Determination of some heavy metal ions by DSPV using Bi modified electrodes

食品安全问题一直是人们关注的焦点。随着工农业生产的迅速发展，食品污染问题越来越严重，其中重金属是最重要的污染物质之一。重金属可以在土壤中积累和动植物体内残留，通过食物链而进入人体内蓄积，构成对人体的危害。人体内重金属含量过量时，会导致各种疾病的发生。食品重金属污染问题已引起全世界的高度重视和深入研究，对不同种类食品和水体中的重金属污染进行监测和分析研究，对于评价食品质量、保护人类健康和维持社会经济可持续发展具有重要的现实意义。 本论文共四章，研究内容主要包括： 第一章，文献综述，内容主要包括重金属污染及危害、重金属对人体的伤害、重金属检测技术的研究与发展、电化学方法在重金属检测中的应用以及...The food security problem has been focused on for a long time. With the development of industrial and agricultural manufacture, food contamination has been becoming a serious problem, especially for heavy metal contaminations. The residence period of most heavy metals in soil, animals or plants is very long. Taken by plants, heavy metals may enter the food chain in significant amounts. Hence, peop...学位：理学硕士院系专业：化学化工学院化学系_分析化学学号：2052008115165

The Cell Bioeffect Study of Several Au-based Nanoparticles

在研究与开发纳米材料的同时，纳米材料的生物效应即生物安全性问题也逐渐成为科研工作者研究的新焦点。作为众多纳米材料重要成员之一的Au纳米粒子，由于制备简单、易于生物修饰等优点在生物医学领域得到了广泛应用，但是有关Au纳米粒子的生物效应研究还缺乏深入系统的研究。本论文瞄准基于Au的纳米粒子的细胞生物效应，开展了一系列的研究。取得的主要成果有：（1）在本实验室建立起小鼠胚胎成纤维细胞培养和传代的稳定方法，然后应用培养的成纤维细胞检测了近红外敏感的Au－Au2S纳米粒子的细胞毒性反应，探讨了小鼠胚胎成纤维细胞培养用于体外毒理学检测的可行性，探索出一种经济、稳定、快速的外来化合物细胞毒性评价方法。（2）...With the fast development of nanotechnology, the bioeffects of the nanomaterials have gradually become a new focus in the research fields flowing the research and exploitation of nanomaterials. Recently, gold nanoparticles as one of the important nanomaterials display several features of straightforward synthesis and easily modification, which make them be widely applied in biomedicine. However, t...学位：工学硕士院系专业：医学院生物医学工程研究中心_☆生物医学工程学号：20044500

Separation and Enrichment of Melamine in Feed by a New Molecular Imprinting Solid-phase Extraction Column

[目的]研制一种新型的分子印迹固相萃取小柱,用于分离富集饲料中的三聚氰胺。[方法]采用本体聚合法制备了三聚氰胺分子印迹聚合物,以该聚合物为填料制作三聚氰胺分子印迹固相萃取(MIP-SPE)小柱,优化了固相萃取条件,应用高效液相色谱(HPlC)法测定浓缩液中的三聚氰胺含量。[结果]自制的MIP-SPE小柱可以从饲料中选择性地分离、富集三聚氰胺,有效地去除饲料中的复杂基质,加标回收率约为95%。MIP-SPE小柱与空白印迹固相萃取小柱(nMIP-SPE)的对比试验表明,前者的萃取效率明显高于后者。[结论]自制的新型三聚氰胺MIP-SPE小柱可用于饲料提取液中的三聚氰胺高选择性分离富集,使用简便,具有广阔的应用前景。[Objective] The research aimed to develop a new molecular imprinting solid-phase extraction column for the separation and enrichment of melamine in feed.[Method] The molecular imprinting polymer of melamine was prepared by noumenon polymeric method.This polymer was used as filler to prepare molecular imprinting polymer solid-phase extraction(MIP-SPE) column of melamine.The solid-phase extraction conditions were optimized and melamine content in concentrated solution was determined by using HPLC.[Result]Melamine could be separated selectively from feed and enriched by home-made MIP-SPE column.It could wipe off complex matrix from feed and the addition recovery rate was 95%.The contrast experiment between MIP-SPE column and NMIP-SPE(non-imprinted polymer-solid phase extraction) column showed that the extraction efficiency of the former was obviously higher than the latter.[Conclusion] The home-made MIP-SPE column could be used to separate and enrich melamine in feed efficiently,with a broad application prospect.厦门市科技计划项目(3502Z20110018

Identification and Biological Characteristics Analysis of a Wild Pleurotus pulmonarius

