Expression levels of the tetratricopeptide repeat protein gene ttc39b covary with carotenoid-based skin colour in cichlid fish

Carotenoid pigments play a major role in animal body colouration, generating strong interest in the genes involved in the metabolic processes that lead from their dietary uptake to their storage in the integument. Here, we used RNA sequencing (RNA-Seq) to test for differentially expressed genes in a taxonomically replicated design using three pairs of related cichlid fish taxa from the genera Tropheus and Aulonocara. Within each pair, taxa differed in terms of red and yellow body colouration, and high‐performance liquid chromatography (HPLC) analyses of skin extracts revealed different carotenoid profiles and concentrations across the studied taxa. Five genes were differentially expressed in all three yellow–red skin contrasts (dhrsx, nlrc3, tcaf2, urah and ttc39b), but only the tetratricopeptide repeat protein-coding gene ttc39b, whose gene product is linked to mammalian lipid metabolism, was consistently expressed more highly in the red skin samples. The RNA-Seq results were confirmed by quantitative PCR. We propose ttc39b as a compelling candidate gene for variation in animal carotenoid colouration. Since differential expression of ttc39b was correlated with the presence/absence of yellow carotenoids in a previous study, we suggest that ttc39b is more likely associated with the concentration of total carotenoids than with the metabolic formation of red carotenoids

Is biparental defence driven by territory protection, offspring protection or both?

In many animal systems, the defence of a territory or nest coincides with the defence of offspring, and it is often unclear whether the defence behaviour exists for the purpose of offspring protection, territory protection or a combination of both. In species with biparental care, the drivers of defence behaviours in males may differ from those in females, particularly if there are sex-specific fitness benefits from the current brood or territory. In this study, we present field experiments aimed at elucidating sex differences in the drivers behind nest defence in a fish species (Variabilichromis moorii, Cichlidae) with biparental care. High rates of cuckoldry in this species create asymmetries in brood value between mates, suggesting that increased brood survival may be a weaker driver of male than of female defence effort. We conducted parent and offspring removal experiments and found that following the removal of their mates, single males lost significantly more fry than single females. Some single males lost their territories, while others constricted their space use, suggesting that experimental divorce decreased their success in territory defence. Removal of fry from territories guarded by both parents caused the defence behaviour to become more male biased, that is, males contributed relatively more to defence without any fry present. Our results suggest that, relative to that of females, male defence behaviour is more strongly driven by territory retention than by brood protection. Nevertheless, the presence of males improves parents’ abilities to keep territory intruders at bay, and thus probably confers benefits to broods. We conclude that the drivers of defence behaviours differ between the sexes in this species, with defence behaviours aligning well with the traditional definition of parental care for females, but less so for males.publishe