Population Ecology and Reproductive Biology of The Diamondback Watersnake, Nerodia Rhombifer (Serpentes: Colubridae), in Southernmost Texas

Nerodia rhombifer is a polytypic, semi-aquatic snake with a broad geographical distribution ranging from the American Midwest southward to Chiapas, Mexico. Although relatively abundant throughout much of its range, few ecological studies of the species have been conducted. This study provides basic population ecology information in a subtropical habitat. Population data were obtained in a mark-recapture study at Santa Ana National Wildlife Refuge, Hidalgo County, Texas, from August 1995 to December 1998. Specimens taken elsewhere in Hidalgo County provided information on the reproductive biology. This study provides the first absolute density estimates from anywhere within the species’ range. Quantitative information on seasonal activity, growth rates, size dimorphism, population structure, reproductive cycles, and lipid dynamics are presented

Evaluación de los planes y programas para la erradicación del trabajo infantil. Caso de estudio: corporación de abastos de Bogotá S.A.

El trabajo infantil, entendido como una violación de los derechos humanos fundamentales, genera efectos sobre el desarrollo de los niños. Esto conlleva a generar daños físicos o psicológicos, con repercusiones durante toda su vida. En escenarios extremos, el trabajo infantil incorpora, entre otros hechos, la esclavitud de los niños, el rompimiento de núcleos familiares, la exposición a peligros y el padecimiento de enfermedades graves. Partiendo de esta realidad y tomando como base la normatividad nacional, aunado a los convenios que sobre el tema de trabajo infantil, ha proferido la Organización Internacional del Trabajo (OIT), como ente rector a nivel mundial de este tema; se llega a los planes y programas que a nivel distrital y en especial en la Corporación de Abastos de Bogotá S.A. – Corabastos, se han generado para erradicar este problema. El presente escrito permite identificar las causas que originan el surgimiento del trabajo infantil, los riesgos a que están expuestos los niños, niñas y adolescentes, así como las distintas formas de trabajo infantil existentes, las cuales en determinado momento pueden llegar a presentarse en la central mayorista más grande del país. De esta manera una vez verificados los planes y programas contra la erradicación del trabajo infantil que se plantean al interior de este sitio, se generan las conclusiones y se presentan algunas recomendaciones en pro de la erradicación y/o prevención de este flagelo que pareciera perdurar en el tiempo.Child labor is considered a violation of fundamental human rights and harms the development of children, which can lead to physical or psychological damage that can affect them even during their entire lives. In the worst cases, child labor can enslave children, separate them from their families, expose them to serious dangers and illnesses and / or leave them to their own devices on the streets of large cities, often when they are still very young. Starting from this reality and taking as a basis the national normativity, together with the agreements that on the issue of child labor, has been made by the International Labor Organization (ILO), as the world governing body on this issue; we reach the plans and programs that at the district level and especially in the Corporation of Supplies of Bogotá - Corabastos, have been generated to eradicate this problem. The present document identifies the causes that give rise to child labor, the risks to which children and adolescents are exposed, as well as the different forms of child labor that exist, which at a certain moment may occur in the central biggest wholesaler in the country. In this way, once the plans and programs against the eradication of child labor that are raised within the wholesale center are verified, the conclusions are generated and some recommendations are presented in favor of the eradication and / or prevention of this scourge that seems to last in the time

Depletion of the oncoprotein Bcl-3 induces centrosome amplification and aneuploidy in cancer cells

Bcl-3 is an atypical member of the inhibitor of NF-kappa B family of proteins since it can function as a coactivator of transcription. Although this oncogene was described in leukemia, it is overexpressed in a number of solid tumors as well. The oncogenic potential of Bcl-3 has been associated with its capacity to increase proliferation by means of activating the cyclin D1 promoter and to its antiapoptotic role mediated by the inhibiton of p53 activity. In the course of dissecting these properties, we found that depleting Bcl-3 protein using shRNAs induce a decrease of proliferation and clonogenic survival associated with the induction of multinucleation and increased ploidy. These effects were associated with a DNA damage response, a delay in G2/M checkpoint and the induction of centrosome amplificatio

Computational evidence for an early, amplified systemic inflammation program in polytrauma patients with severe extremity injuries

