Caracterización del proceso de exportación de flores exóticas – heliconias desde el departamento del Magdalena hacia el mercado japonés

La caracterización del proceso de exportación de flores exóticas al Japón, es el fruto de la recopilación de información secundaria que permite analizar si el departamento del Magdalena cumple con los requisitos necesarios para incursionar en el mercado oriental. En esta investigación se incluye un análisis del mercado nacional e internacional que hace referencia a las tendencias mundiales y al desarrollo que presenta Colombia en las principales ciudades exportadoras de heliconias. Además, se comparan los principales departamentos del país con el Magdalena para determinar factores positivos o negativos en el proceso de exportación de flores exóticas. Se evalúan los diferentes competidores, economía, entorno social, tendencias del mercado, Marketing mix; incluyendo el análisis de los datos con aspectos importantes a tener en cuenta en el momento de la exportación. Se muestran los procedimientos, requisitos y procesos que se deben seguir al momento de realizar la exportación de Heliconias al Japón

Tejiendo relaciones de amor

RESUMEN: El trabajo de grado “Representaciones del adulto mayor en el entorno familiar” es una investigación que busca conocer e identificar las representaciones sociales del adulto mayor a partir de las interacciones con la familia en el contexto de la vida cotidiana, por medio de aspectos que han vinculado la vida del adulto mayor a otros espacios de convivencia. Es posible entonces analizar la vida del adulto mayor y la familia, de acuerdo a las situaciones que forman parte de cada historia con relación a los cambios generados en el ciclo vital, las construcciones a partir de las experiencias generadas como producto de estos cambios en las relaciones de la familia con el adulto, lo cual ha desplegado cambios significativos en las concepciones frente a la etapa de la vejez. La intensión de indagar por las representaciones sociales de la población adulto mayor desde el contexto familiar permite centrar la mirada en aspectos que giran alrededor de la vida de cada abuelo y la familia como principal espacio de socialización por medio de las creencias, valores, costumbres y maneras de interactuar, lo cual se ha construido a lo largo de sus experiencias como producto de lo vivenciado a raíz de las decisiones, circunstancias y hechos que han modificado de alguna manera las dinámicas en el entorno familiar de cada uno de los abuelos

La imagen y la narrativa como herramientas para el abordaje psicosocial en escenarios de violencia en el Departamento de Sucre

La imagen y la narrativa como herramientas para el abordaje psicosocial en escenarios de violencia en el Departamento de Sucre.Resumen A partir del estudio y análisis reflexivo de los contenidos del curso, en este trabajo se presenta el abordaje realizado por el grupo, sobre la violencia del conflicto armado en Colombia, tomando como estrategia de acompañamiento la imagen y la narrativa. Se presenta el análisis del “Relato N° 2. Angélica”, escogido por el grupo, en el cual se analizan distintos fragmentos en los que se identifican situaciones vividas durante su desplazamiento de su sitio de origen, los traumas, tristeza, miedos y desolación, ocasionados por las distintas formas de violencia que enfrento junto a sus hijas. Basados en los interrogantes planteados, se profundiza desde la perspectiva psicosocial en aspectos relacionados con sus vivencias, mediante la narrativa, como una estrategia de intervención, pues según (White, 2016a, p. 29) cuando se realiza trabajo psicosocial con personas que han sufrido alguna alteración psicológica y emocional por la violencia, es importante estar atentos a lo que desean hablar con relación a su experiencia, a fin de que se convierta en una terapia, para la reconstrucción de sus vidas; se pueden ver en el relato, hechos relacionados con las dificultades y experiencias de miedo, dolor, rabia, la manera en que mediante recursos de afrontamiento, comienza a entender y enfrentar su realidad, se analizan los impactos psicosociales, el posicionamiento que asume como víctima y sobreviviente, la imágenes naturalizadas de la violencia, la emancipación discursiva, los significados alternos, de las imágenes dominantes de violencia y los emergentes psicosociales . Asumiendo el Rol de Psicólogo, en las experiencias relatadas en el caso, se realizan tres preguntas estratégicas, tres circulares y tres reflexivas, relacionadas con el relato escogido; entendiendo la pregunta como una forma de intervención, con la que las personas pueden hacer un examen a su situación y reflexionar de manera positiva, para la reconstrucción de sus vidas. De acuerdo a los interrogantes planteados, se analiza el Caso Cacarica y se diseñan tres estrategas de Acompañamiento Psicosocial, con base al enfoque Psicosocial, las técnicas narrativas, expresivas y performativas, el informe del paso 2, las conclusiones y Link página Wix.Abstract From the revision and reflexive analysis of the course contents, in this written will be posed the approach made by the study group and it is about the violence of the armed conflict in Colombia, taking the image and narrative as a strategy. The analysis of the tale number 2 “Relato No. 2 Angelica” was selected by the study group in which some different paragraphs are studied and are identified experiences lived by Angelica during her displacement from her home site, her traumas, sadness, fears and desolation caused by different ways of violence which she and her daughters had to face. Based in the question posed, it made deeper from the psychosocial perspective features relative with her experiences through the narrative as an intervention strategy according to (White, 2016b, p. 29) When a psychosocial job is made with people who has suffered any psychological modification because of the violence, is imperative to be attentive and listen to them when they want to express their experience and become a therapy as a purpose for construction of their lives. Also in the story it can detect facts associated with the difficulties and fear, painful, rage experiences, the way that they understand and face the reality through the coping resources. The psychosocial effects are analyzed, the situation such as victim and survivor she assumes, the naturalized images of violence, the discursive emancipation, the alternates meaning of the dominant images of violence and the emergent psychosocial. Taking the role of psychologist in accordance with all the experiences posed in the selected story it is formulated 3 strategic questions, 3 circular questions and 3 reflexive questions. Understanding the questions as a kind of intervention in which people can examine their situation and reflex in a positive way for recover their lives. Regarding to the interrogation, the group analyze the “Cacarica” case and create 3 strategies of psychosocial accompaniment based in the psychosocial focus, narrative, expressive and performative technics the report of the step 2, the conclusions and the link of the Wix page. Keywords: Conflict, coping, difficulties, painful, emerging, experiences, fear, psychosocial, trauma, violence

