Attitudes Towards Age-Appropriate Breastfeeding

The advantages of breastfeeding have been clearly outlined by a variety of research studies, and the World Health Organization clearly states that the “appropriate duration for breastfeeding is six months of exclusive breastfeeding and then breastfeeding with supplements until two years” (WHO, 2010). Opinions and attitudes towards the appropriate breastfeeding duration have not been adequately researched, which could have implications as to why mothers are not breastfeeding according to the WHO’s standards. This study focused on age-appropriate attitudes towards infant feeding practices. Three hundred and ninety-one students completed an online survey for their undergraduate psychology 101 class. The first hypothesis proposed that participants who were are parents would display greater comfort with breastfeeding at each time point (9, 12, and 15 months) compared to non-parents, and the data supported this hypothesis. The second hypothesis suggested that participants who had more positive attitudes towards breastfeeding would be more comfortable seeing a baby being breastfed in front of them at when a child was 9, 12 and 15 months. A correlation analysis supported this hypothesis. The third hypothesis predicted a positive correlation between age and comfort with breastfeeding at 9, 12 and 15 months. A correlation analysis showed older students were more comfortable with seeing older infants being breastfed than younger students. The fourth hypothesis proposed that participants who are more comfortable with public displays of breastfeeding would be more positive towards breastfeeding older children. The data supported this hypothesis

Measuring Endocytosis During Proliferative Cell Quiescence

Quiescence (also called "G0") is the state in which cells have exited the cell cycle but are capable to reenter as required. Though poorly understood, it represents one of the most prevalent cell states across all life. Many biologically important cell types reside in quiescence including mature hepatocytes, endothelial cells, and dormant adult stem cells. Furthermore, the quiescence program occurs in both short- and long-term varieties, depending on the physiological environments. A barrier slowing our understanding of quiescence has been a scarcity of available in vitro model systems to allow for the exploration of key regulatory pathways, such as endocytosis. Endocytosis, the internalization of extracellular material into the cell, is a fundamental and highly regulated process that impacts many cell biological functions. Accordingly, we have developed an in vitro model of deep quiescence in hTERT-immortalized RPE1 cells, combining both long-term contact inhibition and mitogen removal, to measure endocytosis. In addition, we present an analytical approach employing automated high-throughput microscopy and image analysis that yields high-content data allowing for meaningful and statistically robust interpretation. Importantly, the methods presented herein provide a suitable platform that can be easily adapted to investigate other regulatory processes across the cell cycle

Influence of Vineyard Vegetational Borders on Western Grape Leafhopper (Erythroneura elegantula Osborn), its Egg Parasitoids (Anagrus spp.) and Generalist Insect Predators

Studies have shown that vegetational diversity in or around cropping systems can enhance naturalenemy abundance, although the impact on herbivores is less certain. We studied the influence of vineyardvegetational borders on density of the western grape leafhopper, Erythroneura elegantula, its majorparasitoids, Anagrus spp., and two generalist predators, Orius spp. and Leptothrips mali. Two studyvineyards had planted, perennial flowering hedgerows, one bordered a natural riparian zone, and onehad a sparse border of native trees. From April to September 2007, we counted leafhopper nymphs andadults of Anagrus spp., Orius spp. and L. mali within 10 m of the border, and at additional 20 m intervalsup to 90 m. At two sites with a diverse border, leafhopper density was stable nearest the border; at otherdistances leafhopper density decreased between the first and second generations. This effect was notseen at the sparse border site. Anagrus spp. density was enhanced at one site with a diverse border, butonly late season. There was no border effect on the generalist insect predators, by site or early vs. lateseason. Regression analysis showed a positive relationship between leafhopper and Anagrus spp. density,suggesting that the parasitoids were responding to higher leafhopper density. We conclude that, dependingon the nature of the border vegetation, there can be an effect on leafhopper nymphal density, but in thisstudy there is no evidence that it was due to natural enemies

Taking your participants home: Self care within the research process

Background: On the long and often stressful road to qualifying as a Counselling Psychologist the issue of self care is addressed early within the training. Yet this idea of self care relates to therapeutic practice, with little consideration of self care during the research process. With the training route now at Doctoral level, all practitioners will undertake a significant research project as part of the qualifying portfolio. Aims and Scope: This paper explores the process issues encountered in conducting research into the experience of Childhood Sexual Abuse (CSA), and raises questions about the way self-care is addressed within the research process. The article reflects upon a Content Analysis of 202 personal accounts of CSA with the aim of exploring the presence of Stockholm syndrome and PTSD within CSA. Literature from self care within the clinical application of psychology and trauma-based work is discussed as a starting point for the development of self care within a research context. Conclusion: Recommendations are made for changes in research practice and ethical review, and a clear role for Counselling Psychologists to lead in this development

Cell-fate determination by ubiquitin-dependent regulation of translation.

Metazoan development depends on the accurate execution of differentiation programs that allow pluripotent stem cells to adopt specific fates. Differentiation requires changes to chromatin architecture and transcriptional networks, yet whether other regulatory events support cell-fate determination is less well understood. Here we identify the ubiquitin ligase CUL3 in complex with its vertebrate-specific substrate adaptor KBTBD8 (CUL3(KBTBD8)) as an essential regulator of human and Xenopus tropicalis neural crest specification. CUL3(KBTBD8) monoubiquitylates NOLC1 and its paralogue TCOF1, the mutation of which underlies the neurocristopathy Treacher Collins syndrome. Ubiquitylation drives formation of a TCOF1-NOLC1 platform that connects RNA polymerase I with ribosome modification enzymes and remodels the translational program of differentiating cells in favour of neural crest specification. We conclude that ubiquitin-dependent regulation of translation is an important feature of cell-fate determination

Encouraging children to think counterfactually enhances blocking in a causal learning task

According to a higher order reasoning account, inferential reasoning processes underpin the widely observed cue competition effect of blocking in causal learning. The inference required for blocking has been described as modus tollens (if p then q, not q therefore not p). Young children are known to have difficulties with this type of inference, but research with adults suggests that this inference is easier if participants think counterfactually. In this study, 100 children (51 five-year-olds and 49 six- to seven-year-olds) were assigned to two types of pretraining groups. The counterfactual group observed demonstrations of cues paired with outcomes and answered questions about what the outcome would have been if the causal status of cues had been different, whereas the factual group answered factual questions about the same demonstrations. Children then completed a causal learning task. Counterfactual pretraining enhanced levels of blocking as well as modus tollens reasoning but only for the younger children. These findings provide new evidence for an important role for inferential reasoning in causal learning