A low-cost X-ray-transparent experimental cell for synchrotron-based X-ray microtomography studies under geological reservoir conditions

A new modular X-ray-transparent experimental cell enables tomographic investigations of fluid rock interaction under natural reservoir conditions (confining pressure up to 20 MPa, pore fluid pressure up to 15 MPa, temperature ranging from 296 to 473 K). The portable cell can be used at synchrotron radiation sources that deliver a minimum X-ray flux density of 109 photons mm−2s−1in the energy range 30–100 keV to acquire tomographic datasets in less than 60 s. It has been successfully used in three experiments at the bending-magnet beamline 2BM at the Advanced Photon Source. The cell can be easily machined and assembled from off-the-shelf components at relatively low costs, and its modular design allows it to be adapted to a wide range of experiments and lower-energy X-ray sources.</jats:p

A probabilistic damage model of stress-induced permeability anisotropy during cataclastic flow

Author Posting. © American Geophysical Union, 2007. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 112 (2007): B10207, doi:10.1029/2006JB004456.A fundamental understanding of the effect of stress on permeability evolution is important for many fault mechanics and reservoir engineering problems. Recent laboratory measurements demonstrate that in the cataclastic flow regime, the stress-induced anisotropic reduction of permeability in porous rocks can be separated into 3 different stages. In the elastic regime (stage I), permeability and porosity reduction are solely controlled by the effective mean stress, with negligible permeability anisotropy. Stage II starts at the onset of shear-enhanced compaction, when a critical yield stress is attained. In stage II, the deviatoric stress exerts primary control over permeability and porosity evolution. The increase in deviatoric stress results in drastic permeability and porosity reduction and considerable permeability anisotropy. The transition from stage II to stage III takes place progressively during the development of pervasive cataclastic flow. In stage III, permeability and porosity reduction becomes gradual again, and permeability anisotropy diminishes. Microstructural observations on deformed samples using laser confocal microscopy reveal that stress-induced microcracking and pore collapse are the primary forms of damage during cataclastic flow. A probabilistic damage model is formulated to characterize the effects of stress on permeability and its anisotropy. In our model, the effects of both effective mean stress and differential stress on permeability evolution are calculated. By introducing stress sensitivity coefficients, we propose a first-order description of the dependence of permeability evolution on different loading paths. Built upon the micromechanisms of deformation in porous rocks, this unified model provides new insight into the coupling of stress and permeability.W.Z. was partially supported by the National Science Foundation under grants NSF-OCE0221436 and NSF-EAR 0510459, and the Department of Energy under grant #DEFGO200ER15058 (WHOI). LM was supported by the National Science Foundation under grant NSF-EAR0337678

The recent history of the Galapagos Triple Junction preserved on the Pacific plate

Author Posting. © The Author(s), 2013. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Earth and Planetary Science Letters 371-372 (2013): 6-15, doi:10.1016/j.epsl.2013.04.018.At the Galapagos triple junction, the Cocos and Nazca plates are broken by a succession of transient rifts north and south of the Cocos-Nazca (C-N) Rift. Modeling has suggested that each rift initiated at the East Pacific Rise (EPR), its location controlled by the distance of the C-N Rift tip from the EPR. Evidence on the Pacific plate confirms that each transient rift formed a true RRR triple junction with the EPR and clarifies the history of the region. At ~1.5 Ma the triple junctions began jumping rapidly toward C-N Rift suggesting that the C-N Rift tip moved closer to the EPR. Pacific abyssal hills became broad and shallow indicating enhanced magma supply to the region. At ~1.4 Ma, the Galapagos microplate developed when extension became fixed on the southern transient rift to form the South scarp of the future Dietz rift basin. Lavas flooded the area and a Galapagos-Nazca magmatic spreading center initiated at the EPR. We suggest that a hotspot was approaching the southern triple junction from the west. The hotspot crossed to the Nazca plate ~1.25 Ma. Dietz seamount formed within the young spreading center, dikes intruded Dietz rift basin, and eruptions built volcanic ridges. Since ~0.8 Ma magmatic spreading has jumped northward twice, most recently to Dietz volcanic ridge. Amagmatic extension to the east has formed the large North scarp of Dietz rift basin. Northward jumping of the southern triple junction has maintained the microplate boundary close to the proposed hotspot.DKS was partially supported by NSF grant OCE-1028537, WZ by NSF grant EAR-1056317, and LM by NSF grant OCE-1060878

