A sputtering derived atomic oxygen source for studying fast atom reactions

A technique for the generation of fast atomic oxygen was developed. These atoms are created by ion beam sputtering from metal oxide surfaces. Mass resolved ion beams at energies up to 60 KeV are produced for this purpose using a 150 cm isotope separator. Studies have shown that particles sputtered with 40 KeV Ar(+) on Ta2O5 were dominantly neutral and exclusively atomic. The atomic oxygen also resided exclusively in its 3P ground state. The translational energy distribution for these atoms peaked at ca 7 eV (the metal-oxygen bond energy). Additional measurements on V2O5 yielded a bimodal distribution with the lower energy peak at ca 5 eV coinciding reasonably well with the metal-oxygen bond energy. The 7 eV source was used to investigate fast oxygen atom reactions with the 2-butene stereoisomers. Relative excitation functions for H-abstraction and pi-bond reaction were measured with trans-2-butene. The abstraction channel, although of minor relative importance at thermal energy, becomes comparable to the addition channel at 0.9 eV and dominates the high-energy regime. Structural effects on the specific channels were also found to be important at high energy

THE USE OF UNI-AXIAL GYROSCOPE FOR MONITORING HEEL TILTING VELOCITY DURING SIMULATED ANKLE SUPINATION SPRAIN MOTIONS

This study investigated the feasibility of using a uni-axial gyroscope to monitor the motion of foot segment. Five male subjects performed supination spraining motion simulated by a mechanical sprain simulator. A uni-axial gyroscope was attached on the shoe surface at the heel position of the right shoe to collect the heel tilting velocity. Optical motion analysis was also used to obtain heel tilting velocity as a standard. The intra class correlation and root mean square error of tilting velocity measured by the two methods are 0.70 – 0.99 and 8.21 – 37.11 deg/s, respectively. The result shows that it is possible to use only one uni-axial gyroscope for monitoring foot segment motion. This monitoring method can be contributed to the currently developing active protection “sprain-free shoe”

THE EFFECT OF MYOELECTRIC STIMULATION ON PERONEAL MUSCLES TO RESIST SUDDEN SIMULATED ANKLE SPRAIN MOTIONS

This study evaluated the effect of myoelectric stimulation on peroneal muscles to resist sudden simulated ankle sprain motions. Ten male subjects performed unanticipated inversion and supination spraining motions simulated by a mechanical sprain simulator. Myoelectric stimulations with different delay time were delivered to the peroneal muscles to initiate involuntary muscle contraction and ankle joint pronation torque to resist the spraining motion. The motion was captured and analyzed by a motion analysis system, and was quantified by the reduction of maximum heel tilting angle and angular velocity. Results showed significant effect in all conditions with the myoelectric stimulation of any delay time within 15ms. The maximum heel tilting angle and angular velocity dropped from 18 to 9-13 degrees and from 200-250 to 140-170 degree/s respectively. The present corrective mechanism could be implemented in our current research to develop an intelligent sprain-free sport shoe attempting to prevent ankle sprain injury in sports

OMMA enables population-scale analysis of complex genomic features and phylogenomic relationships from nanochannel-based optical maps.

BackgroundOptical mapping is an emerging technology that complements sequencing-based methods in genome analysis. It is widely used in improving genome assemblies and detecting structural variations by providing information over much longer (up to 1 Mb) reads. Current standards in optical mapping analysis involve assembling optical maps into contigs and aligning them to a reference, which is limited to pairwise comparison and becomes bias-prone when analyzing multiple samples.FindingsWe present a new method, OMMA, that extends optical mapping to the study of complex genomic features by simultaneously interrogating optical maps across many samples in a reference-independent manner. OMMA captures and characterizes complex genomic features, e.g., multiple haplotypes, copy number variations, and subtelomeric structures when applied to 154 human samples across the 26 populations sequenced in the 1000 Genomes Project. For small genomes such as pathogenic bacteria, OMMA accurately reconstructs the phylogenomic relationships and identifies functional elements across 21 Acinetobacter baumannii strains.ConclusionsWith the increasing data throughput of optical mapping system, the use of this technology in comparative genome analysis across many samples will become feasible. OMMA is a timely solution that can address such computational need. The OMMA software is available at https://github.com/TF-Chan-Lab/OMTools

