Vaginal metastasis of a Ewing sarcoma five years after resection of the primary tumor

A 35-year-old female presented with pain and swelling of the distal left radius. A diagnosis of Ewing sarcoma was made and she underwent neoadjuvant chemotherapy and surgery. Macroscopic viable areas remained on the map of the surgical specimen; as such, she was classified as a poor responder and received high dose adjuvant chemotherapy. She remained disease-free for five years, until age 40. A vaginal polyp was then detected during a routine gynaecologic examination. It was removed and histopathology revealed metastatic Ewing sarcoma

A specification patterns system for discrete event systems analysis

As formal verification tools gain popularity, the problem arises of making them more accessible to engineers. A correct understanding of the logics used to express properties of a system's behavior is needed in order to guarantee that properties correctly encode the intent of the verification process. Writing appropriate properties, in a logic suitable for verification, is a skillful process. Errors in this step of the process can create serious problems since a false sense of safety is gained with the analysis. However, when compared to the effort put into developing and applying modeling languages, little attention has been devoted to the process of writing properties that accurately capture verification requirements. In this paper we illustrate how a collection of property patterns can help in simplifying the process of generating logical formulae from informally expressed requirements

Nebraska Agricultural Water Management Demonstration Network (NAWMDN): Integrating Research and Extension/Outreach

Maximizing the net benefits of irrigated plant production through appropriately designed agricultural water management programs is of growing importance in Nebraska, and other western and Midwestern states, because many areas are involved in management and policy changes to conserve irrigation water. In Nebraska, farmers are being challenged to practice conservation methods and use water resources more efficiently while meeting plant water requirements and maintaining high yields. Another challenge Nebraska experiences in it\u27s approximately 3.5‐million‐ha irrigated lands is limited adoption of newer technologies/tools to help farmers better manage irrigation, conserve water and energy, and increase plant water use efficiency. In 2005, the Nebraska Agricultural Water Management Demonstration Network (NAWMDN or Network) was formed from an interdisciplinary team of partners including the Natural Resources Districts (NRD); USDA‐NRCS; farmers from south central, northeast, west central, and western Nebraska; crop consultants; and University of Nebraska‐Lincoln faculty. The main goal of the Network is to enable the transfer of high quality research‐based information to Nebraskans through a series of demonstration projects established in farmers\u27 fields and implement newer tools and technologies to address and enhance plant water use efficiency, water conservation, and reduce energy consumption for irrigation. The demonstration projects are supported by the scientifically‐based field research and evaluation projects conducted at the University of Nebraska‐Lincoln, South Central Agricultural Laboratory located near Clay Center, Nebraska. The Network was formed with only 15 farmers as collaborators in only one of the 23 NRDs in 2005. As of late 2009, the number of active collaborators has increased to over 300 in 12 NRDs and 35 of 93 counties. The Network is impacting both water and energy conservation due to farmers adopting information and newer technologies for irrigation management. The NAWMDN is helping participants to improve irrigation management and efficiency by monitoring plant growth stages and development, soil moisture, and crop evapotranspiration. As a result, they are reducing irrigation water application amounts and associated energy savings is leading to greater profitability to participating farmers. This article describes the goals and objectives of the Network, technical and educational components, operational functions, and procedures used in the NAWMDN. The quantitative impacts in terms of water and energy conservation are reported

NEBRASKA AGRICULTURAL WATER MANAGEMENT DEMONSTRATION NETWORK (NAWMDN): INTEGRATING RESEARCH AND EXTENSION/OUTREACH

Maximizing the net benefits of irrigated plant production through appropriately designed agricultural water management programs is of growing importance in Nebraska, and other western and Midwestern states, because many areas are involved in management and policy changes to conserve irrigation water. In Nebraska, farmers are being challenged to practice conservation methods and use water resources more efficiently while meeting plant water requirements and maintaining high yields. Another challenge Nebraska experiences in it\u27s approximately 3.5‐million‐ha irrigated lands is limited adoption of newer technologies/tools to help farmers better manage irrigation, conserve water and energy, and increase plant water use efficiency. In 2005, the Nebraska Agricultural Water Management Demonstration Network (NAWMDN or Network) was formed from an interdisciplinary team of partners including the Natural Resources Districts (NRD); USDA‐NRCS; farmers from south central, northeast, west central, and western Nebraska; crop consultants; and University of Nebraska‐Lincoln faculty. The main goal of the Network is to enable the transfer of high quality research‐based information to Nebraskans through a series of demonstration projects established in farmers\u27 fields and implement newer tools and technologies to address and enhance plant water use efficiency, water conservation, and reduce energy consumption for irrigation. The demonstration projects are supported by the scientifically‐based field research and evaluation projects conducted at the University of Nebraska‐Lincoln, South Central Agricultural Laboratory located near Clay Center, Nebraska. The Network was formed with only 15 farmers as collaborators in only one of the 23 NRDs in 2005. As of late 2009, the number of active collaborators has increased to over 300 in 12 NRDs and 35 of 93 counties. The Network is impacting both water and energy conservation due to farmers adopting information and newer technologies for irrigation management. The NAWMDN is helping participants to improve irrigation management and efficiency by monitoring plant growth stages and development, soil moisture, and crop evapotranspiration. As a result, they are reducing irrigation water application amounts and associated energy savings is leading to greater profitability to participating farmers. This article describes the goals and objectives of the Network, technical and educational components, operational functions, and procedures used in the NAWMDN. The quantitative impacts in terms of water and energy conservation are reported

