78 research outputs found

    Time course of hematological parameters in bleeding-induced anemia

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    In order to investigate daily changes of hematological parameters in bleeding-induced anemia, we treated Wistar albino male rats by daily bleeding (1.5-2 mL of blood from the tail vein for eight days). Blood samples were taken before (on day zero) and on the first to eighth days of bleeding. The values of hematocrit, hemoglobin, and erythrocyte count decreased significantly after the second, sixth, and second days of bleeding, respectively. The number of leukoĀ­cytes and platelets, as well as Heinz body levels, increased significantly after the third and second days of treatment. The percentage of reticulocytes increased significantly from the second day and attained the maximum level (32.55 Ā± 0.96%) on the eighth day

    Concentration of antioxidant compounds and lipid peroxidation in the liver and white muscle of hake (Merluccius merluccius L.) in the Adriatic sea

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    Specimens of a marine freshwater fish - hake (Merluccius merluccius L.) - were collected at the localities of Platamuni and Valdanos (Montenegro, South Adriatic) in the spring of 2003. Our results showed higher concentrations of LPO, Vit C, and Vit E in the liver in comparison with white muscle. The concentration of LPOin both tissues was higher, while that of Vit E was lower at Valdanos compared to Platamuni. These differences in parameters of oxidative stress are partly due to differences in temperature and the concentrations of nitrites, nitrates, and detergents in the waters of Valdanos compared to Platamuni.Tokom 2003. godine uzorkovane su jedinke morske ribe - oslića (Merluccius merluccius L.) iz voda sa lokaliteta Platamuni i Valdanos (Crna Gora, južni Jadran) u prolećnom periodu. Dobijeni rezultati su pokazali veće koncentracije LP, Vit Ci Vit E u jetri oslića u odnosu na belo miÅ”ićno tkivo. Koncentracija LP je bila veća, a Vit E manja u oba ispitivana tkiva oslića u vodama lokaliteta Valdanos u poređenju sa Platamuni. Ovakve promene parametara oksidacionog stresa posledica su promena temperature, kao i povećanih koncentracija nitrita, nitrata i deterdženata u vodama lokaliteta Valdanos u odnosu na Platamune.Projekat ministarstva br. 143035

    Effects of cisplatin on lipid peroxidation and the glutathione redox status in the liver of male rats: The protective role of selenium

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    The role of oxidative stress in cisplatin (CP) toxicity and its prevention by pretreatment with selenium (Se) was investigated. Male Wistar albino rats were injected with a single dose of cisplatin (7.5 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.) alone or in combination. The results suggest that CP intoxication induces oxidative stress and alters the glutathione redox status: reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio (GSH RI), resulting in increased lipid peroxidation (LPO) in rat liver. The pretreatment with selenium prior to CP treatment showed a protective effect against the toxic influence of CP on peroxidation of the membrane lipids and an altering of the glutathione redox status in the liver of rats. From our results we conclude that selenium functions as a potent antioxidant and suggest that it can control CP-induced hepatotoxicity in rats

    Effects of cisplatin on lipid peroxidation and the glutathione redox status in the liver of male rats: The protective role of selenium

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    The role of oxidative stress in cisplatin (CP) toxicity and its prevention by pretreatment with selenium (Se) was investigated. Male Wistar albino rats were injected with a single dose of cisplatin (7.5 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.) alone or in combination. The results suggest that CP intoxication induces oxidative stress and alters the glutathione redox status: reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio (GSH RI), resulting in increased lipid peroxidation (LPO) in rat liver. The pretreatment with selenium prior to CP treatment showed a protective effect against the toxic influence of CP on peroxidation of the membrane lipids and an altering of the glutathione redox status in the liver of rats. From our results we conclude that selenium functions as a potent antioxidant and suggest that it can control CP-induced hepatotoxicity in rats.U naÅ”oj studiji ispitivana je uloga oksidacionog stresa u toksičnosti cisplatina (SR) i njegova prevencija pretretmanom selenom (Se). Mužjaci Wistar albino pacova su inicirani jednom dozom cisplatina (7.5 mg CP/kg t.m., i.p.) i selena (6 mg Se/kg t.m, kao Na2SeO3, i.p.) pojedinačno ili u kombinaciji. Rezultati pokazuju da intoksikacija SR uzrokuje oksidacioni stres i promenu glutation redoks statusa: redukovanog (GSH), oksidovanog (GSSG) i GSH/GSSG indeksa (GSH RI), kao i povećanje lipidne peroksidacije (LPO) u jetri pacova. Tretman Se koji je prethodio tretmanu SR pokazao je zaÅ”titne efekte protiv toksičnog delovanja SR na peroksidaciju lipida membrane i promenu glutation redoks statusa u jetri pacova. Na osnovu naÅ”ih rezultata zaključujemo da Se, deluje kao snažan antioksidans i da može imati ulogu u kontroli SR indukovane hepatotoksičnosti kod pacova.Projekat ministarstva br. 143035

    Spatial variations in the distribution of trace ionic impurities in the water-steam cycle in a thermal power plant based on a multivariate statistical approach

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    In this study, a multivariate statistical approach was used to identify the key variables responsible for process water quality in a power plant. The ion species that could cause corrosion in one of the major thermal power plants (TPP) in Serbia were monitored. A suppressed ion chromatographic (IC) method for the determination of the target anions and cations at trace levels was applied. In addition, some metals important for corrosion, i.e., copper and iron, were also analysed by the graphite furnace atomic absorption spectrophotometric (GFAAS) method. The control parameters, i.e., pH, dissolved oxygen and silica, were measured on-line. The analysis of a series of representative samples from the TPP Nikola Tesla, collected in different plant operation modes, was performed. Every day laboratory and on-line analysis provides a large number of data in relation to the quality of water in the water-steam cycle (WSC) which should be evaluated and processed. The goal of this investigation was to apply multivariate statistical techniques and choose the most applicable technique for this case. Factor analysis (FA), especially principal component analysis (PCA) and cluster analysis (CA) were investigated. These methods were applied for the evaluation of the spatial/temporal variations of process water and for the estimation of 13 quality parameters which were monitored at 11 locations in the WSC in different working conditions during a twelve month period. It was concluded that PCA was the most useful method for identifying functional relations between the elements. After data reduction, four main factors controlling the variability were identified. Hierarchical cluster analysis (HCA) was applied for sample differentiation according to the sample location and working mode of the TPP. On the basis of this research, the new design of an optimal monitoring strategy for future analysis was proposed with a reduced number of measured parameters and with reduced frequency of their measurements
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