Fluid-solid transition in unsteady, homogeneous, granular shear flows

Discrete element numerical simulations of unsteady, homogeneous shear flows have been performed by instantly applying a constant shear rate to a random, static, isotropic assembly of identical, soft, frictional spheres at either zero or finite pressure by keeping constant the solid volume fraction until the steady state is reached. If the system is slowly sheared, or, equivalently, if the particles are sufficiently rigid, the granular material exhibits either large or small fluctuations in the evolving pressure, depending whether the average number of contacts per particle (coordination number) is less or larger than a critical value. The amplitude of the pressure fluctuations is rate-dependent when the coordination number is less than the critical and rate-independent otherwise, signatures of fluid-like and solid-like behaviour, respectively. The same critical coordination number has been previously found to represent the minimum value at which rate-independent components of the stresses develop in steady, simple shearing and the jamming transition in isotropic random packings. The observed complex behaviour of the measured pressure in the fluid-solid transition clearly suggests the need for incorporating in a nontrivial way the coordination number, the solid volume fraction, the particle stiffness and the intensity of the particle agitation in constitutive models for the onset and the arrest of granular flows.Comment: 20 pages, 14 figures, submitted to Granular Matte

Plane shear flows of frictionless spheres: Kinetic theory and 3D soft-sphere discrete element method simulations

We use existing 3D Discrete Element simulations of simple shear flows of spheres to evaluate the radial distribution function at contact that enables kinetic theory to correctly predict the pressure and the shear stress, for different values of the collisional coefficient of restitution. Then, we perform 3D Discrete Element simulations of plane flows of frictionless, inelastic spheres, sheared between walls made bumpy by gluing particles in a regular array, at fixed average volume fraction and distance between the walls. The results of the numerical simulations are used to derive boundary conditions appropriated in the cases of large and small bumpiness. Those boundary conditions are, then, employed to numerically integrate the differential equations of Extended Kinetic Theory, where the breaking of the molecular chaos assumption at volume fraction larger than 0.49 is taken into account in the expression of the dissipation rate. We show that the Extended Kinetic Theory is in very good agreement with the numerical simulations, even for coefficients of restitution as low as 0.50. When the bumpiness is increased, we observe that some of the flowing particles are stuck in the gaps between the wall spheres. As a consequence, the walls are more dissipative than expected, and the flows resemble simple shear flows, i.e., flows of rather constant volume fraction and granular temperature

A transcriptional regulatory mechanism finely tunes the firing of type VI secretion system in response to bacterial enemies

The ability to detect and measure danger from an environmental signal is paramount for bacteria to respond accordingly, deploying strategies that halt or counteract potential cellular injury and maximize survival chances. Type VI secretion systems (T6SSs) are complex bacterial contractile nanomachines able to target toxic effectors into neighboring bacteria competing for the same colonization niche. Previous studies support the concept that either T6SSs are constitutively active or they fire effectors in response to various stimuli, such as high bacterial density, cell-cell contact, nutrient depletion, or components from dead sibling cells. For Serratia marcescens, it has been proposed that its T6SS is stochastically expressed, with no distinction between harmless or aggressive competitors. In contrast, we demonstrate that the Rcs regulatory system is responsible for finely tuning Serratia T6SS expression levels, behaving as a transcriptional rheostat. When confronted with harmless bacteria, basal T6SS expression levels suffice for Serratia to eliminate the competitor. A moderate T6SS upregulation is triggered when, according to the aggressor-prey ratio, an unbalanced interplay between homologous and heterologous effectors and immunity proteins takes place. Higher T6SS expression levels are achieved when Serratia is challenged by a contender like Acinetobacter, which indiscriminately fires heterologous effectors able to exert lethal cellular harm, threatening the survival of the Serratia population. We also demonstrate that Serratia’s RcsB-dependent T6SS regulatory mechanism responds not to general stress signals but to the action of specific effectors from competitors, displaying an exquisite strategy to weigh risks and keep the balance between energy expenditure and fitness costs. IMPORTANCE Serratia marcescens is among the health-threatening pathogens categorized by the WHO as research priorities to develop alternative antimicrobial strategies, and it was also recently identified as one major component of the gut microbiome in familial Crohn disease dysbiosis. Type VI secretion systems (T6SSs) stand among the array of survival strategies that Serratia displays. They are contractile multiprotein complexes able to deliver toxic effectors directed to kill bacterial species sharing the same niche and, thus, competing for vital resources. Here, we show that Serratia is able to detect and measure the extent of damage generated through T6SS-delivered toxins from neighboring bacteria and responds by transcriptionally adjusting the expression level of its own T6SS machinery to counterattack the rival. This strategy allows Serratia to finely tune the production of costly T6SS devices to maximize the chances of successfully fighting against enemies and minimize energy investment. The knowledge of this novel mechanism provides insight to better understand bacterial interactions and design alternative treatments for polymicrobial infections.Fil: Lazzaro, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Feldman, Mario F.. Washington University in St. Louis; Estados UnidosFil: Garcia Vescovi, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentin

Quantum Criticality in a Bosonic Josephson Junction

In this paper we consider a bosonic Josephson junction described by a two-mode Bose-Hubbard model, and we thoroughly analyze a quantum phase transition occurring in the system in the limit of infinite bosonic population. We discuss the relation between this quantum phase transition and the dynamical bifurcation occurring in the spectrum of the Discrete Self Trapping equations describing the system at the semiclassical level. In particular, we identify five regimes depending on the strength of the effective interaction among bosons, and study the finite-size effects arising from the finiteness of the bosonic population. We devote a special attention to the critical regime which reduces to the dynamical bifurcation point in the thermodynamic limit of infinite bosonic population. Specifically, we highlight an anomalous scaling in the population imbalance between the two wells of the trapping potential, as well as in two quantities borrowed from Quantum Information Theory, i.e. the entropy of entanglement and the ground-state fidelity. Our analysis is not limited to the zero temperature case, but considers thermal effects as well.Comment: 13 pages, 10 figure