Cyclic shear tests on RC precast beam-to-column connections retrofitted with a three-hinged steel device

Recent European earthquakes demonstrated that the seismic response of RC precast structures can be significantly influenced by the connection systems. Moreover, during past seismic events, many failures of the beam-to-column connections occurred due to their inadequate strength under seismic loads. The seismic safety of these connections has a crucial role in the overall seismic capacity of existing precast structures. A new connection system is employed as a retrofitting solution for a damaged beam-to-column connection and its cyclic shear performance is investigated by means of two cyclic shear tests on two different configurations. In both the experimental tests, the results demonstrate an efficient behavior of the retrofitted connections under horizontal cyclic loads. The comparison between the performance of the investigated connection and the response of a typical beam-to-column dowel connection allows to discuss the main critical features of the dowel connection system

Importance of allogeneic T-cells for disease control after stem cell transplantation for high-risk Langerhans cell histiocytosis

Reduced intensity conditioning followed by allogeneic SCT (RIC-SCT) has recently emerged as promising new salvage option for children suffering from Langerhans cell histiocytosis (LCH) with risk organ involvement and failure to conventional therapy. We report on the posttransplant course of female toddler with high-risk LCH, who achieved complete remission after RIC-SCT, despite a posttransplant chimerism constellation, in which only the T-cell subset proved to be of donor origin in the long-term. We therefore suggest that allogeneic T-cells have played a crucial role in controlling disease activity in this patient and may exert the major curative effect after RIC-SCT for LCH

Carboxypeptidase G2 rescue in patients with methotrexate intoxication and renal failure

The methotrexate (MTX) rescue agent carboxypeptidase G2 (CPDG2) rapidly hydrolyses MTX to the inactive metabolite DAMPA (4-[[2,4-diamino-6-(pteridinyl)methyl]-methylamino]-benzoic acid) and glutamate in patients with MTX-induced renal failure and delayed MTX excretion. DAMPA is thought to be an inactive metabolite of MTX because it is not an effective inhibitor of the MTX target enzyme dihydrofolate reductase. DAMPA is eliminated more rapidly than MTX in these patients, which suggests a nonrenal route of elimination. In a phase II study (May 1997–March 2002), CPDG2 was administered intravenously to 82 patients at a median dose of 50 U kg−1 (range 33–60 U kg−1). Eligible patients for this study had serum MTX concentrations of >10 μM at 36 h or >5 μM at 42 h after start of MTX infusion and documented renal failure (serum creatinine ⩾1.5 times the upper limit of normal). Immediately before CPDG2 administration, a median MTX serum level of 11.93 μM (range 0.52–901 μM) was documented. Carboxypeptidase G2 was given at a median of 52 h (range 25–178 h) following the start of an MTX infusion of 1–12 g m−2 4–36 h−1 and resulted in a rapid 97% (range 73–99%) reduction of the MTX serum level. Toxicity related to CPDG2 was not observed. Toxicity related to MTX was documented in about half the patients; four patients died despite CPDG2 administration due to severe myelosuppression and septic complications. In conclusion, administration of CPDG2 is a well-tolerated, safe and a very effective way of MTX elimination in delayed excretion due to renal failure

Consensus criteria for sensitive detection of minimal neuroblastoma cells in bone marrow, blood and stem cell preparations by immunocytology and QRT-PCR: recommendations by the International Neuroblastoma Risk Group Task Force

Disseminating disease is a predictive and prognostic indicator of poor outcome in children with neuroblastoma. Its accurate and sensitive assessment can facilitate optimal treatment decisions. The International Neuroblastoma Risk Group (INRG) Task Force has defined standardised methods for the determination of minimal disease (MD) by immunocytology (IC) and quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR) using disialoganglioside GD2 and tyrosine hydroxylase mRNA respectively. The INRG standard operating procedures (SOPs) define methods for collecting, processing and evaluating bone marrow (BM), peripheral blood (PB) and peripheral blood stem cell harvest by IC and QRT-PCR. Sampling PB and BM is recommended at diagnosis, before and after myeloablative therapy and at the end of treatment. Peripheral blood stem cell products should be analysed at the time of harvest. Performing MD detection according to INRG SOPs will enable laboratories throughout the world to compare their results and thus facilitate quality-controlled multi-centre prospective trials to assess the clinical significance of MD and minimal residual disease in heterogeneous patient groups