Efecto del tiempo de espera en el matadero sobre el bienestar de corderos lechales

In this paper, the effect of lairage before slaughter on the welfare and carcass and meat quality in suckling lambs has been studied. Four lairage times have been evaluated, L0 no-lairage, L3 lairage of 3 h, L6 lairage of 6 h and L12 lairage of 12 h. Blood parameters, cortisol, creatine kinase (CK), lactate dehydrogenase (LDH), total protein and amyloid A protein, weight loss during lairage, carcass quality and meat pH in two longissimus and semitendinosus muscles have been studied. Lambs with a lairage of 12 hours showed higher level of cortisol, total protein and amyloid A protein, besides a greater live weight loss, indicating that had a higher stress response and further dehydration. The meat pH was higher in both muscles in the group L3. The lairage increased animal welfare concerns but not the quality of the meat.En este trabajo se estudia el efecto que el tiempo de espera antes del sacrificio tiene sobre el bienestar y la calidad de la canal y la carne en corderos lechales. Se han estudiado cuatro tiempos de espera, L0 sin periodo de espera, L3 espera de 3 h, L6 espera de 6 h y L12 espera de 12 h. Se han valorado parámetros sanguíneos, cortisol, creatin kinasa (CK), lactato deshidrogenasa (LDH), proteínas totales y proteína amiloide A, así como pérdidas de peso por la espera, calidad de la canal y la evolución del pH de la carne en dos músculos longissimus y semitendinosus. Los corderos que estuvieron esperando 12 h mostraron un mayor nivel de cortisol, proteínas totales y proteína amiloide A, además de tener una mayor pérdida de peso, lo que indica que tuvieron una mayor respuesta de estrés y mayor deshidratación. El pH de la carne fue más alto en los dos músculos para el grupo que estuvo esperando 3 h. El incremento de tiempo de espera afecta al bienestar del animal pero no a la calidad de la carn

Efecto de los grados día de ayuno y del hacinamiento previos al sacrificio sobre el contenido estomacal y respuesta de estrés en trucha arcoíris (Oncorhynchus mykiss)

El ayuno pre-sacrificio es una práctica rutinaria en acuicultura que se lleva a cabo para vaciar el aparato digestivo disminuyendo la cantidad de heces y previniendo una contaminación de la canal (Robb, 2008). Sin embargo, el ayuno puede también incrementar los niveles de estrés de los peces y si estos son lo suficientemente altos afectan a la calidad de la canal (Poli et al., 2005)

Immediate effects of a telerehabilitation program based on aerobic exercise in women with fibromyalgia

Background: We analyzed the immediate effects of a Telerehabilitation Program (TP) based on aerobic exercise in women with fibromyalgia (FM) syndrome during the lockdown declared in Spain due to the COVID-19 pandemic. Methods: A single-blind randomized controlled trial was designed. Thirty-four women with FM were randomized into two groups: TP group and Control group. The intervention lasted 15 weeks, with 2 sessions per week. The TP based on aerobic exercise was guided by video and the intensity of each session was monitored using the Borg scale. Pain intensity (Visual Analogue Scale), mechanical pain sensitivity (algometer), number of tender points, FM impact (Revised Fibromyalgia Impact Questionnaire), pain catastrophizing (Pain Catastrophizing Scale), physiological distress (Hospital Anxiety and Depression Scale), upper (Arm Curl Test) and lower-limb physical function (6-min Walk Test) were measured at baseline and after the intervention. Results: The TP group improved pain intensity (p = 0.022), mechanical pain sensitivity (p 0.05). Conclusion: A TP based on aerobic exercise achieved improvements on pain intensity, mechanical pain sensitivity, and psychological distress compared to a Control group during the lockdown declared in Spain due to COVID-19 pandemic

Bienestar animal de corderos ternascos durante su espera en matadero

El presente trabajo consistió en valorar la respuesta de estrés al que son sometidos los corderos desde que llegan al matadero hasta su sacrificio en distintas épocas del año, primavera y verano. Se tomaron muestras de sangre durante el sacrificio del animal para la valoración de diferentes parámetros sanguíneos de respuesta de estrés y tras el faenado se valoró la calidad de la canal y de la carne de los corderos. Se encontraron diferencias significativas con mayores valores para los corderos sacrificados en verano en el número de linfocitos, lactato deshidrogenasa (LDH), cortisol y pHs y menores en el número de leucocitos, neutrófilos, relación neutrófilos/linfocitos, volumen corpuscular medio, concentración de glucosa y rendimiento de la canal. Los corderos sacrificados en verano tuvieron más comprometido su nivel de bienestar por el mayor nivel de cortisol y LDH junto con unos pHs más elevados, lo que hace que la calidad de la carne sea inferior a los sacrificados en primavera

