Optimal combination of MYCN differential gene and cellular senescence gene predicts adverse outcomes in patients with neuroblastoma

IntroductionNeuroblastoma (NB) is a common extracranial tumor in children and is highly heterogeneous. The factors influencing the prognosis of NB are not simple.MethodsTo investigate the effect of cell senescence on the prognosis of NB and tumor immune microenvironment, 498 samples of NB patients and 307 cellular senescence-related genes were used to construct a prediction signature.ResultsA signature based on six optimal candidate genes (TP53, IL-7, PDGFRA, S100B, DLL3, and TP63) was successfully constructed and proved to have good prognostic ability. Through verification, the signature had more advantages than the gene expression level alone in evaluating prognosis was found. Further T cell phenotype analysis displayed that exhausted phenotype PD-1 and senescence-related phenotype CD244 were highly expressed in CD8+ T cell in MYCN-amplified group with higher risk-score.ConclusionA signature constructed the six MYCN-amplified differential genes and aging-related genes can be used to predict the prognosis of NB better than using each high-risk gene individually and to evaluate immunosuppressed and aging tumor microenvironment

LncRNA-NEAT1/miR-148a-3p axis regulates cell viability, apoptosis and autophagy through wnt/β-catenin signaling pathway in Breast Cancer

Purpose: Breast cancer has over the years been one of major acute carcinomas in women. This study investigated the fundamental mechanistic functions of the lncRNA-NEAT1/miR-148a-3p/Wnt/β-catenin axis in moderating cell viability, apoptosis and autophagy in Breast Cancer (BC). Methods: RT-qPCR measured expression of lncRNA NEAT1 and microRNA-148a-3p in human cell lines for Breast Cancer. Cell transfection upregulated or silenced the genes with CCK-8, western blot and FCM apoptosis assays determining the cellular growth, proliferation and protein expression related to autophagy biomarkers. Furthermore, luciferase assay analyzed the luciferase activity of lncRNA- NEAT1 and microRNA-148a-3p Results: The outcomes indicated that LncRNA-NEAT1 was upregulated in BC cell lines and promoted cell viability, autophagy and inhibited Apoptosis in BC cells. However, lncRNA-NEAT1 knockdown inhibited cell viability, autophagy and enhanced apoptosis. In addition, lncRNA-NEAT1 directly targeted microRNA-148a-3p. And, it was found that microRNA-148a-3p overturns the cellular viability, autophagy and inhibitory effects on Apoptosis imposed by lncRNA-NEAT1 overexpression. Lastly, overexpressed lncRNA-NEAT1 activated the Wnt/β-catenin regulatory network through sponging microRNA-148a-3p in BC cell lines. Conclusion: The present study showcased that lncRNA-NEAT1 could enhance tumor development in breast cancer via playing the role of molecular sponge to microRNA-148a-3p, and eventually hyper invigorating the Wnt/β-catenin regulatory network

Organic-inorganic hybrid quasi-2D perovskites incorporated with fluorinated additives for efficient and stable four-terminal tandem solar cells

Quasi-two-dimensional (2D) lead halide perovskites have emerged as promising candidates for improving the environmental stability of perovskite solar cells (PSCs). Herein, we report the preparation of a new quasi-2D perovskite by introducing a fluorine-containing additive [3-(trifluoromethyl)benzylammonium iodide (3-TFMBAI)] into Cs0.17FA0.83Pb(I0.83Br0.17)3. The moderate doping of 3-TFMBAI effectively induces the formation of the Ruddlesden-Popper perovskite phase, which can passivate the trap states and restrain the ionic motion in the perovskite lattice. The constructed 3-(trifluoromethyl)benzylamine molecular planes with strong hydrophobicity favorably suppress the decomposition and collapse of the perovskite phase against humidity. Moreover, the introduction of Cs+ and Br- ions tune the bandgap and improve the absorption, crystallinity and thermal stability of the perovskite films. As a result, a champion photoelectric conversion efficiency (PCE) of 20.89% is achieved, along with an improved open-circuit voltage reaching 1.22 V. The quasi-2D PSCs without encapsulation maintain 90.7% of the initial PCE after 1000 h under continuous heating at 60 °C and simultaneous exposure to humid air with a relative humidity of 60%. Four-terminal tandem solar cells are fabricated by combining top semi-transparent quasi-2D PSCs with bottom monocrystalline silicon solar cells, achieving an overall PCE of 23.53% and favorable performance stability