DNA extraction and quantification from human saliva deposited on fruits with human bite

Background: Among important aspects of forensic science there stand recovery, preservation and analysis of stains originated by body fluid. DNA Isolated from evidence stains help to exclude an innocent suspect or to identify a perpetrator upon PCR-based typing. This study reports extraction and quantification of DNA from human saliva deposited on fruits. The research work was conducted at Department of Forensic Sciences, University of Health Sciences, Lahore and WTO laboratory of University of Veterinary and Animal Sciences, Lahore.Methodology: DNA from saliva deposited on bitten fruits. DNA from 55 samples were extracted by Chelax method, Quantifiler® DNA Quantification Kit was used for quantification of the extracted samples and amplification of DNA was done in 7500 Real-Time PCR systems (Applied Biosystems, Foster City, CA). Total 55 samples including controls (positive and negative controls) were collected from bitten fruits by sterile swabs.Results: All sample swabs with human saliva showed result for quantification. Overall good yield of DNA quantification obtained from all fruits and no sample showed internal inhibition. No sample showed non informative or incomplete quantification which occurs due to LCN (Low copy number) or lesser quantity of DNA extracted from saliva swab.Conclusion: This study has provided with optimized protocol to isolate DNA from saliva found in very minute quantity on organic surfaces like fruits. Adaptation of this method can play a vital role in establishing new trends in human forensics practiced in Pakistan. Moreover, for future work in human forensics, this study can provide important practical basis

PHYSICAL AND INDEX PROPERTIES OF LATE PRECAMBRIAN AND CAMBRIAN SALT RANGE ROCKS OF PUNJAB

Thirteen rock types including Sandstone, Siltstone, Dolomite, Shale, Gypsum and Rock Salt collected from five different formations of Salt Range area of Pakistan were tested to assess their physical and index properties. Specific gravity, dry and wet unit weights, moisture contents, porosity, water absorption and slake durability index were determined. Results indicate that most of the rocks have their specific gravity and unit weight values in the standard range. Higher durability indices and low water absorption values of sandstones and dolomite make them suitable for construction materials. While higher porosity of Baghanwala sandstone, lower and upper Khewra sandstone indicates their potential as hydrocarbon bearing reservoir and at the same time make them an ideal candidate for greenhouse gases sequestration in the subsurface.&nbsp

Effects of a high-dose 24-h infusion of tranexamic acid on death and thromboembolic events in patients with acute gastrointestinal bleeding (HALT-IT): an international randomised, double-blind, placebo-controlled trial

Background: Tranexamic acid reduces surgical bleeding and reduces death due to bleeding in patients with trauma. Meta-analyses of small trials show that tranexamic acid might decrease deaths from gastrointestinal bleeding. We aimed to assess the effects of tranexamic acid in patients with gastrointestinal bleeding. Methods: We did an international, multicentre, randomised, placebo-controlled trial in 164 hospitals in 15 countries. Patients were enrolled if the responsible clinician was uncertain whether to use tranexamic acid, were aged above the minimum age considered an adult in their country (either aged 16 years and older or aged 18 years and older), and had significant (defined as at risk of bleeding to death) upper or lower gastrointestinal bleeding. Patients were randomly assigned by selection of a numbered treatment pack from a box containing eight packs that were identical apart from the pack number. Patients received either a loading dose of 1 g tranexamic acid, which was added to 100 mL infusion bag of 0·9% sodium chloride and infused by slow intravenous injection over 10 min, followed by a maintenance dose of 3 g tranexamic acid added to 1 L of any isotonic intravenous solution and infused at 125 mg/h for 24 h, or placebo (sodium chloride 0·9%). Patients, caregivers, and those assessing outcomes were masked to allocation. The primary outcome was death due to bleeding within 5 days of randomisation; analysis excluded patients who received neither dose of the allocated treatment and those for whom outcome data on death were unavailable. This trial was registered with Current Controlled Trials, ISRCTN11225767, and ClinicalTrials.gov, NCT01658124. Findings: Between July 4, 2013, and June 21, 2019, we randomly allocated 12 009 patients to receive tranexamic acid (5994, 49·9%) or matching placebo (6015, 50·1%), of whom 11 952 (99·5%) received the first dose of the allocated treatment. Death due to bleeding within 5 days of randomisation occurred in 222 (4%) of 5956 patients in the tranexamic acid group and in 226 (4%) of 5981 patients in the placebo group (risk ratio [RR] 0·99, 95% CI 0·82–1·18). Arterial thromboembolic events (myocardial infarction or stroke) were similar in the tranexamic acid group and placebo group (42 [0·7%] of 5952 vs 46 [0·8%] of 5977; 0·92; 0·60 to 1·39). Venous thromboembolic events (deep vein thrombosis or pulmonary embolism) were higher in tranexamic acid group than in the placebo group (48 [0·8%] of 5952 vs 26 [0·4%] of 5977; RR 1·85; 95% CI 1·15 to 2·98). Interpretation: We found that tranexamic acid did not reduce death from gastrointestinal bleeding. On the basis of our results, tranexamic acid should not be used for the treatment of gastrointestinal bleeding outside the context of a randomised trial

