Insulation assembly uses cryopumping to reduce heat transfer in cryogenic liquid line

Flush joint coupling using foil jacketed annular cone, backfilled with carbon dioxide, eliminates inherent connection problems of conventional bayonet type joints used for vacuum jacketed assemblies

Vacuum-jacketed rotary joints for pipelines

Joint absorbs all significant torque, is as rugged as heavy metallic pipe, and has thermal insulation comparable to length of flexible pipe. Design is adaptable to any pipes transporting gases or liquids, including those which are noxious or corrosive

Vacuum jacketed umbilical lines technology advancement study. Task 4 - Rotary joint Final technical report

Vacuum jacketed components on launch umbilical tower service arms at Launch Complex 39 - low heat cryogenic rotary join

Vacuum jacketed umbilical lines technology advancement study. Task 7 - Bayonet joint Final technical report

Vacuum jacketed components on launch umbilical tower service arms at Launch Complex 39 - bayonet joints in cryogenic propellant and vent line

Modular pathways for editing non-cognate amino acids by human cytoplasmic leucyl-tRNA synthetase

To prevent potential errors in protein synthesis, some aminoacyl-transfer RNA (tRNA) synthetases have evolved editing mechanisms to hydrolyze misactivated amino acids (pre-transfer editing) or misacylated tRNAs (post-transfer editing). Class Ia leucyl-tRNA synthetase (LeuRS) may misactivate various natural and non-protein amino acids and then mischarge tRNALeu. It is known that the fidelity of prokaryotic LeuRS depends on multiple editing pathways to clear the incorrect intermediates and products in the every step of aminoacylation reaction. Here, we obtained human cytoplasmic LeuRS (hcLeuRS) and tRNALeu (hctRNALeu) with high activity from Escherichia coli overproducing strains to study the synthetic and editing properties of the enzyme. We revealed that hcLeuRS could adjust its editing strategy against different non-cognate amino acids. HcLeuRS edits norvaline predominantly by post-transfer editing; however, it uses mainly pre-transfer editing to edit α-amino butyrate, although both amino acids can be charged to tRNALeu. Post-transfer editing as a final checkpoint of the reaction was very important to prevent mis-incorporation in vitro. These results provide insight into the modular editing pathways created to prevent genetic code ambiguity by evolution

An Expanded Set of Amino Acid Analogs for the Ribosomal Translation of Unnatural Peptides

BACKGROUND: The application of in vitro translation to the synthesis of unnatural peptides may allow the production of extremely large libraries of highly modified peptides, which are a potential source of lead compounds in the search for new pharmaceutical agents. The specificity of the translation apparatus, however, limits the diversity of unnatural amino acids that can be incorporated into peptides by ribosomal translation. We have previously shown that over 90 unnatural amino acids can be enzymatically loaded onto tRNA. METHODOLOGY/PRINCIPAL FINDINGS: We have now used a competition assay to assess the efficiency of tRNA-aminoacylation of these analogs. We have also used a series of peptide translation assays to measure the efficiency with which these analogs are incorporated into peptides. The translation apparatus tolerates most side chain derivatives, a few alpha,alpha disubstituted, N-methyl and alpha-hydroxy derivatives, but no beta-amino acids. We show that over 50 unnatural amino acids can be incorporated into peptides by ribosomal translation. Using a set of analogs that are efficiently charged and translated we were able to prepare individual peptides containing up to 13 different unnatural amino acids. CONCLUSIONS/SIGNIFICANCE: Our results demonstrate that a diverse array of unnatural building blocks can be translationally incorporated into peptides. These building blocks provide new opportunities for in vitro selections with highly modified drug-like peptides

Leben Friedrich des Zweiten, Königs von Preussen [et]c. : Für den Bürger und Bauernstand bearbeitet

von Friedrich Samuel MursinnaAutopsie nach Ex. der ULB Sachsen-AnhaltVorlageform des Erscheinungsvermerks: Halle, In Kommißion bei J. Fr. Dost. 1794

Abhandlung von den Krankheiten der Schwangern, Gebärenden, Wöchnerinnen und Säuglinge

Von Christian Ludwig Mursinna, dritten Generalchirurgus, erstem Professor der Chirurgie bei dem Collegio Medico-Chirurgico ... erstem Geburtshelfer in der Charitee.Vorlageform der Veröffentlichungsangabe: Berlin, ... Bei Christian Friedrich Himbur