Planning Emergency Shelters for Urban Disasters: A Multi-Level Location–Allocation Modeling Approach

In recent years, cities are threatened by various natural hazards. Planning emergency shelters in advance is an effective approach to reducing the damage caused by disasters and ensuring the safety of residents. Thus, providing the optimal layout of urban emergency shelters is an important stage of disaster management and an act of humanitarian logistics. In order to study the optimal layout of emergency shelters in small mountain cities, this paper constructs multi-level location models for different grades of emergency shelters so as to minimize the travel and construction costs and maximize the coverage rate. Specifically, the actual service of emergency shelters is determined using Geographic Information System (GIS) software and Weighted Voronoi Diagram (WVD) models under the limitation of site capacity, and the space layout is adjusted through combining the actual urban land with the construction position. In this paper, the Jianchuan county seat at Yunnan Province, China, was considered as a case study to illustrate the models of emergency shelters in which the feasibility of the presented models is verified. The proposed research methods and models have provided theoretical basis and a benchmark for the optimal layout of emergency shelters in other small mountain cities

An Improved Modeling for Low-grade Organic Rankine Cycle Coupled with Optimization Design of Radial-inflow Turbine

This document is the Accepted Manuscript of the following article: Lijing Zhai, Guoqiang Xu, Jie Wen, Yongkai Quan, Jian Fu, Hongwei Wu, and Tingting Li, ‘An improved modeling for low-grade organic Rankine cycle coupled with optimization design of radial-inflow turbine’, Energy Conversion and Management, Vol. 153: 60-70, December 2017. Under embargo. Embargo end date: 10 October 2018. The final, published version is available online at DOI: https://doi.org/10.1016/j.enconman.2017.09.063. Published by Elsevier Ltd.Organic Rankine cycle (ORC) has been proven to be an effective and promising technology to convert low-grade heat energy into power, attracting rapidly growing interest in recent years. As the key component of the ORC system, turbine significantly influences the overall cycle performance and its efficiency also varies with different working fluids as well as in different operating conditions. However, turbine efficiency is generally assumed to be constant in the conventional cycle design. Aiming at this issue, this paper couples the ORC system design with the radial-inflow turbine design to investigate the thermodynamic performance of the ORC system and the aerodynamic characteristics of radial-inflow turbine simultaneously. The constrained genetic algorithm (GA) is used to optimize the radial-inflow turbine with attention to six design parameters, including degree of reaction, velocity ratio, loading coefficient, flow coefficient, ratio of wheel diameter, and rotational speed. The influence of heat source outlet temperature on the performance of the radial-inflow turbine and the ORC system with constant mass flow rate of the heat source and constant heat source inlet temperature is investigated for four kinds of working fluids. The net electrical powers achieved are from few tens kWs to one hundred kWs. The results show that the turbine efficiency decreases with increasing heat source outlet temperature and that the decreasing rate of turbine efficiency becomes faster in the high temperature region. The optimized turbine efficiency varies from 88.06% (using pentane at the outlet temperature of 105 ºC) to 91.01% (using R245fa at the outlet temperature of 80 ºC), which appears much higher compared to common values reported in the literature. Furthermore, the cycle efficiency increases monotonously with the growth of the heat source outlet temperature, whereas the net power output has the opposite trend. R123 achieves the maximum cycle efficiency of 12.21% at the heat source outlet temperature of 110 ºC. Based on the optimized results, the recommended ranges of the key design parameters for ORC radial-inflow turbine are presented as well.Peer reviewe

Dynamic changes and convergence of China’s regional green productivity:A dynamic spatial econometric analysis

Low-carbon economic development is at the heart of the post-pandemic green recovery scheme worldwide. It requires economic recovery without compromising on the environment, implying a critical role that green productivity plays in achieving the carbon neutrality goal. Green productivity measures the quality of economic growth with consideration for energy consumption and environmental pollution. This study employs the slacks-based measure directional distance function (SBM-DDF) approach and the Malmquist-Luenberger (ML) index to calculate green productivity and its components of 30 provinces in China between 2001 and 2018. Using a spatial panel data model, we empirically analyzed the conditional β-convergence of China's green productivity. We found that overall, since 2001, China's green productivity has demonstrated a continuous upward trend. When taking into account spatial factors, China's green productivity demonstrates a significant conditional β-convergence. In terms of regional effects, the results indicate that the green productivity of the eastern and western regions demonstrates club convergence, implying a more balanced green economic development. Moreover, the convergence rate of China's green productivity increases with the addition of environmental regulation variable, and so the corresponding convergence time decreases. It indicates that environmental regulations help to facilitate the convergence of China's green productivity, narrowing the gap between the regional green economic development. The findings provide guideline for achieving a low-carbon development and carbon neutrality from a regional green productivity perspective

