Determination of optimum feeding rate expressed as percent of body weight in Persian sturgeon, Acipenser persicus

The present study was conducted to determine the best feeding rates expressed as percent of body weight in Persian sturgeon fingerlings at different body weights. Eighteen fiberglass tanks each holding 30 fingerlings with an average initial body weight of 19.45 ± 0.5 g were used in this experiment. Six different feeding trials (1, 2, 3, 4, 5 and 6 percent of body weight) were employed and each trial was run in three replicates through a period of 60 days (4 periods of 15 days). The results were analyzed using Statgragh software and compared using Duncan test. The optimum recommended feeding rate was 4 percent of body weight for an increase of body weight from 19.5 to 31g and 3 percent of body weight for an increase of body weight from 33 to 47g. For an increase in body weight from 47 to 90g the recommended feeding rate was 3 percent of body weight. The recommended doses are subject to alteration with variation in water temperature

Integrated approach for zonation of a mid-Cenomanian carbonate reservoir in a sequence stratigraphic framework

The mid-Cenomanian Mishrif Formation (Fm.) is considered as one of the most important rudist-bearing reservoir horizons in the Sirri Oil Fields of the Persian Gulf. Due to the general heterogeneity of carbonate reservoirs, the use of an integrated approach is helpful for investigating porosity and permeability distribution along with recognizing controlling pore system factors in the reservoir. Thus, for the reservoir characterization of the Mishrif Fm., an integrated approach including facies analysis, diagenetic history and sequence stratigraphic analysis is considered. Detailed petrographic studies showed a total of eight microfacies and seven facies belts, related to inner ramp to the basin of a homoclinal carbonate ramp. Humid climatic condition and tectonic activity, associated with eustatic sea-level fluctuations during the mid-Cretaceous, led to meteoric diagenesis of the Mishrif carbonates during subaerial exposures (mid-Cenomanian and Cenomanian-Turonian disconformities). General diagenetic overprints and modifications include micritization, cementation, dissolution, compaction, dolomitization, pyritization and fracturing. Considering this reservoir in the sequence stratigraphic framework reveals that the reservoir zones development is basically related to the Cenomanian–Turonian sequence boundary, recognized in the three studied wells, and also to the mid-Cenomanian boundary, identified only in one well. In addition, pore system properties were inspected by differentiation of Hydraulic Flow Units (HFUs) within the reservoir. The identified flow units, based on their capability for fluid flow, can be classified into four main rock types with very high- (HFUD), high- (HFUC), medium- (HFUB) and low-quality (HFUA). Accordingly, this study shows that the main part of the Mishrif Reservoir is affected by diagenetic processes related to subaerial exposures, resulting in zones with higher storage capacity and fluid flow rates. So, the study of depositional and diagenetic characteristics of the Mishrif carbonates in the sequence stratigraphy framework is essential to unravel the reservoir heterogeneity, and to describe the reservoir zones and their distribution in the field and regional scale. In addition, observed changes in the thickness of hydrocarbon column are attributed to the different location of the studied wells on the anticline structures, which show a tilted oil-water contact with a slope to the North

Integrated approach for zonation of a mid-Cenomanian carbonate reservoir in a sequence stratigraphic framework

The mid-Cenomanian Mishrif Formation (Fm.) is considered as one of the most important rudist-bearing reservoir horizons in the Sirri Oil Fields of the Persian Gulf. Due to the general heterogeneity of carbonate reservoirs, the use of an integrated approach is helpful for investigating porosity and permeability distribution along with recognizing controlling pore system factors in the reservoir. Thus, for the reservoir characterization of the Mishrif Fm., an integrated approach including facies analysis, diagenetic history and sequence stratigraphic analysis is considered. Detailed petrographic studies showed a total of eight microfacies and seven facies belts, related to inner ramp to the basin of a homoclinal carbonate ramp. Humid climatic condition and tectonic activity, associated with eustatic sea-level fluctuations during the mid-Cretaceous, led to meteoric diagenesis of the Mishrif carbonates during subaerial exposures (mid-Cenomanian and Cenomanian-Turonian disconformities). General diagenetic overprints and modifications include micritization, cementation, dissolution, compaction, dolomitization, pyritization and fracturing. Considering this reservoir in the sequence stratigraphic framework reveals that the reservoir zones development is basically related to the Cenomanian-Turonian sequence boundary, recognized in the three studied wells, and also to the mid-Cenomanian boundary, identified only in one well. In addition, pore system properties were inspected by differentiation of Hydraulic Flow Units (HFUs) within the reservoir. The identified flow units, based on their capability for fluid flow, can be classified into four main rock types with very high- (HFUD), high- (HFUC), medium- (HFUB) and low-quality (HFUA). Accordingly, this study shows that the main part of the Mishrif Reservoir is affected by diagenetic processes related to subaerial exposures, resulting in zones with higher storage capacity and fluid flow rates. So, the study of depositional and diagenetic characteristics of the Mishrif carbonates in the sequence stratigraphy framework is essential to unravel the reservoir heterogeneity, and to describe the reservoir zones and their distribution in the field and regional scale. In addition, observed changes in the thickness of hydrocarbon column are attributed to the different location of the studied wells on the anticline structures, which show a tilted oil-water contact with a slope to the Nort

