523 research outputs found
Colloidal stability of tannins: astringency, wine tasting and beyond
Tannin-tannin and tannin-protein interactions in water-ethanol solvent
mixtures are studied in the context of red wine tasting. While tannin
self-aggregation is relevant for visual aspect of wine tasting (limpidity and
related colloidal phenomena), tannin affinities for salivary proline-rich
proteins is fundamental for a wide spectrum of organoleptic properties related
to astringency. Tannin-tannin interactions are analyzed in water-ethanol
wine-like solvents and the precipitation map is constructed for a typical grape
tannin. The interaction between tannins and human salivary proline-rich
proteins (PRP) are investigated in the framework of the shell model for
micellization, known for describing tannin-induced aggregation of beta-casein.
Tannin-assisted micellization and compaction of proteins observed by SAXS are
described quantitatively and discussed in the case of astringency
d-wave superconductivity and Pomeranchuk instability in the two-dimensional Hubbard model
We present a systematic stability analysis for the two-dimensional Hubbard
model, which is based on a new renormalization group method for interacting
Fermi systems. The flow of effective interactions and susceptibilities confirms
the expected existence of a d-wave pairing instability driven by
antiferromagnetic spin fluctuations. More unexpectedly, we find that strong
forward scattering interactions develop which may lead to a Pomeranchuk
instability breaking the tetragonal symmetry of the Fermi surface.Comment: 4 pages (RevTeX), 4 eps figure
Spontaneous symmetry breaking in the colored Hubbard model
The Hubbard model is reformulated in terms of different ``colored'' fermion
species for the electrons or holes at different lattice sites.
Antiferromagnetic ordering or d-wave superconductivity can then be described in
terms of translationally invariant expectation values for colored composite
scalar fields. A suitable mean field approximation for the two dimensional
colored Hubbard model shows indeed phases with antiferromagnetic ordering or
d-wave superconductivity at low temperature. At low enough temperature the
transition to the antiferromagnetic phase is of first order. The present
formulation also allows an easy extension to more complicated microscopic
interactions.Comment: 19 pages, 5 figure
Exact integral equation for the renormalized Fermi surface
The true Fermi surface of a fermionic many-body system can be viewed as a
fixed point manifold of the renormalization group (RG). Within the framework of
the exact functional RG we show that the fixed point condition implies an exact
integral equation for the counterterm which is needed for a self-consistent
calculation of the Fermi surface. In the simplest approximation, our integral
equation reduces to the self-consistent Hartree-Fock equation for the
counterterm.Comment: 5 pages, 1 figur
Lead bioaccumulation and translocation in herbaceous plants grown in urban and peri-urban soil and the potential human health risk
Exact renormalization group flow equations for non-relativistic fermions: scaling towards the Fermi surface
We construct exact functional renormalization group (RG) flow equations for
non-relativistic fermions in arbitrary dimensions, taking into account not only
mode elimination but also the rescaling of the momenta, frequencies and the
fermionic fields. The complete RG flow of all relevant, marginal and irrelevant
couplings can be described by a system of coupled flow equations for the
irreducible n-point vertices. Introducing suitable dimensionless variables, we
obtain flow equations for generalized scaling functions which are continuous
functions of the flow parameter, even if we consider quantities which are
dominated by momenta close to the Fermi surface, such as the density-density
correlation function at long wavelengths. We also show how the problem of
constructing the renormalized Fermi surface can be reduced to the problem of
finding the RG fixed point of the irreducible two-point vertex at vanishing
momentum and frequency. We argue that only if the degrees of freedom are
properly rescaled it is possible to reach scale-invariant non-Fermi liquid
fixed points within a truncation of the exact RG flow equations.Comment: 20 Revtex pages, with 4 figures; final version to appear in Phys.
Rev. B; references and some explanations adde
Aggregation of antibody drug conjugates at room temperature: SAXS and light scattering evidence for colloidal instability of a specific subpopulation
Coupling an hydrophobic drug onto monoclonal antibodies via Lysine residues is a common route to prepare antibody-drug conjugates (ADC), a promising class of biotherapeutics. But a few chemical modifications on protein surface often increases aggregation propensity, without clear understanding of the aggregation mechanisms at stake (loss of colloidal stability, self- assemblies, denaturation...), and the statistical nature of conjugation introduces polydispersity in the ADC population, which raises questions on whether the whole ADC population becomes unstable. To characterize the average interactions between ADC, we monitored small angle X-ray scattering in solutions of monoclonal IgG1 human antibody drug conjugate, with average degree of conjugation of 0, 2, or 3 drug molecules per protein. To characterize stability, we studied kinetics of aggregation at room temperature. Intrinsic Fuchs stability ratio of the ADC was estimated from the variation over time of scattered light intensity and hydrodynamic radius, in buffers of varying pH, and at diverse sucrose (0% or 10%) and NaCl (0 or 100 mM) concentrations. We show that stable ADC stock solutions became unstable upon pH shift, well below the pH of maximum average attraction between IgGs. Data indicates that aggregation can be ascribed to a fraction of ADC population usually representing less than 30 mol% of the sample. In contrast to the case of (monodisperse) monoclonal antibodies, our results suggest that a poor correlation between stability and average interaction parameters should be expected as a corollary of dispersity of ADC conjugation. In practice, the most unstable fraction of the ADC population can be removed by filtrations, which affects remarkably the apparent stability of the samples. Finally, the lack of correlation between the kinetic stability and variations of the average inter-ADC interactions is tentatively attributed to the uneven nature of charge distributions and the presence of patches on the drug-modified antibodies.This work was supported by the French National Research Agency (program Blanc International, grant ANR 2010-INT 1501, and program Investissement d’Avenir ANR-11- LABX-0011-01, and by SANOFI research grant to BFP. Authors are grateful to Javier Perez and Aurélien Thureau for their help and advice in SAXS measurements at SOLEIL. We thank Sophie Norvez from MMC laboratory in ESPCI for her help with circular dichroism.This is the author accepted manuscript. The final version is available from the American Chemical Society via http://dx.doi.org/10.1021/acs.langmuir.6b0065