Effect of Dihuang rougui decoction on ovariectomyinduced osteoporosis in rats

Purpose: To investigate the effect of Dihuang Rougui Decoction (DRD) on ovariectomy-induced osteoporosis in rats.Methods: Female Sprague-Dawley rats were randomly assigned to a  normal group (control) and five ovariectomy (OVX) subgroups: OVX with vehicle (OVX), OVX with positive control drug (alendronate sodium tablets, 1.6 mg/kg/week), and OVX + DRD (75, 150 or 300 mg/kg/day). After the rats were subjected to ovariectomy for 4 weeks, fosamax or DRD were administered daily (orally) for 16 weeks. The bone mineral density (BMD) of the L4 vertebrae and right femurs of the rats was evaluated. Serum hormones estradiol (E2), follicle-stimulating  hormone (FSH) and luteinizing hormone (LH) levels and serum alkaline phosphatase (ALP), osteocalcin (OC) and telopeptides of collagen type I (CTx) levels of the rats were determined. The bone tissue morphology of the rats was examined by microscopy.Results: The results show that DRD dose-dependently inhibited bone  mineral density (BMD) reduction of L4 vertebrae and femurs (both p < 0.05). DRD significantly increased serum E2, FSH and LH levels (p < 0.05) in the osteoporotic rats, and significantly lowered serum ALP, CTx and OC concentrations, compared to OVX group (p < 0.01). Compared with OVX model group, bone trabeculae in all three DRD groups and nilestriol groups were wider, and the space and connections markedly increased. Furthermore, the medullary cavity reduced in size.Conclusion: These findings indicate that DRD mitigates OVX-induced  osteoporosis in rats, and thus, the decoction has a potential for clinical  management of osteoporosis patients.Keywords: Dihuang Rougui decoction, Osteoporosis, Ovariectomy, Bone mineral density, Serum bone marker, Bone tissue morpholog

CRISPR/Cas9-mediated targeted mutation of the E1 decreases photoperiod sensitivity, alters stem growth habits, and decreases branch number in soybean

The distribution of elite soybean (Glycine max) cultivars is limited due to their highly sensitive to photoperiod, which affects the flowering time and plant architecture. The recent emergence of CRISPR/Cas9 technology has uncovered new opportunities for genetic manipulation of soybean. The major maturity gene E1 of soybean plays a critical role in soybean photoperiod response. Here, we performed CRISPR/Cas9-mediated targeted mutation of E1 gene in soybean cultivar Tianlong1 carrying the dominant E1 to investigate its precise function in photoperiod regulation, especially in plant architecture regulation. Four types of mutations in the E1 coding region were generated. No off-target effects were observed, and homozygous trans-clean mutants without T-DNA were obtained. The photoperiod sensitivity of e1 mutants decreased relative to the wild type plants; however, e1 mutants still responded to photoperiod. Further analysis revealed that the homologs of E1, E1-La, and E1-Lb, were up-regulated in the e1 mutants, indicating a genetic compensation response of E1 and its homologs. The e1 mutants exhibited significant changes in the architecture, including initiation of terminal flowering, formation of determinate stems, and decreased branch numbers. To identify E1-regulated genes related to plant architecture, transcriptome deep sequencing (RNA-seq) was used to compare the gene expression profiles in the stem tip of the wild-type soybean cultivar and the e1 mutants. The expression of shoot identity gene Dt1 was significantly decreased, while Dt2 was significantly upregulated. Also, a set of MADS-box genes was up-regulated in the stem tip of e1 mutants which might contribute to the determinate stem growth habit

Numerical analysis of the axial heat conduction with variable fluid properties in a forced laminar flow tube

