsP3 concentration in HEK 293T cells expressing the Gq-coupled AT1 angiotensin receptor detecting a response to agonist concentration as low as 10 pmol/L. Compared to the wild type InsP3 sensor, the mutant sensors showed an improved off-rate, enabling a more rapid and complete return of the signal to the resting value of InsP3 after termination of M3 muscarinic receptor stimulation by atropine. For parallel measurements of intracellular InsP3 and Ca2+ levels in BRET experiments, the Cameleon D3 Ca2+ sensor was modified by replacing its CFP with luciferase. In these experiments depletion of plasma membrane PtdIns(4,5)P2 resulted in the fall of InsP3 level, followed by the decrease of the Ca2+-signal evoked by the stimulation of the AT1 receptor. In contrast, when type-III PI 4-kinases were inhibited with a high concentration of wortmannin or a more specific inhibitor, A1, the decrease of the Ca2+-signal preceded the fall of InsP3 level indicating an InsP3-, independent, direct regulation of capacitative Ca2+ influx by plasma membrane inositol lipids. Taken together, our results indicate that the improved InsP3 sensor can be used to monitor both the increase and decrease of InsP3 levels in live cells suitable for high-throughput BRET applications. © 2015, Public Library of Science. All rights reserved

Simultaneous measurements of concentration and velocity with combined PIV and planar LIF in vegetated open-channel flows

River hydrodynamicsOverbank flows and vegetatio

Coherent turbulence structure generated by wind-induced water waves

River engineeringInnovative field and laboratory instrumentatio

Large-eddy simulation of two-dimensional dunes in a steady, unidirectional flow

We performed large-eddy simulations of the flow over a typical two-dimensional dune geometry at laboratory scale (the Reynolds number based on the average channel height andmean velocity is 18,900) using the Lagrangian dynamic eddy-viscosity subgrid-scale model. The results are validated by comparison with simulations and experiments in the literature. The flowseparates at the dune crest, generating a shear layer that plays a crucial role in the transport of momentum and energy, and the generation of coherent structures. The turbulent kinetic energy budgets show the importance of the turbulent transport and mean-flow advection in the bulk flow above the shear layer. In the recirculation zone and in the attached boundary layers production and dissipation are the most important terms. Large, coherent structures of various types can be observed. Spanwise vortices are generated in the separated shear layer due to the Kelvin-Helmholtz instability; as they are advected, they undergo lateral instabilities and develop into horseshoe-like structures, are tilted downward, and finally reach the surface. The ejection that occurs between the legs of the vortex creates the upwelling and downdrafting events on the free surface known as "boils." Near-wall turbulence, after the reattachment point, is affected by large streamwise Taylor-Görtler vortices generated on the concave part of the stoss side, which affect the distribution of the near-wall streaks

Potential application of mesh-free SPH method in turbulent river flows

A comprehensive review has been completed on the simulation of turbulent flow over rough beds using mesh-free particle models. Based on the outcomes of this review, an improved Smoothed Particle Hydrodynamics (SPH) method has been developed for open channel flows over a rough bed, in which a mixing length model is used for modeling the 2D turbulence and a drag force equation is proposed for treating the boundary shear. The proposed model was applied to simulate a depth-limited open channel flow over a rough bed surface. The results of the velocity profile and shear stress distribution show a good agreement with the experimental data and existing analytical solutions. This work reveals that in order to correctly model turbulent open channel flow over a rough bed, the treatment of both flow turbulence and bed roughness effect is equally important