ANALYSIS OF LATERAL DISPLACEMENT AND EVALUATION OF TREATMENT MEASURES OF CURVED BEAM: A CASE STUDY

The curved beam bridge exhibits lateral displacement during construction and operation. Taking a curved beam bridge as an example, the status of lateral displacement of the bridge is investigated in detail in this paper. To understand the mechanism of the curved beam lateral displacement, further to determine the curved beam lateral displacement under temperature effect, using ANSYS software to establish solid element model of the curved beam, steady state thermal analysis method is applied to analyze temperature field. Based on the analysis, the lateral displacement under temperature effect is analyzed. Then in order to further explain the lateral displacement mechanism, to discuss the frictional force causing the residual deformation of the rubber bearing to make the lateral displacement of the curved beam, the mechanical mechanism of curved beam under temperature effect is approximately analyzed. On the basis of clarifying the mechanism of lateral displacement, the paper puts forward the reinforcement measures for the curved beam bridge. In order to verify the treatment effect, long-term displacement monitoring is performed on the bridge. Numerical studies and monitoring data show that temperature is the main factor that causes the lateral displacement. Monitoring data over the past year shows that the displacement of the bearing is less than the value of allowable displacement after the reinforcement measures are adopted, and the bridge is in a safe state

RIdeogram : Drawing SVG graphics to visualize and map genome-wide data on the idiograms

Background. Owing to the rapid advances in DNA sequencing technologies, whole genome from more and more species are becoming available at increasing pace. For whole-genome analysis, idiograms provide a very popular, intuitive and effective way to map and visualize the genome-wide information, such asGCcontent, gene and repeat density, DNA methylation distribution, genomic synteny, etc. However, most available software programs and web servers are available only for a few model species, such as human, mouse and fly, or have limited application scenarios. As more and more non-model species are sequenced with chromosome-level assembly being available, tools that can generate idiograms for a broad range of species and be capable of visualizing more data types are needed to help better understanding fundamental genome characteristics. Results. The R package RIdeogram allows users to build high-quality idiograms of any species of interest. It can map continuous and discrete genome-wide data on the idiograms and visualize them in a heat map and track labels, respectively. Conclusion. The visualization of genome-wide data mapping and comparison allow users to quickly establish a clear impression of the chromosomal distribution pattern, thus making RIdeogram a useful tool for any researchers working with omics.</p

Formamide deionized accelerates the somatic embryogenesis of Cunninghamia lanceolata

Aim of the study: To improve the efficiency of the somatic embryogenesis (SE) in Cunninghamia lanceolata. Area of the study: The study was conducted at Nanjing Forestry University (Nanjing, China). Material and methods: Immature cones of C. lanceolata, genotype 01A1 which was planted in Yangkou State-owned Forest Farm (Fujian, China), were used to induced callus. These calli were used to induce SE, concentration gradients of 0 g/L, 0.01134 g/L, 0.1134 g/L, 1.1134 g/L and 11.34 g/L of FD was added, to explore the optimal concentration for promoting SE of C. lanceolata. Main results: Low concentration of FD promoted the maturation of somatic embryos, while high concentration of FD lead to browning of embryogenic callus. The seedling rate and rooting number of seedlings induced by different concentrations of FD were significantly different. Research highlights: This study may aid in the rapid maturation of C. lanceolata somatic embryos and is useful for accelerated C. lanceolata breeding. Keywords: C. lanceolata; Formamide Deionized; Somatic embryogenesis; Seedling rate. Abbreviations used: FD (Formamide Deionized), FD0 (the concentration of 0 g/L FD), FD0.01134 (the concentration of 0.01134 g/L FD), FD0.1134 (the concentration of 0.1134 g/L FD), FD1.134 (the concentration of 1.134 g/L FD), FD11.34 (the concentration of 11.34 g/L FD)

Genome-wide analysis of the GRAS gene family in Liriodendron chinense reveals the putative function in abiotic stress and plant development

IntroductionGRAS genes encode plant-specific transcription factors that play essential roles in plant growth and development. However, the members and the function of the GRAS gene family have not been reported in Liriodendron chinense. L. chinense, a tree species in the Magnolia family that produces excellent timber for daily life and industry. In addition, it is a good relict species for plant evolution research.MethodsTherefore, we conducted a genome-wide study of the LcGRAS gene family and identified 49 LcGRAS genes in L. chinense.ResultsWe found that LcGRAS could be divided into 13 sub-groups, among which there is a unique branch named HAM-t. We carried out RNA sequencing analysis of the somatic embryos from L. chinense and found that LcGRAS genes are mainly expressed after heart-stage embryo development, suggesting that LcGRAS may have a function during somatic embryogenesis. We also investigated whether GRAS genes are responsive to stress by carrying out RNA sequencing (RNA-seq) analysis, and we found that the genes in the PAT subfamily were activated upon stress treatment, suggesting that these genes may help plants survive stressful environments. We found that PIF was downregulated and COR was upregulated after the transient overexpression of PATs, suggesting that PAT may be upstream regulators of cold stress. DiscussionCollectively, LcGRAS genes are conserved and play essential roles in plant development and adaptation to abiotic stress

Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress

The calcineurin B-like (CBL)-interacting protein kinases (CIPKs), a type of plant-specific genes in the calcium signaling pathway, function in response to adverse environments. However, few halophyte derived CIPKs have been studied for their role in plant physiological and developmental adaptation during abiotic stresses, which inhibits the potential application of these genes to improve environmental adaptability of glycophytes. In this study, we constructed Nitraria billardieri CIPK25 overexpressing Arabidopsis and analyzed the seedling development under salt treatment. Our results show that Arabidopsis with NbCIPK25 expression exhibits more vigorous growth than wild type plants under salt condition. To gain insight into the molecular mechanisms underlying salt tolerance, we profiled the transcriptome of WT and transgenic plants via RNA-seq. GO and KEGG analyses revealed that upregulated genes in NbCIPK25 overexpressing seedlings under salt stress are enriched in photosynthesis related terms; Calvin-cycle genes including glyceraldehyde-3-phosphate dehydrogenases (GAPDHs) are significantly upregulated in transgenic plants, which is consistent with a decreased level of NADPH (GAPDH substrate) and increased level of NADP+. Accordingly, NbCIPK25 overexpressing plants exhibited more efficient photosynthesis; soluble sugar and proteins, as photosynthesis products, showed a higher accumulation in transgenic plants. These results provide molecular insight into how NbCIPK25 promotes the expression of genes involved in photosynthesis, thereby maintaining plant growth under salt stress. Our finding supports the potential application of halophyte-derived NbCIPK25 in genetic modification for better salt adaptation

A multi-objective genetic type-2 fuzzy logic based system for mobile field workforce area optimization

In industries which employ large numbers of mobile field engineers (resources), there is a need to optimize the task allocation process. This particularly applies to utility companies such as electricity, gas and water suppliers as well as telecommunications. The process of allocating tasks to engineers involves finding the optimum area for each engineer to operate within where the locations available to the engineers depends on the work area she/he is assigned to. This particular process is termed as work area optimization and it is a sub-domain of workforce optimization. The optimization of resource scheduling, specifically the work area in this instance, in large businesses can have a noticeable impact on business costs, revenues and customer satisfaction. In previous attempts to tackle workforce optimization in real world scenarios, single objective optimization algorithms employing crisp logic were employed. The problem is that there are usually many objectives that need to be satisfied and hence multi-objective based optimization methods will be more suitable. Type-2 fuzzy logic systems could also be employed as they are able to handle the high level of uncertainties associated with the dynamic and changing real world workforce optimization and scheduling problems. This paper presents a novel multi-objective genetic type-2 fuzzy logic based system for mobile field workforce area optimization, which was employed in real world scheduling problems. This system had to overcome challenges, like how working areas were constructed, how teams were generated for each new area and how to realistically evaluate the newly suggested working areas. These problems were overcome by a novel neighborhood based clustering algorithm, sorting team members by skill, location and effect, and by creating an evaluation simulation that could accurately assess working areas by simulating one day's worth of work, for each engineer in the working area, while taking into account uncertainties. The results show strong improvements when the proposed system was applied to the work area optimization problem, compared to the heuristic or type-1 single objective optimization of the work area. Such optimization improvements of the working areas will result in better utilization of the mobile field workforce in utilities and telecommunications companies

Noticiário

In an attempt to achieve the selective oxidation of NOx, a hybrid catalyst of single-atom-anchored metal organic frameworks (MOF, NH2-UiO-66) and MnO2 was constructed and used in the plasma catalytic process. Isolated Ru sites were successfully implanted into the structure of the MOF by simply stirring the mixed liquor containing both MOF and RuCl3, facilitating plasma discharge, NO/NO2 adsorption, and formation of âOH radicals. A special oxo-bridged Zr4+-O-Ru3+ was constructed to accelerate electron transfer and continuous proceeding of the reaction. Directional migration of generated electrons from MOF to Ru sites was witnessed when MOF was activated by plasma-induced "pseudo-photocatalysis". The total (100%) selective plasma-catalytic oxidation of NOx to NO2- and NO3- was achieved at an SIE of 75.3 J/L. The byproduct O3 was effectively degraded and utilized by MnO2, facilitating the deep oxidation of NOx. The facile realization of single atoms would be an ideal way to produce MOF-based catalysts with desired performance. Efficiently combining plasma with single atom-decorated MOF catalysts can provide additional prospects for the plasma-catalytic system. </p