33 research outputs found
2D-BN nanoparticles as a spectroscopic marker and drug delivery system with protection properties
An application of 2D-BN nanoparticles as a spectroscopic marker, weak luminescent marker and anticancer drug (doxorubicin, DOX) delivery system with protection properties was studied for the LNCaP strains of cancer cells using FTIR and Raman spectroscopy for analysing the cancer cells, cells with BN, the cancer cells with DOX, and the cancer cells with BN nanoparticles loaded by DOX. Study of IR absorption and Raman spectra of the LNCaP strains of cancer cells incubated with 2D-BN nanoparticles for 1 hour showed that the 2D-BN nanoparticles could pass through the cell membrane and localize inside the membrane or close to the membrane in the cytoplasm of the cells. We registered the spectra of the disturbed lipids during the DOX-2D-BN passing through the membrane. After incubation for 2 hours and more, spectral changes in other structural components of the cell (nuclei, cytoplasm, mitochondria) can be registered. Confocal microscopy showed that a gold nanostructured support enhances the fluorescence of the cancer cells with 2D-BN as well as that with DOX, however the double action of 2D-BN and DOX on the cancer cells aggravates the emission property of the studied system. An MTT test showed that the toxicity of DOX on the 2D-BN nanoparticles is less than that on the reference cells, and at the same time the efficiency of the DOX action on the cancer cells does not change
Optical properties of graphene oxide coupled with 3D opal based photonic crystal
International audienc
Organic Solvent-Free, One-Step Engineering of Graphene-Based Magnetic-Responsive Hybrids Using Design of Experiment-Driven Mechanochemistry
In this study, we propose an organic
solvent-free, one-step mechanochemistry approach to engineer water-dispersible
graphene oxide/superparamagnetic iron oxide (GO/SPIOs) hybrids, for
biomedical applications. Although mechanochemistry has been proposed
in the graphene field for applications such as drug loading, exfoliation
or polymer-composite formation, this is the first study to report
mechanochemistry for preparation of GO/SPIOs hybrids. The statistical
design of experiment (DoE) was employed to control the process parameters.
DoE has been used to control formulation processes of other types
of nanomaterials. The implementation of DoE for controlling the formulation
processes of graphene-based nanomaterials is, however, novel. DoE
approach could be of advantage as one can tailor GO-based hybrids
of predicted yields and compositions. Hybrids were characterized by
TEM, AFM FT-IR, Raman spectroscopy, and TGA. The dose鈥搑esponse
magnetic resonance (MR) properties were confirmed by MR imaging of
phantoms. The biocompatibility of the hybrids with A549 and J774 cell
lines was confirmed by the modified LDH assay