以茎段、芽和叶片为材料,研究了红千层愈伤组织诱导及植株再生的方法。结果表明:红千层的茎段、芽和叶片均可诱导出愈伤组织,通过继代培养愈伤组织可发育成绿色和粉红色2种类型,其中绿色、致密的愈伤组织可以分化出不定芽。培养基1/2M S+6-BA 1.0 m g/L+NAA 0.1 m g/L适宜愈伤组织不定芽的诱导,在培养基1/2M S+IBA 0.25 m g/L上试管苗的生根率可达95%。Taken stem segment,bud and leaf as materials,the means of callus induction and plant regeneration in Callistemon rigidus R.Br.were studied.The results showed that callus could be induced from all types of the materials,pink and green callus could be gain through subculture,and adventitious buds could be induced from the compacted greens.The optimal medium for inducing adventitious bud was 1/2MS supplemented with 6-BA1.0 mg/L and NAA0.1 mg/L,and the optimal one for test-tube plantlets rooting was 1/2MS supplemented with IBA0.25 mg/L,in which the rooting rate could be up to 95%.广东省科技计划项目(2004B60302006);; 广州市科技计划项目(004Z3-E0361
