The potentiation effect of Bawang Dayak (Sisyrinchium palmifolium L.) extract on T47D cell growth inhibition after 5-fluorouracil treatment

5-Fluorouracil (5-FU) is chemotherapy agents that used in breast cancer treatment that has toxic effects such as myelotoxicity. its effectiveness relatively low. Bawang Dayak has been widely empirically used as a breast cancer treatment. This study's aims were to determine the sensitivity of 5-FU against T47D cells, as single and its combination with EBD, to determine apoptotic induction, and the inhibitory effect of T47D cell cycle. The cytotoxic assay was using the MTT method with the concetration of 5-FU (0-600μg / mL) and EBD (0-250μg / mL). Apoptotic induction analysis were determined by PI/annexin V. Both of apoptotic and cell cycle analysis was performed by flowcytometry. The results of the 5-FU and EBD cytotoxic test on T47D cells obtained IC50 274μg/mL and 75μg/mL, respectively. The combination of 5-FU and EBD has a mild to strong synergistic effect (CI 0.829 and 0.779). its ombined treatment induced apoptosis in T47D cells by 21.33%. Combination of these combinations caused cell accumulation in G0-G1 phase of 57.11%, greater than control (41.20%). Provision of 5-FU and EBD combination treatments causes disturbance and damage to cells that cause cell cycle arrest in the G0-G1 phase and can not proceed to the next phase. The combination of 5-fluorouracil and EBD has a synergistic effect as breast cancer co-chemotherapy is able to induce apoptosis against T47D cells by triggering cell accumulation in G0-G1 phase. The extract of Bawang Dayak has a high potential to be developed as co-chemotherapy in breast cancer

Pelatihan Pengendalian Risiko pada Keselamatan Kerja di Jasa Binatu

Laundry services are essential to support other industries, such as tourism and health. Hospitals, hotels, and restaurants are customers of the laundry industry. Occupational health and safety risks in the laundry sector have become a government concern through the Ministry of Health of the Republic of Indonesia issuing the Hospital Laundry Ergonomics Guide. This activity aims to provide knowledge regarding occupational safety risk control for the laundry service industry. Workers in this sector face risks of ergonomics and chemical hazards. Ergonomics risk comes from lifting and transporting linen material in sorting, washing, and drying activities up to the final process. Workers need to be given the insight to care about safety and how to control the hazards they face. By carrying out this activity, it is hoped that participants will become more aware of occupational safety risk control to improve their quality of life.Layanan binatu sangat penting sebagai penunjang industri lain seperti industri pariwisata dan kesehatan. Rumah sakit, hotel, dan restoran adalah pelanggan dari industri binatu. Risiko kesehatan dan keselamatan kerja di bidang binatu menjadi perhatian pemerintah melalui Kementerian Kesehatan Republik Indonesia dengan diterbitkannya Panduan Ergonomi Laundry Rumah Sakit. Tujuan dari kegiatan ini adalah untuk memberikan pengetahuan kepada pengelola dan pekerja jasa binatu mengenai pengendalian risiko keselamatan kerja. Pekerja di sektor ini menghadapi risiko bahaya ergonomi dan kimia. Risiko ergonomi berasal dari kegiatan angkat dan angkut material linen pada aktivitas sortir, pencucian, pengeringan sampai dengan proses akhirnya. Pekerja perlu diberikan wawasan untuk peduli pada keselamatan dan bagaimana mengendalikan bahaya yang dihadapinya. Dengan dilaksanakannya kegiatan ini, diperoleh hasil pengabdian bahwa peserta menjadi lebih memahami pengendalian risiko keselamatan kerja, sehingga kualias hidup mereka dapat meningkat

Hubungan Antara Shift Kerja, Masa Kerja Dengan Kelelahan Kerja Pada Perawat Daerah Surakarta Saat Pandemi Covid-19

The current end of the Covid-19 pandemic cannot be determined. The increasing number of patients in several hospitals has forced nurses to do more care for these patients. This study aims to determine the relationship between work shifts, length of work and work fatigue among nurses in the Surakarta area during the Covid-19 pandemic. This study used a descriptive analytic method with a sample of 30 nurses in Surakarta. The location was chosen because Surakarta has a fairly high rate of increase in cases infected with Covid-19. The data analysis method used is the independent t-test and chi square test. Based on the results of the independent t-test, it has a significance value of 0.025, which means there are differences in working hours before and after the Covid-19 pandemic. Chi-Square test at a value of 0.024 and 0.005 so that the work shift and length of work affect the work fatigue experienced by nurses. The results of the study also stated that there were differences in working hours for nurses. There was a relationship between work shifts and length of work on work fatigue experienced by nurses. It is better for the hospital management to be able to manage working time and rest time as best as possible so that nurses can remain optimal when working

Pengenalan Penggunaan Aplikasi Zoom pada Pembelajaran Daring Pelajaran IPA di SMP Islam Terpadu Al Ghazali Palangka Raya: Dampak Sikap Belajar

Pandemic COVID-19 caused many changes in life, including in the world of education. The learning process that has always been done face-to-face has now been implemented online with various video conferencing. One type of application that can be used as a learning medium is zoom meeting. This service activity was carried out at the Al Ghazali Integrated Islamic Middle School, Palangka Raya with the aim of describing the assessment of student attitudes in participating in online learning for science subjects. The attitude assessment developed is in the form of observations when students participate in online learning through the Zoom Meeting

MODUL TOT MADRASAH PEREMPUAN BERKEMAJUAN

Pada modul ini diberikan beberapa contoh metode atau tekhnik penyampaian materi yang beragam, namun demikian fasilitator dipersilahkan improvisasi penyesuaian metode penyampaian agar relevan dengan situasi dan kondisi peserta pelatihan. Penguasaan materi oleh fasilitator adalah hal yang sangat penting, utamanya pada sesi 1 sampai 5 yang merupakan inti daripada Madrasah Perempuan Berkemajuan. Penyampaian materi sesi 6 sampai 9 dapat disesuaikan dengan relevansi kebutuhan peserta pelatihan. Tentu saja modul ini masih banyak kekurangan, ikhtiar positif senantiasa kita lakukan untuk ikut berkontribusi melakukan perubahan warga persyariakatan dan masyarakat Indonesia pada umumnya. Karena itu kami terbuka jika ada masukan-masukan kontruktif demi penyempurnaan modul ini