Antioxidant phytocomplexes extracted from pomegranate (Punica granatum L.) using hydrodynamic cavitation show potential anticancer activity in vitro

Hydrodynamic cavitation (HC), as an effective, efficient, and scalable extraction technique for natural products, could enable the affordable production of valuable antioxidant extracts from plant resources. For the first time, whole pomegranate (Punica granatum L.) fruits, rich in bioactive phytochemicals endowed with anti-cancer properties, were extracted in water using HC. Aqueous fractions sequentially collected during the process (M1–M5) were lyophilized (L), filtered (A), or used as such, i.e., crude (C), and analyzed for their biochemical profile and in vitro antioxidant power. The fractions M3 and M4 from the L and C series showed the highest antiradical activity and phytochemical content. While the lyophilized form is preferable for application purposes, sample L-M3, which was produced faster and with lower energy consumption than M4, was used to assess the potential antiproliferative effect on human breast cancer line (AU565-PAR) and peripheral blood mononuclear (PBMC) cells from healthy donors. In a pilot study, cell growth, death, and redox state were assessed, showing that L-M3 significantly reduced tumor cell proliferation and intracellular oxygen reactive species. No effect on PBMCs was detected. Thus, the antioxidant phytocomplex extracted from pomegranate quickly (15 min), at room temperature (30 °C), and efficiently showed potential anticancer activity without harming healthy cells

Volatile Compounds of Lemon and Grapefruit IntegroPectin

An HS-SPME GC-MS analysis of the volatile compounds adsorbed at the outer surface of lemon and grapefruit pectins obtained via the hydrodynamic cavitation of industrial waste streams of lemon and grapefruit peels in water suggests important new findings en route to understanding the powerful and broad biological activity of these new pectic materials. In agreement with the ultralow degree of esterification of these pectins, the high amount of highly bioactive α-terpineol and terpinen-4-ol points to limonene (and linalool) decomposition catalyzed by residual citric acid in the citrus waste peel residue of the juice industrial production

Double-Stranded RNA Attenuates the Barrier Function of Human Pulmonary Artery Endothelial Cells

Circulating RNA may result from excessive cell damage or acute viral infection and can interact with vascular endothelial cells. Despite the obvious clinical implications associated with the presence of circulating RNA, its pathological effects on endothelial cells and the governing molecular mechanisms are still not fully elucidated. We analyzed the effects of double stranded RNA on primary human pulmonary artery endothelial cells (hPAECs). The effect of natural and synthetic double-stranded RNA (dsRNA) on hPAECs was investigated using trans-endothelial electric resistance, molecule trafficking, calcium (Ca2+) homeostasis, gene expression and proliferation studies. Furthermore, the morphology and mechanical changes of the cells caused by synthetic dsRNA was followed by in-situ atomic force microscopy, by vascular-endothelial cadherin and F-actin staining. Our results indicated that exposure of hPAECs to synthetic dsRNA led to functional deficits. This was reflected by morphological and mechanical changes and an increase in the permeability of the endothelial monolayer. hPAECs treated with synthetic dsRNA accumulated in the G1 phase of the cell cycle. Additionally, the proliferation rate of the cells in the presence of synthetic dsRNA was significantly decreased. Furthermore, we found that natural and synthetic dsRNA modulated Ca2+ signaling in hPAECs by inhibiting the sarco-endoplasmic Ca2+-ATPase (SERCA) which is involved in the regulation of the intracellular Ca2+ homeostasis and thus cell growth. Even upon synthetic dsRNA stimulation silencing of SERCA3 preserved the endothelial monolayer integrity. Our data identify novel mechanisms by which dsRNA can disrupt endothelial barrier function and these may be relevant in inflammatory processes

A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

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Early Devonian (Late Emsian) shark fin remains (Chondrichthyes) from the Paraná Basin, southern Brazil

This is an open-access article distributed under the terms of the Creative Commons Attribution License [Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)]. The attached file is the published version of the article

Genesis and Distribution of Low Fluvial Terraces Formed by Holocene Climate Pulses in Brazil

Low fluvial terraces present azonal spatialization, encompassing several geomorphological compartments and climate zones in Brazil. Their genesis is directly related to river dynamics. When influenced by allogenic forces, such as Holocene climate pulses, it results in channel incision and posterior abandonment of the floodplain. Relatively plain landforms at different altimetric levels identified between the current floodplain and hillslope (low river terraces) are a result of these processes. Previous works using Optically Stimulated Luminescence (OSL) in low terraces of several rivers in Brazil have indicated morpho-chronologic similarities between depositional events, raising the hypothesis of feedbacks and fluvial adjustments relatively simultaneous to Holocene climate events. Considering these dynamics, this study employed OSL to obtain absolute dating information for 114 samples taken from distinct levels of the low river terraces of 30 rivers in Brazil, integrating the database of the IG-UNICAMP laboratory of Geomorphology and Environmental Analysis. Based on the data and statistical analysis (cluster and correlation analysis), this study aimed to identify relationships between different variables which might have controlled spatial homogenous and heterogeneous feedbacks during distinct paleoenvironmental contexts. The proposed methodology tested a fundamental hypothesis of the regional climatic geomorphology, and the results obtained may contribute to future discussions on the relationship between low river terraces and anthropic occupation

Genesis and Distribution of Low Fluvial Terraces Formed by Holocene Climate Pulses in Brazil

Low fluvial terraces present azonal spatialization, encompassing several geomorphological compartments and climate zones in Brazil. Their genesis is directly related to river dynamics. When influenced by allogenic forces, such as Holocene climate pulses, it results in channel incision and posterior abandonment of the floodplain. Relatively plain landforms at different altimetric levels identified between the current floodplain and hillslope (low river terraces) are a result of these processes. Previous works using Optically Stimulated Luminescence (OSL) in low terraces of several rivers in Brazil have indicated morpho-chronologic similarities between depositional events, raising the hypothesis of feedbacks and fluvial adjustments relatively simultaneous to Holocene climate events. Considering these dynamics, this study employed OSL to obtain absolute dating information for 114 samples taken from distinct levels of the low river terraces of 30 rivers in Brazil, integrating the database of the IG-UNICAMP laboratory of Geomorphology and Environmental Analysis. Based on the data and statistical analysis (cluster and correlation analysis), this study aimed to identify relationships between different variables which might have controlled spatial homogenous and heterogeneous feedbacks during distinct paleoenvironmental contexts. The proposed methodology tested a fundamental hypothesis of the regional climatic geomorphology, and the results obtained may contribute to future discussions on the relationship between low river terraces and anthropic occupation