226 research outputs found

    MULHERES NA MINERAÇÃO: CARREIRA, EQUILÍBRIO TRABALHO-FAMÍLIA E DISCRIMINAÇÃO

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    Objetivo: Este artigo analisou a percepção de profissionais, mulheres e homens, acerca da condição da mulher no ambiente de trabalho em uma empresa multinacional do setor de mineração. Método / abordagem: Uma pesquisa de campo survey foi aplicada em uma multinacional do setor de mineração contemplando uma amostra de 262 empregados. Principais resultados: Os resultados apontam que os homens apresentaram maior tendência de concordância em relação às questões sobre a carreira da mulher, ou seja, claramente os profissionais do sexo masculino percebem menos as barreiras à carreira das mulheres do que elas próprias. Quando ao indicador Preconceito e Discriminação, observou-se diferença significativa de percepção entre o sexo feminino e o masculino, evidenciando uma percepção mais aguçada das mulheres quanto à ocorrência de situações de preconceito e discriminação na organização. No indicador Trabalho e Família, diferentemente do esperado, os dados apontam que não houve diferença significativa de percepção entre o sexo feminino e masculino. Contribuições metodológicas / sociais / gerenciais: A pesquisa confirma barreiras vivenciadas pelas mulheres no quesito ascensão e desenvolvimento de carreira e corrobora a existência de situações de preconceito em contextos marcados pela hegemonia do masculino.  Originalidade / relevância: A mulher na mineração foi abordada em apenas um estudo nacional e, ao trazer à tona esse debate, o artigo reforça a importância de se pensar na elaboração de políticas e práticas focadas nas necessidades da mulher para que elas possam obter iguais condições de trabalho, salário e ascensão nas organizações

    Ortho-surgical treatment in skeletal CLII patients with temporomandibular dysfunction: Case report / Tratamento orto-cirurgico em paciente CLII esquelética e com disfunção temporomandibular: Relato de caso

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    The study reports the ortho-surgical treatment of a patient with class II dentofacial deformity and temporomandibular dysfunction. Patient R.C, 29 years old, mixed race, female, sought the Orthodontics course at Christus University Center, complaining of orofacial pain and reporting other types of pain treatments, but without success. After facial analysis, intraoral and extraoral clinical examination, analysis of panoramic radiography and teleradiographs, it was concluded that in addition to temporomandibular dysfunction the patient had a class II deformity, with anteroposterior deficiency, and occlusal instability. The proposed treatment was ortho-surgical and evaluation of painful symptoms before and after surgery through the TMD Research Diagnostic Criteria questionnaire (RDC / TMD). The patient received pre-surgical orthodontic preparation and was then referred for surgery, in which Le Fort I osteotomy was performed in the maxilla with 3 mm advancement and bilateral sagittal osteotomy in mandibular branches for 5 mm mandibular advancement, in addition to mentoplasty. with a further 5 mm feed. Fixation was performed with the Dual Side 2.0mm System (screws and plates), followed by physical therapy, speech therapy and orthodontic finishing. The patient did not show an improvement in bone positioning, profile and face were more harmonic, without losing its aesthetic identity and there was a significant reduction in pain sensitivity previously presented

    Genotoxicity detected during cyanobacteria bloom in a water supply reservoir

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    The aquatic ecosystems are susceptible to cyanobacterial blooms due to the eutrophication of water bodies caused by human activities. In this study, phytoplankton and cyanotoxins analysis, as well as cellular and genetic biomarkers of toxicity (Allium cepa test - higher plant test system), were evaluated in water samples of Alagados Reservoir during a cyanobacterial bloom in South Brazil. The water samples were collected during the wet season at two sites in the Reservoir. Paralytic shellfish toxins (PSTs) were detected in both samples (sites 1 and 2); however, the levels of PSTs were higher in site 1. Gonyautoxin 2 was the major cyanotoxin found in the Reservoir. Both samples were able to induce cytotoxic effects (reduced Mitotic Index) and damage the genetic material (i.e., increased frequencies of chromosome aberration and micronuclei) of meristematic cells of A. cepa. The cellular and genetic damages were higher in the sample site 1, wherein high levels of PSTs were verified. Thus, our findings suggested that cyanotoxins-contaminated waters may damage the genetic material of living organisms, and therefore this group of contaminants should be assessed for their potential genotoxicity

    Estratégias práticas de ensino sobre insetos para alunos dos anos iniciais do ensino fundamental