本研究以野外采集获得的1株野生侧耳属真菌为对象,对其进行rDNA-ITS序列扩增测序并分析后初步确定其为肺形侧耳(Pleurotus; pulmonarius),并与本实验室中现有的秀珍菇菌株序列进行随机扩增多态性DNA; (RAPD)、ISSR、相关序列扩增多态性(SRAP)的综合分析后进一步确定该野生菌株与其他菌株间具有一定的遗传距离,是一株新的秀珍菇菌株。以福; 建省认定的秀珍菇品种秀迪1号为对照菌株,对该菌株进行生物学特性进行研究,结果表明,其菌丝可在温度为5~45°C的范围内生长,最适生长温度为30°; C;菌丝可生长的pH范围为pH; 3~10,最适pH值为7。在含可溶性淀粉、酵母粉为碳氮源的培养基中,菌丝生长速度快,长势好。该野生菌株生长所需的最适碳、氮源及生长条件与对照菌株; 相似,这为其进一步的驯化栽培提供一定的理论基础。In this study, a wild pleurotus strain collected in the forest was; selected as the research object. We sequenced its rDNA-ITS sequence and; analyzed the result to preliminarily classified it as Pleurotus; pulmonarius. Also, the comparision of the random amplified polymorphic; DNA (RAPD), ISSR and sequence related amplified polymorphism (SRAP) with; the strain sequence of other available Pleurotus geesteranus in our; laboratory showed that this strain had certain genetic distance with; other tested strains, which indicated that it was a new Pleurotus; geesteranus strain. The Pleurotus pulmonarius No.l approved by Fujian; province was selected as control group, and its biological; characteristics was analyzed, the result of which showed that its; mycelium could grow at a temperature of 5~45℃, and the optimum growth; temperature was 30°C. The pH range of the mycelium growth was 3 to 10,; and the optimum pH value was 7. In the substrate containing soluble; starch and yeast powder as carbon and nitrogen source, the mycelium grew; fast and good. The most suitable carbon, nitrogen sources and growth; conditions of this wild strain were similar to the control strain, which; provided a theoretical basis for the further domestication and; cultivation of this strain.福州市农业科技项

Development of New Coatings for Solid Phase Microextraction

作为一种样品前处理方法的固相微萃取(SOlId PHASE MICrOEXTrACTIOn,SPME)技术,具有操作方便、快速、灵敏和无需大量有机溶剂的优点,因此在分离分析方面得到了广泛的应用。涂层是SPME技术的核心部分,其性能决定了SPME的性能和应用范围,因此发展新型涂层一直是SPME研究和应用工作的重点。近年来随着涂层材料制备技术的发展,出现了一些新型涂层。这些新型涂层的出现进一步拓宽了SPME技术的应用范围。本文综述了近三年来SPME涂层的研究进展,并着重介绍新型涂层的制备方法和性质。As a kind of sampling preparation method,solid phase microextraction (SPME) has many advantages such as simplicity,versatility,sensitivity and solvent free.It has gained widespread acceptance in separation and analysis.As the key factor of the SPME technique,coatings on SPME fiber determine the performance and application of SPME technique.Consequently,developing new coatings is the most important work for SPME.With the development of preparation methods of SPME coatings,many new coatings have appeared in recent years,and these new coatings expand the application fields of SPME technique.This review summaries the development of new SPME coatings in the past three years,and mainly focuses on the preparation methods and properties of new SPME coatings.福建省科技重点项目(No.2007Y0032)资

3种B族链球菌筛查方法在孕晚期筛查中的应用

目的比较3种B族链球菌(GBS)的筛查方法在孕晚期孕妇GBS筛查中的临床应用价值。方法收集2017年9月至12月在厦门市妇幼保健院产科门诊产检的35～37孕周孕妇阴道/直肠拭子样本1 027例,用GBS运送增菌...厦门市第二批青年创新创业人才项目(2015-A-03

一株高效抑藻放线菌的分离筛选及鉴定

从福建云霄国家红树林自然保护区滩涂沉积物样品中,共分离获得521株纯培养物.通过检测球形棕囊藻(Pha-eocystis globosa)荧光强度计算抑藻率,从521株菌中筛选到27株具有抑藻活性的菌株.在27株抑藻菌中,菌株O3-26对球形棕囊藻具有最高的抑藻率(高达96.71%).菌株O3-26的抑藻谱实验显示,该菌株抑藻活性表现出一定的种属特异性,对硅藻门(Bacillariophyta)的三角褐指藻(Phaeodactylum tricornutum)和中肋骨条藻(Skeletonema costatum)2株测试藻株没有抑制作用,而对绿藻门(Chlorophyta)盐生杜氏藻(Dunaliella salina)和自养小球藻(Chlorella autotrophi-ca)2株藻株具有较强抑藻作用.扫描电镜观察显示,该菌株孢子丝直至螺旋状且孢子表面带刺.生理生化实验显示,该菌株在所得到的大多数培养基上生长良好,在营养琼脂培养基中可以产生水溶性色素;不能在棉子糖作为唯一碳源的培养基上生长.16SrRNA基因相似性分析表明,菌株O3-26属于链霉菌属(Streptomyces),并与灭癌素链霉菌(Streptomycesgancidicus)15412菌株具有最高的同源性(99%).生理生化实验表明,二者之间生理特征存在一定差异.综合形态特征、生理特征以及系统发育分析的结果,鉴定该菌株为灭癌素链霉菌

