以茎段、芽和叶片为材料,探讨了红千层愈伤组织诱导及植株再生的方法.结果表明:红千层的茎段、芽和叶片均可诱导出愈伤组织,通过继代培养可发育成绿色和粉红色2种类型的愈伤组织,其中绿色、致密的愈伤组织可以分化出不定芽;培养基1/2M S+6-BA 1.0 m g/L+NAA 0.1 m g/L适宜愈伤组织不定芽的诱导,在培养基1/2M S+IBA 0.25 m g/L上试管苗的生根率可达95%.Taking stem segment,bud and leaf as materials,this paper made a study of callus induction and plant regeneration in Callistemon rigidus R.Br.The results show that callus can be induced from all types of materials and after being sub-cultured,the callus will develop to two kinds of colors that are pink and green;that the pink callus will be degenerated while the compact green one will induce adventitious buds.The optimal medium for adventitious bud inducing is 1/2 MS+6-BA 1.0 mg/L+NAA 0.1 mg/L and the optimal for the rooting of test-tube plantlets is 1/2MS+IBA0.25 mg/L on which its rate can reach 95 % after being cultured for 40 days.广东省科技计划项目(2004B60302006);; 广州市科技计划项目(004Z3-E0361)的部分研究内