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    Oportunizar um ensino de ciências mais prático e didático possível pode ser mais atrativo e facilitador para o aluno aprender. O artigo objetivou elaborar, desenvolver e validar uma estratégia didática sobre a temática inseto para anos iniciais do ensino fundamental, oportunizando a compreensão das características deste grupo animal; bem como esclarecer a acerca da importância dos insetos na manutenção do equilíbrio do meio. Estas foram desenvolvidas com 165 alunos do primeiro ao quinto ano do ensino fundamental de uma escola pública do município de Palmeira das Missões, RS. Os resultados provenientes de um questionário, foram submetidos à análise de conteúdo e categorização e evidenciaram sobre o que os alunos mais gostaram e aprenderam nas atividades. Os estudantes destacaram os conhecimentos aprendidos sobre as características que identificam os insetos (sejam, as partes do corpo, metamorfose, representantes ou exoesqueleto), seguido da experiência em observar as abelhas, a atividade envolvendo a polinização e o jogo desenvolvido ao ar livre. Os resultados evidenciam que as atividades desenvolvidas foram todas citadas, em maior ou menor quantidade, podendo-se inferir que a sequência didática foi importante e proveitosa, contando com o envolvimento dos alunos, proporcionando um processo de ensino aprendizagem eficaz e prazeroso.&nbsp

    Microbiological quality of minimally processed vegetables commercialized in Brasilia, DF, Brazil

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    This study evaluated the microbiological quality of minimally processed vegetables commercialized in the city of Brasilia, DF, Brazil. A total of 32 samples of different vegetables were purchased from 10 supermarkets. In most samples (78.1%) the populations of psychrotrophic bacteria had a high count ranging from 106 to 108 CFU/g. Thermotolerant coliforms were found in all samples, with populations higher than 102 MPN/g in 15 samples (46.9%). After molecular analyses, E. coli was identified in 16 samples (50.0%) and Salmonella spp. in 4 samples (12.5%). S. aureus was found in 14 samples (43.8%), with counts higher than 103 CFU/g in 4 samples (12.5%). The results obtained in this study showed that 16 samples (50%) were unfit for consumption according to Brazilian legislation. These results indicated the need of adoption of better hygienic practices in the production of minimally processed vegetables to improve quality and microbiological safety

    Identification of R-Spondin Gene Signature Predictive of Metastatic Progression in BRAFV600E-Positive Papillary Thyroid Cancer

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    Papillary thyroid carcinoma (PTC) is the most common malignancy of the thyroid gland and early stages are curable. However, a subset of PTCs shows an unusually aggressive phenotype with extensive lymph node metastasis and higher incidence of locoregional recurrence. In this study, we investigated a large cohort of PTC cases with an unusual aggressive phenotype using a high-throughput RNA sequencing (RNA-Seq) to identify differentially regulated genes associated with metastatic PTC. All metastatic PTC with mutated BRAF (V600E) but not BRAF wild-type expressed an up-regulation of R-Spondin Protein 4 (RSPO4) concomitant with an upregulation of genes involved in focal adhesion and cell-extracellular matrix signaling. Further immunohistochemistry validation confirmed the upregulation of these target genes in metastatic PTC cases. Preclinical studies using established PTC cell lines support that RSPO4 overexpression is associated with BRAF V600E mutation and is a critical upstream event that promote activation of kinases of focal adhesion signaling known to drive cancer cell locomotion and invasion. This finding opens up the potential of co-targeting B-Raf, RSPO and focal adhesion proteins as a pharmacological approach for aggressive BRAF V600E PTC

    New Insights in the Sugarcane Transcriptome Responding to Drought Stress as Revealed by Supersage

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    In the scope of the present work, four SuperSAGE libraries have been generated, using bulked root tissues from four drought-tolerant accessions as compared with four bulked sensitive genotypes, aiming to generate a panel of differentially expressed stress-responsive genes. Both groups were submitted to 24 hours of water deficit stress. The SuperSAGE libraries produced 8,787,315 tags (26 bp) that, after exclusion of singlets, allowed the identification of 205,975 unitags. Most relevant BlastN matches comprised 567,420 tags, regarding 75,404 unitags with 164,860 different ESTs. To optimize the annotation efficiency, the Gene Ontology (GO) categorization was carried out for 186,191 ESTs (BlastN against Uniprot-SwissProt), permitting the categorization of 118,208 ESTs (63.5%). In an attempt to elect a group of the best tags to be validated by RTqPCR, the GO categorization of the tag-related ESTs allowed the in silico identification of 213 upregulated unitags responding basically to abiotic stresses, from which 145 presented no hits after BlastN analysis, probably concerning new genes still uncovered in previous studies. The present report analyzes the sugarcane transcriptome under drought stress, using a combination of high-throughput transcriptome profiling by SuperSAGE with the Solexa sequencing technology, allowing the identification of potential target genes during the stress response

