Separation and identification of auraptene from grapefruit peel oil

橙油素是广泛存在于柑橘类果实中的天然抗癌活性成分。选用SP70大孔吸附树脂分离纯化葡萄柚精油中的橙油素,该树脂对于橙油素的吸附容量和解吸率分别达到14.53 Mg/g和83.32%,并成功地从树脂床层的洗脱液中结晶分离出橙油素晶体。对所得晶体分别采用差示扫描量热仪(dSC)、紫外吸收光谱(uV)、红外吸收光谱(Ir)、电喷雾质谱(ESI-MS)进行定性分析,分析结果与橙油素标准品相符合。进一步采用高效液相色谱(HPlC)对所得晶体进行定量分析,结果表明所得晶体中橙油素的质量分数可达85%。Auraptene exists widely in the peels and juice sacs of citrus species and is reported to be an effective inhibitor of chemical carcinogenesis in some rodent models.The separation and purification of auraptene from the grapefruit peel oil was performed by SP70 macroporous resin adsorption.The amount adsorbed and the recovery of auraptene were 14.53 mg/g and 83.32% respectively.Auraptene was crystallized out from the concentrated eluate of macroporous resin bed successfully.Differential scanning calorimetric(DSC),ultraviolet spectrum(UV),infrared spectroscopy(IR) and electrospray ionization mass spectroscopy(ESI-MS) were all used to analyze the obtained crystals qualitatively.The analytical results are in accordance with that of auraptene standard.Furthermore,high performance liquid chromatography(HPLC) was utilized to quantify auraptene of the crystals obtained.The mass fraction of auraptene in the obtained crystals can reach 85%.福建省新世纪人才支持项目(0000-X04157

Mechanism of geniposide in improving free fatty acid metabolism in rats with non-alcoholic fatty liver disease

观察栀子苷改善非酒精性脂肪性肝病的效果,从游离脂肪酸探讨栀子苷调节非酒精性脂肪性肝病的作用机制。健康雄性Wistar大鼠40只,随机分为4组:正常组、模型组、栀子苷组和血脂康组,每组大鼠10只,正常组大鼠给予正常大鼠饲料喂养,其余3组大鼠均采用高脂饲料喂养以诱导非酒精性脂肪性肝病,造模时间为8周,从第5周起至第8周末,栀子苷组和血脂康组分别灌服相应的药物。记录大鼠体重、肝湿重、脂肪质量;用相应方法检测肝组织TG,FFA,FAS,AMPK,ACCase及Malonyl-Co A含量,血清CHO,LDL-C的含量,血清AST,ALT的活性;观察肝脏组织肉眼及病理变化(HE染色法)。结果显示,与正常组相比,模型组大鼠的体重;肝湿重;脂肪重;血清CHO,LDL-C,ALT,AST;肝组织TG,FFA,FAS,ACCase及Malonyl-Co A含量皆显著升高(P<0.01);肝组织AMPK活性显著降低(P<0.01),肝组织肉眼外观及病理切片脂肪变性明显,并出现炎症损伤;与模型组相比,栀子苷组大鼠体重、脂肪质量、肝组织FFA含量、血清ALT,AST活性均显著降低(P<0.01),肝湿重,肝组织TG,FAS,ACCase及Malonyl-Co A含量明显降低(P<0.05),肝组织AMPK活性明显增多(P<0.05),肝脏肉眼外观及病理学表现均有所改善;与模型组相比,血脂康组大鼠的肝湿重、脂肪质量、肝组织TG,FFA和血清LDL-C水平明显降低(P<0.05);与血脂康组相比,栀子苷组大鼠的体重、脂肪质量、肝组织FFA含量均显著降低(P<0.01),其他方面无明显差异。结果表明,栀子苷具有显著的改善高脂饮食诱导的大鼠非酒精性脂肪性肝病的药理效应;其改善大鼠非酒精性脂肪性肝病的游离脂肪酸代谢是通过调节"AMPK-ACCase-Malonyl-Co A-FFA"轴来实现的。To observe the effect of geniposide on non-alcoholic fatty liver disease( NAFLD),and discuss the mechanism of geniposide for NAFLD from the aspect of free fatty acid,forty healthy Wistar male rats were randomly divided into normal group,model group,geniposide and Xuezhikang group. The rats in normal group were fed with normal diets,and the rats in other 3 groups were given with high-fat diet for 8 weeks to induce the NAFLD models. From the week 5 to end of week 8,the rats in geniposide and Xuezhikang group were intervened with corresponding medicines. The body weight,liver wet weight,and fat weight of the rats were recorded.Visual and pathological changes in hepatic tissues were observed with HE staining. The contents of TG,FFA,FAS,AMPK,ACCase and Malonyl-Co A in hepatic tissue,contents of CHO and LDL-C in serum and activities of AST and ALT in serum were detected by using corresponding methods. The results showed that the body weight,liver wet weight,and fat weight of the rats,CHO,LDL-C,ALT and AST levels in serum,TG,FFA,FAS,ACCase and Malonyl-Co A levels in hepatic tissues of the rats in model group were significantly higher than those in normal group( P < 0. 01),while AMPK activity was significantly lower than that of the normal group( P < 0. 01),with obvious visual and pathological steatosis in hepatic tissues,and inflammatory injury occurred in model group. Compared with the model group,body weight of the rat,fat weight,levels of FFA in hepatic tissues,ALT and AST activities in serum,liver wet weight,TG,FAS,ACCase and Malonyl-Co A levels were significantly decreased in geniposide group( P < 0. 01),while the AMPK activity in hepatic tissues was significantly increased( P < 0. 05),with improvement in visual and pathological performance.Compared with the model group,liver wet weight,fat weight,TG and FFA levels in hepatic tissues,and LDL-C level in serum were significantly decreased in Xuezhikang group( P < 0. 05). Compared with Xuezhikang group,the body weight of rat,fat weight and FFA level in hepatic tissues were significantly lower in geniposide group( P < 0. 01),but with no significant difference in other aspects. These findings indicated that geniposide was highly effective in improving the pharmacological effect of NAFLD induced by highfat diet,and the mechanism was achieved through AMPK-ACCase-Malonyl-Co A-FFA axis.国家自然科学基金项目(81274155;81503529);; 福建省自然科学基金项目(2014J01374);; 福建省高等学校新世纪人才计划项目;; 厦门市科技计划项目(3502Z20134020);; 福建省中医药科研项目(WZPW201308