Enhancing Inhibition of Both α-Synuclein Aggregation and Neuroinflammation: New Insights into the C-9 Modification of Doxycycline

Doxycycline, a semi-synthetic tetracycline, is a widely used antibiotic for mild-to-moderate infections. However, its pleiotropic effects, such as anti-inflammatory and antioxidant properties, combined with its ability to interfere with α-synuclein inhibiting its aggregation, make it an attractive candidate for repositioning in Parkinson's disease treatment. Nevertheless, the antibiotic activity of doxycycline restricts its potential for long-term treatment of Parkinsonian patients. Eighteen novel doxycycline derivatives were designed with substitution at C9. Specifically, the dimethyl-amino group at C4 was reduced to significantly diminish the antibiotic activity, and several coupling reactions were performed at position C9 of the D ring. Using biophysical models, we found that seven compounds were more effective than the parent compound in inhibiting α-synuclein aggregation. Furthermore, two of these derivatives exhibited better anti-inflammatory effects at non-cytotoxic concentrations on microglial cell culture. Thus, we identified two design-based tetracyclines as the most promising candidates for further preclinical investigations. In addition, our study provides new insights into the structure-activity relationship of tetracyclines as neuroprotective molecules

A byproduct from the Valles Calchaquíes vineyards (Argentina) rich in phenolic compounds: A tool against endemic Leishmania dissemination

Vineyard-derived pomace is a byproduct of the wine industry that can have a negative impact on the environment if it is only disposed of or used as a fertilizer. Owing to its polyphenol content, grape pomace is an alternative to biocontrol undesirable microorganisms. In the present study, we characterized the phenolic composition of red and white grape pomace from Valles Calchaquíes, Argentina, and explored its activity against Leishmania (Leishmania) amazonensis, an etiological agent of American tegumentary leishmaniasis, a neglected endemic disease in northern Argentina. Red and white pomace extracts similarly reduced Leishmania viability after a 48-h treatment, with the fractions containing a higher proportion of phenolic compounds being more active. Both extracts stimulated ATPase activity on the parasite plasma membranes, with white grape pomace having a stronger effect than red grape pomace. In addition, the extracts displayed fairly good anticholinesterase activity, which may have contributed to their anti-Leishmania activity. These results reinforce the potential applicability of grape pomace as an antimicrobial agent for the development of biopesticides.Fil: Salazar, Paula Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Biológica; ArgentinaFil: Fanzone, Martín Leandro. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Mendoza-san Juan. Estacion Experimental Agropecuaria Mendoza. Agencia de Extension Rural Lujan de Cuyo.; ArgentinaFil: Zabala, Brenda Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Rodriguez Vaquero, Maria Jose. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cilli, Eduardo. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Barroso, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Minahk, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Biológica; ArgentinaFil: Acuña, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentin

Enhancing Inhibition of Both α-Synuclein Aggregation and Neuroinflammation: New Insights into the C-9 Modification of Doxycycline

Doxycycline, a semi-synthetic tetracycline, is a widely used antibiotic for mild-to-moderate infections. However, its pleiotropic effects, such as anti-inflammatory and antioxidant properties, combined with its ability to interfere with α-synuclein inhibiting its aggregation, make it an attractive candidate for repositioning in Parkinson\u27s disease treatment. Nevertheless, the antibiotic activity of doxycycline restricts its potential for long-term treatment of Parkinsonian patients. Eighteen novel doxycycline derivatives were designed with substitution at C9. Specifically, the dimethyl-amino group at C4 was reduced to significantly diminish the antibiotic activity, and several coupling reactions were performed at position C9 of the D ring. Using biophysical models, we found that seven compounds were more effective than the parent compound in inhibiting α-synuclein aggregation. Furthermore, two of these derivatives exhibited better anti-inflammatory effects at non-cytotoxic concentrations on microglial cell culture. Thus, we identified two design-based tetracyclines as the most promising candidates for further preclinical investigations. In addition, our study provides new insights into the structure-activity relationship of tetracyclines as neuroprotective molecules