Experimental evidence for melt partitioning between olivine and orthopyroxene in partially molten harzburgite

Author Posting. © American Geophysical Union, 2016. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Solid Earth 121 (2016): 5776–5793, doi:10.1002/2016JB013122.Observations of dunite channels in ophiolites and uranium series disequilibria in mid-ocean ridge basalt suggest that melt transport in the upper mantle beneath mid-ocean ridges is strongly channelized. We present experimental evidence that spatial variations in mineralogy can also focus melt on the grain scale. This lithologic melt partitioning, which results from differences in the interfacial energies associated with olivine-melt and orthopyroxene-melt boundaries, may complement other melt focusing mechanisms in the upper mantle such as mechanical shear and pyroxene dissolution. We document here lithologic melt partitioning in olivine-/orthopyroxene-basaltic melt samples containing nominal olivine to orthopyroxene ratio of 3 to 2 and melt fractions of 0.02 to 0.20. Experimental samples were imaged using synchrotron-based X-ray microcomputed tomography at a resolution of 700 nm per voxel. By analyzing the local melt fraction distributions associated with olivine and orthopyroxene grains in each sample, we found that the melt partitioning coefficient, i.e., the ratio of melt fraction around olivine to that around orthopyroxene grains, varies between 1.1 and 1.6. The permeability and electrical conductivity of our digital samples were estimated using numerical models and compared to those of samples containing only olivine and basaltic melt. Our results suggest that lithologic melt partitioning and preferential localization of melt around olivine grains might play a role in melt focusing, potentially enhancing average melt ascent velocities.National Science Foundation Grant Numbers: 1250338, 1551300; Basic Energy Sciences Grant Number: DEFG0207ER15916; Advanced Photon Source Grant Number: DE-AC02-06CH113572017-02-2

Sliding of a rough surface under oblique loading

Author Posting. © American Geophysical Union, 2004. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 109 (2004): B05208, doi:10.1029/2004JB003027.Sliding of a rough surface having a range of asperity heights is a gradual process, starting at contacts under relatively low normal shear load and spreading until the surface slides as a unit. We analyze this process theoretically for asperities with spherical tips, with heights having a probability density distribution given by a negative exponential. The case where applied normal traction increases concurrently with applied shear is treated in detail, resulting in analytical expressions for the normal and shear displacements. These results are used to show limitations on constitutive behavior for more complex normal stress-shear stress histories.J. B. Walsh was supported by grant EAR- 9903217 from the National Science Foundation. W. Zhu gratefully acknowledges the support of the Department of Energy through grant DE-FG02-00ER15058 and WHOI Mellon Independent Study Award (2000)