Extremity and soft tissue injuries contribute significantly to inflammation and adverse in-hospital outcomes for trauma survivors; accordingly, we examined the complex association between clinical outcomes inflammatory responses in this setting using in silico tools. Two stringently propensity-matched, moderately/severely injured (Injury Severity Score > 16) patient sub-cohorts of ~30 patients each were derived retrospectively from a cohort of 472 blunt trauma survivors and segregated based on their degree of extremity injury severity (above or below 3 on the Abbreviated Injury Scale). Serial blood samples were analyzed for 31 plasma inflammatory mediators. In addition to standard statistical analyses, Dynamic Network Analysis (DyNA) and Principal Component Analysis (PCA) were used to model systemic inflammation following trauma. Patients in the severe extremity injury sub-cohort experienced longer intensive care unit length of stay (LOS), total LOS, and days on a mechanical ventilator, with higher Marshall Multiple Organ Dysfunction (MOD) Scores over the first 7 days post-injury as compared to the mild/moderate extremity injury sub-cohort. The higher severity cohort had statistically significant elevated lactate, base deficit, and creatine phosphokinase on first blood draw, along with significant changes in multiple circulating inflammatory mediators. DyNA pointed to a sustained role for type 17 immunity in both sub-cohorts, along with IFN-γ in the severe extremity injury group. DyNA network complexity increased over 7 days post-injury in the severe injury group, while generally decreasing over this same time period in the mild/moderate injury group. PCA suggested a more robust activation of multiple pathways in the severe extremity injury group as compared to the mild/moderate injury group. These studies thus point to the possibility of self-sustaining inflammation following severe extremity injury vs. resolving inflammation following less severe extremity injury

Highly concentrated and stable few-layers graphene suspensions in pure and volatile organic solvents

Highly stable graphene suspensions in pure organic solvents, including volatile solvents such as ethanol, tetrahydrofurane, chloroform, acetone or toluene have been prepared by re-dispersion of a graphene-powder. Such re-dispersable solid is produced by precipitation or solvent elimination from graphene suspensions obtained by sonication of graphite in several organic solvent-water mixtures. Re-dispersion is feasible in a wide range of pure organic solvents, obtaining high quality few-layers graphene flakes stable in suspension for months. As a proof-of-concept, on-glass spray deposition of some of these suspensions, e.g. ethanol or tetrahydrofuran, results on electrically conductive transparent coatings. These results suggest industrial potential use of the scalable technology here developed to fabricate low-cost devices with many different potential applicationsThis research was financially supported by Abengoa Co., the Spanish Ministry of Economy and Competitiveness (MAT2013-46753-C2-1-P and RYC2012-09864) and Comunidad de Madrid (CAM 09-S2009_MAT-1467

Gender-based reciprocal expression of transforming growth factor-β1 and the inducible nitric oxide synthase in a rat model of cyclophosphamide-induced cystitis

<p>Abstract</p> <p>Background</p> <p>The pluripotent cytokine transforming growth factor-β1 (TGF-β1) is the central regulator of inducible Nitric Oxide Synthase (iNOS) that is responsible for nitric oxide (NO) production in inflammatory settings. Previous studies have implicated a role for NO, presumably derived from iNOS, in cyclophosphamide (CYP)-induced cystitis in the bladder. TGF-β1 is produced in latent form and requires dissociation from the latency-associated peptide (LAP) to act as primary anti-inflammatory and pro-healing modulator following tissue injury in the upper urinary tract. Since the role of TGF-β1 in lower urinary tract inflammation is currently unknown, and since gender-based differences exist in the setting of interstitial cystitis (IC), the present study examined the relationship between TGF-β1 and iNOS/NO in the pathogenesis of CYP-induced cystitis in both male and female rats.</p> <p>Methods</p> <p>Sprague-Dawley rats, 4 months of age, of either gender were given 150 mg/kg CYP intraperitoneally. Urinary and bladder tissue TGF-β1 and NO reaction products (NO₂^-/NO₃^-) were quantified as a function of time following CYP. Expression of active and latent TGF-β1 as well as iNOS in harvested bladder tissue was assessed by immunohistochemistry.</p> <p>Results</p> <p>Female rats had significantly higher levels of NO₂^-/NO₃^-in urine even at baseline as compared to male rats (p < 0.001), whereas there was no gender based significant difference in urine levels of active or latent TGF-β1 prior to CYP injection. Inflammatory and cytotoxic changes were induced by CYP in the bladder of both sexes that were accompanied by differences in the urine levels of NO₂^-/NO₃^-and TGF-β1. Male rats responded to CYP with significantly lower levels of NO₂^-/NO₃^-and significantly higher levels of TGF-β1 in urine (p < 0.05) as compared to females at all time points after CYP. The urine levels of NO₂^-/NO₃^-after CYP were inversely correlated to latent and active TGF-β1 (Pearson coefficient of -0.72 and -0.69 in females and -0.89 and -0.76 in males, respectively; p < 0.01). Bladder tissue of male rats exhibited significantly higher levels of both latent and active TGF-β1 (p < 0.01) compared to female rats after CYP. TGF-β1 and iNOS protein was mostly localized in the urothelium.</p> <p>Conclusion</p> <p>The results of this study suggest that there exists an inverse relationship between the expression of TGF-β1 and iNOS/NO₂^-/NO₃^-in CYP-inflamed bladder. The gender of the animal appears to magnify the differences in urine levels of TGF-β1 and NO₂^-/NO₃^-in this inflammatory setting. These results support the hypothesis that TGF-β1 can suppress iNOS expression associated with bladder inflammation and reduce systemic levels of NO₂^-/NO₃^-, and further suggest that this feature of TGF-β1 can be harnessed for therapy and diagnosis of interstitial cystitis.</p