Synthesis and Applications of Carbon Dots

近年来,由于碳纳米材料具有高的催化活性以及好的稳定性等优点,其在科学、工程以及商业领域都得到了广泛的应用。其中新型“零维“碳纳米材料——碳量子点(CArbOn dOTS,CdS)具有荧光信号稳定、无光闪烁、激发波长和发射波长可调控等独特的光学性质,以及生物毒性小和生物相容性好等优势,逐渐成为碳纳米材料的研究热点,广泛应用于生物成像、生物细胞标记、传感器、光催化、太阳能电池以及发光元件等领域。本文主要综述了CdS的不同合成方法(包括自上而下法和自下而上法)及其应用。In recent years,nanomaterials have made an important impact on diverse science,engineering,and commercial sectors due to their high catalysis,lowcost,and good stability.Acting as a class of ‘zero-dimensional'carbon nanomaterials,carbon dots( CDs) possess unique optical properties of high photostability against photobleaching,tunable excitation and emission wavelength,as well as lowcytotoxicity and good biocompatibility.Therefore,CDs have become a hot subject of carbon nanomaterial in the past decade,not only for its unique properties but also for its applications in various fields such as bioimaging,biolabeling,sensors,photocatalysis,solar cells, light-emitting element and so on.This article reviews the different synthetic methodologies( including two classes: top-down and bottom-up) to achieve good performance of CDs.At the same time,the applications of CDs are also reviewed in the article.国家质检总局科技计划项目(No.2012QK053); 福建省质量技术监督局科技项目(No.3002A91429); 福建省高校产学合作科技重大项目(No.2012H6026); 福建省自然科学基金项目(No.2012D136); 福建省教育厅科技项目(No.JB14180)资助~

Purification and Properties of β-glucosidase from Aspergillus glaucus EU7-22

目的:利用灰绿曲霉Eu7-22发酵产纤维素酶,从中分离到β-葡萄糖苷酶,分析其理化特性,确定其最佳活性条件。方法:灰绿曲霉Eu7-22发酵液离心后,上清液经硫酸铵沉淀、PHEnyl 6 fAST flOW(HIgHSub)疏水层析和SEPHACryl S-200凝胶层析,获得纯化的β-葡萄糖苷酶。结果:纯酶的比活性为5.1 Iu/Mg,得率为13.89%。SdS-PAgE凝胶电泳分析表明该酶是单亚基蛋白,其分子量为56.2 kdA。在PH4.0--6.0范围内,β-葡萄糖苷酶具有较高的稳定性,该酶的最适酶促反应PH为5.0。当β-葡萄糖苷酶在温度低于60℃的缓冲液中温育1 H后,酶活损失不大,表现了较好的稳定性;当该酶在温度高于60℃的缓冲液中温育1 H后,酶活迅速丧失。β-葡萄糖苷酶在70℃时具有最大催化活性。结论:灰绿曲霉Eu7-22发酵产生的β-葡萄糖苷酶具有较高活性,具有分子量较小、最佳催化温度较高的特点。Objective:β-glucosidase produced by Aspergillus glaucus EU7-22 was purified and characterized.Method:The β-glucosidase of Aspergillus glaucus EU722 was purified from ferment supernatant liquid by three steps of purification,ammonium sulfate precipitation(80%,W/V),Phenyl 6 Fast Flow(high sub) column chromatography and Sephacryl S-200 column chromatography,with a specific activity of 5.1 IU/mg and a yield of 13.89%.Result:The purified β-glucosidase was determined as a monomeric protein with a molecular weight of 56.2 kDa.The enzyme exhibited high stability when it was kept in the buffer at pH 4.0～6.0 and at the temperature below 60℃.β-glucosidase exhibited its optimal activity at 70℃.Conclusion: β-glucosidase produced by Aspergillus glaucus EU7-22 with small molecular showed high activity under the optimal conditions.国家“973”计划项目(2010CB732201);国际科技合作重点项目(2009DFA60930)资