Phytoplancton du lac municipal de Yaoundé (Cameroun) : Succession écologique et structure des peuplements

L'évolution spatio-temporelle, qualitative et quantitative du peuplement phytoplanctonique a été suivie au lac municipal de Yaoundé. Pour cela des prélèvements hebdomadaires ont été effectués de novembre 1996 à décembre 1997, au niveau d'une station de la zone centrale du lac.Trois descripteurs (indice de diversité spécifique, régularité et diagrammes rangs-fréquences) ont été utilisés pour l'analyse de la dynamique de la structure du peuplement. L'indice de diversité spécifique obtenu à partir des biomasses spécifiques, par la formule dérivée de celle de SHANNON et WEAVER (1949), est compris entre 0,68 et 4,64 bits/µg. La régularité (PIELOU, 1966) varie de 0,14 à 0,84; les faibles valeurs correspondant dans l'ensemble à la présence des espèces fortement dominantes. Les profils des diagrammes rangs-fréquences, établis en coordonnées log-log, associés aux faibles valeurs de la diversité, sont essentiellement caractéristiques des stades pionniers (1 et 1') de l'évolution d'un lac tel que décrits par MARGALEF (1967) et FRONTIER (1976). Le stade 2 est rare alors que le stade 3 est absent.Une analyse simultanée basée sur les variations spatio-temporelles de la densité cellulaire et de l'indice de diversité spécifique, sur les valeurs de la régularité, et sur les profils des diagrammes rangs-fréquences révèle le caractère immature permanent des populations phytoplanctoniques inféodées à ce biotope, et caractérise ainsi un milieu eutrophe à hypereutrophe où aucun stade d'équilibre n'est atteint. La richesse du milieu en éléments biogènes et sa faible profondeur (Zmax=4,3 m) sont à l'origine de sa vulnérabilité. L'événement susceptible d'interrompre la succession des populations s'est avéré être le brassage des eaux, provoqué aussi bien par les pluies que les vents dont l'énergie cinétique est fréquemment suffisante pour entraîner un brassage complet d'une colonne d'eau de hauteur aussi faible.The Yaounde municipal lake (3°52'N, 11°32'E) is a shallow hypertrophic water body (Z max=4.3 m), situated downstream from Yaounde. It is an artificial lake resulting from a dam constructed in 1951 across a small river named Mingoa. Like many other water bodies in urbanised areas, this ecosystem switched rapidly from being mesotrophic to hypertrophic during the 1980's, due to the expansion of human activity and an inefficient treatment of wastewater in its catchment area. This study was carried out with the objective to analyse the ecological succession and the spatio-temporal fluctuations of the phytoplankton population structure in this shallow equatorial lake. Samples were collected at weekly intervals from November 1996 to December 1997, at a station near its central zone (depth=3.5 m), using an opaque horizontal PVC Van Dorn sampler. Sampling operations were conducted at the following levels: surface; -0.5 m; -1 m; -1.5 m and -2.5 m.Individual phytoplankton from 1% iodine acid preserved subsamples (BOURRELLY, 1990) were enumerated with an inverted microscope (Olympus CK2) at 200X magnification, using a Sedgwick-Rafter counting cell, according to UTHERMÖHL (1958). At least 400 individuals (colonies, bundles or trichomes, depending on existing life forms) were counted on each subsample. Specific biomass was then obtained using the biovolume method. To evaluate the population structure dynamics, the SHANNON and WEAVER (1949) specific diversity index method and the evenness (PIELOU, 1966) method were computerized using specific biomass, the evenness being the ratio between the real and the maximal diversity (log2 S, where S is the number of species). Also, rank-frequency diagrams were carried out in logarithmic co-ordinates according to FRONTIER (1969), using integrated samples obtained from grab samples collected over the entire water column.Cell densities were highest in the uppermost meter of the water column at the beginning of the rainy season, due to the exponential development of species such as Planktothrix mougeotii, Chlamydomonas sp., Eudorina elegans, Euglena gracilis, Gonyostomum semen, Euglena texta, Phacus helicoïdes, Closterium limneticum, Cryptomonas ovata, Peridinium cf. gutwinskii, Trachelomonas hispida var. crenulatocollis and Cyclotella meneghiniana. The specific diversity index varied 0.68 and 4.64 bits/µg, and values gradually decreased from the first uppermost meter to the bottom of the water column. Evenness varied from 0.14 to 0.84 with a somewhat similar spatio-temporal variation as the diversity index. Low values of these descriptors generally correspond to the presence of a highly dominant species. The rank-frequency diagrams were mainly S-shaped, indicating the predominance of one species which in this study was either Chlamydomonas sp or Planktothrix mougeotii.A simultaneous analysis of the ecological succession and the spatio-temporal variations of the phytoplanktonic structure revealed that the functioning of this ecosystem was essentially allogenic in nature and was strongly influenced by the supply of decayed organic matter from the Mingoa stream, and effluents from ministerial buildings and hotels located near the lake. This permanent supply of abundant biogenic elements, coupled with the low depth of the ecosystem, makes it vulnerable. Ecological succession, frequently limited to the pioneer stages (1 and 1'), reflected the briefness of the different phases of phytoplankton growth, and the consequences of disturbances regularly undergone by the phytoplankton community. The second stage was scarcely reached while the third was absent. These successions were mainly controlled by rainfall, as well as wind, which is responsible for the mixing of such a shallow water column. These results can be considered important for the Yaounde municipal lake and other water bodies close to densely populated areas in Cameroon for which there is no management plan. Urgent actions need to be carried out to rehabilitate this ecosystem that fluctuates between the eutrophic and hypertrophic status