Efecto de la suplementación con antioxidantes en la dieta de corderos sobre la calidad de su carne enriquecida en ácidos grasos omega-3

Se ha evaluado el efecto antioxidante de la suplementación en la dieta de corderos con vitamina E o con extracto de uva rico en polifenoles en carne enriquecida en ácidos grasos omega-3 mediante la alimentación y conservada durante 0, 6 y 12 días en atmósfera modificada. Se observó una interacción significativa (p<0.001) entre el tipo de suplementación y el tiempo de conservación para los valores de oxidación lipídica y de proporción de metamioglobina, mostrando la carne suplementada con vitamina E los valores más bajos al final de la conservación. Los niveles de ácidos grasos poliinsaturados descendieron durante la conservación en el grupo control y en el grupo suplementado con extracto de uva, permaneciendo más estables en el grupo suplementado con vitamina E. Se observó una interacción significativa (p<0.001) entre la suplementación y el tiempo de conservación para el porcentaje de ácidos grasos omega- 3, manteniéndose los valores estables durante la conservación en la carne suplementada con vitamina E. La suplementación en la dieta con vitamina E mostró un efecto antioxidante en la carne durante su conservación mayor que el resto de dietas estudiadas

Efecto de la adicción de extracto de vino tinto en la conservación de hamburguesas de cordero enriquecidas en ácidos grasos omega-3

Red wine is a great source of polyphenols compounds, which exert a high antioxidant capacity. The effect of red wine extract (EV) on the oxidative stability of lamb patties in terms of metmyoglobin (MetMb) formation, lipid oxidation, protein oxidation, and the stability of docosahexaenoic fatty acid (DHA) was investigated. Ground lamb meat enriched in ω3 fatty acids was divided into four treatments. Three treatments were supplemented with 3 doses of EV being 50 (EV1), 100 (EV2) and 200 (EV3) mg GAE/kg meat and, the last one, without antioxidant supplementation, was kept as control (C). The lamb patties were stored under MAP (70% O2/30% CO2) during 9 days. There was an interaction between treatment (L) and storage period (PC) for MetMb proportion (p<0.001) and DHA content (p<0.01). Groups EV2 and EV3 showed less MetMb proportion and higher DHA content at the end of storage period in comparison to groups EV1 and C. Lipid and protein oxidation was affected by storage period (p<0.001), increasing in all treatments.El vino tinto es una gran fuente de compuestos polifenólicos que presentan una gran capacidad antioxidante. El objetivo de este estudio fue evaluar el efecto de la adición de extracto de vino (EV) en la estabilidad oxidativa de hamburguesas de cordero, estudiando la formación de metamioglobina (MetMb), la oxidación lipídica, la oxidación proteica y el contenido en ácido docosahexaenoico (DHA). Se realizaron 4 lotes de carne picada de cordero, todos enriquecidos en ácidos grasos ω3. A tres lotes se les adicionó EV para obtener una concentración final de 50 (EV1), 100 (EV2) y 200 (EV3) mg GAE/kg de carne y el último lote, sin antioxidantes, se mantuvo como control (C). Las hamburguesas se conservaron en MAP (70% O2/30% CO2) durante 9 días. Se observó una interacción entre el lote (L) y el periodo de conservación (PC) para la proporción de MetMb (p<0,001) y el contenido en DHA (p<0,01). Los lotes EV2 y EV3 presentaron menor proporción de MetMb y mayor contenido en DHA que los lotes EV1 y C. La oxidación lipídica y proteica estuvo afectada por el periodo de conservación (p<0,001) aumentando en todos los tratamientos

Association of a single nucleotide polymorphism combination pattern of the Klotho gene with non-cardiovascular death in patients with chronic kidney disease