Crystalline fibres of a covalent organic framework through bottom-up microfluidic synthesis

A microfluidic chip has been used to prepare fibres of a porous polymer with high structural order, setting a precedent for the generation of a wide variety of materials using this reagent mixing approach that provides unique materials not accessible easily through bulk processes. The reaction between 1,3,5-tris(4-aminophenyl)benzene and 1,3,5-benzenetricarbaldehyde in acetic acid under continuous microfluidic flow conditions leads to the formation of a highly crystalline and porous covalent organic framework (hereafter denoted as MF-COF-1), consisting of fibrillar micro-structures, which have mechanical stability that allows for direct drawing of objects on a surfaceFinancial support from Spanish Government (Projects MAT2013-46753-C2-1-P and CTQ2014-53486-R) and FEDER are acknowledged. A. A. and J. P. L. would like to thank the financial support from the Swiss National Science Foundation (SNSF) through the project no. 200021_16017

The development mechanisms of selective attention in child population

La atención selectiva es la función cognitiva que orienta la atención hacia los objetos o estímulos que resultan relevantes evitando la distracción frente a aquellos que son irrelevantes. En general la literatura indica que el desempeño de la atención selectiva mejora notablemente con la edad, sin embargo, las diferencias vinculadas a esta variable no se cumplen para todas las condiciones de cantidad de estímulos distractores ni para todos los grupos de edad. Asimismo, aun no se ha explorado la contribución del control inhibitorio y la velocidad de procesamiento a esta mejora progresiva. Por este motivo, y debido a la escasez de estudios en población infantil, se propuso analizar la contribución relativa de la velocidad de procesamiento y de la inhibición al desempeño en una tarea de atención selectiva y búsqueda visual en esta etapa evolutiva. Para ello, se administró una tarea de búsqueda de conjunciones y una tarea simple de velocidad de respuesta a 295 niños de 6 a 13 años de edad. Los resultados permitieron plantear dos conclusiones principales: por un lado, que la atención selectiva mejora consistentemente durante la niñez y, por otro lado, que no existe un mecanismo general y exclusivo capaz de explicar estas diferencias durante esta etapa vital. Así, tanto la velocidad de procesamiento como el control inhibitorio contribuyen al desarrollo de la atención selectiva y ambos mecanismos cognitivos actúan de manera complementaria más que excluyente.Selective attention allows directs attention to relevant objects or stimuli, while avoiding distraction from those that are irrelevant. The literature indicates that performance in CVS tasks improves markedly with age. However, age-related differences are not met neither for every number of distractor conditions nor for all age groups. In addition, the contribution of inhibitory control and processing speed to this progressive improvement has not yet been explored. For this reason, and due to the scarcity of studies using CVS paradigm to evaluate selective attention in children, the present study aimed to analyze the relative contribution of processing speed and inhibition to performance during this evolutionary period in a selective attention and CVS task. To do this, a CVS and a simple response speed task were administered to 295 children aged 6-13 years old. Results offered two conclusions: first, selective attention improves during childhood; second, there is no general and exclusive mechanism capable of explaining these differences during this period. Thus, both processing speed and inhibitory control contribute to development of selective attention, and they seem to be more complementary mechanisms than excluding ones.Fil: Introzzi, Isabel Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Psicología Básica, Aplicada y Tecnología. Universidad Nacional de Mar del Plata. Facultad de Psicología. Instituto de Psicología Básica, Aplicada y Tecnología; ArgentinaFil: Aydmune, Yésica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Psicología Básica, Aplicada y Tecnología. Universidad Nacional de Mar del Plata. Facultad de Psicología. Instituto de Psicología Básica, Aplicada y Tecnología; ArgentinaFil: Zamora, Eliana Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Psicología Básica, Aplicada y Tecnología. Universidad Nacional de Mar del Plata. Facultad de Psicología. Instituto de Psicología Básica, Aplicada y Tecnología; ArgentinaFil: Vernucci, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Psicología Básica, Aplicada y Tecnología. Universidad Nacional de Mar del Plata. Facultad de Psicología. Instituto de Psicología Básica, Aplicada y Tecnología; ArgentinaFil: Ledesma, Ruben Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Psicología Básica, Aplicada y Tecnología. Universidad Nacional de Mar del Plata. Facultad de Psicología. Instituto de Psicología Básica, Aplicada y Tecnología; Argentin