Variations spatiales et temporelles de la richesse et de l'abondance des rotifères (Brachionidae et Trichocercidae) et des cladocères dans un petit lac artificiel eutrophe situé en zone tropicale

Les communautés de rotifères (Brachionidae et Trichocercidae) et de cladocères peuplant les eaux du lac Municipal de Yaoundé situé en zone tropicale (Cameroun), ont été examinés en relation avec quelques variables physico-chimiques du milieu. Des 26 espèces et sous-espèces de rotifères (Brachionidae et Trichocercidae) et 15 espèces de cladocères inventoriées, environ 16 sont identifiées pour la première fois au Cameroun. Parmi les espèces quantitativement dominantes, les espèces fréquemment rencontrées sont Brachionus angularis angularis, B. falcatus falcatus, et B. calyciflorus chez les Brachionidae, Trichocerca elongata elongata, et T. bicristata bicristata chez les Trichocercidae, et Ceriodaphnia cornuta et Chydorus eurynotus chez les cladocères. Plus de 75% des espèces répertoriées sont des espèces périphytiques communément considérées comme littorales, dont le développement important a été observé dans la zone pélagique du lac qui, sans doute, offre de nombreuses ressources et constitue un refuge vis-à-vis notamment de la prédation. L'absence de corrélations entre les communautés zooplanctoniques étudiés et les quelques variables physico-chimiques suivies, nous a amenés à considérer que le développement de ces communautés est sous la dépendance d'autres facteurs. D'ailleurs, une analyse canonique de correspondance indique que les stations, profondeurs et mois de prélèvement expliqueraient respectivement 3%, 5% et 36% de la variance totale associée à l'abondance des espèces prises en compte. Ce qui nous a permis d'émettre l'hypothèse selon laquelle les principales conditions environnementales qui affectent le développement potentiel des espèces analysées et le déroulement de leurs cycles biologiques, seraient dépendantes des deux saisons (la saison des pluies et la saison sèche) caractérisant les climats tropicaux.The rotifer and cladoceran communities of a small tropical eutrophic lake (the municipal lake of Yaoundé, Cameroon) were examined from November 1996 to December 1997, in relation to some physical and chemical variables. Using a Van Dorn bottle, water samples were collected weekly from 5 depths (0 m, 0.5 m, 1 m, 1.5 m and 2.5 m) at three stations (I, II and III) representing the upper, middle and lower parts of the lake, respectively. Water temperature was measured with a thermometer placed in the sampling bottle and pH was measured in the field with a portable pH-meter. Oxygen concentrations were determined according to the Winkler method. Water colour, suspended solids, and ammonium-nitrogen (NH4 -N) were analyzed spectrophotometrically using standard methods. The transparency of the water column was determined with a Secchi disk. Zooplankton species were identified alive under a phase contrast microscope using several manuals, and rotifers and cladocerans were counted in a Dolfuss chamberThroughout the study, temperature and pH values of the water remained around 25°C and 7, respectively. The Secchi disk transparency did not exceed 1 m and the dissolved oxygen concentration decreased with depth to almost zero in the bottom waters. Spatial and temporal variations of these variables indicated that the middle zone (0.5 and 1.5 m) was the stratum with a critical role in the functioning of the lake. Indeed, it represented the transition zone between the trophogenic upper water layer (0 to 0.5 m) where photosynthetic activity occurred and the bottom water layer (1.5 to 2.5 m) formed by the microaerophilic to anaerobic tropholitic layer where reducing processes were important.The rotifer and cladoceran communities were relatively diverse, with 41 species collected during the study