Recording Loss: film as method and the spirit of Orford Ness

This is the author accepted manscript. The final version is available from Routledge via the DOI in this record.This paper explores the use of film as a method to explore themes of change and loss which emerged during recording of archaeological features at Orford Ness, UK. Owned by the National Trust, Orford Ness is an exposed shingle spit off the Suffolk coast recognised for its natural and cultural heritage. The research discussed in this paper engaged with a community archaeology project which has been recording features on the shingle spit as they are altered and erased by erosion and other coastal processes. The authors experimented with film as a method to investigate the work being undertaken by practitioners and volunteers in this dynamic landscape. We conclude that, within interdisciplinary heritage research, experimenting with film as a method facilitates the representation of embodied practices and exposes processes of meaning-making. We frame our discussion about the active production of meaning through an analysis of the way that film engaged with qualities articulated in the National Trust’s Spirit of Place statement for the site.Arts and Humanities Research Counci

Antimicrobial activity of Warbugia ugandensis against gramnegative multi‐drug resistant bacteria

The rise in antibiotic resistance has resulted in decreasing numbers of effective antimicrobial agents available to treat infections caused by multi‐drug resistant (MDR) bacteria. This has necessitated a search for new antimicrobial agents. Herbal remedies may offer alternative treatment options especially because they elicit little or no transferable resistance if used in optimal concentrations. This study evaluated the antimicrobial properties of W. ugandensis against eight multi drug resistant (MDR) Gram‐negative bacterial isolates. The herbal extracts were obtained using methanol as an organic solvent and water as an inorganic solvent. Determination of the Minimum Inhibitory Concentrations (MICs) and the sub‐lethal concentrations of the effective extracts was done using broth inoculation method followed by colony count. The test isolates were habituated in sub‐lethal extract concentrations (SLC) for 72 h to investigate effect on their sensitivity to conventional antibiotics. Methanol extracts from the root and stem‐bark of W. ugandensis were active against the test strains and their inhibitory effect was significantly different (p<0.05) from that of other extracts. We determined that the extracts had an inhibitory rather than a lytic (cidal) mode of action. The extracts from this plant had an effective MIC of 42 μg/ml and exhibited an inhibitory mode of action and did not elicit resistance to conventional antibiotics. Methanol extracts from the root and bark of this plant may provide potential sources for further development of alternative antimicrobial agents for the treatment of MDR infections.Key words: multi‐drug resistant (MDR) bacteria, minimum inhibitory concentrations (MICs), sub‐lethal concentration (SLC

Laboratory surveillance of cholera in Nyanza province during the outbreak from April to July 2007

Cholera continues to be an important cause of morbidity and mortality in many areas of the world, and there is currently a high frequency of new outbreaks in Africa. Following a confirmed cholera outbreak in Siaya, Kisumu, Bondo and Nyando districts, Nyanza province in western Kenya between April and July 2007, a laboratory surveillance study was conducted at the New Nyanza Provincial General Hospital’s Microbiology Laboratory. The study aimed at isolating and identifying the strain of Vibrio cholerae, Identifying the mean age of the patients and monitoring the susceptibility patterns to major antibiotics. It further aimed at determining effectiveness of empiric management of cholera. A total of 219 samples were processed out of which a total of 85 samples (39%) were found positive for Vibrio cholerae 01 sero‐type Inaba. The mean age recorded was 19 years (1 ≤ 80). The modal ages recorded were 8, 20 and 25. 55% (47) of the recorded cases were females while 45% (38) were males. Generally, V.Cholerae 01 sero‐type Inaba showed antibiotic resistance to trimethoprim‐sulfamethoxazole, nalidixic acid, sulfasoxazole, streptomycin and furazolidone. Tetracycline a commonly used antibiotic for empiric management was 100% effective on all isolates and remains the drug of choice. Samples obtained for case‐control study did not yield any cholera isolate. No prior exposure to any antibiotic was recorded among all the subjects. The study confirmed the effectiveness of empirical therapy on cholera and further identified the need of proper hygiene, water treatment, proper waste management and proper eating habits as means of controlling morbidity and mortality of cholera.Key words: Cholera, morbidity, mortality, empiric management, antibiotic resistanc

Evaluation of an Antigen-Antibody “Combination” Enzyme Linked Immunosorbent Assay for Diagnosis of Hepatitis C Virus Infections

Background: Development of “combination” assays detecting in parallel, within a single test, Hepatitis C Virus (HCV) antigens and antibodies, not only reduces the window period in HCV-infection but also costs. Reduction of costs is important for developing countries where money and personal resources are limited.Methods: We compared the Monolisa® HCV Antigen-Antibody Ultra (Bio-Rad Laboratories Limited, Marnes La Coquette, France) with the AXSYM HCV version 3.0 (Abbot Diagnostics, Germany)-the latter assay detecting only antibodies to HCV. Seventy three HCV-PCR positive and negative samples were tested.Results: Although the two assays showed comparable results, two samples from a bone marrow transplant (BMT) patient of viral loads 7.8 x 105 and 8.9 x 106 IU/mL could not be detected by the Monolisa® HCV Antigen-Antibody Ultra assay. Failure to detect the two samples with viral loads considered above threshold of detection for antigen proteins suggested a lack of sensitivity by this assay to discover viral capsid protein in patient samples. Genotyping of these samples revealed genotype 1b, a HCV-subtype which is widespread and should thus be easily detected.Conclusion: We conclude that although this assay depicts high sensitivity and specificity in detecting antibodies to HCV, it seems not to add further benefit in our study population to detect HCV infections by enhanced sensitivity due the potential contingency to trace viral capsid antigens.Keywords: Ag-Ab Combination assay, Hepatitis C Virus, ELISA, Monolisa HCV Ag-Ab Ultr

