Kir5.1 regulates Nedd4-2-mediated ubiquitination of Kir4.1 in distal nephron.

Kir4.1/5.1 heterotetramer participates in generating the negative cell membrane potential in distal convoluted tubule (DCT) and plays a critical role in determining the activity of Na-Cl cotransporter (NCC). Kir5.1 contains a phosphothreonine motif at its COOH terminus (AA249-252). Coimmunoprecipitation showed that Nedd4-2 was associated with Kir5.1 in HEK293 cells cotransfected with Kir5.1 or Kir4.1/Kir5.1. GST pull-down further confirmed the association between Nedd4-2 and Kir5.1. Ubiquitination assay showed that Nedd4-2 increased the ubiquitination of Kir4.1/Kir5.1 heterotetramer in the cells cotransfected with Kir4.1/Kir5.1, but it has no effect on Kir4.1 or Kir5.1 alone. Patch-clamp and Western blot also demonstrated that coexpression of Nedd4-2 but not Nedd4-1 decreased K currents and Kir4.1 expression in the cells cotransfected with Kir4.1 and Kir5.1. In contrast, Nedd4-2 fails to inhibit Kir4.1 in the absence of Kir5.1 or in the cells transfected with the inactivated form of Nedd4-2 (Nedd4-2C821A). Moreover, the mutation of TPVT motif in the COOH terminus of Kir5.1 largely abolished the association of Nedd4-2 with Kir5.1 and abolished the inhibitory effect of Nedd4-2 on K currents in HEK293 cells transfected with Kir4.1 and Kir5.1 mutant (Kir5.1T249A). Finally, the basolateral K conductance in the DCT and Kir4.1 expression is significantly increased in the kidney-specific Nedd4-2 knockout or in Kir5.1 knockout mice in comparison to their corresponding wild-type littermates. We conclude that Nedd4-2 binds to Kir5.1 at the phosphothreonine motif of the COOH terminus, and the association of Nedd4-2 with Kir5.1 facilitates the ubiquitination of Kir4.1, thereby regulating its plasma expression in the DCT

Aerosol particles at a high-altitude site on the Southeast Tibetan Plateau, China: Implications for pollution transport from South Asia

      Bulk aerosol samples were collected from 16 July 2008 to 26 July 2009 at Lulang, a high-altitude (>3300m above sea level) site on the southeast Tibetan Plateau (TP); objectives were to determine chemical characteristics of the aerosol and identify its major sources. We report aerosol (total suspended particulate, TSP) mass levels and the concentrations of selected elements, carbonaceous species, and water-soluble inorganic ions. Significant buildup of aerosol mass and chemical species (organic carbon, element carbon, nitrate, and sulfate) occurred during the premonsoon, while lower concentrations were observed during the monsoon. Seasonal variations in aerosol and chemical species were driven by precipitation scavenging and atmospheric circulation. Two kinds of high-aerosol episodes were observed: one was enriched with dust indicators (Fe and Ca2+), and the other was enhanced with organic and elemental carbon (OC and EC), SO42−, NO3−, and Fe. The TSP loadings during the latter were 3 to 6 times those on normal days. The greatest aerosol optical depths (National Centers for Environmental Protection/National Center for Atmospheric Research reanalysis) occurred upwind, in eastern India and Bangladesh, and trajectory analysis indicates that air pollutants were transported from the southwest. Northwesterly winds brought high levels of natural emissions (Fe, Ca2+) and low levels of pollutants (SO42−, NO3−, K+, and EC); this was consistent with high aerosol optical depths over the western deserts and Gobi. Our work provides evidence that both geological and pollution aerosols from surrounding regions impact the aerosol population of the TP

Non-contrast MR imaging of blood-brain barrier permeability to water

Neuro Imaging Researc

Strong deep-blue photoluminescence of mesographite boron nitride

10.1088/0953-8984/16/12/026Journal of Physics Condensed Matter16122181-2186JCOM

Investigation of individual CuO nanorods by polarized micro-Raman scattering

10.1016/j.jcrysgro.2004.04.097Journal of Crystal Growth2683-4 SPEC. ISS.590-595JCRG

The 600–580 Ma continental rift basalts in North Qilian Shan, northwest China: Links between the Qilian-Qaidam block and SE Australia, and the reconstruction of East Gondwana.

We report a sequence of thick, well-preserved basaltic lavas interlayered with shallow marine dolomitic carbonates, mudstones and siltstones of the Zhulongguan Group, in the western segment of the North Qilian orogen, northwest China. Two new zircon SIMS ages show that this sequence formed at ∼600–580 Ma. The mafic volcanics can be subdivided into tholeiitic and alkaline basalts, and have compositions similar to present-day ocean island basalt (OIB) or continental flood basalts. The occurrence, geochemical features and age data suggest that the Zhulongguan basalts originated at a continental rift setting in the latest Neoproterozoic, within the north margin of the Qilian-Qaidam block. This volcanic-sedimentary formation exhibits close affinity to the passive continental margin in southeastern Australia. Our observations favor a link of the Qilian-Qaidam block with SE Australia (also south China) during the breakup of Rodinia, thereby filling a void in existing reconstructions of the region

Effects of degree of three-dimensional order and Fe impurities on photoluminescence of boron nitride

10.1063/1.1772880Journal of Applied Physics9641947-1952JAPI