A Dynamic View of Trauma/Hemorrhage-Induced Inflammation in Mice: Principal Drivers and Networks

Background: Complex biological processes such as acute inflammation induced by trauma/hemorrhagic shock/ (T/HS) are dynamic and multi-dimensional. We utilized multiplexing cytokine analysis coupled with data-driven modeling to gain a systems perspective into T/HS. Methodology/Principal Findings: Mice were subjected to surgical cannulation trauma (ST) ± hemorrhagic shock (HS; 25 mmHg), and followed for 1, 2, 3, or 4 h in each case. Serum was assayed for 20 cytokines and NO2-/NO3-. These data were analyzed using four data-driven methods (Hierarchical Clustering Analysis [HCA], multivariate analysis [MA], Principal Component Analysis [PCA], and Dynamic Network Analysis [DyNA]). Using HCA, animals subjected to ST vs. ST + HS could be partially segregated based on inflammatory mediator profiles, despite a large overlap. Based on MA, interleukin [IL]-12p40/p70 (IL-12.total), monokine induced by interferon-γ (CXCL-9) [MIG], and IP-10 were the best discriminators between ST and ST/HS. PCA suggested that the inflammatory mediators found in the three main principal components in animals subjected to ST were IL-6, IL-10, and IL-13, while the three principal components in ST + HS included a large number of cytokines including IL-6, IL-10, keratinocyte-derived cytokine (CXCL-1) [KC], and tumor necrosis factor-α [TNF-α]. DyNA suggested that the circulating mediators produced in response to ST were characterized by a high degree of interconnection/complexity at all time points; the response to ST + HS consisted of different central nodes, and exhibited zero network density over the first 2 h with lesser connectivity vs. ST at all time points. DyNA also helped link the conclusions from MA and PCA, in that central nodes consisting of IP-10 and IL-12 were seen in ST, while MIG and IL-6 were central nodes in ST + HS. Conclusions/Significance: These studies help elucidate the dynamics of T/HS-induced inflammation, complementing other forms of dynamic mechanistic modeling. These methods should be applicable to the analysis of other complex biological processes

Cardiac Arrest Disrupts Caspase-1 and Patterns of Inflammatory Mediators Differently in Skin and Muscle Following Localized Tissue Injury in Rats: Insights from Data-Driven Modeling

Background: Trauma often co-occurs with cardiac arrest and hemorrhagic shock. Skin and muscle injuries often lead to significant inflammation in the affected tissue. The primary mechanism by which inflammation is initiated, sustained, and terminated is cytokine-mediated immune signaling, but this signaling can be altered by cardiac arrest. The complexity and context sensitivity of immune signaling in general has stymied a clear understanding of these signaling dynamics. Methodology/Principal findings: We hypothesized that advanced numerical and biological function analysis methods would help elucidate the inflammatory response to skin and muscle wounds in rats, both with and without concomitant shock. Based on multiplexed analysis of inflammatory mediators, we discerned a differential interleukin (IL)-1α and IL-18 signature in skin vs. muscle, which was suggestive of inflammasome activation in the skin. Immunoblotting revealed caspase-1 activation in skin but not muscle. Notably, IL-1α and IL-18, along with caspase-1, were greatly elevated in the skin following cardiac arrest, consistent with differential inflammasome activation. Conclusions/Significance: Tissue-specific activation of Caspase-1 and the NLRP3 inflammasome appear to be key factors in determining the type and severity of the inflammatory response to tissue injury, especially in the presence of severe shock, as suggested via data-driven modeling