Exploring the performance of Escherichia coli outer membrane vesicles as a tool for vaccine development against Chagas disease

BACKGROUND:Vaccine development is a laborious craftwork in which at least two main components must be defined: a highly immunogenic antigen and a suitable delivery method. Hence, the interplay of these elements could elicit the required immune response to cope with the targeted pathogen with a long-lasting protective capacity.OBJECTIVES:Here we evaluate the properties of Escherichia coli spherical proteoliposomes - known as outer membrane vesicles (OMVs) - as particles with natural adjuvant capacities and as antigen-carrier structures to assemble an innovative prophylactic vaccine for Chagas disease.METHODSTo achieve this, genetic manipulation was carried out on E. coli using an engineered plasmid containing the Tc24 Trypanosoma cruzi antigen. The goal was to induce the release of OMVs displaying the parasite protein on their surface.FINDINGS: As a proof of principle, we observed that native OMVs - as well as those carrying the T. cruzi antigen - were able to trigger a slight, but functional humoral response at low immunization doses. Of note, compared to the non-immunized group, native OMVs-vaccinated animals survived the lethal challenge and showed minor parasitemia values, suggesting a possible involvement of innate trained immunity mechanism.MAIN CONCLUSION: These results open the range for further research on the design of new carrier strategies focused on innate immunity activation as an additional immunization target and venture to seek for alternative forms in which OMVs could be used for optimizing vaccine development.Fil: Vázquez, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Mesias, Andrea Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Acuña, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Spangler, Joseph. Spece Sciences División. Naval Research Laboratory; Estados UnidosFil: Zabala, Brenda Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Parodi Ramoneda, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Thakur, Meghna. George Mason University; Estados UnidosFil: Oh, Eunkeu. Spece Sciences División. Naval Research Laboratory; Estados UnidosFil: Walper, Scott Allan. Spece Sciences División. Naval Research Laboratory; Estados UnidosFil: Pérez Brandan, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentin

Hoʻomoʻomoʻo: Piecing Together Expressions of Resistance

A handmade book of poetry representing a diversity of community women's perspectives on how militarization affects Hawaiʻi. It discusses the contradictions and differences among these women, due to different colonial histories that shape their relationship to militarization. But the book of poetry aims to weave their words together to bridge the divisions created by colonial and military oppression. Pieces include Artwork on Agent Orange by Asia Collier, I was born in a place/Filipino-Americans/They be watching us by Ellen-Rae Cachola, Hay(na)ku for Love in Occupied Nations by Grace Alvaro Caligtan, this is for the weavers by Melisa Casumbal, Michael of the Illest Rhymes by Nicki Sahagun Garces, Disneylands/How to Leave Your Mother by Brenda Kwon, Papatuanuku by Brandy McDougall, The Letter Speaks by Melanie Medalle, Map: Impacts of Militarization in Hawaiʻi/Nā Wahine Koa by Summer Nemeth, eJAMAICAtion by Jamaica Osorio, 4 years nad 1 anniversary are enough for me/The Meaning of Peace by Darlene Rodrigues, Between My Legs by Cawa Tran, A Kanaka Maoli Motherʻs Meditation and Prayer by Mahealani Wendt, On Militarism by Sonya Zabala, and a drawing of a woman kicking a tank by Gaby Ray

Protective Efficacy of the Epitope-Conjugated Antigen N-Tc52/TSkb20 in Mitigating <i>Trypanosoma cruzi</i> Infection through CD8+ T-Cells and IFNγ Responses

Chagas disease, caused by the protozoan Trypanosoma cruzi, remains a major public health challenge affecting millions in Latin America and worldwide. Although significant progress has been made in vector control, no vaccine exists to prevent infection or mitigate disease pathogenesis. We developed a rationally designed chimeric protein vaccine, N-Tc52/TSkb20, incorporating immunodominant epitopes from two T. cruzi antigens, the amino-terminal portion of Tc52 and the TSkb20 epitope derived from trans-sialidase. The objectives of this study were to construct and characterize the antigen and evaluate its protective potential in an immunoprophylactic murine model of T. cruzi infection. The N-Tc52/TSkb20 protein was recombinantly expressed in E. coli and its identity was confirmed using mass spectrometry and Western blotting. Immunization with the chimeric protein significantly controlled parasitemia and reduced the heart, colon, and skeletal muscle parasite burdens compared to non-vaccinated mice. Protection was superior to vaccination with the individual parental antigen components. Mechanistically, the vaccine induced potent CD8+ T-cell and IFNγ responses against the incorporated epitopes and a protective IgG antibody profile. A relatively low IL-10 response favored early parasite control. These results validate the promising multi-epitope approach and support the continued development of this type of rational vaccine design strategy against Chagas disease