year (i.e., 26 species of Brachionidae and Trichocercidae and 15 species of Cladocera), of which 16 are reported for the first time in Cameroon. These organisms filter small organic particles (fresh algal detritus and bacteria) and their presence in the lake could be related to the relatively high algal biomass found there. In terms of relative abundance, rotifers appear to be more important than cladocerans. Among the quantitatively dominant species, the most frequent ones were the Brachionidae (Brachionus angularis angularis, B. falcatus falcatus, B. calyciflorus), the Trichocercidae (Trichocerca elongata elongata and T. bicristata bicristata) and the Cladocera (Ceriodaphnia cornuta and Chydorus eurynotus).Brachionus angularis was the most abundant and frequent rotifer observed during this study. According to several authors, a high abundance of Brachionus can be considered as a biological indicator of more eutrophic waters. This corroborates the hypereutrophic status assigned to the lake through previous studies dealing with bacterioplankton and phytoplankton in this system. Among Cladocera, Daphnia, an important bacterivore, was absent despite the high density of bacteria (~108 cell mL-1) in the lake. Previous studies in other Cameroonian aquatic systems reported the scarcity of Daphnia and these earlier authors argued that it was probably replaced by smaller sized species, which in the case of the present study would be Ceriodaphnia cornuta and Moina micrura, the only representatives of Daphniidae encountered. Up to 75% of the number of species identified were periphytic species that generally develop in the littoral zone. The massive occurrence of these species in the plankton sample of the lake was related to the high particle content. Abundance generally peaked in the middle zone of the water column, likely due to the availability of resources and the general potential for such zones to provide a refuge from predation.However, there did not appear to be any correlation between the rotifer and cladoceran communities and physico-chemical variables when the data set was examined as whole, or grouped by seasons. This suggests that the heterogeneity in species abundances was controlled by other factors, among them sampling points, depths and time of sampling. A multivariate test using canonical correspondence analysis suggested that about 3% and 5% of the variance associated with the species abundance heterogeneity could be explained by sampling point and depth, respectively. Up to 36% of this variance was attributed to the sampling months. We therefore hypothesize that the environmental conditions affecting the development and biological cycles of the studied species are highly dependent on the prevalent seasons found in a tropical zone, i.e. the rainy and the dry seasons

Aquatic invertebrate fauna of wells in a tropical mountain climate, western Cameroon

International audienceThe present study, conducted between May 2012 and September 2013, aimed to determine the distribution of groundwater invertebrates in the Bamoun tableland, Cameroon. A total of 216 samples taken from 30 wells in four localities – Foumban, Foumbot, Kouoptamo and Magba – contained 80 invertebrate taxa belonging to Crustacea, Oligochaeta, Arachnida, Insecta, Nematoda, Tardigrada, and Gastropoda. The community was relatively rich, diverse, and was dominated by crustaceans. The distribution of groundwater fauna was influenced mainly by local factors, especially those related to the physical structure of wells such as the presence of a margin, a closing system, and total depth. At the regional scale, elevation and season were the only factors influencing the distribution of fauna. Further research needs to be conducted to highlight the relationship between groundwater invertebrates and human impacts in rural area