125 research outputs found
Association between a marker of sperm DNA damage and sperm indices in infertile males in Benin City, Nigeria: A cross-sectional study
Background: Studies have shown oxidative DNA damage is associated with male infertility.
Objective: This study determines the levels of 8-hydroxy-2’-deoxyguanosine (8-OHdG) and some markers of oxidative stress in seminal fluid of males investigated for infertility and men of proven fertility in Benin City, Nigeria.
Materials and Methods: Semen samples produced by self or assisted masturbation were analyzed by microscopic technique according to the World Health Organization guidelines. Thereafter, samples were centrifuged and seminal fluid plasma separated and stored at -20°C prior to assay for 8-OHdG and oxidative stress biomarkers. Based on the sperm concentration/count, the overall samples were grouped into the following categories: normospermia (n = 20), oligozoospermia (n = 30), and azoospermia (n = 20). The control group comprised of 30 age-matched males of proven fertility. The seminal fluid 8-OHdG, total antioxidant status, superoxide dismutase and malondialdehyde (MDA) were assayed through ELISA and spectrophotometric methods, respectively.
Results: Seminal plasma level of 8-OHdG and MDA were significantly higher (p = 0.01) in infertile subjects than controls. The mean levels of 8-OHdG and MDA in infertile subjects were higher in azoospermia than oligospermia than normospermia and so, was least in the normospermia. Conversely, the mean levels of total antioxidant status and superoxide dismutase were significantly lower (p = 0.01) in infertile than fertile the control male subjects with levels higher in normospermia than oligospermia and least in azoospermia. Moreover, the seminal 8-OHdG correlated negatively with sperm count (r = -0.359, p = 0.01), percent motility (r = -0.388, p = 0.04), and percent morphology (r = -0.327, p = 0.02).
Conclusion: The assessment of sperm DNA damage in addition to routine seminal fluid analysis may play an important role in specific diagnosis and management of male infertility.
Key words: Male, Semen, Antioxidants, Sperm count, DNA damage
Perancangan Sistem Suspensi untuk Simulasi Respon Dinamik ¼ Bodi Mobil
Gaya dan momen yang terjadi pada permukaan kontak ban dengan jalan berhubungan erat dengan tingkat kenyaman dan keamanan berkendaraan. Karakteristik dari gaya dan momen ini sangatlah dinamik karena dipengaruhi oleh banyak faktor. Beberapa faktor utamanya adalah beban kenderaan, kecepatan, besarnya steering angle dan elastisitas ban. Posisi permukaan kontak ban dengan jalan yang selalu berpindah pada saat kenderaan berjalan menyebabkan sulit untuk bisa mengamati respon dinamik ban serta sulit untuk mengukur gaya dan momen yang terjadi. Penelitian ini menitikberatkan pada perancangan model kontruksi sistem suspensi yang bisa mensimulasikan gerak dinamis dari ¼ bodi mobil untuk posisi bagian kanan depan. Kontruksi yang dirancang pada dasarnya terdiri dari dua bagian yaitu kontuksi bagian samping dan kontruksi bagian bawah. Kontruksi bagian samping merupakan representasi dari kontruksi bodi mobil yang mana bagian ini bisa bergerak translasi dalam arah vertical untuk mensimulasikan perubahan posisi bodi mobil akibat perubahan berat, maneuver maupun akibat gangguan permukaan jalan. Kontruksi bagian bawah berfungsi sebagai penggerak untuk memutar ban. Kontruksi bagian bawah ini dirancang untuk bisa berputar dalam posisi statis. Komponen yang berputar pada kontruksi bagian bawah ini akan meneruskan putaran ke ban sehingga roda mobil akan ikut berputar dalam posisi statis juga. Untuk memastikan semua komponen yang digunakan aman, maka dilakukan perhitungan dimensi dan perhitungan keuatan material yang digunakan pada kontruksi ini. Disamping perhitungan manual, perhitungan kekuatan material dan simulasi juga dilakukan dengan menggunakan software CATIA. Bahan untuk rangka kontruksi dipilih baja profil ST-37 dengan young modulus 200 GPa, yield strenght 240 Mpa dan memiliki dimensi 50 x 50 x 5mm. Hasil simulasi dengan software CATIA terlihat bahwa besarnya tegangan maksimum yang bekerja pada rangka adalah 10.9 N/mm2. Nilai ini lebih kecil dari tegangan geser izin 37N/mm2, sehingga dapat disimpulkan bahwa material ST 37 berbentuk siku dengan dimensi 50x50x5mm aman digunakan untuk kontruksi alat simulasi sistem suspensi in
Economic Empowerment for Santri By Making Pulp Paper in Dayah of Ulumuddin, Lhokseumawe
Ulumuddin foundation situated between the coastal area and forest area is surrounded bytraditional and industry plantation. The foundation has been holding some bording school education such as SDIT, MTs, MA, and SMK, and esspecially Traditional Islamic School (Dayah). Most of santri look like lack ofliving cost because they only hope the morney from their parent who is only pre whealfers’ family. Given thesecircumstances, an effort was needed to improve the santris’ life. A partnership in technology, mentoring andcapital provision was taken through the Ipteks bagi Masyarakat (IbM) Program. A design of big blender wasprepared and introduced to two group of santri. A group of ten santri was involved to the program of makingpulp system technology aimed to increase the partners’ revenue. The results of the cost analysis concluded thatthe rate of return was 30%; the Break Event Point (BEP) was Rp.7.500/kg pulp; and return of inverment periodwas 0.6 years in-service. Copyright © 2017 Department of Mechanical Engineering. All rights reserved