Comparison of different DNA preparatory methods for development of a universal direct PCR-RFLP workflow for identification of meat origin in food products

Abstract The quality and quantity of the extracted DNA are two key aspects for a successful PCR (Polymerase Chain Reaction) amplification. Moreover, reduction in time and cost required for DNA extraction are also two considerable factors, in cases when large number of samples are to be analyzed within a limited time-frame and budget. Accordingly, the aim of this study was to compare and optimize performance of five different DNA extraction methods by boiling meat tissues from cattle, buffalo, sheep, goat, chicken, camel, horse and dog in PBS (Phosphate Buffer Saline), distilled water, alkaline lysis buffers 1, 2 or 3. The results indicated that the boiling of meat and its products in alkaline lysis buffers was a good method to extract crude DNA. The optimized crude DNA extraction protocol was coupled with PCR-RFLP (Restriction Fragment Length Polymorphism) analysis for meat species differentiation. This developed workflow was tested on fifty-three commercial beef and mutton samples, out of which three samples were found to be adulterated. In conclusion, the rapid crude DNA extraction protocol has led to the development of a direct PCR-RFLP workflow that is simple, time-saving and cost-effective for PCR-based identification of different meat species

Molecular characterisation of Leptospira strains in Pakistan

Introduction: Leptospirosis affects a wide range of mammals, humans, and even a few poikilothermic animal species. In Pakistan, serological studies of equine leptospirosis have reported a prevalence of over 40%, but no study has ever been conducted towards molecular detection of Leptospira in horses. Material and Methods: Blood samples from 128 horses were screened using ELISA and 41 positive samples were examined for the presence of leptospiral DNA using specific primers for 16S rRNA gene. Results: Out of 41 tested samples, 20 samples were found to be PCR-positive, revealing a fragment of 306 bp after gel electrophoresis. Sequencing and phylogenetic analysis of positive samples revealed circulation of pathogenic Leptospira spp. in Pakistani horses. No evidence of circulation of intermediate species was found in this study. Conclusion: This study reports the first molecular evidence of equine leptospirosis in Pakistan and lays ground for further research in this area. It also confirms the efficiency of 16S rRNA for the diagnosis of equine leptospirosis

A New Fractional Particle Swarm Optimization with Entropy Diversity Based Velocity for Reactive Power Planning

