Glucosyl anthranilate

In the crystal structure of the title compound, C21H25NO11, the hexopyranosyl ring adopts a chair conformation and the five substituents are in equatorial positions. An intra­molecular hydrogen bond between the amino group and a neighbouring carbonyl group is found. Two carbonyl groups are disordered and were refined using a split model

Human-derived IgG level as an indicator for EBV-associated lymphoma model in Hu-PBL/SCID chimeras

<p>Abstract</p> <p>Background</p> <p>Epstein-Barr virus (EBV) has a close association with various types of human lymphomas. Animal models are essential to elucidate the pathogenesis of human EBV-associated lymphomas. The aim of the present study is to evaluate the association between human IgG concentration and EBV-associated lymphoma development in huPBL/SCID mice.</p> <p>Methods</p> <p>Human peripheral blood lymphocytes (hu-PBL) from EBV-seropositive donors were inoculated intraperitoneally into SCID mouse. Immunohistochemical staining was used to examine differentiated antigens of tumor cells. EBV infection of the induced tumors was detected by <it>in situ </it>hybridization. IgG concentrations in the serums of 12 SCID mice were measured by unidirectional immunodiffusion assay.</p> <p>Results</p> <p>21 out of 29 mice developed tumors in their body. Immunohistochemical staining showed that all induced tumors were LCA (leukocyte common antigen) positive, B-cell markers (CD20, CD79a) positive, and T-cell markers (both CD3 and CD45RO) negative. The tumors can be diagnosed as human B-cell lymphomas by these morphological and immunohistochemical features. In situ hybridization exhibited resultant tumor cells had EBV encoded small RNA-1 (EBER-1). Human-derived IgG could be found in the serum from SCID mice on the 15^thday following hu-PBL transplantation, and IgG levels increased with the tumor development in 6 hu-PBL/SCID chimeras.</p> <p>Conclusions</p> <p>Intraperitoneal transfer of hu-PBLs from EBV+ donors to SCID mice leads to high human IgG levels in mouse serum and B cell lymphomas. Our findings suggest that increasing levels of human-derived IgG in peripheral blood from hu-PBL/SCID mice could be used to monitor EBV-related human B-cell lymphoma development in experimental animals.</p

DUAL EFFECTS OF BILIRUBIN ON THE PROLIFERATION OF RAT RENAL NRK52E CELLS AND ITS ASSOCIATION WITH GAP JUNCTIONS

Objective: The effect of bilirubin on renal pathophysiology is controversial. This study aimed to observe the effects of bilirubin on the proliferation of normal rat renal tubular epithelial cell line (NRK52E) and its potential interplay with gap junction function. Methods: Cultured NRK52E cells, seeded respectively at high- or low- densities, were treated with varying concentrations of bilirubin for 24 hours. Cell injury was assessed by measuring cell viability and proliferation, and gap junction function was assessed by Parachute dye-coupling assay. Connexin 43 protein was assessed by Western blotting. Results: At doses from 17.1 to 513μmol/L, bilirubin dose-dependently enhanced cell viability and colony-formation rates when cells were seeded at either high- or low- densities (all p\u3c0.05 vs. solvent group) accompanied with enhanced intercellular fluorescence transmission and increased Cx43 protein expression in high-density cells. However, the above effects of BR were gradually reversed when its concentration increased from 684 to 1026μmol/L. In high-density cells, gap junction inhibitor 12-O-tetradecanoylphorbol 13- acetate attenuated bilirubin-induced enhancement of colony-formation and fluorescence transmission. However, in the presence of high concentration bilirubin (1026μmol/L), activation of gap junction with retinoid acid decreased colony-formation rates. Conclusion: Bilirubin can confer biphasic effects on renal NRK52E cell proliferation potentially by differentially affecting gap junction functions

Line identification of extreme ultraviolet spectra from aluminum ions in EAST Tokamak plasmas

Extreme ultraviolet (EUV) spectra emitted from aluminum in the 5-340 A wavelength range were observed in Experimental Advanced Superconducting Tokamak (EAST) discharges. Several spectral lines from aluminum ions with different degrees of ionization were successfully observed with sufficient spectral intensities and resolutions using three fast-time-response EUV spectrometers. The line identification uses three independent state-of-art computational codes for the atomic structure calculations, which provide the wavelengths and radiative transition probabilities rate coefficients. These programs are HULLAC (Hebrew University - Lawrence Livermore Atomic Code), AUTOSTRUCTURE, and FAC (Flexible Atomic Code). Using three different codes allows us to resolve some ambiguities in identifying certain spectral lines and assess the validity of the theoretical predictions

Rickettsial Seroepidemiology among Farm Workers, Tianjin, People’s Republic of China

High seroprevalence rates for Anaplasma phagocytophilum (8.8%), Coxiella burnetii (6.4%), Bartonella henselae (9.6%), and Rickettsia typhi (4.1%) in 365 farm workers near Tianjin, People’s Republic of China, suggest that human infections with these zoonotic bacteria are frequent and largely unrecognized. Demographic features of seropositive persons suggest distinct epidemiology, ecology, and risks

Study on the Moderating Effect of Body Mass Index in Correlation of Anxiety and Depression Disorders