Seroprevalence of dirofilariasis in dogs in Sukabumi Regency
Dirofilariasis merupakan penyakit serius pada anjing dan bersifat zoonotik. Penelitian ini bertujuan untuk mengukur prevalensi dan menentukan faktor risiko dirofilariasis di Kabupaten Sukabumi. Sebanyak 152 sampel darah diambil dari anjing di dua kecamatan di Sukabumi yang memiliki perbedaan karakteristik topografi yaitu Kecamatan Jampang Tengan yang merupakan wilayah perbukitan dan Kecamatan Cisolok dengan karakteristik daerah pantai. Serum darah diperiksa menggunakan rapid test kit komersial CHW Ag Test Kit 2.0 ® Bionote untuk mendeteksi keberadaan antigen cacing dewasa Dirofilaria immitis. Hasil penelitian menunjukkan seroprevalensi dirofilariasis pada anjing di kabupaten Sukabumi adalah 20,4%. Daerah dengan karakteristik perbukitan memiliki tingkat prevalensi dirofilariasis yang lebih tinggi dibandingkan dengan daerah pantai. Kejadian dirofilariasis di Kabupaten Sukabumi tidak dipengaruhi jenis kelamin, cara pemeliharaan dan umur anjing.Dirofilariasis is a serious zoonotic disease in dogs. This study aimed to measure the prevalence and determine the risk factors of dirofilariasis in the Sukabumi Regency. A total of 152 blood samples were collected from dogs in two sub-districts in Sukabumi which have different topographic characteristics, namely Jampang Tengan District which is a hilly area, and Cisolok District which is a coastal area. Blood serum was examined using the commercial rapid test kit CHW Ag Test Kit 2.0 ® Bionote to detect the presence of the Dirofilaria immitis adult worm antigen. The results showed that the seroprevalence of dirofilariasis in dogs in the Sukabumi Regency was 20.4%. Areas with hilly characteristics have a higher prevalence of dirofilariasis than coastal areas do. The incidence of dirofilariasis in the Sukabumi Regency was not influenced by sex, method of keeping, or age of the dog
Desain Model Pembelajaran “Garuda” di Prodi S1 Pendidikan Matematika (suatu implementasi pembelajaran SKA dalam mendukung pelaksanaan KBK Prodi S1 Pendidikan Matematika FKIP Universitas Pekalongan)
In order to help the matheatics department of teacher’s training and education Faculty of pekalongan University in applying the competence based curiculum the writer designed a teaching method based on SCL, constructivism, and andragogy in May the 1st 2011. This model was named GARUDA which was the softened form of goal setting, andragogy, responsible for the job, understand the material, diciplinary learning and achive learning. The main objective of GARUDA was to make the learners able to master each competence stated in each lecture, so that they can be human investment and not merely human capital
PENGEMBANGAN PERANGKAT PEMBELAJARAN MATEMATIKA BERBASIS PENDIDIKAN MATEMATIKA REALISTIK DENGAN STRATEGI HIPNOSIS MATERI HIMPUNAN KELAS VII
Based on the fore research the RME approaches were one of the teaching approaches that could improve the students’ activeness, besides the theoretic approach showed that hypnosis was one of the ways to maximize the students’ brain works, so that RME by using Hypnosis was used by the researcher to solve this problem. This study is proposed to create some Mathematics teaching instruments based on valid RME, practice and effective. The teaching instruments developed are Syllabi, Lesson Plan, and students’ activities worksheets, teaching CD, Learning Test. The method used in this research is 4- D by Thiagarajan that has been modified into 3-D such as; (1) define, (2) design and (3) develop. The results of this research are; (1) the valuators said that the Mathematics teaching Instruments based on RME was valid; (2) the researchers said that the RME using Hypnosis strategy was good (in score average 58), the researchers’ responses to the instruments were positive (in score average 82%); and (3) the students’ proportion that achieved the standardized passing grade in each cycle was 80%; the last is the average score in their prestige and activeness in the first cycle was 38,7 and 58,5; in the second cycle 77,9 and 66,4; in the third cycle 79,7 and 68,1. The summary of this research was that the Mathematics teaching instrument based on RME on the sets concept was valid, practice and effective. The researcher suggests to the SMP teachers to apply the mathematics teaching based on RME using Hypnosis Strategy on the other mathematics concepts. Key words: Realistic Mathematic Education (RME), Hypnosis