Left High and Dry: Deglaciation of Dogger Bank, North Sea, Recorded in Proglacial Lake Evolution

Reconstructions of palaeo-ice sheet retreat in response to climate warming using offshore archives can provide vital analogs for future ice-sheet behavior. At the Last Glacial Maximum, Dogger Bank, in the southern North Sea, was covered by the Eurasian Ice Sheet. However, the maximum extent and behavior of the ice sheet in the North Sea basin is poorly constrained. We reveal ice-marginal dynamics and maximum ice extent at Dogger Bank through sedimentological and stratigraphic investigation of glacial and proglacial lake sediments. We use a large, integrated subsurface dataset of shallow seismic reflection and geotechnical data collected during windfarm site investigation. For the first time, an ice stream is identified at Dogger Bank, based on preserved subglacial bedforms, eskers and meltwater channels. During ice-sheet advance, a terminal thrust-block moraine complex formed, whose crest runs approximately north-northeast to south-southwest. Subsequent ice stream shutdown caused stagnation of ice, and rapid retreat of the ice-sheet margin. The moraine complex, and outwash head from an adjacent ice-sheet lobe to the west, dammed a large (approximately 750 km2) proglacial lake. Subsequent sedimentation infilled the lake with 30 m of glacial outwash sediments. A lobate subaqueous fan formed at the ice-sheet margin, which thins toward the southeast with iceberg scours and ice-rafted debris at the base, and is onlapped by lake sediments calibrated to core as alternating clay and silt laminae, interpreted to be varves. The lake became isolated from the retreating ice-sheet margin, and ice-sheet retreat slowed. Sediment-laden meltwater was supplied to the ice-distal proglacial lake for c. 1500–2000 years. Subsequent ice-sheet retreat off Dogger Bank was more rapid due to the negative subglacial slope. The stepped retreat of rapid downwasting, slow retreat, and a final rapid phase off Dogger Bank occurred after the LGM at around 27 ka and before formation of a ribbon lake, dated previously to 23 ka and approximately 60 m lower in elevation, formed to the north of Dogger Bank. The complicated stratigraphic architecture revealed through these data improves forecasting of ground conditions for turbine footings at Dogger Bank, an important step in the provision of clean, sustainable energy

Topographic and hydrodynamic controls on barrier retreat and preservation: An example from Dogger Bank, North Sea

Barrier retreat can occur due to in-place drowning, overstepping or rollover, depending on the interplay of controls such as sea-level rise, sediment supply, coastal hydrodynamic regime and topography. Offshore sedimentary archives of barriers active during rapid Holocene sea-level rise provide important records of marine transgression, which are vital analogues to support appropriate mitigation strategies for future coastal realignment under projected relative sea-level rise scenarios. This study analyses the sedimentary archive at Dogger Bank, which is a formerly-glaciated area in the North Sea. Dogger Bank experienced marine transgression due to Early Holocene rapid relative sea-level rise. An integrated dataset of vibrocores and high-resolution seismic reflection data permits a stratigraphic framework to be established, which reveals the buried coastal geomorphology of the southern Dogger Bank for the first time. A transgressive stratigraphy was identified, comprising a topographically complicated basal glacial and terrestrial succession, overlain by two phases of barrier and tidal mudflat deposition, prior to shallow marine sedimentation. Barrier phase A was a recurved barrier drowned in place, and discontinuously overstepped to barrier phase B, which experienced continuous overstepping. By linking barrier elevations to relative sea-level curves, the timing of each barrier phase was established. Both barrier phases retreated during periods of rapid sea-level rise with abundant sediment supply. Coastal hydrodynamics (increasing wave energy) and antecedent topography with spatially variable accommodation are suggested to be the main reason for differing retreat mechanisms, rather than the rate of sea-level rise. Antecedent coastal geomorphology plays a critical role in erosional and depositional patterns during transgression, and therefore on the timing, rate and location of marine inundation, which needs to be included in models that aim to forecast hazards in coastal areas

DAYA ANTIBAKTERIAL EKSTRAK UMBI WORTEL (Daucus carrota) TERHADAP Escherichia coli SECARA IN VITRO

Tujuan penelitian ini untuk mengetahui daya antibakterial ekstrak umbi wortel dan besarnya konsentrasi ekstrak umbi wortel sebagai antibakterial terhadap Escherichia coli

Bruner’s cognitive stages and their effects on the understanding of fraction concept

This study aimed at determining: (1) the difference in the effect of employing the Bruner’s cognitive stages on the students’ understanding of fractions concept, (2) which of the Bruner’s cognitive stages gave better effect on the students’ understanding of the fractions concept, and (3) the linkage of Bruner’s cognitive stages which gave the best effect on the students' understanding of fractions concept based on the students’ self-efficacy. This research was a mixed method of the explanatory sequential type that combined quantitative and qualitative research. At first, experimental research was conducted then a case study was conducted to complement quantitative data. The population of this study was students of grade VII of SMP Negeri 2 Semarapura consisted of nine classes and the samples were three classes chosen with random sampling technique. The data of students' understanding of fractions concept were collected through the test and students’ self-efficacy data were collected using questionnaires, observation, and interviews

PENGARUH IRADIASI SINAK·X TERHADAP LEKOSIT MENCIT

Pengaruh iradiasi sinar-X terhadap lekosit mencit. Salah satu sindrom biologi akibat radiasi adalah sindrom hemopoetik