用HPLC-MS-MS快速分析和鉴定三尖杉植物内生真菌发酵液中的Brefeldin A

采用HPLC -MS -MS联用技术 ,分析了C56和C65两株具有抗肿瘤活性的三尖杉植物内生真菌发酵液抽提物 ,首次报道了这两株真菌都能产生BrefeldinA(BFA)。采用ESI-MS总离子流跟踪分析HPLC的洗脱液 ,并用低能量的CID -MS -MS(碰撞诱导裂解方式 )进一步确定目标离子峰为BFA分子离子峰 ,这为植物内生真菌发酵液中的有效成分的早期鉴别奠定了基

一种浮动复合型人工鱼巢

一种浮动复合型人工鱼巢，包括浮动框架，在浮动框架的四周设有多个固定装置，并且浮动框架与固定装置之间是采用活动连接，在浮动框架上分布设有多根缆绳，在缆绳的端部固接有沉子，在沉子与浮动框架之间的缆绳上固定连接多个粘附基质，本实用新型提供一种能为产粘性卵鱼类提供产卵场所的浮动复合型人工鱼巢，能有效促进鱼类资源的保护和可持续利用。</p

集美大学2002级福建籍新生肝炎感染现状

目的　了解集美大学 2 0 0 2级福建籍新生甲、乙、戊 3型肝炎感染情况。方法　采用血清流行病学调查方法。结果　学生中抗HAV -IgG ,HBsAg和抗HEV -IgG阳性率分别为 77.0 8% ,1 5 .2 3 % ,1 7.1 5 % ,地区差异明显 ;HAV ,HEV无性别差异 ,而HBV以男性为高 ;农村HAV和HBV感染率均高于城镇 ,而HEV感染率无城乡差别。结论　应该加强对本地区大学生的甲、乙肝预防 ,急需研究出一种有效的戊肝疫

“象”“症”结合论治肿瘤探析

王彦晖教授经过长期临床实践与观察提出了癌症的\"种子土壤说\"新论。强调舌象和脉象是判断肿瘤病机的重要指标,\"象\"与\"症\"的结合更是临床上辨证论治的重要手段。理气疏肝、祛痰化湿、活血化瘀是治疗肿瘤的核心。通过剖析临证治疗肿瘤的实例经验,以期对临床治疗和预防肿瘤具有参考意义。厦门市重大科技计划项目(No.3502Z20100006);;厦门市科技计划高校创新项目(No.3502Z20153027)~

Effects of mowing plus waterlogging on germination and seedling growth of Spartina alterniflora

