Callus Induction and Plant Regeneration in Callistemon rigidus

以茎段、芽和叶片为材料,探讨了红千层愈伤组织诱导及植株再生的方法.结果表明:红千层的茎段、芽和叶片均可诱导出愈伤组织,通过继代培养可发育成绿色和粉红色2种类型的愈伤组织,其中绿色、致密的愈伤组织可以分化出不定芽;培养基1/2M S+6-BA 1.0 m g/L+NAA 0.1 m g/L适宜愈伤组织不定芽的诱导,在培养基1/2M S+IBA 0.25 m g/L上试管苗的生根率可达95%.Taking stem segment,bud and leaf as materials,this paper made a study of callus induction and plant regeneration in Callistemon rigidus R.Br.The results show that callus can be induced from all types of materials and after being sub-cultured,the callus will develop to two kinds of colors that are pink and green;that the pink callus will be degenerated while the compact green one will induce adventitious buds.The optimal medium for adventitious bud inducing is 1/2 MS+6-BA 1.0 mg/L+NAA 0.1 mg/L and the optimal for the rooting of test-tube plantlets is 1/2MS+IBA0.25 mg/L on which its rate can reach 95 % after being cultured for 40 days.广东省科技计划项目(2004B60302006);; 广州市科技计划项目(004Z3-E0361)的部分研究内

Callus Induction and Plant Regeneration in Callistemon rigidus

以茎段、芽和叶片为材料,研究了红千层愈伤组织诱导及植株再生的方法。结果表明:红千层的茎段、芽和叶片均可诱导出愈伤组织,通过继代培养愈伤组织可发育成绿色和粉红色2种类型,其中绿色、致密的愈伤组织可以分化出不定芽。培养基1/2M S+6-BA 1.0 m g/L+NAA 0.1 m g/L适宜愈伤组织不定芽的诱导,在培养基1/2M S+IBA 0.25 m g/L上试管苗的生根率可达95%。Taken stem segment,bud and leaf as materials,the means of callus induction and plant regeneration in Callistemon rigidus R.Br.were studied.The results showed that callus could be induced from all types of the materials,pink and green callus could be gain through subculture,and adventitious buds could be induced from the compacted greens.The optimal medium for inducing adventitious bud was 1/2MS supplemented with 6-BA1.0 mg/L and NAA0.1 mg/L,and the optimal one for test-tube plantlets rooting was 1/2MS supplemented with IBA0.25 mg/L,in which the rooting rate could be up to 95%.广东省科技计划项目(2004B60302006);; 广州市科技计划项目(004Z3-E0361

Stem Culture and Rapid Propagation of Melastoma malabathricum Linn.

广州市科技计划项目(2002Z3-E0291

Seeds Culture of Anoectochilus roxburghii

金线莲种子在培养基1/2MS+6-BA1.0 mg/L+NAA1.0 mg/L上萌发后形成原球茎,原球茎可以直接发育成幼苗,也可以由原球茎产生愈伤组织,再由愈伤组织发育成类原球茎而分化成幼苗。通过类原球茎可以实现大量增殖,在MS+6-BA1.0 mg/L+NAA0.1 mg/L上培养60 d的增殖倍数达到6.7倍。在培养基MS+IBA0.3mg/L上,金线莲的生根率可达到96.0%。Seeds of Anoectochilus roxburghii would germinate and develop to protocorms on the medium of 1/2MS+6-BA1.0 mg/L+NAA1.0 mg/L.The protocorms may develop to seedlings immediately or induce callous and then induce protocorm-liked-bodies which would develop to seedlings also.Anoectochilus roxburghii be rapidly propagated by the later method and 6.7 folds of propagation would get on the medium of MS+6-BA1.0 mg/L +NAA0.1 mg/L after subcultured 60 days.Seedling′s rooting rate was 96.0% on the medium of MS+IBA0.3 mg/L.广东省科技计划项目(2004B60302006);; 广州市科技计划项目(2004Z3-E0361);; 广州市建委项目[(2003)06

ptso42zro2al2o3在正己烷异构化反应中的催化行为

采用连续流动的固定床微反装置考察了Pt/SO42-/ZrO2-Al2O3（PSZA）在正己烷异构化反应中的催化行为。采用NH3-TPD、H2-TPR及TG表征了催化剂的酸性、还原性能及硫物种含量。结果表明，PSZA的初始异构化催化活性几乎不受反应温度的影响，而稳定性则与反应温度密切相关。低温下反应，催化剂在短时间内迅速失活，而提高反应温度可大大提高PSZA的反应稳定性。PSZA具有良好的再生性能，与新鲜催化剂相比，多次再生后的催化剂异构化催化活性基本没有变化。PSZA在低温下的快速失活与其催化活性中心产生的机理有关，而与其硫损失或硫物种的还原无关。在异构化反应过程中，催化剂通过氢溢流可产生强酸活性中心，并在反应过程中不断被消耗；在高温下通过氢溢流不断产生新的强酸中心，使催化活性保持稳定；而低温下氢溢流难以发生，消耗的强酸活性中心不能及时补充，使催化活性下降。

ptso42zro2al2o3在正己烷异构化反应中的催化行为

采用连续流动的固定床微反装置考察了Pt/SO42-/ZrO2-Al2O3（PSZA）在正己烷异构化反应中的催化行为。采用NH3-TPD、H2-TPR及TG表征了催化剂的酸性、还原性能及硫物种含量。结果表明，PSZA的初始异构化催化活性几乎不受反应温度的影响，而稳定性则与反应温度密切相关。低温下反应，催化剂在短时间内迅速失活，而提高反应温度可大大提高PSZA的反应稳定性。PSZA具有良好的再生性能，与新鲜催化剂相比，多次再生后的催化剂异构化催化活性基本没有变化。PSZA在低温下的快速失活与其催化活性中心产生的机理有关，而与其硫损失或硫物种的还原无关。在异构化反应过程中，催化剂通过氢溢流可产生强酸活性中心，并在反应过程中不断被消耗；在高温下通过氢溢流不断产生新的强酸中心，使催化活性保持稳定；而低温下氢溢流难以发生，消耗的强酸活性中心不能及时补充，使催化活性下降。