This document is the Accepted Manuscript version of the following article: Lijing Zhai, et al, ‘Numerical analysis of the axial heat conduction with variable fluid properties in a forced laminar flow tube’, International Journal of Heat and Mass Transfer, Vol. 114: 238-251, November 2017. Under embargo until 22 June 2018. The final, definitive version is available online at doi: https://doi.org/10.1016/j.ijheatmasstransfer.2017.06.041.In this article, a theoretical model is developed to investigate the effects of the axial heat conduction on the laminar forced convection in a circular tube with uniform internal heat generation in the solid wall. In the current work, three different fluids, i.e. water, n-decane and air, are selected on purpose since their thermophysical properties show different behavior with temperature. The effects of the axial heat conduction with varying dynamic viscosity and/or varying thermal conductivity are investigated in a systematic manner. Results indicate that the variable-property effects could alleviate the reduction in Nusselt number (Nu) due to the axial heat conduction. For the case of Peclet number (Pe) equal to 100, wall thickness to inner diameter ratio of 1 and solid wall to fluid thermal conductivity ratio of 100, the maximum Nu deviation between constant and variable properties are up to 7.33% at the entrance part for water in the temperature range of 50℃, and 4.45% at the entrance part for n-decane in the temperature range of 120℃, as well as 2.20% at the ending part for air in the temperature range of 475℃, respectively. In addition, the average Nu deviation for water, n-decane and air are 3.24%, 1.94% and 1.74%, respectively. Besides, Nu decreases drastically with decreasing Pe when Pe≤500 and with increasing solid wall to fluid thermal conductivity ratio ( ) when ≤100. It is also found that variable properties have more obvious effects on the velocity profile at the upstream part while more obvious effects on the temperature profile at the downstream part.Peer reviewe

TAF1B depletion leads to apoptotic cell death by inducing nucleolar stress and activating p53-miR-101 circuit in hepatocellular carcinoma

BackgroundTAF1B (TATA Box Binding Protein (TBP)-Associated Factor) is an RNA polymerase regulating rDNA activity, stress response, and cell cycle. However, the function of TAF1B in the progression of hepatocellular carcinoma (HCC) is unknown.ObjectiveIn this study, we intended to characterize the crucial role and molecular mechanisms of TAF1B in modulating nucleolar stress in HCC.MethodsWe analyzed the differential expression and prognostic value of TAF1B in hepatocellular carcinoma based on The Cancer Genome Atlas (TCGA) database, tumor and paraneoplastic tissue samples from clinical hepatocellular carcinoma patients, and typical hepatocellular carcinoma. We detected cell proliferation and apoptosis by lentiviral knockdown of TAF1B expression levels in HepG2 and SMMC-7721 cells using clone formation, apoptosis, and Western blotting (WB) detection of apoptosis marker proteins. Simultaneously, we investigated the influence of TAF1B knockdown on the function of the pre-initiation complex (PIC) by WB, and co-immunoprecipitation (Co-IP) and chromatin immunoprecipitation (ChIP) assays verified the interaction between the complexes and the effect on rDNA activity. Immunofluorescence assays measured the expression of marker proteins of nucleolus stress, fluorescence in situ hybridization (FISH) assays checked the rDNA activity, and qRT-PCR assays tested the pre-rRNA levels. Regarding molecular mechanisms, we investigated the role of p53 and miR-101 in modulating nucleolar stress and apoptosis. Finally, the impact of TAF1B knockdown on tumor growth, apoptosis, and p53 expression was observed in xenograft tumors.ResultWe identified that TAF1B was highly expressed in hepatocellular carcinoma and associated with poor prognosis in HCC patients. TAF1B depletion modulated nucleolar stress and apoptosis in hepatocellular carcinoma cells through positive and negative feedback from p53-miR-101. RNA polymerase I transcription repression triggered post-transcriptional activation of miR-101 in a p53-dependent manner. In turn, miR-101 negatively feeds back through direct inhibition of the p53-mediated PARP pathway.ConclusionThese findings broaden our comprehension of the function of TAF1B-mediated nucleolar stress in hepatocellular carcinoma and may offer new biomarkers for exploring prospective therapeutic targets in HCC

Zoonotic Viruses Associated with Illegally Imported Wildlife Products

The global trade in wildlife has historically contributed to the emergence and spread of infectious diseases. The United States is the world's largest importer of wildlife and wildlife products, yet minimal pathogen surveillance has precluded assessment of the health risks posed by this practice. This report details the findings of a pilot project to establish surveillance methodology for zoonotic agents in confiscated wildlife products. Initial findings from samples collected at several international airports identified parts originating from nonhuman primate (NHP) and rodent species, including baboon, chimpanzee, mangabey, guenon, green monkey, cane rat and rat. Pathogen screening identified retroviruses (simian foamy virus) and/or herpesviruses (cytomegalovirus and lymphocryptovirus) in the NHP samples. These results are the first demonstration that illegal bushmeat importation into the United States could act as a conduit for pathogen spread, and suggest that implementation of disease surveillance of the wildlife trade will help facilitate prevention of disease emergence