Chronic kidney disease (CKD) is associated with an elevated risk of all-cause mortality, with cardiovascular death being extensively investigated. However, non-cardiovascular mortality represents the biggest percentage, showing an evident increase in recent years. Klotho is a gene highly expressed in the kidney, with a clear influence on lifespan. Low levels of Klotho have been linked to CKD progression and adverse outcomes. Single nucleotide polymorphisms (SNPs) of the Klotho gene have been associated with several diseases, but studies investigating the association of Klotho SNPs with noncardiovascular death in CKD populations are lacking. The main aim of this study was to assess whether 11 Klotho SNPs were associated with non-cardiovascular death in a subpopulation of the National Observatory of Atherosclerosis in Nephrology (NEFRONA) study (n ¼ 2185 CKD patients). After 48 months of follow-up, 62 cardiovascular deaths and 108 non-cardiovascular deaths were recorded. We identified a high non-cardiovascular death risk combination of SNPs corresponding to individuals carrying the most frequent allele (G) at rs562020, the rare allele (C) at rs2283368 and homozygotes for the rare allele (G) at rs2320762 (rs562020 GG/AG þ rs2283368 CC/CT þ rs2320762 GG). Among the patients with the three SNPs genotyped (n ¼ 1016), 75 (7.4%) showed this combination. Furthermore, 95 (9.3%) patients showed a low-risk combination carrying all the opposite genotypes (rs562020 AA þ rs2283368 TT þ rs2320762 GT/TT). All the other combinations [n ¼ 846 (83.3%)] were considered as normal risk. Using competing risk regression analysis, we confirmed that the proposed combinations are independently associated with a higher fhazard ratio [HR] 3.28 [confidence interval (CI) 1.51-7.12]g and lower [HR 6 × 10- (95% CI 3.3 × 10--1.1 × 10-)] risk of suffering a non-cardiovascular death in the CKD population of the NEFRONA cohort compared with patients with the normal-risk combination. Determination of three SNPs of the Klotho gene could help in the prediction of non-cardiovascular death in CKD

Association of candidate gene polymorphisms with chronic kidney disease : Results of a case-control analysis in the NEFRONA cohort

Chronic kidney disease (CKD) is a major risk factor for end-stage renal disease, cardiovascular disease and premature death. Despite classical clinical risk factors for CKD and some genetic risk factors have been identified, the residual risk observed in prediction models is still high. Therefore, new risk factors need to be identified in order to better predict the risk of CKD in the population. Here, we analyzed the genetic association of 79 SNPs of proteins associated with mineral metabolism disturbances with CKD in a cohort that includes 2,445 CKD cases and 559 controls. Genotyping was performed with matrix assisted laser desorption ionization-time of flight mass spectrometry. We used logistic regression models considering different genetic inheritance models to assess the association of the SNPs with the prevalence of CKD, adjusting for known risk factors. Eight SNPs (rs1126616, rs35068180, rs2238135, rs1800247, rs385564, rs4236, rs2248359, and rs1564858) were associated with CKD even after adjusting by sex, age and race. A model containing five of these SNPs (rs1126616, rs35068180, rs1800247, rs4236, and rs2248359), diabetes and hypertension showed better performance than models considering only clinical risk factors, significantly increasing the area under the curve of the model without polymorphisms. Furthermore, one of the SNPs (the rs2248359) showed an interaction with hypertension, being the risk genotype affecting only hypertensive patients. We conclude that 5 SNPs related to proteins implicated in mineral metabolism disturbances (Osteopontin, osteocalcin, matrix gla protein, matrix metalloprotease 3 and 24 hydroxylase) are associated to an increased risk of suffering CKD

Corrigendum: Azole-resistance in aspergillus terreusand related species: An emerging problem or a rare phenomenon? (Frontiers in Microbiology (2018) 9 (516) DOI: 10.3389/fmicb.2018.00516)

Raquel Sabino was not included as an author in the published article. The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated. © 2019 Zoran, Sartori, Sappl, Aigner, Sánchez-Reus, Rezusta, Chowdhary, Taj-Aldeen, Arendrup, Oliveri, Kontoyiannis, Alastruey-Izquierdo, Lagrou, Lo Cascio, Meis, Buzina, Farina, Drogari-Apiranthitou, Grancini, Tortorano, Willinger, Hamprecht, Johnson, Klingspor, Arsic-Arsenijevic, Cornely, Meletiadis, Prammer, Tullio, Vehreschild, Trovato, Lewis, Segal, Rath, Hamal, Rodriguez-Iglesias, Roilides, Arikan-Akdagli, Chakrabarti, Colombo, Fernández, Martin-Gomez, Badali, Petrikkos, Klimko, Heimann, Uzun, Roudbary, de la Fuente, Houbraken, Risslegger, Sabino, Lass-Flörl and Lackner