Managing scientific data for long-term access and use

Preservation of data for long-term use will require data management strategies that include curation and preservation planning and implementation. While data management and curatorial activities have been an integral part of some scientific domains for years (see for example, high energy particle physics), these are new concepts in other areas of science. Concepts such as provenance, representation for re-use, and work-flow capture are rarely understood, let alone addressed. By bringing together theories and best practices from archives, museum studies, and library and information science (LIS), it is possible to address these problems. on current research into scientific data management problems, this panel will consider questions about sharing and re-use of data, curation and preservation, and the intersection of scientific production and scholarly communication. Our research explores information work and problems across a range of scientific areas in the life and physical sciences, including genomics, neuroscience, ecology, and earth science. As more scientific work products are shifted to open or shared data collections (including archives, repositories and databases), we will need to understand how these systems are implemented and used to support collaboration and discovery, as well as scholarly and scientific communication.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/57315/1/14504301123_ftp.pd

Reflectance confocal microscopy for the diagnosis of skin infections and infestations

Reflectance confocal microscopy (RCM) is a noninvasive real-time imaging technique that has been widely used for the diagnosis of skin cancer. More recently, it has been reported as a useful tool for the diagnosis and management of several inflammatory and infectious skin disorders. This article provides an overview of the current available applications of RCM use in cutaneous infections and infestations. PubMed was used to search the following terms in various combinations: reflectance confocal microscopy, skin, hair, nail, infection, parasitosis, mycosis, virus, bacteria. All papers were accordingly reviewed. In most cutaneous infections or infestations, the main alterations are found in the epidermis and upper dermis, where the accuracy of confocal microscopy is nearly similar to that of histopathology. The high resolution of this technique allows the visualization of most skin parasites, fungi, and a few bacteria. Although viruses cannot be identified because of their small size, viral cytopathic effects can be observed on keratinocytes. In addition, RCM can be used to monitor the response to treatment, thereby reducing unnecessary treatments

Innovative Educational Design: The Development of Autonomous Schools

Innovative Educational Design: The Development of Autonomous Schools captures an educator’s journey in developing an economically integrated, urban Expeditionary Learning charter school, an autoethnography of an educator embracing an entrepreneurial spirit and traversing the years prior to opening a school and obtaining unanimous approval from the authorizing board of education. Autoethnography provides the researcher with an opportunity to turn scholarly interests inward, bringing personal experiences to center stage revealing the culture of founding school leaders. Similar to an ethnographer describing the culture of a group of people and learning what it is like to be a part of the group from the viewpoint of members of that group, the researcher describes experiences designing an autonomous Expeditionary Learning charter school in an urban environment in the Rocky Mountain region. Autoethnography adheres to the anthropological and social approaches to scientific inquiry, providing opportunities for the autoethnographer to reflect upon, analyze, and interpret story within the broader sociocultural context. Personal memory data, self-reflective data, and external data were utilized to explore the aspects of the researcher’s experiences developing an Expeditionary Learning charter school that would best support future education entrepreneurs in designing autonomous schools. An additional purpose of this autoethnographic study is to illuminate the problems and possibilities for preparing effective school leaders and providing early career support to nurture their professional development. With insights into the researcher’s experiences founding a downtown school, the author identifies important aspects of school design, including school culture, leadership, educational program, teaching, and governance and reveals the hidden competencies school leaders need to possess to succeed in an increasingly changing educational landscape. The author hopes that this autoethnography will reveal the complexities facing autonomous school leaders as they navigate state and district level authorization processes and, in the process, provide an opportunity for new knowledge, insight, and transformation for the author, the reader, and the field of choice and innovation in education

Desmoplastic Trichoepithelioma and Melanocytic Nevus: Dermoscopic and Reflectance Confocal Microscopy Presentation of a Rare Collision Tumor

A 27-year-old woman presented with a 2-year history of an asymptomatic papule located on her right cheek. The physical examination revealed a firm, well-defined with a raised annular border, skin-colored papule, 5 mm in maximum diameter