Optimal Reactive Power Dispatch (ORPD) is the vital concern of network operators in the planning and management of electrical systems to reduce the real and reactive losses of the transmission and distribution system in order to augment the overall efficiency of the electrical network. The principle objective of the ORPD problem is to explore the best setting of decision variables such as rating of the shunt capacitors, output voltage of the generators and tap setting of the transformers in order to diminish the line loss, and improve the voltage profile index (VPI) and operating cost minimization of standard electrical systems while keeping the variables within the allowable limits. This research study demonstrates a compelling transformative approach for resolving ORPD problems faced by the operators through exploiting the strength of the meta-heuristic optimization model based on a new fractional swarming strategy, namely fractional order (FO)–particle swarm optimization (PSO), with consideration of the entropy metric in the velocity update mechanism. To perceive ORPD for standard 30 and 57-bus networks, the complex nonlinear objective functions, including minimization of the system, VPI improvement and operating cost minimization, are constructed with emphasis on efficacy enhancement of the overall electrical system. Assessment of the results show that the proposed FO-PSO with entropy metric performs better than the other state of the art algorithms by means of improvement in VPI, operating cost and line loss minimization. The statistical outcomes in terms of quantile–quantile illustrations, probability plots, cumulative distribution function, box plots, histograms and minimum fitness evaluation in a set of autonomous trials validate the capability of the proposed optimization scheme and exhibit sufficiency and also vigor in resolving ORPD problems

Design of Fractional Particle Swarm Optimization Gravitational Search Algorithm for Optimal Reactive Power Dispatch Problems

In fact, optimal RPD is one of the most critical optimization matters related to electrical power stability and operation. The minimization of overall real power losses is obtained by adjusting the power systems control variables, for instance; generator voltage, compensated reactive power and tap changing of the transformer. In this search, a new heuristic computing method named as fractional particle swarm optimization gravitational search algorithm (FPSOGSA) is presented by introducing fractional derivative of velocity term in standard optimization mechanism. The designed FPSOGSA is implemented for the optimal RPD problems with IEEE-30 and IEEE-57 standards by attaining the near finest outcome sets of control variables along with minimization of two fitness objectives; active power transmission line losses ( $P_{loss,}$ MW) and voltage deviation ( $\text{V}_{\mathrm {D}}$ ). The superior performance of the proposed FPSOGSA is verified for both single and multiple runs through comparative study with state of art counterparts for each scenario of optimal RPD problems

Design of fractional evolutionary processing for reactive power planning with FACTS devices

Reactive power dispatch is a vital problem in the operation, planning and control of power system for obtaining a fixed economic load expedition. An optimal dispatch reduces the grid congestion through the minimization of the active power loss. This strategy involves adjusting the transformer tap settings, generator voltages and reactive power sources, such as flexible alternating current transmission systems (FACTS). The optimal dispatch improves the system security, voltage profile, power transfer capability and overall network efficiency. In the present work, a fractional evolutionary approach achieves the desired objectives of reactive power planning by incorporating FACTS devices. Two compensation arrangements are possible: the shunt type compensation, through Static Var compensator (SVC) and the series compensation through the Thyristor controlled series compensator (TCSC). The fractional order Darwinian Particle Swarm Optimization (FO-DPSO) is implemented on the standard IEEE 30, IEEE 57 and IEEE 118 bus test systems. The power flow analysis is used for determining the location of TCSC, while the voltage collapse proximity indication (VCPI) method identifies the location of the SVC. The superiority of the FO-DPSO is demonstrated by comparing the results with those obtained by other techniques in terms of measure of central tendency, variation indices and time complexity.info:eu-repo/semantics/publishedVersio

You can’t hide encoded evidence: DNA recovery from different fabrics after washing

<p>Bloodstains are extremely important forensic evidence for DNA profiling. The current study has been planned to quantify DNA recovery from a list of fabrics tested after washing. On each fabric, spots of blood was dotted, and the dried-bloodstained fabrics were hand washed with tap water, soaked in tap water with detergent and then hand washed, for 5 and 10 min. DNA was extracted through Chelex100 from these fabrics and quantified through Real Time PCR using the Quantifiler Human DNA Quantification kit. Results showed that DNA can be recovered from fabrics after they have been washed. The amount of recovered DNA is comparable among these fabrics and did not show significant variations with washing methods. Blends of fabrics showed the highest recovery while non-absorbent fabrics showed least DNA recovery. Knowledge of the different DNA quantities recovered from washed fabrics will be useful in solving criminal cases. The ability to recover DNA from washed fabrics could prove important in forensic casework.</p