BackgroundAs two different kinds of mental disorders, anxiety disorder and depressive disorder could probably coexist in one with the proceeding of the illness. However, there are few studies on how to prevent and treat the coexistence of anxiety and depressive disorders.ObjectiveTo discuss the relationship between anxiety and depressive disorders, and the moderating effect of body mass index (BMI) in their relationship.MethodsBy use of simple random sampling, 86 outpatients and inpatients with anxiety disorders were selected from the First Affiliated Hospital of Nanchang University during June 1st to August, 31st, 2021. A self-made demographic questionnaire was used to obtain the demographic information. The Hamilton Anxiety Rating Scale was used to assess the anxiety level. The Hamilton Rating Scale for Depression was used to assess the depression level. Pearson correlation analyses were performed to assess the relationship between anxiety and depression disorders, and that between BMI and anxiety or depression disorder. Hierarchical regression analysis was adopted to explore the moderating effect of BMI on the relationship between anxiety and depressive disorders.ResultsEighty eligible cases were also diagnosed with depression. The anxiety prevalence was significantly increased with depression prevalence (r=0.70, P&lt;0.01) . BMI had no significant linear correlation with anxiety prevalence (r=0.03, P&gt;0.05) . BMI also had no significant linear correlation with depression prevalence (r=0.14, P&gt;0.05) . BMI moderated the relationship between anxiety and depression disorders significantly (&#x03B2;=-0.16, P&lt;0.01) .ConclusionBMI can weaken the effect of anxiety disorder on depression, helping prevent them from developing into comorbid mental disorders, providing new ideas for developing new dietary standard or exercise patterns for mental health prevention and treatment in the future and expands the research field of nutritional psychiatry to a certain extent

Psychometric properties of the Depression Stigma Scale (DSS) in Chinese cancer patients:a cross-sectional study

Objectives The Depression Stigma Scale (DSS) is commonly used to assess depression stigma in the general population and in people with depression. The DSS includes two 9-item subscales assumed to measure personal depression stigma (ie, personal perceptions of depression) and perceived depression stigma (ie, perceptions of how others perceive depression). The aim of the present study was to examine its psychometric properties in terms of validity and reliability in Chinese cancer patients. Design A cross-sectional study design. Participants and settings This study focused on 301 Chinese cancer patients recruited from two hospitals in Xian, China. Methods Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were used to assess the factor structure. Internal consistency was assessed using Cronbachs alpha. To examine concurrent validity, symptoms of depression were used as the criterion. Results For each subscale of the DSS (ie, personal and perceived depression stigma), the EFA and CFA confirmed a two-factor structure: weak-not-sick (ie, perceiving that depression is not a real illness, but rather a sign of weakness) and discrimination (ie, perceiving that depressed people are discriminated against). The Cronbachs alphas were adequate, ranging from 0.70 to 0.80. Symptoms of depression were positively but weakly correlated to personal and perceived depression stigma. Conclusions The DSS appeared to show satisfactory psychometric properties in our sample of cancer patients. Both personal depression stigma and perceived depression stigma subscales consisted of two underlying aspects

Phosphoproteomics Screen Reveals Akt Isoform-Specific Signals Linking RNA Processing to Lung Cancer

The three Akt isoforms are functionally distinct. Here we show that their phosphoproteomes also differ, suggesting that their functional differences are due to differences in target specificity. One of the top cellular functions differentially regulated by Akt isoforms is RNA processing. IWS1, an RNA processing regulator, is phosphorylated by Akt3 and Akt1 at Ser720/Thr721. The latter is required for the recruitment of SETD2 to the RNA Pol II complex. SETD2 trimethylates histone H3 at K36 during transcription, creating a docking site for MRG15 and PTB. H3K36me3-bound MRG15 and PTB regulate FGFR-2 splicing, which controls tumor growth and invasiveness downstream of IWS1 phosphorylation. Twenty-one of the twenty-four non-small-cell-lung carcinomas we analyzed express IWS1. More importantly, the stoichiometry of IWS1 phosphorylation in these tumors correlates with the FGFR-2 splicing pattern and with Akt phosphorylation and Akt3 expression. These data identify an Akt isoform-dependent regulatory mechanism for RNA processing and demonstrate its role in lung cancer

Patterns of unmet supportive needs and relationship to quality of life in Chinese cancer patients

ObjectiveThis study aimed to (1) identify distinct patterns of unmet needs in Chinese cancer patients; (2) examine whether sociodemographic and medical characteristics distinguished these patterns; and (3) examine whether people with distinct patterns reported differential quality of life (QoL). MethodsThis cross-sectional study recruited 301 cancer patients from 2 hospitals in China. The 34-item Supportive Care Needs Survey Short-Form was used to measure unmet needs across 5 domains: physical and daily living, psychological, patient care and support, health systems and information, and sexuality. Latent class analysis was performed to identify patterns of unmet needs across these domains. ResultsFour patterns of unmet needs were identified, differing in levels and nature of unmet needs. Participants in class 1 (47%) reported few unmet needs. Patients in class 2 (15%) had moderate levels of unmet needs, displaying similar levels across 5 domains. People in class 3 (25%) and class 4 (13%) reported similarly high levels on psychological, health care system and information, physical and daily living, and patient care, but differing in sexuality, with class 3 reporting low levels while class 4 high on sexuality. None of sociodemographic and medical characteristics distinguished these patterns significantly. Compared to other classes, people in class 1 reported highest levels of QoL. ConclusionsThis study demonstrates the existence of 4 patterns of unmet supportive needs in Chinese cancer patients. Patients with few unmet needs reported the best QoL

Spread of Streptococcus suis Sequence Type 7, China

Streptococcus suis sequence type (ST) 7 has been spreading throughout China. To determine events associated with its emergence, we tested 114 isolates. In all 106 ST7 strains responsible for human outbreaks and sporadic infections, the tetracycline-resistance gene, tetM, was detected on the conjugative transposon Tn916. Horizontal transmission of tetM is suspected