Verrucous Melanoma Simulating Melanoacanthoma: Dermoscopic, Reflectance Confocal Microscopic and High-Definition Optical Coherence Tomography Presentation of a Rare Melanoma Variant

info:eu-repo/semantics/publishedVersio

Dermoscopic and Reflectance Confocal Microscopic Presentation of Hailey‐Hailey Disease: a Case Series

Background/purpose: Hailey-Hailey disease is a rare inherited acantholytic skin disorder characterized by heterogeneous clinical presentation. Its differential diagnosis might be wide, including other genodermatoses, inflammatory, and infectious skin diseases. Although histopathology remains as diagnostic gold standard, noninvasive techniques such as dermoscopy and reflectance confocal microscopy may assist clinical examination. Herein, we aim to further characterize the dermoscopic and reflectance confocal microscopic presentation of Hailey-Hailey disease with histologic correlation. Methods: Eight patients with Hailey-Hailey disease were consecutively recruited. All patients were examined using dermoscopy and reflectance confocal microscopy. Results: In all cases, dermoscopy enabled the visualization of polymorphous vessels, including glomerular and linear-looped vessels, within a pink-whitish background. Reflectance confocal microscopy revealed wide suprabasilar partial acantholysis and clefting, crusts, dilated papillae with tortuous vessels, and inflammatory cells. Dyskeratosis, uplocated papillae, and adnexal sparing were also observed. Conclusion: Although definite diagnosis was obtained by histopathology in all cases, dermoscopy and reflectance confocal microscopy allowed the identification of common features (even in cases with dissimilar clinical presentation) that may support an early diagnosis of Hailey-Hailey disease, and its differentiation from other more frequent skin disorders.info:eu-repo/semantics/publishedVersio

Cortical circuit alterations precede motor impairments in Huntington's disease mice

Huntington's disease (HD) is a devastating hereditary movement disorder, characterized by degeneration of neurons in the striatum and cortex. Studies in human patients and mouse HD models suggest that disturbances of neuronal function in the neocortex play an important role in disease onset and progression. However, the precise nature and time course of cortical alterations in HD have remained elusive. Here, we use chronic in vivo two-photon calcium imaging to longitudinally monitor the activity of identified single neurons in layer 2/3 of the primary motor cortex in awake, behaving R6/2 transgenic HD mice and wildtype littermates. R6/2 mice show age-dependent changes in cortical network function, with an increase in activity that affects a large fraction of cells and occurs rather abruptly within one week, preceeding the onset of motor defects. Furthermore, quantitative proteomics demonstrate a pronounced downregulation of synaptic proteins in the cortex, and histological analyses in R6/2 mice and human HD autopsy cases reveal a reduction in perisomatic inhibitory synaptic contacts on layer 2/3 pyramidal cells. Taken together, our study provides a time-resolved description of cortical network dysfunction in behaving HD mice and points to disturbed excitation/inhibition balance as an important pathomechanism in HD

Apresentação Dermatoscópica e Microscópica Confocal de Melanoma Raro do Mamilo

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Long-term in vivo imaging of fibrillar tau in the retina of P301S transgenic mice.

Tauopathies are widespread neurodegenerative disorders characterised by the intracellular accumulation of hyperphosphorylated tau. Especially in Alzheimer's disease, pathological alterations in the retina are discussed as potential biomarkers to improve early diagnosis of the disease. Using mice expressing human mutant P301S tau, we demonstrate for the first time a straightforward optical approach for the in vivo detection of fibrillar tau in the retina. Longitudinal examinations of individual animals revealed the fate of single cells containing fibrillar tau and the progression of tau pathology over several months. This technique is most suitable to monitor therapeutic interventions aimed at reducing the accumulation of fibrillar tau. In order to evaluate if this approach can be translated to human diagnosis, we tried to detect fibrillar protein aggregates in the post-mortem retinas of patients that had suffered from Alzheimer's disease or Progressive Supranuclear Palsy. Even though we could detect hyperphosphorylated tau, we did not observe any fibrillar tau or Aß aggregates. In contradiction to previous studies, our observations do not support the notion that Aβ or tau in the retina are of diagnostic value in Alzheimer's disease