First person – Agathe Chaigne

First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Agathe Chaigne is first author on ‘ Three-dimensional geometry controls division symmetry in stem cell colonies’, published in JCS. Agathe is a postdoc in the lab of Ewa Paluch at the MRC Laboratory for Molecular Cell Biology (LMCB), University College London, London, UK, investigating the crosstalk between cell division and cell fate transitions during development

Active mesh and neural network pipeline for cell aggregate segmentation

Segmenting cells within cellular aggregates in 3D is a growing challenge in cell biology due to improvements in capacity and accuracy of microscopy techniques. Here, we describe a pipeline to segment images of cell aggregates in 3D. The pipeline combines neural network segmentations with active meshes. We apply our segmentation method to cultured mouse mammary gland organoids imaged over 24 h with oblique plane microscopy, a high-throughput light-sheet fluorescence microscopy technique. We show that our method can also be applied to images of mouse embryonic stem cells imaged with a spinning disc microscope. We segment individual cells based on nuclei and cell membrane fluorescent markers, and track cells over time. We describe metrics to quantify the quality of the automated segmentation. Our segmentation pipeline involves a Fiji plugin that implements active mesh deformation and allows a user to create training data, automatically obtain segmentation meshes from original image data or neural network prediction, and manually curate segmentation data to identify and correct mistakes. Our active meshes-based approach facilitates segmentation postprocessing, correction, and integration with neural network prediction

Response of an artificially blown clarinet to different blowing pressure profiles

Using an artificial mouth with an accurate pressure control, the onset of the pressure oscillations inside the mouthpiece of a simplified clarinet is studied experimentally. Two time profiles are used for the blowing pressure: in a first set of experiments the pressure is increased at constant rates, then decreased at the same rate. In a second set of experiments the pressure rises at a constant rate and is then kept constant for an arbitrary period of time. In both cases the experiments are repeated for different increase rates. Numerical simulations using a simplified clarinet model blown with a constantly increasing mouth pressure are compared to the oscillating pressure obtained inside the mouthpiece. Both show that the beginning of the oscillations appears at a higher pressure values than the theoretical static threshold pressure, a manifestation of bifurcation delay. Experiments performed using an interrupted increase in mouth pressure show that the beginning of the oscillation occurs close to the stop in the increase of the pressure. Experimental results also highlight that the speed of the onset transient of the sound is roughly the same, independently of the duration of the increase phase of the blowing pressure.Comment: 14 page

Intuitive control of rolling sound synthesis

International audienceThis paper presents a rolling sound synthesis model which can be intuitively controlled. To propose this model, different aspects of the rolling phenomenon are explored : physical modeling, perceptual attributes and signal morphology. A source-filter model for rolling sounds synthesis is presented with associated intuitive controls

Artificially decreasing cortical tension generates aneuploidy in mouse oocytes

Human and mouse oocytes’ developmental potential can be predicted by their mechanical properties. Their development into blastocysts requires a specific stiffness window. In this study, we combine live-cell and computational imaging, laser ablation, and biophysical measurements to investigate how deregulation of cortex tension in the oocyte contributes to early developmental failure. We focus on extra-soft cells, the most common defect in a natural population. Using two independent tools to artificially decrease cortical tension, we show that chromosome alignment is impaired in extra-soft mouse oocytes, despite normal spindle morphogenesis and dynamics, inducing aneuploidy. The main cause is a cytoplasmic increase in myosin-II activity that could sterically hinder chromosome capture. We describe here an original mode of generation of aneuploidies that could be very common in oocytes and could contribute to the high aneuploidy rate observed during female meiosis, a leading cause of infertility and congenital disorders

Finite Element Modeling of Airflow During Phonation

International audienceIn the paper a mathematical model of airflow in human vocal folds is presented. The geometry of the glottal channel is based on measurements of excised human larynges. The airflow is modeled by nonstationary incompressible Navier-Stokes equations in a 2D computational domain, which is deformed in time due to vocal fold vibration. The paper presents numerical results and focuses on flow separation in glottis. Quantitative data from numerical simulations are compared to results of measurements by Particle Image Velocimetry (PIV), performed on a scaled self-oscillating physical model of vocal folds

Observation of wave turbulence in vibrating plates

The nonlinear interaction of waves in a driven medium may lead to wave turbulence, a state such that energy is transferred from large to small lengthscales. Here, wave turbulence is observed in experiments on a vibrating plate. The frequency power spectra of the normal velocity of the plate may be rescaled on a single curve, with power-law behaviors that are incompatible with the weak turbulence theory of D{\"u}ring et al. [Phys. Rev. Lett. 97, 025503 (2006)]. Alternative scenarios are suggested to account for this discrepancy -- in particular the occurrence of wave breaking at high frequencies. Finally, the statistics of velocity increments do not display an intermittent behavior

Nonlinear optical memory effect

Light propagating through random media produces characteristic speckle patterns, directly related to the large multitude of scattering events. These complex dynamics remarkably display robustness to perturbation of the incoming light parameters, maintaining correlation in the scattered wavefront. This behavior is known as the optical memory effect. Here we unveil the properties of the nonlinear optical memory effect, which occurs when an optothermal nonlinearity perturbs the random material. The effect is characterized through a series of pump and probe experiments in silica aerogel, in the visible range. This additional degree of freedom further generalizes the memory effect, opening the road to applications based on the nonlinear response of random media. (C) 2019 Optical Society of Americ