Circumferential scouting punch biopsies to delineate surgical margin for dermatofibrosarcoma protuberans

AbstractDermatofibrosarcoma protuberans (DFSP) is an uncommon soft-tissue tumor involving the dermis and subcutaneous tissue with a high local recurrence rate after standard excision. Mohs micrographic surgery offers a lower recurrence rate. However, the procedure requires multiple stages of excision with intraoperative histopathological mapping, which is time consuming and expensive. We report our experience of using circumferential scouting punch biopsy technique in five patients to determine in advance the resection margins for DFSP prior to wide excision. Multiple 4 mm punches, usually eight in number, were performed 1–2.5 cm around the palpable borders of DFSP to delineate the resection margins in five consecutive patients. Tumors were excised at a later date along the margin defined by these biopsies and the wounds were repaired with skin graft. The operation was completed in 2 hours in all cases excluding one that required frozen sections for deep margin. No recurrence was noted 2–10 years after the operations. The results suggest that circumferential scouting punch biopsies before wide excision may be an alternative method to define the resection margins for DFSP when Mohs surgery is not available

Critical Behavior of Frustrated Josephson Junction Arrays with Bond Disorder

The scaling behavior of the current-voltage ($IV$) characteristics of a two-dimensional proximity-coupled Josephson junction array (JJA) with quenched bond disorder was investigated for frustrations $f=1/5$, 1/3, 2/5, and 1/2. For all these frustrations including 1/5 and 2/5 where a strongly first-order phase transition is expected in the absence of disorder, the $IV$ characteristics exhibited a good scaling behavior. The critical exponent $\nu$ indicates that bond disorder may drive the phase transitions of frustrated JJA's to be continuous but not into the Ising universality class, contrary to what was observed in Monte Carlo simulations. The dynamic critical exponent $z$ for JJA's was found to be only 0.60 - 0.77.Comment: RevTeX4, 4 pages, 4 figures, the manuscript is replaced with the published versio

Enhanced Efficiency and Stability of Planar Perovskite Solar Cells Using a Dual Electron Transport Layer of Gold Nanoparticles Embedded in Anatase TiO2 Films

Incorporating plasmonic nanostructures is a promising strategy to enhance both the optical and electrical characteristics of photovoltaic devices via more efficient harvesting of incident light. Herein, we report a facile fabrication scheme at low temperature for producing gold nanoparticles embedded in anatase TiO2 films, which can simultaneously improve the efficiency and stability of n-i-p planar heterojunction perovskite solar cells (PSCs). The PSCs based on rigid and flexible substrates with 0.2 wt % Au-TiO2/TiO2 dual electron transport layers (ETLs) achieved power conversion efficiencies up to 20.31 and 15.36%, superior to that of devices with TiO2 as a single ETL. Moreover, 0.2 wt % Au-TiO2/TiO2 devices demonstrated significant stability in light soaking, which is attributed to improved light absorption, low charge recombination loss, and enhanced carrier transport, and extraction with the plasmonic Au-TiO2/TiO2 dual ETL. The present work improves the practicability of high-performance and flexible PSCs by engineering the photogenerated carrier dynamics at the interface

Frustrated two-dimensional Josephson junction array near incommensurability

To study the properties of frustrated two-dimensional Josephson junction arrays near incommensurability, we examine the current-voltage characteristics of a square proximity-coupled Josephson junction array at a sequence of frustrations f=3/8, 8/21, 0.382 $(\approx (3-\sqrt{5})/2)$, 2/5, and 5/12. Detailed scaling analyses of the current-voltage characteristics reveal approximately universal scaling behaviors for f=3/8, 8/21, 0.382, and 2/5. The approximately universal scaling behaviors and high superconducting transition temperatures indicate that both the nature of the superconducting transition and the vortex configuration near the transition at the high-order rational frustrations f=3/8, 8/21, and 0.382 are similar to those at the nearby simple frustration f=2/5. This finding suggests that the behaviors of Josephson junction arrays in the wide range of frustrations might be understood from those of a few simple rational frustrations.Comment: RevTex4, 4 pages, 4 eps figures, to appear in Phys. Rev.

Systemic DKK1 neutralization enhances human adipose-derived stem cell mediated bone repair

: Progenitor cells from adipose tissue are able to induce bone repair; however, inconsistent or unreliable efficacy has been reported across preclinical and clinical studies. Soluble inhibitory factors, such as the secreted Wnt signaling antagonists Dickkopf-1 (DKK1), are expressed to variable degrees in human adipose-derived stem cells (ASCs), and may represent a targetable "molecular brake" on ASC mediated bone repair. Here, anti-DKK1 neutralizing antibodies were observed to increase the osteogenic differentiation of human ASCs in vitro, accompanied by increased canonical Wnt signaling. Human ASCs were next engrafted into a femoral segmental bone defect in NOD-Scid mice, with animals subsequently treated with systemic anti-DKK1 or isotype control during the repair process. Human ASCs alone induced significant but modest bone repair. However, systemic anti-DKK1 induced an increase in human ASC engraftment and survival, an increase in vascular ingrowth, and ultimately improved bone repair outcomes. In summary, anti-DKK1 can be used as a method to augment cell-mediated bone regeneration, and could be particularly valuable in the contexts of impaired bone healing such as osteoporotic bone repair

HAWC+/SOFIA Polarimetry in L1688: Relative Orientation of Magnetic Field and Elongated Cloud Structure