Penilaian Kinerja Keuangan Koperasi di Kabupaten Pelalawan
This paper describe development and financial performance of cooperative in District Pelalawan among 2007 - 2008. Studies on primary and secondary cooperative in 12 sub-districts. Method in this stady use performance measuring of productivity, efficiency, growth, liquidity, and solvability of cooperative. Productivity of cooperative in Pelalawan was highly but efficiency still low. Profit and income were highly, even liquidity of cooperative very high, and solvability was good
Tinjauan Hukum terhadap Cyber Pornografi di Indonesia
Pengaturan tindak pidana pornografi dalam sistem hukum di Indonesia diawali pada pengaturan tindak pidana, diikuti dengan tindak pidana pornografi, dan diakhiri dengan hakikat pengaturan tindak pidana pornografi. Tindak pidana pornografi dalam sistem hukum pidana Indonesia antara lain diatur dalam Kitab Undang-Undang Hukum Pidana (KUHP) sebagai kejahatan terhadap kesusilaan yang telah diatur dalam Pasal 281 dan Pasal 282. Secara khusus pornografi diatur dalam Undang-Undang Pornografi Nomor 44 Tahun 2008 yang mengatur tentang tindak pidana pornografi yang merupakan tindak pidana kejahatan. UndangUndang Nomor 19 Tahun 2016 Perubahan Atas Undang-Undang Nomor 11 Tahun 2008 Tentang Informasi dan Transaksi (UU ITE), khususnya dalam penggunaan internet menyinggung masalah pornografi tapi terkait pada muatan yang melanggar kesusilaan. Terdapat sanksi terhadap tindak pidana pornografi baik pidana pokok maupun pidana tambahan sesuai dengan ketentuan yang berlak
Microbiota Composition, HSP70 and Caspase-3 Expression as Marker for Colorectal Cancer Patients in Aceh, Indonesia
Aim: to investigate the relationship between microbiota composition with HSP70 and Caspase-3 expressions in colon tissue as an initial study to develop the candidate for early detection of colorectal cancer for Indonesian patients. Methods: this is a cross-sectional study on 32 patients undergoing colonoscopy; 16 patients of colorectal cancer (CRC) while the other 16 patients are not (colitis and internal hemorrhoid). The composition of microbiota in stool samples was examined using 16S rRNA Denaturing Gradient Gel Electrophoresis (DDGE) while expression of HSP70 was examined by immunohistochemistry and Caspase-3 by using Haematoxylin-Eosin(HE) staining to determine the morphological changes in colon tissue. Results: analysis of PCR-DDGE shows a different composition of microbiota between patients with CRC and non-CRC. All CRC patients showed disappearance of dominant band from Bifidobacterium groups. Histological observation based on Inter Class Correlation (ICC) test from all slide showed a high scores (5.2-9.2) in CRC patients and low scores (1.7-2.4) in non-CRC patients. HSP70 expression was increased significantly in CRC patients with the highest percentage of 84%, while expression of caspase-3 decreased with the highest percentage of 21%. Statistical analysis showed that the incidence of colorectal cancer was associated with the expression of HSP 70 (p<0.001), and Caspase 3 (p<0.001). Conclusion: bifidobacterium is an important indicator for colorectal cancer patients that show disappearance of dominant band, while expression of HSP70 increased and the Caspase-3 expression decreased significantly
Pengembangan Media Pembelajaran Matematika Berbasis Kocerin (Kotak Cerdas Interaktif) Dengan Menggunakan Model Discovery Learning di SMP
Penelitian ini dilatar belakangi oleh kurangnya kemampuan guru dan siswa dalam menciptakan inovasi baru selama pembelajaran berlangsung sehingga yang didapat hanyalah kurangnya minat siswa selama proses pembelajaran dan itu mengakibatkan banyak siswa yang mendapat nilai dibawah KKM. Penelitian ini bertujuan untuk mengembangkan suatu media pembelajaran berupa modul pembelajaran matematika berbasis KOCERIN dengan model Discovery Learning pada materi pokok faktorisasi suku aljabar, sehingga menghasilkan media pembelajaran yang layak dan efektif digunakan selama proses pembelajaran, serta diharapkan dapat meningkatkan hasil belajar siswa. Jenis penelitian yang digunakan adalah penelitian Research and Development. Penelitian ini dilakukan dengan dua tahap. Tahap pertama untuk pengembangan produk menggunakan model ADDIE dan tahap kedua untuk menguji keefektifan produk melalui tes uji coba dengan desain eksperimen Posttest-Only Control Design. Sebelum diujicobakan di lapangan, modul pembelajaran matematika berbasis KOCERIN dengan model Discovery Learning terlebih dahulu dilakukan uji validasi oleh ahli media dan ahli materiselanjutnya dilakukan tanggapan siswa terhadap media dalam uji coba lapangan. Secara rata - rata hasil dari validasi ahli dan tangapan siswasebagian besar berkriteria sangat layak. Jadi media pembelajaran tersebut valid untuk diujicobakan. Uji keefektifan produk ditunjukkan dari hasil belajar kelompok eksperimen lebih baik dari kelompok kontrol, dengan menggunakan uji t satu pihak kanan diperoleh nila
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