互花米草(Spartina alterniflora)是我国危害最严重的外来入侵植物之一,探索环保、经济、有效地防治互花米草的技术对保护我国海滩生态环境具有重要意义。本研究通过人工气候室(20~25℃)的盆栽实验,研究刈割与淹水对互花米草萌发和幼苗生长的影响。实验持续4个月,对互花米草地上部分进行了2次刈割,首次刈割是在互花米草生长季结束时,3个月后进行第二次刈割。首次刈割后持续淹水至实验结束,淹水处理设计0、5、10、20 cm四个淹水深度。首次刈割后各淹水处理互花米草根茎上迅速萌发克隆苗,种子的萌发比克隆苗晚约3个月。不同淹水深度对克隆苗的萌发和生长均有抑制作用,克隆苗株数、株高和地上生物量均随淹水深度增加而减少。第二次刈割后各淹水处理均没有再萌发克隆苗,但有少量种子实生苗,其中20 cm水深处理的实生苗数量最少。刈割加淹水可以很好地抑制互花米草的萌发和幼苗生长,据此建议互花米草防治方案为:在春季萌芽前,修筑堤坝,保持淹水20 cm,在营养生长期后期贴地刈割互花米草,继续淹水,第二年重复同样的刈割和淹水。为防止二次入侵,需要在邻近的互花米草分布区同时进行治理。&nbsp;</p

第十八届美国理论与应用力学大会总结

1会议概况2018年6月5—9日,第18届美国理论与应用力学大会(18th U.S. National Congress of Theoretical and Applied Mechanics, USNCTAM2018)在美国芝加哥召开.本次大会由美国力学国家委员会和中国力学学会联合主办,旨在探讨和交流近四年世界范围内在理论和应用力学领域的基础研究、创新技术的最新进展,吸引了来自世界各地的近千名专家学

Optimization of separation methods and culture system of chicken embryonic stem cells in vitro

背景:胚胎干细胞是从动物早期胚胎的内细胞团或原始生殖细胞分离出来的具有发育全能性的一种未分化的无限增殖细胞系。而鸡胚胎干细胞则是从X期鸡胚的胚盘分离而来。目的:优化鸡胚胎干细胞分离方法和离体培养体系。方法:采用滤纸纸环-发环的方法从X期鸡胚分离胚盘细胞,并采用STO细胞作为饲养层和大鼠肝细胞(brl)条件培养基(CM)+细胞因子作为离体培养体系对分离的胚盘细胞进行培养。结果与结论:滤纸纸环-发环法获得的完整胚盘率为75%~85%,克隆形成率约为50%。brl-CM+饲养层培养体系,鸡胚胎干细胞可传至7代,而brl-CM+饲养层+细胞因子培养体系,鸡胚胎干细胞可传至25代。分离到的鸡胚胎干细胞,经碱性磷酸酶染色、SSEA-1染色鉴定,表明鸡胚胎干细胞处于未分化状态。提示,实验不仅优化了鸡胚胎的分离方法,获得完整且杂质少的胚盘,而且进一步优化了鸡胚胎干细胞体外培养体系。BACKGROUND:Embryonic stem cells are undifferentiated permanent cell line derived from inner cell mass cells and primordial germ cells of animal's early embryos.Chicken embryonic stem cells are derived from the blastodermal of a X-stage embryo.OBJECTIVE:To optim the separation method and in vitro cultural system of chicken embryonic stem cells.METHODS:The X-stage chicken embryos were isolated by using a small square of ?lter paper with a hole punched in the center,and the blastodermal cells were isolated by using the hair loop.STO cells were used to make feeder layer;at the same time,BRL-CM and cytokine were also used for chicken embryonic stem cells in vitro cultural system.RESULTS AND CONCLUSION:The filter paper loop and the hair loop could obtain complete the blastoderm,and the successful percentage was 75%-85%.The colony formation rate was about 50%.After culture in the BRL-CM + feeder layer + cytokine culture system,the passage of CES cells is the seventh generation;BRL-CM + feeder layer + cytokines,cultured chicken embryonic stem cells could passage to the 25th generation.Isolated chicken embryonic stem cells were in an undifferentiated state detected by alkaline phosphatase staining and SSEA-1 staining.The findings indicate that this experiment not only optimized the isolation method of chicken embryonic stem cells to obtain complete and pure embryos,but also further improved the in vitro culture system of chicken embryonic stem cells.国家973项目(2009CB941600)资助;国家自然科学基金项目(31072101)资助---