Strukturni pokazatelji grozda i bobice klonova sorte Burgundac crni i njihov uticaj na hemijske karakteristike vina

Research were carried out in the vineyard of Experimental field “Radmilovac” at Faculty of Agriculture in Belgrade and included 3 Pinot Noir clones: R4, 115 and 667. For the purpose of research the structural indicators of grape and berries by are selected ten vines of each clone. Wine of the clones was prepared by procedure microvinification. Of all the tested clones, clone R4 had the best relationship structure of the bunch (bunch stem - 3.91%, and berries - 96.09%) and the highest concentration of total phenolic compounds. A strong correlation between the skin in the berries and the total phenolic compounds in all wine was founded.Istraživanja su obavljena na OD “Radmilovac” Poljoprivrednog fakulteta Univerziteta u Beogradu i obuhvatila su tri klona sorte Burgundac crni: R4, 115 i 667. Za ispitivanja strukturnih pokazatelja grozda i bobice odabrano je po 10 čokota svakog klona. Vino od klonova je spravljeno postupkom mikrovinifikacije. Od svih ispitivanih klonova, klon R4 je imao najpovoljniji odnos strukture grozda (ogrozdina - 3,91%; bobice - 96,09%), kao i najveću koncentraciju ukupnih fenolnih jedinjenja u vinu. Kod ispitivanih klonova je utvrđena jaka korelaciona zavisnost između udela pokožice u bobici i količine ukupnih fenolnih jedinjenja u vinu

Modulatory effect of curcumin on ketamine-induced toxicity in rat thymocytes: Involvement of reactive oxygen species (ROS) and the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway

Ketamine is a widely used anesthetic in pediatric clinical practice. Previous studies have demonstrated that ketamine induces neurotoxicity and has a modulatory effect on the cells of the immune system. Here, we evaluated the potential protective effect and underlying mechanisms of natural phenolic compound curcumin against ketamine-induced toxicity in rat thymocytes. Rat thymocytes were exposed to 100 µM ketamine alone or combined with increasing concentrations of curcumin (0.3, 1, and 3 μM) for 24 hours. Cell viability was analyzed with CCK-8 assay kit. Apoptosis was analyzed using flow cytometry and propidium iodide as well as Z-VAD-FMK and Z-LEHD-FMK inhibitors. Reactive oxygen species (ROS) production and mitochondrial membrane potential [MMP] were measured by flow cytometry. Colorimetric assay with DEVD-pNA substrate was used for assessing caspase-3 activity. Involvement of phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway was tested with Wortmannin inhibitor. Ketamine induced toxicity in cells, increased the number of hypodiploid cells, caspase-3 activity and ROS production, and inhibited the MMP. Co-incubation of higher concentrations of curcumin (1 and 3 μM) with ketamine markedly decreased cytotoxicity, apoptosis rate, caspase-3 activity, and ROS production in rat thymocytes, and increased the MMP. Application of Z-VAD-FMK (a pan caspase inhibitor) or Z-LEHD-FMK (caspase-9 inhibitor) with ketamine effectively attenuated the ketamine-induced apoptosis in rat thymocytes. Administration of Wortmannin (a PI3K inhibitor) with curcumin and ketamine significantly decreased the protective effect of curcumin on rat thymocytes. Our results indicate that ketamine-induced toxicity in rat thymocytes mainly occurs through the mitochondria-mediated apoptotic pathway and that the PI3K/Akt signaling pathway is involved in the anti-apoptotic effect of curcumin

Ecologically friendly chitosan-montmorillonite bio-nanocomposite as adsorbent for textile dyes from aqueous solutions

The bio-nanocomposite of montmorillonite and natural biopolymer chitosan (C-MM) was synthesized. Intercalation of chitosan in form of monolayer into the interlamellar space of montmorillonite was confirmed by XRD and IR analysis. The adsorption of textile dyes: Acid Yellow 99 (AY99), Acid Orange 10 (AO10) and Reactive Black 5 (RB5) onto C-MM was investigated. The adsorption was performed with the respect to adsorption time, pH and initial dye concentration. The kinetics of adsorption obeyed pseudo-second-order of kinetics and was the most efficient in acidic pH. Langmuir model best described the adsorption of AY99 and AO10, while RB5 adsorption isotherm was best fitted with Freundlich model

A Deficiência de vitamina d está associada com níveis aumentados de il-17 e tnfα em pacientes com insuficiência cardíaca crônica

FUNDAMENTO: Estudos recentes revelaram uma forte associação entre o estado de vitamina D (VD) e a insuficiência cardíaca crônica (ICC). Hoje, é normalmente aceito que a resposta imune pró-inflamatória é subjacente ao desenvolvimento de ICC. OBJETIVO: Uma vez que a VD possui propriedades anti-inflamatórias, pesquisamos o seu impacto sobre as citocinas envolvidas na ICC, como TNFα e IL-17, em pacientes portadores de ICC. MÉTODOS: Foi extraído sangue de quarenta pacientes com ICC secundária à hipertensão arterial e/ou doença coronariana. Os níveis de VD status, IL-17 e TNFαforam avaliados através de 25-hidroxi VD3 EIA e ELISA de citocinas. Também foram realizadas avaliação clínica e ecocardiograma. RESULTADOS: Pacientes idosos com ICC em Nis (Sudeste da Europa, latitude 43ºN) apresentaram níveis de 25-hidroxi VD3 abaixo do normal. Nossos dados demonstraram que pacientes com ICC secundária à hipertensão arterial têm níveis significativamente menores de 25-hidroxi VD3, e maiores de TNFαe IL-17A, se comparados com os níveis de pacientes com ICC secundária à doença coronariana. CONCLUSÃO: É demonstrado aqui que, mesmo em regiões com muitos dias ensolarados a deficiência de VD é motivo de preocupação. Os dados sugerem que o déficit de VD contribui para os elevados níveis de IL-17 e TNFα e, assim, contribuir ao desenvolvimento de ICC

Towards equal access to health services in Serbia

Serbia has a comprehensive universal health system with free access to health care, but there are inequities in the utilisation of health services. Some vulnerable groups, such as those living in poverty or Roma people in settlements, have more barriers in accessing health care. Financial constraints are the main reason for unmet needs, in particular for the less educated and the poorest. Although citizens are generally satisfied with public and private health care services, a significant number of patients are on waiting lists. Therefore, reaching equal access to health services should be one of the leading health policy goals

Towards equal access to health services in Serbia

Serbia has a comprehensive universal health system with free access to health care, but there are inequities in the utilisation of health services. Some vulnerable groups, such as those living in poverty or Roma people in settlements, have more barriers in accessing health care. Financial constraints are the main reason for unmet needs, in particular for the less educated and the poorest. Although citizens are generally satisfied with public and private health care services, a significant number of patients are on waiting lists. Therefore, reaching equal access to health services should be one of the leading health policy goals