TACAN is an essential component of the mechanosensitive ion channel responsible for pain sensing

Mechanotransduction, the conversion of mechanical stimuli into electrical signals, is a fundamental process underlying several physiological functions such as touch and pain sensing, hearing and proprioception. This process is carried out by specialized mechanosensitive ion channels whose identities have been discovered for most functions except pain sensing. Here we report the identification of TACAN (Tmem120A), an essential subunit of the mechanosensitive ion channel responsible for sensing mechanical pain. TACAN is expressed in a subset of nociceptors, and its heterologous expression increases mechanically-evoked currents in cell lines. Purification and reconstitution of TACAN in synthetic lipids generates a functional ion channel. Finally, knocking down TACAN decreases the mechanosensitivity of nociceptors and reduces behavioral responses to mechanical but not to thermal pain stimuli, without affecting the sensitivity to touch stimuli. We propose that TACAN is a pore-forming subunit of the mechanosensitive ion channel responsible for sensing mechanical pain

New FTY720-docetaxel nanoparticle therapy overcomes FTY720-induced lymphopenia and inhibits metastatic breast tumour growth

Purpose: Combining molecular therapies with chemotherapy may offer an improved clinical outcome for chemoresistant tumours. Sphingosine-1-phosphate (S1P) receptor antagonist and sphingosine kinase 1 (SK1) inhibitor FTY720 (FTY) has promising anticancer properties, however, it causes systemic lymphopenia which impairs its use in cancer patients. In this study, we developed a nanoparticle (NP) combining docetaxel (DTX) and FTY for enhanced anticancer effect, targeted tumour delivery and reduced systemic toxicity. Methods: Docetaxel, FTY and glucosamine were covalently conjugated to poly(lactic-co-glycolic acid) (PLGA). NPs were characterised by dynamic light scattering and electron microscopy. The cellular uptake, cytotoxicity and in vivo antitumor efficacy of CNPs were evaluated. Results: We show for the first time that in triple negative breast cancer cells FTY provides chemosensitisation to DTX, allowing a four-fold reduction in the effective dose. We have encapsulated both drugs in PLGA complex NPs (CNPs), with narrow size distribution of ~ 100 nm and excellent cancer cell uptake providing sequential, sustained release of FTY and DTX. In triple negative breast cancer cells and mouse breast cancer models, CNPs had similar efficacy to systemic free therapies, but allowed an effective drug dose reduction. Application of CNPs has significantly reversed chemotherapy side effects such as weight loss, liver toxicity and, most notably, lymphopenia. Conclusions: We show for the first time the DTX chemosensitising effects of FTY in triple negative breast cancer. We further demonstrate that encapsulation of free drugs in CNPs can improve targeting, provide low off-target toxicity and most importantly reduce FTY-induced lymphopenia, offering potential therapeutic use of FTY in clinical cancer treatment

Reviewing the use of resilience concepts in forest sciences

Purpose of the review Resilience is a key concept to deal with an uncertain future in forestry. In recent years, it has received increasing attention from both research and practice. However, a common understanding of what resilience means in a forestry context, and how to operationalise it is lacking. Here, we conducted a systematic review of the recent forest science literature on resilience in the forestry context, synthesising how resilience is defined and assessed. Recent findings Based on a detailed review of 255 studies, we analysed how the concepts of engineering resilience, ecological resilience, and social-ecological resilience are used in forest sciences. A clear majority of the studies applied the concept of engineering resilience, quantifying resilience as the recovery time after a disturbance. The two most used indicators for engineering resilience were basal area increment and vegetation cover, whereas ecological resilience studies frequently focus on vegetation cover and tree density. In contrast, important social-ecological resilience indicators used in the literature are socio-economic diversity and stock of natural resources. In the context of global change, we expected an increase in studies adopting the more holistic social-ecological resilience concept, but this was not the observed trend. Summary Our analysis points to the nestedness of these three resilience concepts, suggesting that they are complementary rather than contradictory. It also means that the variety of resilience approaches does not need to be an obstacle for operationalisation of the concept. We provide guidance for choosing the most suitable resilience concept and indicators based on the management, disturbance and application context