47 research outputs found
Optimization issues in phenotyping plateforms
International audienceThe combined effects of climate change and population growth have made agriculture appear as one of the most important challenge of the century. In order to be able to produce food for everyone in the coming decades, there is an urgent need to build more knowledge about plants, and how to grow them in a sustainable way, i.e., how to produce enough biomass without resorting to pollutant chemicals inputs. Hence, plant biologists need to understand what makes plants resistant to diseases, parasites, insects and hydric stress, among other environment effects. The functional plant body, called the phenotype, is the result of the combination of the plan genotype with the environment: two plants with the same DNA will not have the same development under different environment conditions. As the so-called \textit{plant performance} is the result of phenotype, a lot of effort has recently been devoted to study plants phenotype. More specifically, biologists run large scale experiments on hundreds or thousands of plants, for example to determine how resistant is a particular plant to a wide range of environment conditions. To this end, the plants are settled in pots, and these pots are moved by a conveyor belt from a cultivation chamber to different quantitative automated measurement stations, where RGB cameras, spectrography and other techniques are employed to monitor plants growth on a regular basis. The European project EPPN2020 aims at gathering a large number of such facilities to share experience and accelerate the research progresses in plant biology. It currently involves 31 plant phenotyping platforms and 21 partners across Europe from 12 countries (including the Slovak University of Agriculture in Nitra), under the coordination of INRA, with a budget of \euro 10 million for four years. It can be observed that the phenotyping platforms available in EPPN2020 and everywhere else in the world have a very similar structure
Alfalfa crops amended with MSW compost can compensate the effect of salty water irrigation depending on the soil texture.
The availability of water resources of marginal quality such as drainage water or high-saltcontaining groundwater is turning into an important issue in Tunisia and other countrieswith scarce water resources. A pot experiment was carried out to evaluate plant produc-tion, nutrient content and heavy metal bioaccumulation in agricultural soils amended withMSW compost and irrigated with salty water, by using two different soil textures (clay andsandy). Salt water supply decreased plant dry yield in both soils. Salt stress had signifi-cantly reduction in plant biomass in sandy soil compared to those in clay soil (biomass ofdry weight is significantly higher in clay soil than those in sandy soil in presence of salt:percentage of growth compared to control was 55% for clay soil and 45% for sandy soil).The application of Municipal Solid Wastes (MSW) Compost increased significantly alfalfaproductivity in both soils (Dry weight is significantly higher in presence of compost: 140%for clay soil and 125% for sandy soil). In non-amended soil, the growth was reduced signifi-cantly by salt stress (50% in clay soil, 26% in sandy compared to the soils without salty waterapplication). Plants irrigated with salty water accumulated much more sodium on sandysoil (1.74 mmol g−1) than on clay one (0.87 mmol g−1DW). Compost did not reduce sodiumaccumulation in aerial parts on sandy soil, whereas it slightly reduced it in those grownon clay soil. Zinc (Zn), Copper (Cu), Lead (Pb) and Cadmium (Cd) concentrations showedthe same trend for both soil types. They increased statistically significant by salinity to124-189%, the highest rise was found in Cu concentration on clay soil. The order of metaluptake was: Zn > Cu > Pb > Cd. A higher significant shoot accumulation of heavy metals (upto 305% of the control) was noticed in the presence of compost with no difference betweensalt-treated and non-treated. MSW compost amendment caused an increase of the studiedheavy metals in alfalfa shoots grown that was higher on sandy soils than clay soils. Heavymetals in plants remained lower than phytotoxic level and these level of accumulation did not restrain the enhancement of alfalfa yield. MSW compost at 40 t ha−1was convinent todo not attend phytotoxic level. These results suggest that MSW compost compensates, atleast partially, the negative effect of salinity on plant growth and nutrient uptake and thatit is important to know soil texture to apply compost to remediate salty degraded soils.© 2017 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved
The impact of PEG-induced drought stress on seed germination and seedling growth of different bread wheat genotypes
Wheat is an important crop, used as staple food in numerous countries around the world. However, wheat productivity is low in the developing world due to several biotic and abiotic stresses, particularly drought stress. Non-availability of drought-tolerant wheat genotypes at different growth stages is the major constraint in improving wheat productivity in the developing world. Therefore, screening/developing drought-tolerant genotypes at different growth stages could improve the productivity of wheat. This study assessed seed germination and seedling growth of eight wheat genotypes under polyethylene glycol (PEG)-induced stress. Two PEG-induced osmotic potentials (i.e., -0.6 and -1.2 MPa) were included in the study along with control (0 MPa). Wheat genotypes included in the study were ‘KLR-16’, ‘B6’, ‘J10’, ‘716’, ‘A12’, ‘Seher’, ‘KTDH-16’, and ‘J4’. Data relating to seed germination percentage, root and shoot length, fresh and dry weight of roots and shoot, root/shoot length ratio and chlorophyll content were recorded. The studied parameters were significantly altered by individual and interactive effects of genotypes and PEG-induced osmotic potentials. Seed germination and growth parameters were reduced by osmotic potentials; however, huge differences were noted among genotypes. A reduction of 32.83 to 53.50% was recorded in seed germination, 24.611 to 47.75% in root length, 37.83 to 53.72% in shoot length, and 53.35 to 65.16% in root fresh weight. The genotypes, ‘J4’, ‘KLR-16’ and ‘KTDH-16’, particularly ‘J4’ better tolerated increasing osmotic potentials compared to the rest of the genotypes included in the study. Principal component analysis segregated these genotypes from the rest of the genotypes included in the study indicated that these can be used in the future studies to improve the drought tolerance of wheat crop. The genotype ‘J4’ can be used as a breeding material to develop drought resistant wheat genotypes
Quantitative response of wheat to sowing dates and irrigation regimes using ceres-wheat model
An experiment was conducted at Punjab Agricultural University, Ludhiana during 2014–15 and 2015–16, keeping four sowing dates {25th Oct (D1), 10th Nov (D2), 25th Nov (D3) and 10th Dec (D4)} in main plots and five irrigation schedules {irrigation at 15 (FC15), 25 (FC25), 35 (FC35) and 45 (FC45) % depletion of soil moisture from field capacity (FC) and a conventional practice} in sub plots. The objective of the study was to evaluate the performance of CERES-Wheat model for simulating yield and water use under varying planting and soil moisture regimes. The simulated and observed grain yield was higher in D1, with irrigation applied at FC15 as compared to all other sowing date and irrigation regime combinations. Simulated grain yield decreased by 19% with delay in sowing from 25th October to 10th December because of 8% reduction in simulated crop evapotranspiration. Simulated evapotranspiration decreased by 16%, wheat grain yield by 23% and water productivity by 15% in drip irrigation at 45% depletion from field capacity as compared to drip irrigation at 15% of field capacity. It was further revealed that the model performed well in simulating the phenology, water use and yield of wheat
Photosynthetic responses of sun- and shade-grown barley leaves to high light: is the lower PSII connectivity in shade leaves associated with protection against excess of light?
Frequently asked questions about chlorophyll fluorescence, the sequel
[EN] Using chlorophyll (Chl) a fluorescence many aspects of the photosynthetic apparatus can be studied, both in vitro and, noninvasively, in vivo. Complementary techniques can help to interpret changes in the Chl a fluorescence kinetics. Kalaji et al. (Photosynth Res 122: 121-158, 2014a) addressed several questions about instruments, methods and applications based on Chl a fluorescence. Here, additionalChl a fluorescence-related topics are discussed again in a question and answer format. Examples are the effect of connectivity on photochemical quenching, the correction of F-V/F-M values for PSI fluorescence, the energy partitioning concept, the interpretation of the complementary area, probing the donor side of PSII, the assignment of bands of 77 K fluorescence emission spectra to fluorescence emitters, the relationship between prompt and delayed fluorescence, potential problems when sampling tree canopies, the use of fluorescence parameters in QTL studies, the use of Chl a fluorescence in biosensor applications and the application of neural network approaches for the analysis of fluorescence measurements. The answers draw on knowledge fromdifferent Chl a fluorescence analysis domains, yielding in several cases new insights.Kalaji, H.; Schansker, G.; Brestic, M.; Bussotti, F.; Calatayud, A.; Ferroni, L.; Goltsev, V.... (2017). Frequently asked questions about chlorophyll fluorescence, the sequel. 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Evaluation of Hyperspectral Reflectance Parameters to Assess the Leaf Water Content in Soybean
Nondestructive assessment of water content and water stress in plants is an important component in the rational use of crop irrigation management in precision agriculture. Spectral measurements of light reflectance in the UV/VIS/NIR region (350–1075 nm) from individual leaves were acquired under a rapid dehydration protocol for validation of the remote sensing water content assessment in soybean plants. Four gravimetrical approaches of leaf water content assessment were used: relative water content (RWC), foliar water content as percent of total fresh mass (FWCt), foliar water content as percent of dry mass (FWCd), and equivalent water thickness (EWT). Leaf desiccation resulted in changes in optical properties with increasing relative reflectance at wavelengths between 580 and 700 nm. The highest positive correlations were observed for the relations between the photochemical reflectance index (PRI) and EWT (rP = 0.860). Data analysis revealed that the specific water absorption band at 970 nm showed relatively weaker sensitivity to water content parameters. The prediction of leaf water content parameters from PRI measurements was better with RMSEs of 12.4% (rP = 0.786), 9.1% (rP = 0.736), and 0.002 (rP = 0.860) for RWC, FWCt, and EWT (p < 0.001), respectively. The results may contribute to more efficient crop water management and confirmed that EWT has a statistically closer relationship with reflectance indices than other monitored water parameters
Genotypically Identifying Wheat Mesophyll Conductance Regulation under Progressive Drought Stress
AbstractPhotosynthesis limitation by CO2 flow constraints from sub-stomatal cavities to carboxylation sites in chloroplasts under drought stress conditions is, at least in some plant species or crops not fully understood, yet. Leaf mesophyll conductance for CO2 (gm) may considerably affect both photosynthesis and water use efficiency in plants under drought conditions. The aim of our study was to detect the responses of gm in leaves of four winter wheat (Triticum aestivum L.) genotypes from different origins under long-term progressive drought. Based on the measurement of gas-exchange parameters the variability of genotypic responses was analyzed at stomatal (stomata closure) and non-stomatal (diffusional and biochemical) limits of net CO2 assimilation rate (AN). In general, progressive drought caused an increasing leaf diffusion resistance against CO2 flow leading to the decrease of AN, gm and stomatal conductance (gs), respectively. Reduction of gm also led to inhibition of carboxylation efficiency (Vcmax). On the basis of achieved results a strong positive relationship between gm and gs was found out indicating a co-regulation and mutual independence of the relationship under the drought conditions. In severely stressed plants, the stomatal limitation of the CO2 assimilation rate was progressively increased, but to a less extent in comparison to gm, while a non-stomatal limitation became more dominant due to the prolonged drought. Mesophyll conductance (gm) seems to be a suitable mechanism and parameter for selection of improved diffusional properties and photosynthetic carbon assimilation in C3 plants, thus explaining their better photosynthetic performance at a whole plant level during periods of drought
Applying hyperspectral imaging to explore natural plant diversity towards improving salt stress tolerance
Salinity represents an abiotic stress constraint affecting growth and productivity of plants in many regions of the world. One of the possible solutions is to improve the level of salt resistance using natural genetic variability within crop species. In the context of recent knowledge on salt stress effects and mechanisms of salt tolerance, this review present useful phenomic approach employing different non-invasive imaging systems for detection of quantitative and qualitative changes caused by salt stress at the plant and canopy level. The focus is put on hyperspectral imaging technique, which provides unique opportunities for fast and reliable estimate of numerous characteristics associated both with various structural, biochemical and physiological traits. The method also provides possibilities to combine plant and canopy analyses with a direct determination of salinity in soil. The future perspectives in salt stress applications as well as some limits of the method are also identified. (C) 2016 Elsevier B.V. All rights reserved.</p
Comparative analysis of bioactive phenolic compounds composition from 26 medicinal plants
Bioactive phenolic compounds are powerful antioxidants in traditionally used medicinal and industrial crop plants and have attracted increased interest in the last years in their application and role in non-destructive methodology for pre-screening analysis of some stress factors. In this study the qualitative target was linked with future possible applications of received data for improving non-destructive methodology as well as for improving existing knowledge regarding antioxidant content in some plant species. Comparative analysis of total phenolics, flavonoid contents, phenolic acid composition, and antioxidant activity in known east central Europe medicinal and industrial crop plants of 26 species of families Asteraceae, Rosaceae and Lamiaceae was done. Among the investigated leaf extracts the highest total phenolic, total flavonoid contents and antioxidant activity have been seen for Stachys byzantine L. (Lamiaceae), Calendula officinalis L. (Asteraceae) and for Potentilla recta L. (Rosaceae). The highest syringic acid content has been found in the leaf extracts of plant family Asteraceae – in the range from 0.782 to 5.078 mg g−1 DW. The representative’s family Rosaceae has a higher content of p-anisic acid in the range 0.334–3.442 mg g−1DW compared to the leaf extracts of families Lamiaceae and Asteraceae. The comparative study showed significant differences of content of phenolic acids in the leaf extracts of different representative’s families Rosaceae, Asteraceae and Lamiaceae. We suggest that the presence of some phenolic acids can be used as a possible marker for family botanical specifications of representative families Asteraceae and Rosaceae. It was supposed that some pharmacological effects can be connected with the analyzed data. Keywords: Phenolic compounds, Flavonoids, Phenolic acids, Antioxidant activity, Asteraceae, Rosaceae, Lamiacea