Serratia marcescens ShlA Pore-Forming Toxin Is Responsible for Early Induction of Autophagy in Host Cells and Is Transcriptionally Regulated by RcsB

Serratia marcescens is a Gram-negative bacterium that thrives in a wide variety of ambient niches and interacts with an ample range of hosts. As an opportunistic human pathogen, it has increased its clinical incidence in recent years, being responsible for life-threatening nosocomial infections. S. marcescens produces numerous exoproteins with toxic effects, including the ShlA pore-forming toxin, which has been catalogued as its most potent cytotoxin. However, the regulatory mechanisms that govern ShlA expression, as well as its action toward the host, have remained unclear. We have shown that S. marcescens elicits an autophagic response in host nonphagocytic cells. In this work, we determine that the expression of ShlA is responsible for the autophagic response that is promoted prior to bacterial internalization in epithelial cells. We show that a strain unable to express ShlA is no longer able to induce this autophagic mechanism, while heterologous expression of ShlA/ShlB suffices to confer on noninvasive Escherichia coli the capacity to trigger autophagy. We also demonstrate that shlBA harbors a binding motif for the RcsB regulator in its promoter region. RcsB-dependent control of shlBA constitutes a feed-forward regulatory mechanism that allows interplay with flagellar-biogenesis regulation. At the top of the circuit, activated RcsB downregulates expression of flagella by binding to the flhDC promoter region, preventing FliA-activated transcription of shlBA. Simultaneously, RcsB interaction within the shlBA promoter represses ShlA expression. This circuit offers multiple access points to fine-tune ShlA production. These findings also strengthen the case for an RcsB role in orchestrating the expression of Serratia virulence factorsFil: Di Venanzio, Gisela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Stepanenko, Tatiana Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Garcia Vescovi, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentin

New linearization and reweighting for simulations of string sigma-model on the lattice

We study the discretized worldsheet of Type IIB strings in the Gubser-Klebanov-Polyakov background in a new setup, which eliminates a complex phase previously detected in the fermionic determinant. A sign ambiguity remains, which a study of the fermionic spectrum shows to be related to Yukawa-like terms, including those present in the original Lagrangian before the linearization standard in a lattice QFT approach. Monte Carlo simulations are performed in a large region of the parameter space, where the sign problem starts becoming severe and instabilities appear due to the zero eigenvalues of the fermionic operator. To face these problems, simulations are conducted using the absolute value of a fermionic Pfaffian obtained introducing a small twisted-mass term, acting as an infrared regulator, into the action. The sign of the Pfaffian and the low modes of the quadratic fermionic operator are then taken into account by a reweighting procedure of which we discuss the impact on the measurement of the observables. In this setup we study bosonic and fermionic correlators and observe a divergence in the latter, which we argue - also via a one-loop analysis in lattice perturbation theory - to originate from the U(1)-breaking of our Wilson-like discretization for the fermionic sector

The Complexity of Poverty. Dickens’s Response to the Poor Law from Oliver Twist to Bleak House

Pochi autori hanno saputo rappresentare la povert\ue0 con la compassione e la sensibilit\ue0 di Dickens, sorprende dunque che rari siano gli studi pubblicati sul rapporto tra Dickens e i poveri del suo tempo. Il presente articolo indaga l\u2019evoluzione del concetto di povert\ue0 nelle opere di Dickens dal giovanile Oliver Twist al pi\uf9 maturo Bleak House. In un momento in cui la riflessione sulla povert\ue0 era dominata dall\u2019agenda della Poor Law Commission, il pensiero che emerge dagli scritti dickensiani vede un\u2019evoluzione da una spontanea empatia di matrice religiosa a una visione pi\uf9 sofisticata della povert\ue0. La stessa poetica dei romanzi dickensiani consente col tempo di penetrare sempre pi\uf9 profondamente la complessit\ue0 della condizione del povero, in aperto contrasto con la semplicistica nozione vittoriana secondo cui i problemi che conducono all\u2019indigenza possono essere ricondotti a due o tre categorie e risolti di conseguenza.Few authors have been able to represent poverty with the intelligence and compassion shown by Dickens. And yet little scholarship has been devoted to the relationship between the Victorian novelist and the paupers of his times. This paper investigates the evolution of the idea of poverty in some of Dickens\u2019s work, from his early novel Oliver Twist to his more mature Bleak House. At a time when the understanding of poverty was dominated by the agenda of the Poor Law Commission, Dickens seems to move from a spontaneous and religious empathy with the poor, to a more sophisticated view of poverty, which he tackles in all its social and human complexity. Indeed Dickens\u2019s poetics allows him to offer a glimpse into the complexity of the poor\u2019s predicament, gainsaying the Victorian notion that indigent people could be sorted into two or three categories and managed accordingly

Muri e mura, architettura e città. Cantieri e struttura urbana a Parma tra XII e XIII secolo

L'analisi delle relazioni tra cantieri architettonici e assetto urbano risulta fondamentale per definire la conformazione e lo sviluppo delle città in epoca medievale. In questo saggio, tramite l'analisi di alcune strutture architettoniche, viene ripercorso lo sviluppo di Parma tra XII e XIII secolo, nell'ampliamento delle mura e nella creazione della platea.The study of the relationship between architecture and urban planning is essential to defining the shape and development of medieval cities. This paper presents the urban development of Parma between the 12th and 13th centuries, through architectural analysis of the churches of San Barnaba and Sant'Andrea in relation to urban planning, the city walls and the creation of the platea