We present a study of the relative orientation between the magnetic field and elongated cloud structures for the $\rho$ Oph A and $\rho$ Oph E regions in L1688 in the Ophiuchus molecular cloud. Combining inferred magnetic field orientation from HAWC+ 154 $\mu$m observations of polarized thermal emission with column density maps created using Herschel submillimeter observations, we find consistent perpendicular relative alignment at scales of $0.02$ pc ($33.6"$ at $d \approx 137$ pc) using the histogram of relative orientations (HRO) technique. This supports the conclusions of previous work using Planck polarimetry and extends the results to higher column densities. Combining this HAWC+ HRO analysis with a new Planck HRO analysis of L1688, the transition from parallel to perpendicular alignment in L1688 is observed to occur at a molecular hydrogen column density of approximately $10^{21.7}$ cm$^{-2}$. This value for the alignment transition column density agrees well with values found for nearby clouds via previous studies using only Planck observations. Using existing turbulent, magnetohydrodynamic simulations of molecular clouds formed by colliding flows as a model for L1688, we conclude that the molecular hydrogen volume density associated with this transition is approximately $\sim10^{4}$ cm$^{-3}$. We discuss the limitations of our analysis, including incomplete sampling of the dense regions in L1688 by HAWC+.Comment: To be published in Ap

DNA resection in eukaryotes: deciding how to fix the break

DNA double-strand breaks are repaired by different mechanisms, including homologous recombination and nonhomologous end-joining. DNA-end resection, the first step in recombination, is a key step that contributes to the choice of DSB repair. Resection, an evolutionarily conserved process that generates single-stranded DNA, is linked to checkpoint activation and is critical for survival. Failure to regulate and execute this process results in defective recombination and can contribute to human disease. Here, I review recent findings on the mechanisms of resection in eukaryotes, from yeast to vertebrates, provide insights into the regulatory strategies that control it, and highlight the consequences of both its impairment and its deregulation

A Phenome-Based Functional Analysis of Transcription Factors in the Cereal Head Blight Fungus, Fusarium graminearum

Fusarium graminearum is an important plant pathogen that causes head blight of major cereal crops. The fungus produces mycotoxins that are harmful to animal and human. In this study, a systematic analysis of 17 phenotypes of the mutants in 657 Fusarium graminearum genes encoding putative transcription factors (TFs) resulted in a database of over 11,000 phenotypes (phenome). This database provides comprehensive insights into how this cereal pathogen of global significance regulates traits important for growth, development, stress response, pathogenesis, and toxin production and how transcriptional regulations of these traits are interconnected. In-depth analysis of TFs involved in sexual development revealed that mutations causing defects in perithecia development frequently affect multiple other phenotypes, and the TFs associated with sexual development tend to be highly conserved in the fungal kingdom. Besides providing many new insights into understanding the function of F. graminearum TFs, this mutant library and phenome will be a valuable resource for characterizing the gene expression network in this fungus and serve as a reference for studying how different fungi have evolved to control various cellular processes at the transcriptional level

Photocatalytic fabrics based on reduced graphene oxide and TiO2 coatings

Supplementary data associated with this article can be found, in the online version, at: http://dx.doi.org/10.1016/j.mseb.2015.04.013The purpose of this work is to obtain photocatalytic fabrics based on reduced graphene oxide (RGO) and TiO2 coatings on polyester fabrics. The influence of the applied number of RGO coatings on properties such as light absorption, conductivity, electroactivity and photocatalytic properties of the fabrics was established. An improvement of these properties with the number of RGO coatings applied was obtained. FESEM, EDX, XPS and FTIR-ATR showed the incorporation of the TiO2 nanoparticles on the fabrics. FTIR-ATR showed the formation of a bidentate carboxylic ligand with titanium atoms. The photocatalytic properties of the fabrics were tested with Rhodamine B dye solutions. Photocatalytic efficiency increased with the number of RGO coatings, due to the increased light absorption, and better electrical properties. The charge transfer resistance (Rct) and its time constant (τ) decreased, indicating a better electron transfer which helps to increase the lifetime of the pair electron/hole.Authors wish to thank to the Spanish Ministerio de Ciencia e Innovación (contract CTM2011-23583) for the financial sup port. J. Molina is grateful to the Conselleria d’Educació, Formació i Ocupació (Generalitat Valenciana) for the Programa VALi+D Postdoctoral Fellowship. Electron Microscopy Service of the UPV (Universitat Politècnica de València) is gratefully acknowledged for help with FESEM and EDX characterization. Timothy Vickers is gratefully acknowledged for help with English revision.info:eu-repo/semantics/publishedVersio

Elevated BCRP/ABCG2 Expression Confers Acquired Resistance to Gefitinib in Wild-Type EGFR-Expressing Cells

The sensitivity of non-small cell lung cancer (NSCLC) patients to EGFR tyrosine kinase inhibitors (TKIs) is strongly associated with activating EGFR mutations. Although not as sensitive as patients harboring these mutations, some patients with wild-type EGFR (wtEGFR) remain responsive to EGFR TKIs, suggesting that the existence of unexplored mechanisms renders most of wtEGFR-expressing cancer cells insensitive.Here, we show that acquired resistance of wtEGFR-expressing cancer cells to an EGFR TKI, gefitinib, is associated with elevated expression of breast cancer resistance protein (BCRP/ABCG2), which in turn leads to gefitinib efflux from cells. In addition, BCRP/ABCG2 expression correlates with poor response to gefitinib in both cancer cell lines and lung cancer patients with wtEGFR. Co-treatment with BCRP/ABCG2 inhibitors enhanced the anti-tumor activity of gefitinib.Thus, BCRP/ABCG2 expression may be a predictor for poor efficacy of gefitinib treatment, and targeting BCRP/ABCG2 may broaden the use of gefitinib in patients with wtEGFR