    Relação entre Vivência Acadêmica e Ansiedade em Estudantes Universitários

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    Researches have evaluated the anxiety and college students adaptation to academic life, but few Brazilian studies investigated the relation between these variables. This study aimed to evaluate the student’s degree of academic adaptation and its relation to anxiety and sociodemographic and academic variables. This is a cross-sectional Survey research, part of a multicentric study. Participated 316 students from three institutions: Universidade Federal de São João del-Rei, Universidade Federal de Ouro Preto e Universidade Federal do Triângulo Mineiro. Subjects answered online instruments: Questionário de Vivências Acadêmicas (QVA-r); Escala de Depressão, Ansiedade e Estresse (DASS-21) and sociodemografic/academic questionnaire. Results showed moderate degree of academic adaptation, stronger for the dimensions Carrier, Personal and Interpessonal and moderate levels of anxiety. Multiple regression indicated that higher levels of anxiety were predictor of lower degree of academic adaptation, for Carrier, Study and Institucional. For Carrier, being in a medical course was predictor of lower degree of adaptation. For anxiety, the predictors were being female, being in Nursing course and low age. This research can be considered an advance in this area due to the lack of studies on this issue. Future studies should include others universities and parts of the country.Estudos têm investigado a ansiedade e a adaptação acadêmica em estudantes universitários, mas poucas pesquisas nacionais avaliaram a relação entre estas variáveis. O objetivo deste estudo foi avaliar o grau de adaptação acadêmica dos estudantes e suas relações com ansiedade e variáveis sociodemográficas e acadêmicas. Trata-se de Pesquisa de Levantamento (Survey), de corte transversal, parte de um estudo multicêntrico. Participaram 316 alunos da Universidade Federal de São João del-Rei, Universidade Federal de Ouro Preto e Universidade Federal do Triângulo Mineiro. Foram aplicados os seguintes instrumentos online: Questionário de Vivências Acadêmicas (QVA-r); Escala de Depressão, Ansiedade e Estresse (DASS-21) e Questionário sociodemográfico/acadêmico. Os resultados mostraram grau moderado de adaptação acadêmica, com maiores escores nas dimensões Carreira, Pessoal e Interpessoal e níveis moderados de ansiedade. Na regressão múltipla, maior nível de ansiedade foi preditor de menor adaptação acadêmica, nas dimensões Carreira, Estudo e Institucional. Para a dimensão Carreira, cursar Medicina também foi um fator preditor de menor adaptação. Para ansiedade, os fatores preditores foram: sexo feminino, cursar Enfermagem e ter menor idade. Esta pesquisa pode ser considerada um avanço na área, devido à carência de estudos nesta temática. Estudos futuros deverão incluir outras universidades e regiões do país

    Bat fauna (Mammalia, chiroptera) from guarapuava highlands, Southern Brazil

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    Here, we present an updated list of bats fromGuarapuava highlands, center-southern of Paraná state, southern Brazil. This species list is based on four literature records (secondary data) and data from fieldwork (primary data) in three localities. All species recorded (primary and secondary data) were evaluated by the relative frequency and their conservation status were assessed. The species recorded from fieldwork were also evaluated by relative abundance. A beta diversity analysis was done to verify dissimilarities in the bat fauna among the seven localities. We recorded 28 species in the Guarapuava highlands (14 Vespertilionidae, 10 Phyllostomidae and 4 Molossidae), of which, eight are new records for the region. Sturnira lilium was the most abundant in three localities (primary data), and the most frequent species in all studies (primary and secondary data). Only four species were classified globally as “Near Threatened” or “Data Deficient”, nevertheless they were frequent in this region. The mean regional beta diversity was 0.72, what could be mostly explained by turnover (0.64) rather than by nestedness (0.08). The greatest species richness of the family Vespertilionidae is a common pattern in subtropical and temperate regions as in highlands and mountains. As turnover was the main component of the beta diversity, the communities seemed to be structured mainly by replacement of species among the studied localities. The Guarapuava highlands present a diverse bat fauna, however, this region requires more sampling effort to become well known. © 2019, Universidade Federal do Rio de Janeiro (UFRJ). All rights reserved
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