518 research outputs found
Generalized Hyper-Ramsey Resonance with separated oscillating fields
An exact generalization of the Ramsey transition probability is derived to
improve ultra-high precision measurement and quantum state engineering when a
particle is subjected to independently-tailored separated oscillating fields.
The phase-shift accumulated at the end of the interrogation scheme offering
high-level control of quantum states throughout various laser parameters
conditions. The Generalized Hyper-Ramsey Resonance based on independent
manipulation of interaction time, field amplitude, phase and frequency detuning
is presented to increase the performance of next generation of atomic,
molecular and nuclear clocks, to upgrade high resolution frequency measurement
in Penning trap mass spectrometry and for a better control of light induced
frequency shifts in matter wave interferometers or quantum information
processing.Comment: accepted for publication in Phys. Rev.
Composite pulses in Hyper-Ramsey spectroscopy for the next generation of atomic clocks
The next generation of atomic frequency standards based on an ensemble of
neutral atoms or a single-ion will provide very stringent tests in metrology,
applied and fundamental physics requiring a new step in very precise control of
external systematic corrections. In the proceedings of the 8th Symposium on
Frequency Standards and Metrology, we present a generalization of the recent
Hyper-Ramsey spectroscopy with separated oscillating fields using composites
pulses in order to suppress field frequency shifts induced by the interrogation
laser itself. Sequences of laser pulses including specific selection of phases,
frequency detunings and durations are elaborated to generate spectroscopic
signals with a strong reduction of the light-shift perturbation by off resonant
states. New optical clocks based on weakly allowed or completely forbidden
transitions in atoms, ions, molecules and nuclei will benefit from these
generalized Ramsey schemes to reach relative accuracies well below the
10 level.Comment: accepted as proceedings of the 8th Symposium on Frequency Standards
and Metrology (Potsdam Germany, 12-16 october 2015
Synthetic Frequency Protocol in the Ramsey Spectroscopy of Clock Transitions
We develop an universal method to significantly suppress probe-induced shifts
in any types of atomic clocks using the Ramsey spectroscopy. Our approach is
based on adaptation of the synthetic frequency concept [V. I. Yudin, et al.,
Phys. Rev. Lett. 107, 030801 (2011)] (previously developed for BBR shift
suppression) to the Ramsey spectroscopy with the use of interrogations for
different dark time intervals. Universality of the method consists in
arbitrariness of the possible Ramsey schemes. However, most extremal results
are obtained in combination with so-called hyper-Ramsey spectroscopy [V. I.
Yudin, et al., Phys. Rev. A 82, 011804(R) (2010)]. In the latter case, the
probe-induced frequency shifts can be suppressed considerably below a
fractional level of 10 practically for any optical atomic clocks, where
this shift previously was metrologically significant. The main advantage of our
method in comparison with other radical hyper-Ramsey approaches [R. Hobson, et
al., Phys. Rev. A 93, 010501(R) (2016); T. Zanon-Willette, et al., Phys. Rev. A
93, 042506 (2016)] consist in much greater efficiency and resistibility in the
presence of decoherentization.Comment: 9 pages, 7 figure
Influência de reguladores de crescimento e adubação no florescimento e crescimento de Eucalyptus dunnii Maid.
A influência de reguladores de crescimento e da adubação sobre o florescimento e crescimento de Eucalyptus dunnii Maid., de cinco anos de idade, foi determinada em árvores selecionadas de um talhão experimental localizado no Centro Nacional de Pesquisa de Florestas/EMBRAPA, PR. Neste, foram realizadas aplicações de ácido giberélico (GA3) na concentração de 300 mg. l -1 , cinetina a 50 mg. l -1 , combinação das concentrações de GA3 + cinetina, Ethrel a 240 mg. l -1 e 400 g de 10-30-15 (NPK) mais 10 g de micronutrientes. O florescimento não foi influenciado pelos tratamentos empregados. Entretanto, para o crescimento, verificou-se que os maiores incrementos para altura e diâmetro foram obtidos com a aplicação de GA3 + cinetina. A cinetina, isoladamente, teve um efeito negativo, enquanto que a adubação não trouxe acréscimo significativo no crescimento de E. dunnii. Com base nesses resultados, sugere-se que estudos posteriores, envolvendo outros tratamentos, sejam realizados para o estabelecimento da técnica de indução do florescimento nessa espécie
Identification of specific molecular structures of human immunodeficiency virus type 1 Tat relevant for its biological effects on vascular endothelial cells.