Aggregation of Antibody Drug Conjugates at Room Temperature: SAXS and Light Scattering Evidence for Colloidal Instability of a Specific Subpopulation
Coupling a hydrophobic drug onto monoclonal antibodies via lysine residues is a common route to prepare antibody–drug conjugates (ADC), a promising class of biotherapeutics. But a few chemical modifications on protein surface often increase aggregation propensity, without a clear understanding of the aggregation mechanisms at stake (loss of colloidal stability, self-assemblies, denaturation, etc.), and the statistical nature of conjugation introduces polydispersity in the ADC population, which raises questions on whether the whole ADC population becomes unstable. To characterize the average interactions between ADC, we monitored small-angle X-ray scattering in solutions of monoclonal IgG1 human antibody drug conjugate, with average degree of conjugation of 0, 2, or 3 drug molecules per protein. To characterize stability, we studied the kinetics of aggregation at room temperature. The intrinsic Fuchs stability ratio of the ADC was estimated from the variation over time of scattered light intensity and hydrodynamic radius, in buffers of varying pH, and at diverse sucrose (0% or 10%) and NaCl (0 or 100 mM) concentrations. We show that stable ADC stock solutions became unstable upon pH shift, well below the pH of maximum average attraction between IgGs. Data indicate that aggregation can be ascribed to a fraction of ADC population usually representing less than 30 mol % of the sample. In contrast to the case of (monodisperse) monoclonal antibodies, our results suggest that a poor correlation between stability and average interaction parameters should be expected as a corollary of dispersity of ADC conjugation. In practice, the most unstable fraction of the ADC population can be removed by filtration, which affects remarkably the apparent stability of the samples. Finally, the lack of correlation between the kinetic stability and variations of the average inter-ADC interactions is tentatively attributed to the uneven nature of charge distributions and the presence of patches on the drug-modified antibodies
Magnetic and superconducting instabilities of the Hubbard model at the van Hove filling
We use a novel temperature-flow renormalization group technique to analyze
magnetic and superconducting instabilities in the two-dimensional t-t' Hubbard
model for particle densities close to the van Hove filling as a function of the
next-nearest neighbor hopping t'. In the one-loop flow at the van Hove filling,
the characteristic temperature for the flow to strong coupling is suppressed
drastically around t'_c approx. -0.33t, suggesting a quantum critical point
between d-wave pairing at moderate t'>t'_c and ferromagnetism for t'<t'_c. Upon
increasing the particle density in the latter regime the leading instability
occurs in the triplet pairing channel.Comment: 4 pages, to appear in Physical Review Letter
Extreme Mountain Ultra-Marathon Leads to Acute but Transient Increase in Cerebral Water Diffusivity and Plasma Biomarkers Levels Changes.
Background: Pioneer studies demonstrate the impact of extreme sport load on the human brain, leading to threatening conditions for athlete's health such as cerebral edema. The investigation of brain water diffusivity, allowing the measurement of the intercellular water and the assessment of cerebral edema, can give a great contribution to the investigation of the effects of extreme sports on the brain. We therefore assessed the effect of supra-physiological effort (extreme distance and elevation changes) in mountain ultra-marathons (MUMs) athletes combining for the first time brain magnetic resonance imaging (MRI) and blood parameters. Methods:This longitudinal study included 19 volunteers (44.2 ± 9.5 years) finishing a MUM (330 km, elevation + 24000 m). Quantitative measurements of brain diffusion-weighted images (DWI) were performed at 3 time-points: Before the race, upon arrival and after 48 h. Multiple blood biomarkers were simultaneously investigated. Data analyses included brain apparent diffusion coefficient (ADC) and physiological data comparisons between three time-points. Results:The whole brain ADC significantly increased from baseline to arrival (p = 0.005) and then significantly decreased at recovery (p = 0.005) to lower values than at baseline (p = 0.005). While sodium, potassium, calcium, and chloride as well as hematocrit (HCT) changed over time, the serum osmolality remained constant. Significant correlations were found between whole brain ADC changes and osmolality (p = 0.01), cholesterol (p = 0.009), c-reactive protein (p = 0.04), sodium (p = 0.01), and chloride (p = 0.002) plasma level variations. Conclusions:These results suggest the relative increase of the inter-cellular volume upon arrival, and subsequently its reduction to lower values than at baseline, indicating that even after 48 h the brain has not fully recovered to its equilibrium state. Even though serum electrolytes may only indirectly indicate modifications at the brain level due to the blood brain barrier, the results concerning osmolality suggest that body water might directly influence the change in cerebral ADC. These findings establish therefore a direct link between general brain inter-cellular water content and physiological biomarkers modifications produced by extreme sport
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