A new model for analyzing the effect of fractures on triaxial deformation

Author Posting. © Elsevier B.V., 2006. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in International Journal of Rock Mechanics and Mining Sciences 43 (2006): 1241-1255, doi:10.1016/j.ijrmms.2006.04.002.Rock is porous, with a connected network of cracks and pores. The static and dynamic behaviors of a rock sample under load depend on both the solid mineral matrix and the porous phase. In general, the configuration of the pore phase is complex; thus most studies on the effect of the porous phase on rock deformation are conducted numerically and theoretical analyses of the constitutive relations are scarce. We have studied rock deformation under axially-symmetric loading by analyzing a model where the pore phase is approximated by rough planes, randomly spaced and oriented, extending through the sample. The roughness is caused by asperities, all with the same tip radii, but having heights h with a probability density distribution given by the negative exponential e-h/λ where λ is a length parameter. Slip at contacts under local shear stress is resisted by simple Coulomb friction, with friction coefficient f. Both static and dynamic deformation were analyzed. The effect of porosity on deformation for both modes was found to be given by the non-dimensional parameter λαj, where αj is the total area of the fault planes per unit volume. We demonstrate that stress-induced microfracturing begins as randomly oriented microslip throughout the sample. As axial load increases, microslip occurs along preferred orientations and locations, which finally leads to deformation on a single fault. The model was found to fault under static loading conditions---the axial load at faulting and the angle of the “fracture” plane agree with values of those parameters given by Coulomb’s theory of fracture. Dynamic moduli and Poisson’s ratio are found to be virtually elastic and independent of the friction coefficient acting at contacts. The attenuation for uniaxial dynamic loading is a strong function of the friction coefficient and increases linearly with strain amplitude, in agreement with laboratory measurements.W.Z. was partially supported by the Department of Energy under grant #DEFGO200ER15058 and the National Science Foundation under grant NSF-OCE0221436 (WHOI). Financial support for J.B.W. was provided by NSF grants EAR-9903217 and EAR- 0336840

IGF2BP2 Overexpression Indicates Poor Survival in Patients with Acute Myelocytic Leukemia

Background/Aims: IGF2BP2 has been reported to serve as an oncogene in various solid cancers. However, the role of IGF2BP2 in acute myelocytic leukemia (AML) is still unknown. Methods: Public databases Gene Omnibus was used to evaluate the expression of IGF2BP2 in AML patients and healthy controls. In addition, primary cells from these two populations were prepared by Ficoll density centrifugation. Rt-qPCR and western blot were used to detect IGF2BP2 expression in the primary cells from these two populations. Meta-analysis was performed to evaluate the association of IGF2BP2 and prognosis. Lentivirus-based shRNAs were used to knock down IGF2BP2 in AML cell lines KG-1a and Kasumi. Results: We searched the public database Gene Omnibus and analyzed IGF2BP2 expression in both AML and healthy populations. The results showed that IGF2BP2 was overexpressed in AML patients. To verify this phenomenon in fresh human samples, we compared the expression of IGF2BP2 in primary cells from 10 AML patients and 10 healthy controls and found that the expression of IGF2BP2 was upregulated in AML primary cells. More importantly, we observed that IGF2BP2 expression was negatively correlated with the CEBPA mutation status, which is an indicator of good prognosis (RR=0.648, p=0.0001). In addition, IGF2BP2 expression was positively associated with poor prognostic factors, such as the FLT3-ITD mutation (RR=1.198, p=0.0009) and IDH1 mutation (RR=1.354, p=0.0003), as well as intermediate and poor cytogenetic risk (RR=1.214, p=0.0026). To evaluate the prognostic value of IGF2BP2 in AML, we further performed a meta-analysis of 8 studies consisting of 1731 patients and found that IGF2BP2 overexpression was correlated with worse overall survival in AML patients [HR=1.31(1.16-1.49); p = 0.00]. Furthermore, we performed Gene Omnibus and Gene Set Enrichment analyses and found that the genes regulated by IGF2BP2 were mainly enriched in cell proliferation. IGF2BP2 knockdown by 4 different shRNA vectors significantly inhibited the growth of two AML cell lines, KG-1a and Kasumi. Conclusion: Thus, IGF2BP2 may serve as a biomarker to predict the prognosis of AML and as a potential target in AML