Human immunodeficiency virus type 1 (HIV-1) Tat transactivates viral genes and is released by infected cells, acting as a soluble mediator. In endothelial cells (EC), it activates a proangiogenic program by activating vascular endothelial growth factor receptor type 2 (VEGFR-2) and integrins. A structure-activity relationship study was performed by functional analysis of Tat substitution and deletion variants to define the Tat determinants necessary for EC activation. Variants were made (i) in the basic and (ii) in the cysteine-rich domains and (iii) in the C-terminal region containing the RGD sequence required for integrin recognition. Our results led to the following conclusions. (i) Besides a high-affinity binding site corresponding to VEGFR-2, EC express low-affinity binding sites. (ii) The basic and the cysteine-rich variants bind only to the low-affinity binding sites and do not promote tyrosine phosphorylation of VEGFR-2. Furthermore, they have a reduced ability to activate EC in vitro, and they lack angiogenic activity. (iii) Mutants with mutations in the C-terminal region are partially defective for in vitro biological activities and in vivo angiogenesis, but they activate VEGFR-2 as Tat wild type. In conclusion, regions encoded by the first exon of tat are necessary and sufficient for activation of VEGFR-2. However, the C-terminal region, most probably through RGD-mediated integrin engagement, is indispensable for full activation of an in vitro and in vivo angiogenic progra
Model Predictive Control of Nonholonomic Mobile Robots Without Stabilizing Constraints and Costs
A new FSA approach for in situ -ray spectroscopy
An increasing demand of environmental radioactivity monitoring comes both
from the scientific community and from the society. This requires accurate,
reliable and fast response preferably from portable radiation detectors. Thanks
to recent improvements in the technology, -spectroscopy with sodium
iodide scintillators has been proved to be an excellent tool for in-situ
measurements for the identification and quantitative determination of
-ray emitting radioisotopes, reducing time and costs. Both for
geological and civil purposes not only K, U, and Th have
to be measured, but there is also a growing interest to determine the
abundances of anthropic elements, like Cs and I, which are used
to monitor the effect of nuclear accidents or other human activities.
The Full Spectrum Analysis (FSA) approach has been chosen to analyze the
-spectra. The Non Negative Least Square (NNLS) and the energy
calibration adjustment have been implemented in this method for the first time
in order to correct the intrinsic problem related with the
minimization which could lead to artifacts and non physical results in the
analysis.
A new calibration procedure has been developed for the FSA method by using in
situ -spectra instead of calibration pad spectra. Finally, the new
method has been validated by acquiring -spectra with a 10.16 cm x 10.16
cm sodium iodide detector in 80 different sites in the Ombrone basin, in
Tuscany. The results from the FSA method have been compared with the laboratory
measurements by using HPGe detectors on soil samples collected in the different
sites, showing a satisfactory agreement between them. In particular, the
Cs isotopes has been implemented in the analysis since it has been
found not negligible during the in-situ measurements.Comment: accepted by Science of Total Environment: 8 pages, 10 figures, 3
table
Differential expression of microRNAs in mouse pain models
<p>Abstract</p> <p>Background</p> <p>MicroRNAs (miRNAs) are short non-coding RNAs that inhibit translation of target genes by binding to their mRNAs. The expression of numerous brain-specific miRNAs with a high degree of temporal and spatial specificity suggests that miRNAs play an important role in gene regulation in health and disease. Here we investigate the time course gene expression profile of miR-1, -16, and -206 in mouse dorsal root ganglion (DRG), and spinal cord dorsal horn under inflammatory and neuropathic pain conditions as well as following acute noxious stimulation.</p> <p>Results</p> <p>Quantitative real-time polymerase chain reaction analyses showed that the mature form of miR-1, -16 and -206, is expressed in DRG and the dorsal horn of the spinal cord. Moreover, CFA-induced inflammation significantly reduced miRs-1 and -16 expression in DRG whereas miR-206 was downregulated in a time dependent manner. Conversely, in the spinal dorsal horn all three miRNAs monitored were upregulated. After sciatic nerve partial ligation, miR-1 and -206 were downregulated in DRG with no change in the spinal dorsal horn. On the other hand, axotomy increases the relative expression of miR-1, -16, and 206 in a time-dependent fashion while in the dorsal horn there was a significant downregulation of miR-1. Acute noxious stimulation with capsaicin also increased the expression of miR-1 and -16 in DRG cells but, on the other hand, in the spinal dorsal horn only a high dose of capsaicin was able to downregulate miR-206 expression.</p> <p>Conclusions</p> <p>Our results indicate that miRNAs may participate in the regulatory mechanisms of genes associated with the pathophysiology of chronic pain as well as the nociceptive processing following acute noxious stimulation. We found substantial evidence that miRNAs are differentially regulated in DRG and the dorsal horn of the spinal cord under different pain states. Therefore, miRNA expression in the nociceptive system shows not only temporal and spatial specificity but is also stimulus-dependent.</p
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