Estimation of mutation rates in cultured mammalian cells

The factors that affect reliable estimations of mutation rates ([mu]) in cultured mammalian somatic cell populations by fluctuation analysis are studied experimentally and statistically. We analyze the differential effect of the final cell population size in each culture (Nt) and the number of parallel cultures (C) on the variation in the rate estimates () inferred from the P0 method. The analysis can be made after the derivation of the variance of , which is a measure of variation of for a given combination of Nt and C in a number of repeat experiments. The variance of is inversely proportional to C and to the square of Nt. Nt determines the probability of occurrence of mutation in a cell culture. By influencing the size of P0, Nt also determines whether a rate estimate is obtainable from the experiment. Since Po is estimated from the fraction of cultures containing no mutation in a set of C cultures, C becomes a determining factor for the accuracy of . The rate estimated from is biased, but the bias is in general 2 orders of magnitude smaller than . By the selection of an appropriate combination of Nt and C for the experiment, this bias can be reduced even further.Based on the notion of comparing two proportions, we propose a test statistic and have applied it to experimental results for a test of equality of mutation rates in different cell lines. This development places the comparison of mutation rates on a statistical basis.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/25103/1/0000535.pd

Differential Presentations of Arterial Thromboembolic Events Between Venous Thromboembolism and Atrial Fibrillation Patients

Objective: Atrial fibrillation (AF) and venous thromboembolism (VTE) share several risk factors related to arterial thromboembolism. No study has reported the differential contribution to arterial thromboembolic events and mortality between these two conditions in the same population. We therefore assessed the differential arterial thromboembolic events between AF and VTE. Methods: We included AF and VTE national cohorts derived from Taiwan National Health Insurance Research Database between 2001 and 2013. The eligible population was 314,861 patients in the AF cohort and 41,102 patients in the VTE cohort. The primary outcome was arterial thromboembolic events, including ischemic stroke, extracranial arterial thromboembolism (ECATE) and myocardial infarction (MI). Secondary outcomes were all-cause mortality and cardiovascular death. Results: After a 1:1 propensity matching, 32,688 patients in either group were analyzed. The risk of arterial thromboembolic events was lower in the VTE cohort than that in the AF cohort (subdistribution hazard ratio [SHR], 0.60; 95% confidence interval [CI], 0.57–0.62). The risk of ischemic stroke (SHR, 0.44; 95% CI, 0.42–0.46) and MI (SHR, 0.80; 95% CI, 0.72–0.89) were lower in the VTE cohort, while the risk of ECATE (SHR, 1.23; 95% CI, 1.14–1.33; particularly lower extremities) was higher in the VTE cohort. All-cause mortality rate was higher in the VTE cohort (HR, 1.18; 95% CI, 1.15–1.21) while the risk of cardiovascular death was lower in the VTE cohort (HR, 0.96; 95% CI, 0.93–0.995). Conclusions: Patients with AF had higher risks of arterial thromboembolic events compared to patients with VTE, despite having risk factors in common. The VTE cohort had higher risks of all-cause mortality and ECATE, particularly lower extremity events, compared to AF patients. The differential manifestations of thromboembolism sequelae and mortality between AF and VTE patients merit further investigation

Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia

Background. Recently, we described the discovery of a novel group 2 coronavirus, coronavirus HKU1 (CoV-HKU1), from a patient with pneumonia. However, the clinical and molecular epidemiological features of CoV-HKU1-associated pneumonia are unknown. Methods. Prospectively collected (during a 12-month period) nasopharyngeal aspirates (NPAs) from patients with community-acquired pneumonia from 4 hospitals were subjected to reverse-transcription polymerase chain reaction, for detection of CoV-HKU1. The epidemiological, clinical, and laboratory characteristics of patients with CoV-HKU1-associated pneumonia were analyzed. The pol, spike (S), and nucleocapsid (N) genes were also sequenced. Results. NPAs from 10 (2.4%) of 418 patients with community-acquired pneumonia were found to be positive for CoV-HKU1. All 10 cases occurred in spring and winter. Nine of these patients were adults, and 4 had underlying diseases of the respiratory tract. In the 6 patients from whom serum samples were available, all had a 4-fold change in immunoglobulin (Ig) G titer and/or presence of IgM against CoV-HKU1. The 2 patients who died had significantly lower hemoglobin levels, monocyte counts, albumin levels, and oxygen saturation levels on admission and had more-extensive involvement visible on chest radiographs. Sequence analysis of the pol, S, and N genes revealed 2 genotypes of CoV-HKU1. Conclusions. CoV-HKU1 accounts for 2.4% of community-acquired pneumonia, with 2 genotypes in the study population. Without performance of diagnostic tests, the illness was clinically indistinguishable from other community-acquired pneumonia illnesses. © 2005 by the Infectious Diseases Society of America. All rights reserved.published_or_final_versio

Screening of the topological charge in a correlated instanton vacuum

Screening of the topological charge due to he fermion-induced interactions is an important phenomenon, closely related with the resolution of the strong CP and U(1) problems. We study the mechanism of such screening in a 'correlated instanton vacuum', as opposed to the 'random' one. Both scalar and pseudoscalar gluonic correlators are analyzed by means of an observable that minimizes finite size effects. Screening of the topological charge is established. This allows us to calculate the $\eta'$ mass without having to invert the Dirac operator. We suggest that this method might be used in lattice QCD calculations as well. Our results for the screening of the topological charge are in agreement with the chiral Ward identities, and the scalar gluonic correlator satisfies a low energy theorem first derived by Novikov et al. \cite{Novikov-etal}. We also propose to evaluate the topological susceptibility in the Witten-Veneziano formula not in an infinite box in an world $without$ fermions but in an infinitesimal box in a world $with$ fermions.Comment: 22 pages + 5 postscript figures, SUNY-NTG/94-25. Corrected LATEX erro

The instanton liquid in QCD at zero and finite temperature

In this paper we study the statistical mechanics of the instanton liquid in QCD. After introducing the partition function as well as the gauge field and quark induced interactions between instantons we describe a method to calculate the free energy of the instanton system. We use this method to determine the equilibrium density and the equation of state from numerical simulations of the instanton ensemble in QCD for various numbers of flavors. We find that there is a critical number of flavors above which chiral symmetry is restored in the groundstate. In the physical case of two light and one intermediate mass flavor the system undergoes a chiral phase transition at $T\simeq 140$ MeV. We show that the mechanism for this transition is a rearrangement of the instanton liquid, going from a disordered, random, phase at low temperatures to a strongly correlated, molecular, phase at high temperature. We also study the behavior of mesonic susceptibilities near the phase transition.Comment: 50 pages, revtex, 16 figures, uuencode

The Yuan-Tseh Lee Array for Microwave Background Anisotropy

The Yuan-Tseh Lee Array for Microwave Background Anisotropy (AMiBA) is the first interferometer dedicated to studying the cosmic microwave background (CMB) radiation at 3mm wavelength. The choice of 3mm was made to minimize the contributions from foreground synchrotron radiation and Galactic dust emission. The initial configuration of seven 0.6m telescopes mounted on a 6-m hexapod platform was dedicated in October 2006 on Mauna Loa, Hawaii. Scientific operations began with the detection of a number of clusters of galaxies via the thermal Sunyaev-Zel'dovich effect. We compare our data with Subaru weak lensing data in order to study the structure of dark matter. We also compare our data with X-ray data in order to derive the Hubble constant.Comment: accepted for publication in ApJ (13 pages, 7 figures); a version with high resolution figures available at http://www.asiaa.sinica.edu.tw/~keiichi/upfiles/AMiBA7/pho_highreso.pd