Regulation of serotonin production by specific microbes from piglet gut

Abstract Background Serotonin is an important signaling molecule that regulates secretory and sensory functions in the gut. Gut microbiota has been demonstrated to affect serotonin synthesis in rodent models. However, how gut microbes regulate intestinal serotonin production in piglets remains vague. To investigate the relationship between microbiota and serotonin specifically in the colon, microbial composition and serotonin concentration were analyzed in ileum-cannulated piglets subjected to antibiotic infusion from the ileum when comparing with saline infusion. Microbes that correlated positively with serotonin production were isolated from piglet colon and were further used to investigate the regulation mechanisms on serotonin production in IPEC-J2 and a putative enterochromaffin cell line RIN-14B cells. Results Antibiotic infusion increased quantities of Lactobacillus amylovorus (LA) that positively correlated with increased serotonin concentrations in the colon, while no effects observed for Limosilactobacillus reuteri (LR). To understand how microbes regulate serotonin, representative strains of LA, LR, and Streptococcus alactolyticus (SA, enriched in feces from prior observation) were selected for cell culture studies. Compared to the control group, LA, LR and SA supernatants significantly up-regulated tryptophan hydroxylase 1 (TPH1) expression and promoted serotonin production in IPEC-J2 cells, while in RIN-14B cells only LA exerted similar action. To investigate potential mechanisms mediated by microbe-derived molecules, microbial metabolites including lactate, acetate, glutamine, and γ-aminobutyric acid were selected for cell treatment based on computational and metabolite profiling in bacterial supernatant. Among these metabolites, acetate upregulated the expression of free fatty acid receptor 3 and TPH1 while downregulated indoleamine 2,3-dioxygenase 1. Similar effects were also recapitulated when treating the cells with AR420626, an agonist targeting free fatty acid receptor 3. Conclusions Overall, these results suggest that Lactobacillus amylovorus showed a positive correlation with serotonin production in the pig gut and exhibited a remarkable ability to regulate serotonin production in cell cultures. These findings provide evidence that microbial metabolites mediate the dialogue between microbes and host, which reveals a potential approach using microbial manipulation to regulate intestinal serotonin biosynthesis

Permeability-porosity relationships in seafloor vent deposits : dependence on pore evolution processes

Author Posting. © American Geophysical Union, 2007. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 112 (2007): B05208, doi:10.1029/2006JB004716.Systematic laboratory measurements of permeability and porosity were conducted on three large vent structures from the Mothra Hydrothermal vent field on the Endeavor segment of the Juan de Fuca Ridge. Geometric means of permeability values obtained from a probe permeameter are 5.9 × 10−15 m2 for Phang, a tall sulfide-dominated spire that was not actively venting when sampled; 1.4 × 10−14 m2 for Roane, a lower-temperature spire with dense macrofaunal communities growing on its sides that was venting diffuse fluid of <300°C; and 1.6 × 10−14 m2 for Finn, an active black smoker with a well-defined inner conduit that was venting 302°C fluids prior to recovery. Twenty-three cylindrical cores were then taken from these vent structures. Permeability and porosity of the drill cores were determined on the basis of Darcy's law and Boyle's law, respectively. Permeability values range from ∼10−15 to 10−13 m2 for core samples from Phang, from ∼10−15 to 10−12 m2 for cores from Roane, and from ∼10−15 to 3 × 10−13 m2 for cores from Finn, in good agreement with the probe permeability measurements. Permeability and porosity relationships are best described by two different power law relationships with exponents of ∼9 (group I) and ∼3 (group II). Microstructural analyses reveal that the difference in the two permeability-porosity relationships reflects different mineral precipitation processes as pore space evolves within different parts of the vent structures, either with angular sulfide grains depositing as aggregates that block fluid paths very efficiently (group I), or by late stage amorphous silica that coats existing grains and reduces fluid paths more gradually (group II). The results suggest that quantification of permeability and porosity relationships leads to a better understanding of pore evolution processes. Correctly identifying permeability and porosity relationships is an important first step toward accurately estimating fluid distribution, flow rate, and environmental conditions within seafloor vent deposits, which has important consequences for chimney growth and biological communities that reside within and on vent structures.Support from the National Science Foundation under grants NSF OCE-9986456 (W.Z. and M.K.T.) and NSF OCE-0327488 (P.R.C.) is gratefully acknowledged. We also thank the WHOI summer student fellowship for providing support to H.G