抗人DR5单克隆抗体的制备、鉴定及活性分析

目的制备抗人DR5单克隆抗体(mAb),鉴定其特性,并进行生物学活性分析。方法以纯化的可溶性DR5(sDR5)免疫Balb/c小鼠,杂交瘤技术制备抗人DR5mAb;运用ELISA、SDS-PAGE电泳方法测定抗DR5mAb与sDR5结合的特性;Ig亚类ELISA试剂盒鉴定抗DR5mAb亚类;间接ELISA法检测腹水mAb效价;流式细胞仪检测肿瘤细胞表面DR5的表达水平;流式细胞仪检测抗DR5单克隆抗体(mAb)诱导肿瘤细胞凋亡的功能。结果获得1株可分泌抗DR5mAb的杂交瘤细胞系R150。SDS-PAGE电泳检测证实,获得的R150可特异性地识别DR5;R150的Ig亚类为IgGI(λ型);腹水效价为1×106;通过流式细胞仪可敏感地检测到肿瘤细胞表面DR5的表达水平及R150诱导肿瘤细胞凋亡情况。结论获得1株可分泌抗DR5mAb的细胞系R150,抗体具有效价高、特异性强等特点并能有效诱导肿瘤细胞凋亡,具有较好的应用价值

低低跟踪重力卫星高精度微波测距系统数据预处理与分析/Data preprocessing and analysis of high-precision microwave ranging system for low-low satellite-to-satellite tracking mission

This paper studies the data preprocessing and analysis methods of the key microwave ranging system for the low-low satellite-to-satellite tracking gravity mission, and achieve the efficient suppression of the key carrier frequency noise, as well as the elimination and correction of the related interference and deviation. According to the theoretical analysis results, relevant processing and analysis procedures are developed. The residual between the dual one-way ranging data product completed by this research and the official product of GRACE Follow-On is much smaller than the design requirement of the payload, which meets the accuracy requirements of gravity field inversion. Evaluation and analysis methods for electronic noise, system noise and other noise in the final data product are discussed. In this paper, by introducing the frequencydomain analysis method of the spatial inhomogeneity of the ionospheric free electron content, and using the inter-satellite microwave ranging data, the ability to analyse the variation behaviour of free electron content at different spatial scales and its global distribution characteristics is realized, providing data support for the in-depth study of the ionosphere. This research can provide relevant technical accumulation and reference for the data preprocessing and analysis of the microwave ranging system for low-low satellite-to-satellite tracking missions of China

The relationship among psychological flexibility, coping style and job burnout of nurses

目的探讨护士心理灵活性、应对方式与职业倦怠的关系。方法使用接纳与行动问卷第二版(acceptance and action questionnaire 2nd edition,AAQ-Ⅱ)、简易应对方式量表(simplified coping style questionnaire,SCSQ)和护士职业倦怠量表(nursing burnout scale,NBS)对云南省某地市级三级甲等综合性医院694名护士进行测试,运用结构方程模型和Bootstrap方法分析护士心理灵活性、应对方式与职业倦怠间的关系。结果(1)AAQ-Ⅱ总分(21.81±8.23)分、职业倦怠(22.71±6.60)分及其情感耗竭(8.93±2.87)分、人格解体(6.64±2.30)分、成就感降低维度(7.14±2.52)分与应对方式中的消极应对维度(10.86±4.99)分呈正相关(r=0.324~0.510,均P<0.01),与应对方式中的积极应对维度(26.44±5.86)分呈负相关(r=-0.102~-0.143,均P<0.01);(2)结构方程模型分析显示,积极应对和消极应对在心理灵活性与职业倦怠间具有不完全中介效应(χ^2/df=2.30,GFI=0.91,AGFI=0.90,NFI=0.90,IFI=0.93,TLI=0.92,CFI=0.93,RMSEA=0.04);(3)Bootstrap分析显示,积极应对和消极应对的中介效应效果量分别为3.8%、8.9%;应对方式的总中介效应效果量为12.7%。心理灵活性与职业倦怠间的直接效应效果量为87.3%。结论应对方式在心理灵活性与职业倦怠间有较少的中介作用,心理灵活性对职业倦怠的影响以直接影响为主

新疆石河子地区气候变化特征分析/Studv on the trend of climate change in Shihezi[J]

利用1953-2007年的气温、降水资料分析了新疆石河子地区冷暖干湿气候特征的演变规律和突变情况.结果表明:近55 a来石河子地区的气温在20世纪80年代中后期持续升高,90年代以后明显变暖,其中春、冬季升温幅度较大；70年代降水最少,80年代后各季降水持续增加,21世纪后降水增加趋势更加明显.受降水的影响,干燥度经历了一个“偏湿—偏干—偏湿”的变化过程.在西北变暖湿背景下,石河子地区的气温和降水突变都比较明显.各季气温突变比较一致,主要集中在20世纪80年代和90年代；各季降水突变时段不尽相同,在60年代、70年代和80年代都有突变发生；干燥度在60年代和70年代经历了一次变干突变和一次变湿突变

艾比湖流域降水与径流变化特征分析/Trend Analysis of the Precipitation and Runoff in Ebinur Lake Basin[J]

以艾比湖流域的博尔塔拉河（博河）和精河为例,基于1961-2008年的水文气象数据,运用Mann-Kendall非参数检验、线性倾向估计、小波分析等方法,分析流域内2条河流的变化趋势及周期特征,得到以下结论：（1）48年来,艾比湖流域博乐站和精河站降水量均呈现增加的趋势,其中,博乐站的线性倾向斜率为8.54mm/10年;精河站的线性倾向斜率为12.74mm/10年。（2）博河和精河年径流量均呈现极显著的增加趋势,其Z值分别为2.42和2.45,两者均通过а=0.05显著性检验。（3）艾比湖流域年降水量序列的小波系数等值线分布比较密集,存在明显的小波系数高低值中心震荡。两站年降水量在48年的时间序列中存在多个周期。（4）流域内2条河流均有明显的周期性变化,尤其以年代际周期最为显著。在每个周期中径流量又经历了几次＂丰枯＂交替。（5）博河和精河2条河流径流量与降水量的相关系数分别为0.564和0.490,均通过a=0.01的R显著性检验,表明降水是流域径流量的重要补给源之一

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies