Molecular signatures of cell migration in C. elegans Q neuroblasts.

Metazoan cell movement has been studied extensively in vitro, but cell migration in living animals is much less well understood. In this report, we have studied the Caenorhabditis elegans Q neuroblast lineage during larval development, developing live animal imaging methods for following neuroblast migration with single cell resolution. We find that each of the Q descendants migrates at different speeds and for distinct distances. By quantitative green fluorescent protein imaging, we find that Q descendants that migrate faster and longer than their sisters up-regulate protein levels of MIG-2, a Rho family guanosine triphosphatase, and/or down-regulate INA-1, an integrin alpha subunit, during migration. We also show that Q neuroblasts bearing mutations in either MIG-2 or INA-1 migrate at reduced speeds. The migration defect of the mig-2 mutants, but not ina-1, appears to result from a lack of persistent polarization in the direction of cell migration. Thus, MIG-2 and INA-1 function distinctly to control Q neuroblast migration in living C. elegans

Mass customization of teaching and learning in organizations

In search of methods that improve the efficiency of teaching and training in organizations, several authors point out that mass customization (MC) is a principle that covers individual needs of knowledge and skills and, at the same time, limits the development costs of customized training to those of mass training. MC is proven and established in the economic sector, and shows high potential for continuing education, too. The paper explores this potential and proposes a multidisciplinary, pragmatic approach to teaching and training in organizations. The first section of the paper formulates four design principles of MC deduced from an examination of economics literature. The second section presents amit™, a frame for mass customized training, designed according to the principles presented in the first section. The evaluation results encourage the further development and use of mass customized training in continuing education, and offer suggestions for future research

Supply-chain modelling and control under proportional inventory-replenishment policies

A novel state-space model of a multi-node supply chain is presented, controlled via local proportional inventory-replenishment policies. The model is driven by a stochastic sequence representing customer demand. The model is analysed under stationarity conditions and a simple recursive scheme is developed for updating its covariance matrix. This allows us to characterise the ‘bullwhip effect’ (demand amplification) in the chain and to solve an optimisation problem for a three-node model involving the minimisation of inventory subject to a probabilistic constraint on downstream demand. Finally, issues related to estimation schemes based on local historical data are briefly discussed

Approximation Algorithms for Stochastic Inventory Control Models

Approximation Algorithms for Stochastic Inventory Control Model

The C. elegans tailless/Tlx homolog nhr-67 regulates a stage-specific program of linker cell migration in male gonadogenesis

Cell migration is a common event during organogenesis, yet little is known about how migration is temporally coordinated with organ development. We are investigating stage-specific programs of cell migration using the linker cell (LC), a migratory cell crucial for male gonadogenesis of C. elegans. During the L3 and L4 larval stages of wild-type males, the LC undergoes changes in its position along the migratory route, in transcriptional regulation of the unc-5 netrin receptor and zmp-1 zinc matrix metalloprotease, and in cell morphology. We have identified the tailless homolog nhr-67 as a cell-autonomous, stage-specific regulator of timing in LC migration programs. In nhr-67-deficient animals, each of the L3 and L4 stage changes is either severely delayed or never occurs, yet LC development before the early L3 stage or after the mid-L4 stage occurs with normal timing. We propose that there is a basal migration program utilized throughout LC migration that is modified by stage-specific regulators such as nhr-67

Hierarchical models for the anlaysis of species distributions and abundances: development and applications

There is a strong need for statistical methods that can maximize the utility of ecological data while providing accurate estimates of species abundances and distributions. This dissertation aims to build on current statistical models using Bayesian hierarchical approaches to advance these methods. Chapters one, two, and three utilize a multi-species modeling framework to estimate species occurrence probabilities. Chapter one presents a model to assess the community response of breeding birds to habitat fragmentation. The results demonstrate the importance of understanding the responses of both individual, and groups of species, to environmental heterogeneity while illustrating the utility of hierarchical models for inference about species richness. Chapter two demonstrates how the multi-species modeling framework can be used to evaluate conservation actions through a component that incorporates species-specific responses to management treatments. In Chapter three, I develop a method for validating predictions generated by the multi-species model that accounts for detection biases in evaluation data. I build competing models using wetland breeding amphibian data and test their abilities to predict occupancy at unsampled locations. Chapters four and five develop count models that are used to estimate population abundances in relation to environmental and climate variables. In Chapter four, I employ a Poisson regression designed to determine how climate affects the annual abundances of migrating monarch butterflies. I incorporate the climate conditions experienced both during a spring migration phase, as well as during summer recruitment. In Chapter five, I analyze sea duck data to characterize the spatial and temporal distributions along the U.S. and Canadian Atlantic coast. I model count data for five species using a zero-inflated negative binomial model that includes latitude, habitat covariates, and the North Atlantic Oscillation. The results from these two chapters demonstrate how Bayesian models can be used to elucidate complicated species-climate relationships. The chapters of this dissertation illustrate creative development and application of advanced statistical methods to complex biological systems. These applications provide a practical framework for dealing with highly aggregated species and uneven species distributions in community analyses, as well as a method for evaluating occurrence estimates that accounts for detection biases. My results highlight the dynamic relationships between population and community structure, habitat, and climate

Keepers of the vision : students\u27 perceptions of themselves as creators and changers.

This qualitative study undertook an exploration into the connections between creativity and change/transformation. Its purpose was to begin to understand how creativity could be used as a tool for empowerment, liberation, and transformation, and how creative techniques could be incorporated into educational efforts. Participants were students who were enrolled in an undergraduate course designed and taught by the researcher, entitled Creativity and Change. Using their coursework and interviews, this research explored how these student/participants perceived of themselves as creative individuals and as persons capable of making change in their lives and in the world, how they connected the two concepts of creativity and change, and how they utilized creative processes in envisioning change strategies. This study broke ground in two areas of creativity research: (1) looking at the effects and benefits of a creativity class from the perspective of the students who were enrolled in it, and (2) expanding on the research on climate for creativity by connecting creativity to personal and social change efforts, again, from the perspective of students. The results of this study are a beginning to better understanding the multiple aspects of creativity--expression, process, products--and their importance ta self-development, empowerment, and liberation in education. The responses of the students who participated in this study were encouraging in supporting the premise that there are multiple connections between creativity and change. They demonstrated the effects of creativity in increasing self-knowledge and self-esteem, making connections between people, envisioning solutions to social problems, expanding perceptions to incorporate different perspectives and viewpoints, breaking boundaries, and taking the risks that transformation demands. These student/participants demonstrated, through the discovery of their own creative, capable selves, a sense of empowerment arising from the knowledge that I CAN. Being able to think outside of conventional structures and paradigms, challenging dualistic thought, and incorporating holistic teaching and learning strategies are some of the strengths and gifts of creativity. This study has shown that the openness and imagination of creative thought and behavior can become a tool to dismantle the injustices of the world and create new, transformative paradigms

Brand Identity and Online Self-Customisation Usefulness Perception

Online self-customisation (OSC) enables customers to tailor their preferences to certain product features via a brand-hosted online platform. Recent literature has given increasing attention to how consumers value OSC. However, extant research is characterised by a scarcity of understanding the effects of brand identity and individual differences on consumer responses to OSC. The purpose of this paper is to examine the mediating role of trust and the moderating role of need for uniqueness on the effects of brand identity prestige and brand identity similarity on consumer perceived usefulness of OSC. A field survey, through mall intercept, was conducted to test this conceptual framework. Our findings advance this field by finding that, not only the brand identity and consumer need for uniqueness, but also the interaction between them may affect consumers’ evaluation of OSC